Click here to switch to the map view.

The map label for this gene is gcvA [H]

Identifier: 238895051

GI number: 238895051

Start: 2936548

End: 2937432

Strand: Reverse

Name: gcvA [H]

Synonym: KP1_3083

Alternate gene names: 238895051

Gene position: 2937432-2936548 (Counterclockwise)

Preceding gene: 238895054

Following gene: 238895049

Centisome position: 55.97

GC content: 64.29

Gene sequence:

>885_bases
ATGCGTCGTAAAATCCCCAGCAGCGCTGCGCTACTGGCTTTTGAGGCCGCGGCTCGTCACGGCAACTTTGCCCGCGCGGC
GGCGGAGCTCGCGTTAACGGAAGGGGCCATCAGCCGGCAGATTGCCCGTCTGGAAGCGCTCCTCGACAGTAAACTCTTCG
ACAGGACGGGGAGCCGGGTGCGGCTCAATCCGGCAGGCGCCCGCTACGCTCGTCAGGTCCGCGAGATCCTCGCTCGTCTG
GAACGGGACACCCATGACGTCAGCGGGATGCCCGTCGATGGCCGCAGCCTGGAGATCGCCGTGCTCCCCACCTTCGCCAG
CCGCTGGTTAATTCCCCGGCTCGGGCGCTTTGCGGCGCAGCATCCGCATATCGTGGTCAATATCGCCGCCCGCAGCGATC
CCTTCATTCTGCCGGGAAGCGGATTTGATGCCGCCATCCATTTTGAACACCCGGCGTGGACCGGAATGGAGGTGACGTTT
TTATTTGCGGAGTATTTGCTGCCGGTCTGCCATGCTGCGCTGCTGACGGATGACGATCTTCCGGGGCTGCTTAACCGGCT
AACGCGCATTCACCGGCGGCAAAACCCGGACGCCTGGCTGCACTATGCCCGCGAGTGCGGTCTGGCGCTGGATAACCCCG
CTCAGGGGCCGCGCTACGATCTGCATGAGATGGCGATCGCCGCGGTACTTAGCCAGCAGGGTGTGGCACTGGTGCCGAAG
ATGTATGTGGAAAGCGAGCTGAGCGCCGGAACGCTGGTTGCCCCGTGGCCGGGCTCCCCGACGCTGGCTAAGCGCTTCTG
TTTGATCAAACCCGGCGGCGGGGAGGGGGAGCCAGCGCTGCAGATGTTTGAGCGCTGGCTGCAGACGGAGATTGCGGCAG
GTTAA

Upstream 100 bases:

>100_bases
ATCCTATCGGCTAAAAATGGCGCTGAAAAACGATGTTAATTCAAGGGTTAGTGATATTCTGTCACTGACTTTCGGTGAGC
CTGCACAAGGAGCATCGGCT

Downstream 100 bases:

>100_bases
GTCAGAGCTGGTCGGGAGACATTTAGCGCCGCCCGCTGGCGGGCATATTTGCCCGGCGATATCCCGGTATAACGGGTAAA
AGCTACGCTGAACGCGCTGG

Product: putative LysR-family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MRRKIPSSAALLAFEAAARHGNFARAAAELALTEGAISRQIARLEALLDSKLFDRTGSRVRLNPAGARYARQVREILARL
ERDTHDVSGMPVDGRSLEIAVLPTFASRWLIPRLGRFAAQHPHIVVNIAARSDPFILPGSGFDAAIHFEHPAWTGMEVTF
LFAEYLLPVCHAALLTDDDLPGLLNRLTRIHRRQNPDAWLHYARECGLALDNPAQGPRYDLHEMAIAAVLSQQGVALVPK
MYVESELSAGTLVAPWPGSPTLAKRFCLIKPGGGEGEPALQMFERWLQTEIAAG

Sequences:

>Translated_294_residues
MRRKIPSSAALLAFEAAARHGNFARAAAELALTEGAISRQIARLEALLDSKLFDRTGSRVRLNPAGARYARQVREILARL
ERDTHDVSGMPVDGRSLEIAVLPTFASRWLIPRLGRFAAQHPHIVVNIAARSDPFILPGSGFDAAIHFEHPAWTGMEVTF
LFAEYLLPVCHAALLTDDDLPGLLNRLTRIHRRQNPDAWLHYARECGLALDNPAQGPRYDLHEMAIAAVLSQQGVALVPK
MYVESELSAGTLVAPWPGSPTLAKRFCLIKPGGGEGEPALQMFERWLQTEIAAG
>Mature_294_residues
MRRKIPSSAALLAFEAAARHGNFARAAAELALTEGAISRQIARLEALLDSKLFDRTGSRVRLNPAGARYARQVREILARL
ERDTHDVSGMPVDGRSLEIAVLPTFASRWLIPRLGRFAAQHPHIVVNIAARSDPFILPGSGFDAAIHFEHPAWTGMEVTF
LFAEYLLPVCHAALLTDDDLPGLLNRLTRIHRRQNPDAWLHYARECGLALDNPAQGPRYDLHEMAIAAVLSQQGVALVPK
MYVESELSAGTLVAPWPGSPTLAKRFCLIKPGGGEGEPALQMFERWLQTEIAAG

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=296, Percent_Identity=35.1351351351351, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1788706, Length=292, Percent_Identity=29.4520547945205, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1786448, Length=286, Percent_Identity=32.1678321678322, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI145693193, Length=291, Percent_Identity=26.8041237113402, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI157672245, Length=126, Percent_Identity=38.8888888888889, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32274; Mature: 32274

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRKIPSSAALLAFEAAARHGNFARAAAELALTEGAISRQIARLEALLDSKLFDRTGSRV
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
RLNPAGARYARQVREILARLERDTHDVSGMPVDGRSLEIAVLPTFASRWLIPRLGRFAAQ
EECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHCC
HPHIVVNIAARSDPFILPGSGFDAAIHFEHPAWTGMEVTFLFAEYLLPVCHAALLTDDDL
CCEEEEEEEECCCCEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
PGLLNRLTRIHRRQNPDAWLHYARECGLALDNPAQGPRYDLHEMAIAAVLSQQGVALVPK
HHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEHHH
MYVESELSAGTLVAPWPGSPTLAKRFCLIKPGGGEGEPALQMFERWLQTEIAAG
HHHHHCCCCCEEEECCCCCHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRRKIPSSAALLAFEAAARHGNFARAAAELALTEGAISRQIARLEALLDSKLFDRTGSRV
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
RLNPAGARYARQVREILARLERDTHDVSGMPVDGRSLEIAVLPTFASRWLIPRLGRFAAQ
EECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHCC
HPHIVVNIAARSDPFILPGSGFDAAIHFEHPAWTGMEVTFLFAEYLLPVCHAALLTDDDL
CCEEEEEEEECCCCEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
PGLLNRLTRIHRRQNPDAWLHYARECGLALDNPAQGPRYDLHEMAIAAVLSQQGVALVPK
HHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEHHH
MYVESELSAGTLVAPWPGSPTLAKRFCLIKPGGGEGEPALQMFERWLQTEIAAG
HHHHHCCCCCEEEECCCCCHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]