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The map label for this gene is tyrS [H]

Identifier: 238895018

GI number: 238895018

Start: 2904720

End: 2905994

Strand: Reverse

Name: tyrS [H]

Synonym: KP1_3047

Alternate gene names: 238895018

Gene position: 2905994-2904720 (Counterclockwise)

Preceding gene: 238895019

Following gene: 238895017

Centisome position: 55.37

GC content: 55.53

Gene sequence:

>1275_bases
ATGGCAAGCAGTAACTTGATTAAACAATTGCAAGAGCGGGGGCTGGTAGCCCAGGTGACGGACGAGGAAGCGTTAGCAGA
GCGACTGGCGCAAGGCCCGATCGCGCTCTATTGCGGCTTCGATCCTACCGCTGACAGCTTGCATTTGGGGCATCTTGTTC
CATTGTTATGCCTGAAACGCTTCCAGCAGGCAGGTCATAAACCTGTGGCGCTGGTGGGCGGCGCAACCGGTCTGATTGGC
GACCCAAGCTTCAAAGCCGCTGAGCGTAAACTAAACACCGAGGATACCGTGCAGGAGTGGGTGGATAAAATCCGCAAACA
GGTGGCTCCGTTCCTTGATTTTGACTGCGGCGACAACTCGGCGATCGCGGCGAATAACTATGACTGGTTTGGCAGCATGA
ACGTGCTGACCTTCCTGCGCGATATCGGTAAACACTTCTCTGTTAACCAGATGATCAACAAAGAAGCGGTGAAGCAGCGT
CTGAATCGTGACGATCAGGGTATCTCCTTTACCGAGTTCTCTTACAATCTGCTGCAGGGTTACGACTTTGCCTGCCTGAA
CAAGCTGCACGGCGTGGCGCTGCAGATTGGCGGCTCTGACCAGTGGGGCAACATCACCTCCGGCATCGATCTGACCCGTC
GTCTGCATCAGAACCAGGTGTTTGGCCTGACCGTACCGCTGATCACCAAAGCGGACGGCACCAAATTCGGTAAAACCGAA
GGCGGCGCCGTGTGGCTGGATCCGAAGAAAACCAGTCCGTACAAATTCTACCAGTTCTGGATCAACACCGCGGATGCTGA
TGTCTATCGCTTCCTCAAATTCTTCACCTTTATGGACATTGTGGAGATCAATGCCCTGGAAGAAGAAGACAAAAACAGCG
GTAAAGCGCCGCGCGCCCAGTACGTGCTGGCAGAGCAGGTGACGCGCCTTGTCCACGGCGAAGAAGGCCTGGAAGCGGCG
AAGCGCATTACCGAAAGCCTGTTCAACGGTAACCTGAGCGACCTGAGCGAAGCAGACTTCGAACAGCTGGCCCAGGATGG
CGTGCCGATGATCGAAATGGAAAAAGGCGCCGATCTGCTGCAGGCGCTGGTTGATTCCGAGCTGCAGCCGTCCCGCGGTC
AGGCGCGTAAAACCGTTGCCTCTAACGCGGTGACCATCAACGGCGAAAAACAGGCCGACCCGGAATATGTTTTCGCCGAC
AGCGATCGCCTGTTTGGCCGCTACACCTTGCTGCGTCGCGGTAAGAAAAATTACTGCCTGGTCTGCTGGAAATAA

Upstream 100 bases:

>100_bases
CTGGCGCTGAAAGGGCCGGCGCTTTATTCTATGTACTTTTCGCATCTGGCGAAAAGTCGTGTACCGGCAGAGGTGCAGTC
GTTTATACATGGAGAATTTG

Downstream 100 bases:

>100_bases
AAAAATACTGCCAGGGCGTGGGAAACCACGCCCTTTTTCTTTTTAGGGTTGTGGTAAGAAAATGAAGAACATCCTCGCCA
TTCAGTCTCACGTTGTGTTT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTADSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIG
DPSFKAAERKLNTEDTVQEWVDKIRKQVAPFLDFDCGDNSAIAANNYDWFGSMNVLTFLRDIGKHFSVNQMINKEAVKQR
LNRDDQGISFTEFSYNLLQGYDFACLNKLHGVALQIGGSDQWGNITSGIDLTRRLHQNQVFGLTVPLITKADGTKFGKTE
GGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFTFMDIVEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLEAA
KRITESLFNGNLSDLSEADFEQLAQDGVPMIEMEKGADLLQALVDSELQPSRGQARKTVASNAVTINGEKQADPEYVFAD
SDRLFGRYTLLRRGKKNYCLVCWK

Sequences:

>Translated_424_residues
MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTADSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIG
DPSFKAAERKLNTEDTVQEWVDKIRKQVAPFLDFDCGDNSAIAANNYDWFGSMNVLTFLRDIGKHFSVNQMINKEAVKQR
LNRDDQGISFTEFSYNLLQGYDFACLNKLHGVALQIGGSDQWGNITSGIDLTRRLHQNQVFGLTVPLITKADGTKFGKTE
GGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFTFMDIVEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLEAA
KRITESLFNGNLSDLSEADFEQLAQDGVPMIEMEKGADLLQALVDSELQPSRGQARKTVASNAVTINGEKQADPEYVFAD
SDRLFGRYTLLRRGKKNYCLVCWK
>Mature_423_residues
ASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTADSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGD
PSFKAAERKLNTEDTVQEWVDKIRKQVAPFLDFDCGDNSAIAANNYDWFGSMNVLTFLRDIGKHFSVNQMINKEAVKQRL
NRDDQGISFTEFSYNLLQGYDFACLNKLHGVALQIGGSDQWGNITSGIDLTRRLHQNQVFGLTVPLITKADGTKFGKTEG
GAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFTFMDIVEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLEAAK
RITESLFNGNLSDLSEADFEQLAQDGVPMIEMEKGADLLQALVDSELQPSRGQARKTVASNAVTINGEKQADPEYVFADS
DRLFGRYTLLRRGKKNYCLVCWK

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=407, Percent_Identity=36.3636363636364, Blast_Score=249, Evalue=4e-66,
Organism=Escherichia coli, GI1787925, Length=424, Percent_Identity=94.1037735849057, Blast_Score=840, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25145802, Length=409, Percent_Identity=36.4303178484108, Blast_Score=236, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI25145805, Length=333, Percent_Identity=34.5345345345345, Blast_Score=170, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6325160, Length=438, Percent_Identity=35.8447488584475, Blast_Score=269, Evalue=7e-73,
Organism=Drosophila melanogaster, GI20130383, Length=445, Percent_Identity=37.0786516853933, Blast_Score=299, Evalue=3e-81,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 47263; Mature: 47132

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTADSLHLGHLVPLLCLKR
CCCHHHHHHHHHCCCEEEECCHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHH
FQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEDTVQEWVDKIRKQVAPFLDFDCGDNS
HHHCCCCCEEEECCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
AIAANNYDWFGSMNVLTFLRDIGKHFSVNQMINKEAVKQRLNRDDQGISFTEFSYNLLQG
EEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHC
YDFACLNKLHGVALQIGGSDQWGNITSGIDLTRRLHQNQVFGLTVPLITKADGTKFGKTE
CCHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCC
GGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFTFMDIVEINALEEEDKNSGKAPRAQ
CCEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
YVLAEQVTRLVHGEEGLEAAKRITESLFNGNLSDLSEADFEQLAQDGVPMIEMEKGADLL
HHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCHHHH
QALVDSELQPSRGQARKTVASNAVTINGEKQADPEYVFADSDRLFGRYTLLRRGKKNYCL
HHHHCCCCCCCCCHHHHHHHCCCEEECCCCCCCCCEEEECCCHHHHHHHHHHCCCCCEEE
VCWK
EEEC
>Mature Secondary Structure 
ASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTADSLHLGHLVPLLCLKR
CCHHHHHHHHHCCCEEEECCHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHH
FQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEDTVQEWVDKIRKQVAPFLDFDCGDNS
HHHCCCCCEEEECCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
AIAANNYDWFGSMNVLTFLRDIGKHFSVNQMINKEAVKQRLNRDDQGISFTEFSYNLLQG
EEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHC
YDFACLNKLHGVALQIGGSDQWGNITSGIDLTRRLHQNQVFGLTVPLITKADGTKFGKTE
CCHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCC
GGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFTFMDIVEINALEEEDKNSGKAPRAQ
CCEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
YVLAEQVTRLVHGEEGLEAAKRITESLFNGNLSDLSEADFEQLAQDGVPMIEMEKGADLL
HHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCHHHH
QALVDSELQPSRGQARKTVASNAVTINGEKQADPEYVFADSDRLFGRYTLLRRGKKNYCL
HHHHCCCCCCCCCHHHHHHHCCCEEECCCCCCCCCEEEECCCHHHHHHHHHHCCCCCEEE
VCWK
EEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA