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The map label for this gene is frwB [H]

Identifier: 238894779

GI number: 238894779

Start: 2661353

End: 2661664

Strand: Reverse

Name: frwB [H]

Synonym: KP1_2788

Alternate gene names: 238894779

Gene position: 2661664-2661353 (Counterclockwise)

Preceding gene: 238894780

Following gene: 238894778

Centisome position: 50.71

GC content: 45.83

Gene sequence:

>312_bases
ATGAATATTGTCGGCGTTACCGCCTGCACGGTGGGGATTGCACATACCTATATTGCGCAAAAGAAAATTGAAACTGCGGC
TAAAAAAGCCGGACATAACGTTAAAATCGAGACGCAAGGAACCATCGGCATTGAGAACCCGTTAACCGCTGATGAGATTG
CCGCAGCAGATATTGTCCTGCTTGCTGCCGATGTCAAAGTAACGGGCGAGGAGAGATTTGCCGGCAAAAAAGTCGTTAAA
GTCGCGACGGAAACGGCAGTCAAATCCCCCAATAAGTTGATTGAAAAACTCAGTGAAATAGTCAATTCATAA

Upstream 100 bases:

>100_bases
TCATTGCGCTTTTCAATTCAGAAACTGAAAACGCCGATGCTTAATACCAACTTGCTAATATCGATTTGTAATTTATTAAA
TTAATTAACGGAGAAACATT

Downstream 100 bases:

>100_bases
TTTTATTTCGGCCTGGCGACTCTACATTAAAGGGCGTGACTTATGTCTCTAAAACAAATATGGCAAGCAGCAAACCCTAA
AGGACATCTGCTGACCGCTA

Product: fructose-mannitol-specific PTS family enzyme IIB component

Products: NA

Alternate protein names: PTS system fructose-like EIIB component 2 [H]

Number of amino acids: Translated: 103; Mature: 103

Protein sequence:

>103_residues
MNIVGVTACTVGIAHTYIAQKKIETAAKKAGHNVKIETQGTIGIENPLTADEIAAADIVLLAADVKVTGEERFAGKKVVK
VATETAVKSPNKLIEKLSEIVNS

Sequences:

>Translated_103_residues
MNIVGVTACTVGIAHTYIAQKKIETAAKKAGHNVKIETQGTIGIENPLTADEIAAADIVLLAADVKVTGEERFAGKKVVK
VATETAVKSPNKLIEKLSEIVNS
>Mature_103_residues
MNIVGVTACTVGIAHTYIAQKKIETAAKKAGHNVKIETQGTIGIENPLTADEIAAADIVLLAADVKVTGEERFAGKKVVK
VATETAVKSPNKLIEKLSEIVNS

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: COG1445

COG function: function code G; Phosphotransferase system fructose-specific component IIB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1790387, Length=95, Percent_Identity=50.5263157894737, Blast_Score=103, Evalue=2e-24,
Organism=Escherichia coli, GI87082348, Length=99, Percent_Identity=44.4444444444444, Blast_Score=92, Evalue=9e-21,
Organism=Escherichia coli, GI1788492, Length=95, Percent_Identity=45.2631578947368, Blast_Score=85, Evalue=7e-19,
Organism=Escherichia coli, GI1788730, Length=101, Percent_Identity=38.6138613861386, Blast_Score=80, Evalue=3e-17,
Organism=Escherichia coli, GI1790390, Length=101, Percent_Identity=33.6633663366337, Blast_Score=77, Evalue=2e-16,
Organism=Escherichia coli, GI1786951, Length=94, Percent_Identity=42.5531914893617, Blast_Score=75, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003353 [H]

Pfam domain/function: PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 10798; Mature: 10798

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS51099 PTS_EIIB_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIVGVTACTVGIAHTYIAQKKIETAAKKAGHNVKIETQGTIGIENPLTADEIAAADIVL
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHEEE
LAADVKVTGEERFAGKKVVKVATETAVKSPNKLIEKLSEIVNS
EEECEEECCCCHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MNIVGVTACTVGIAHTYIAQKKIETAAKKAGHNVKIETQGTIGIENPLTADEIAAADIVL
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHEEE
LAADVKVTGEERFAGKKVVKVATETAVKSPNKLIEKLSEIVNS
EEECEEECCCCHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]