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The map label for this gene is purR [H]

Identifier: 238894766

GI number: 238894766

Start: 2648444

End: 2649466

Strand: Direct

Name: purR [H]

Synonym: KP1_2774

Alternate gene names: 238894766

Gene position: 2648444-2649466 (Clockwise)

Preceding gene: 238894764

Following gene: 238894767

Centisome position: 50.46

GC content: 59.63

Gene sequence:

>1023_bases
ATGGCGCAGGAAATGAGTATAAAACGTGTATTGTTATCCGATGTGGCAAAGCTGGCCGGGCTGTCGAAGGCGACCCTGTC
GCGCTATATGAACAACAGTATTGTGCTGCCGCAGGACACCATCGACCGCATCGAAACCGCCATTCGCGAACTGGACTATC
GCGGCAATAGCCTGGCACGCCGTCTTAGCAAAGGCGGCAGCGAAACGCTTGGCCTGGTGCTTCCCGATATTACCAACCCC
TTCTTTGCCGAACTGGCCGACGCAGCCGAAGAGGCCGCTTCTGCCAGCGGCTACAGCCTGGTGCTGTGTATCACCCGCAA
CAACCCGGAAAAAGAGTGCCAGTTCATTCGCTGGCTGGATACCTGCCAGGTCGACGGTCTGCTGTTCACCACCAACCGCC
CCGATAATGGCCTGCTGCGCAAGGAAGTCCAGCGTCACGAACGGATTGTCCTGCTGGATGAAGATATTCCCGGCAGTAAG
GTACCGAAAGTGTTTGCCGATAACGTCCAGGGCGGGCGGATCGCCACCGAAAAACTGATCGCCGCCGGGCATCGCCATAT
CGCTTTCGTCGGTGGCCCGGACAAGCTGATGAGCGTCCGCGAGCGCTATCAGGGCTTTTGCACCGCGATGGAGCAAGCGG
GCCTGAGCTGGCCGCCCGAATGGGTGATGTATGGCGACTACCAGCGCGAGTTTGGCCAGCAAGCGTTGCGGTACCTGTTC
AGCCAACAGGTTCGCCCTACCGCCGTCTTCGCTGCCAGCGACTATCTGGTGCTCGGTCTGCTCGACGGCTTGCGCGCCAG
TGGGCTCCAGGCGCCAGAGGCGCTATCGTTAGTCGGCTTCGATGACGCCAACTATGCCGATTTTACCCAGCCGCGGATCT
CCACTATCCGTCAGCCCGCCCGCGAACTGGGCCGCACTGCGGTGAACATCATGATGCGCCTGCTAAACGACGATCCGGAT
ATCCCTGCGGAAACCCGCCTGCCGGTTGAATGGATTGGCCGCGACTCGATCAAGATTTGTTAA

Upstream 100 bases:

>100_bases
GATTAAAATTTGTCAAGCATTTATTTTTGGTGATTGTTCGCAGAGAAATTGCTGTATCCACCGCGCTTAACTATGTTACA
GATAGCCGATTCTGGCAGAA

Downstream 100 bases:

>100_bases
GCCCGTCCCAAAATGGCGCAAAAGCGCCCGCTTTTGGCGCAAAAAACGATCAATTAACAATTTATTCACTATAATCAGTA
AACCGGTTTACCGGATTGTA

Product: periplasmic binding protein/LacI transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MAQEMSIKRVLLSDVAKLAGLSKATLSRYMNNSIVLPQDTIDRIETAIRELDYRGNSLARRLSKGGSETLGLVLPDITNP
FFAELADAAEEAASASGYSLVLCITRNNPEKECQFIRWLDTCQVDGLLFTTNRPDNGLLRKEVQRHERIVLLDEDIPGSK
VPKVFADNVQGGRIATEKLIAAGHRHIAFVGGPDKLMSVRERYQGFCTAMEQAGLSWPPEWVMYGDYQREFGQQALRYLF
SQQVRPTAVFAASDYLVLGLLDGLRASGLQAPEALSLVGFDDANYADFTQPRISTIRQPARELGRTAVNIMMRLLNDDPD
IPAETRLPVEWIGRDSIKIC

Sequences:

>Translated_340_residues
MAQEMSIKRVLLSDVAKLAGLSKATLSRYMNNSIVLPQDTIDRIETAIRELDYRGNSLARRLSKGGSETLGLVLPDITNP
FFAELADAAEEAASASGYSLVLCITRNNPEKECQFIRWLDTCQVDGLLFTTNRPDNGLLRKEVQRHERIVLLDEDIPGSK
VPKVFADNVQGGRIATEKLIAAGHRHIAFVGGPDKLMSVRERYQGFCTAMEQAGLSWPPEWVMYGDYQREFGQQALRYLF
SQQVRPTAVFAASDYLVLGLLDGLRASGLQAPEALSLVGFDDANYADFTQPRISTIRQPARELGRTAVNIMMRLLNDDPD
IPAETRLPVEWIGRDSIKIC
>Mature_339_residues
AQEMSIKRVLLSDVAKLAGLSKATLSRYMNNSIVLPQDTIDRIETAIRELDYRGNSLARRLSKGGSETLGLVLPDITNPF
FAELADAAEEAASASGYSLVLCITRNNPEKECQFIRWLDTCQVDGLLFTTNRPDNGLLRKEVQRHERIVLLDEDIPGSKV
PKVFADNVQGGRIATEKLIAAGHRHIAFVGGPDKLMSVRERYQGFCTAMEQAGLSWPPEWVMYGDYQREFGQQALRYLFS
QQVRPTAVFAASDYLVLGLLDGLRASGLQAPEALSLVGFDDANYADFTQPRISTIRQPARELGRTAVNIMMRLLNDDPDI
PAETRLPVEWIGRDSIKIC

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=308, Percent_Identity=34.4155844155844, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1787948, Length=328, Percent_Identity=32.0121951219512, Blast_Score=177, Evalue=7e-46,
Organism=Escherichia coli, GI1790369, Length=303, Percent_Identity=33.003300330033, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1788474, Length=304, Percent_Identity=29.2763157894737, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1789202, Length=288, Percent_Identity=29.8611111111111, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1789068, Length=327, Percent_Identity=29.0519877675841, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1787580, Length=314, Percent_Identity=25.796178343949, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1787906, Length=327, Percent_Identity=27.82874617737, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI48994940, Length=303, Percent_Identity=29.042904290429, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1786540, Length=331, Percent_Identity=25.9818731117825, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1786268, Length=318, Percent_Identity=26.1006289308176, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1790715, Length=320, Percent_Identity=25.3125, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1789456, Length=328, Percent_Identity=24.6951219512195, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790192, Length=273, Percent_Identity=27.4725274725275, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1790689, Length=311, Percent_Identity=24.1157556270096, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37849; Mature: 37718

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS50932 HTH_LACI_2 ; PS00626 RCC1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQEMSIKRVLLSDVAKLAGLSKATLSRYMNNSIVLPQDTIDRIETAIRELDYRGNSLAR
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEECCHHHHHHHHHHHHHHCCCCHHHHH
RLSKGGSETLGLVLPDITNPFFAELADAAEEAASASGYSLVLCITRNNPEKECQFIRWLD
HHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHH
TCQVDGLLFTTNRPDNGLLRKEVQRHERIVLLDEDIPGSKVPKVFADNVQGGRIATEKLI
HHCCCCEEEECCCCCCCHHHHHHHHCCEEEEEECCCCCCCCCHHHHCCCCCCCHHHHHHH
AAGHRHIAFVGGPDKLMSVRERYQGFCTAMEQAGLSWPPEWVMYGDYQREFGQQALRYLF
HCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHH
SQQVRPTAVFAASDYLVLGLLDGLRASGLQAPEALSLVGFDDANYADFTQPRISTIRQPA
HCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHH
RELGRTAVNIMMRLLNDDPDIPAETRLPVEWIGRDSIKIC
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCEECC
>Mature Secondary Structure 
AQEMSIKRVLLSDVAKLAGLSKATLSRYMNNSIVLPQDTIDRIETAIRELDYRGNSLAR
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEECCHHHHHHHHHHHHHHCCCCHHHHH
RLSKGGSETLGLVLPDITNPFFAELADAAEEAASASGYSLVLCITRNNPEKECQFIRWLD
HHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHH
TCQVDGLLFTTNRPDNGLLRKEVQRHERIVLLDEDIPGSKVPKVFADNVQGGRIATEKLI
HHCCCCEEEECCCCCCCHHHHHHHHCCEEEEEECCCCCCCCCHHHHCCCCCCCHHHHHHH
AAGHRHIAFVGGPDKLMSVRERYQGFCTAMEQAGLSWPPEWVMYGDYQREFGQQALRYLF
HCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHH
SQQVRPTAVFAASDYLVLGLLDGLRASGLQAPEALSLVGFDDANYADFTQPRISTIRQPA
HCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHH
RELGRTAVNIMMRLLNDDPDIPAETRLPVEWIGRDSIKIC
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA