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The map label for this gene is ydgB [H]

Identifier: 238894538

GI number: 238894538

Start: 2435822

End: 2436544

Strand: Reverse

Name: ydgB [H]

Synonym: KP1_2533

Alternate gene names: 238894538

Gene position: 2436544-2435822 (Counterclockwise)

Preceding gene: 238894539

Following gene: 238894536

Centisome position: 46.42

GC content: 60.86

Gene sequence:

>723_bases
ATGGCTGAACAACAGCCCCGCCCGATTCTGATCACAGGAGCAGGCCGCCGCATCGGCCTCGCTCTGGCACACCATTTTCT
GCAGCAGCGCCAGCCGGTTATCGTCAGCTATCGTACGCCCTATCCGGCGATTGACGGCCTGCGCGAAGCGGGCGCGCTCT
GCCTGCAGGCCGATTTTAGCAGCGATGACGGCATTCTGACCTTCGCTGAAGCGGTCAAATCACACACCGACGGTCTGCGC
GCCATCATCCATAACGCCAGCGACTGGATGGCGGAAAAGCCGGGCGTCCCGCTGAGCGCCGTCATCAACCGCATGATGCA
GATCCACGTCCACGCCCCCTATCTGCTCAACCATGCGCTGGAGGCGTTGCTGCGCGGCCACGGCCATGCCGCCAGCGACA
TTATCCATATCACCGATTACGTGGTGGAGCGCGGGAGCGACAAACATATCGCCTATGCCGCCAGCAAAGCCGCCCTGGAT
AACATGACCCGCTCCTTCGCTCGCAAGCTGGCGCCAGAGGTTAAGGTCAACGCCATCGCGCCGTCGCTAATCATGTTTAA
TGAGGGTGACGACGAGGCCTATCGTCAGCAGGCGCTGGATAAATCGCTGATGAAAATCGCCCCTGGCGAAAAAGAGATTA
GCGATCTCATCGATTACCTGTTTACCAGCCGCTACGTTACCGGCCGCTCATTCGCTGTCGACGGCGGCCGCCCGCTGCGC
TAG

Upstream 100 bases:

>100_bases
AGTGGCGCTAATTGGTTTATTGCTGGTTGCCGCTTTGCCGGCAACGTGGATGCTGGTGGGGTAAGCGGTCGCCCTGCCGC
AGAACGCATAGGAGATACCC

Downstream 100 bases:

>100_bases
TGGAAACGGCTCCAGCCGAGGATTAACAGCCAGGCGCACAGCCCCCAGCATCCCACTGCGCCTGCCAGCGCCAACGGCAG
GCGCATGATCCCGGTGAAAT

Product: short chain dehydrogenase

Products: NA

Alternate protein names: DHFR [H]

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MAEQQPRPILITGAGRRIGLALAHHFLQQRQPVIVSYRTPYPAIDGLREAGALCLQADFSSDDGILTFAEAVKSHTDGLR
AIIHNASDWMAEKPGVPLSAVINRMMQIHVHAPYLLNHALEALLRGHGHAASDIIHITDYVVERGSDKHIAYAASKAALD
NMTRSFARKLAPEVKVNAIAPSLIMFNEGDDEAYRQQALDKSLMKIAPGEKEISDLIDYLFTSRYVTGRSFAVDGGRPLR

Sequences:

>Translated_240_residues
MAEQQPRPILITGAGRRIGLALAHHFLQQRQPVIVSYRTPYPAIDGLREAGALCLQADFSSDDGILTFAEAVKSHTDGLR
AIIHNASDWMAEKPGVPLSAVINRMMQIHVHAPYLLNHALEALLRGHGHAASDIIHITDYVVERGSDKHIAYAASKAALD
NMTRSFARKLAPEVKVNAIAPSLIMFNEGDDEAYRQQALDKSLMKIAPGEKEISDLIDYLFTSRYVTGRSFAVDGGRPLR
>Mature_239_residues
AEQQPRPILITGAGRRIGLALAHHFLQQRQPVIVSYRTPYPAIDGLREAGALCLQADFSSDDGILTFAEAVKSHTDGLRA
IIHNASDWMAEKPGVPLSAVINRMMQIHVHAPYLLNHALEALLRGHGHAASDIIHITDYVVERGSDKHIAYAASKAALDN
MTRSFARKLAPEVKVNAIAPSLIMFNEGDDEAYRQQALDKSLMKIAPGEKEISDLIDYLFTSRYVTGRSFAVDGGRPLR

Specific function: Catalyzes the reduction of dihydrofolate to tetrahydrofolate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FolM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787891, Length=240, Percent_Identity=77.9166666666667, Blast_Score=387, Evalue=1e-109,
Organism=Escherichia coli, GI2367175, Length=243, Percent_Identity=26.3374485596708, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 26389; Mature: 26257

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEQQPRPILITGAGRRIGLALAHHFLQQRQPVIVSYRTPYPAIDGLREAGALCLQADFS
CCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCEEEEECCC
SDDGILTFAEAVKSHTDGLRAIIHNASDWMAEKPGVPLSAVINRMMQIHVHAPYLLNHAL
CCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHH
EALLRGHGHAASDIIHITDYVVERGSDKHIAYAASKAALDNMTRSFARKLAPEVKVNAIA
HHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
PSLIMFNEGDDEAYRQQALDKSLMKIAPGEKEISDLIDYLFTSRYVTGRSFAVDGGRPLR
CEEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCC
>Mature Secondary Structure 
AEQQPRPILITGAGRRIGLALAHHFLQQRQPVIVSYRTPYPAIDGLREAGALCLQADFS
CCCCCCCEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCEEEEECCC
SDDGILTFAEAVKSHTDGLRAIIHNASDWMAEKPGVPLSAVINRMMQIHVHAPYLLNHAL
CCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHH
EALLRGHGHAASDIIHITDYVVERGSDKHIAYAASKAALDNMTRSFARKLAPEVKVNAIA
HHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
PSLIMFNEGDDEAYRQQALDKSLMKIAPGEKEISDLIDYLFTSRYVTGRSFAVDGGRPLR
CEEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA