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The map label for this gene is rstB [H]

Identifier: 238894534

GI number: 238894534

Start: 2432479

End: 2433780

Strand: Reverse

Name: rstB [H]

Synonym: KP1_2528

Alternate gene names: 238894534

Gene position: 2433780-2432479 (Counterclockwise)

Preceding gene: 238894535

Following gene: 238894533

Centisome position: 46.37

GC content: 59.75

Gene sequence:

>1302_bases
ATGAAAAAGCTGTTCGTGCAGTTCTATCTTCTGCTCTTCGTCTGCTTTCTGGTGATGACCATGCTGGTCGGTCTGGTCTA
TAAATTTACCGCGGAGCGCGCCGGACGGCAGTCGCTTGACGATTTGATGAAAAGCTCGCTGTATCTGATGCGCAGCGAGC
TGCGGGAGATACCGCCGCGCGACTGGAGTAAGACCCTTAAAGAGCTGGACCTGAATTTATCGTTTGATCTGCACATCGAG
CCGATCAGCAAATTTAAGCTGTCGGAAAGCTCGATGCAGCACCTGCGCGCCGGCGATATCGTCGCCCTCGACGATCAGTA
CACCTTTATTCAGCGCATTCCCCGCAGCCACTATGTGCTGTCGGTGGGCCCGGTGCCCTATCTCTATTTTCTGCATGAGA
TGCGCCTGCTGGACGTGGCGCTGATGGCGTTTATTGCTATCTCTCTGGCCTTTCCGGTGTTTATCTGGATGCGCCCGCAC
TGGCAGGACATGCTGCGCATTGAGACGGCAGCCCAGCGCTTTGGCGAAGGTCACCTCAGCGAGCGCATTCACTTCGACAG
TATGTCCAGCTTCGAGCGGCTCGGGGTAGCCTTTAACCAAATGGCCGATAACATTAATGCGCTTATTGCCAGCAAAAAGC
AACTGATTGACGGCATCGCCCATGAACTCAGGACACCGCTGGTACGCCTGCGCTACCGTCTGGAGATGAGCGAGAATCTC
AGCGAGGCGGAATCGCAGGCGCTGAACCGCGATATCGGTCAGCTGGAGGCGCTGATTGAAGAGCTCCTGACCTACGCCCG
CCTCGACCGCCCGCAGACGGAGCTCAGCCTGACGACTCCCGATTTTCCCGCCTGGATCAGCGATCACGTCGAGGATATCC
AGATGGTTAACCCACAGCGCGAGGTCGCCCTGGCGACGCTGACCCACGGTAACTACGGCGCGCTGGATATGCGACTGATG
GAGCGGGTGCTGGATAATTTGGTCAACAACGCCCTGCGCTACAGCAGCCAGCGGGTGGCGGTCAGCCTGACGCTGCAGGG
TTCGCGCGCATCGTTGCTGGTTGAGGATGACGGCCCGGGAATCGCCCCCGAGGAGCGCGAGCGGGTGTTTGAGCCCTTTG
TCCGCCTTGACCCCAGCCGGGACCGGGCGACAGGAGGCTGCGGCCTCGGCCTGGCGATTGTCCACAGTATCGCCCAGGCG
ATGGGCGGCGAGGTGCGCTGCGAGGCCAGCCCTCTCGGCGGCGCCCGCTTCTGCTTTAGCTGGCCGGTCTATCATCAGCT
GCCTGATTTCACCTCTGCCTGA

Upstream 100 bases:

>100_bases
CCCGCGCCTCGCCCGGGGATATCGATAACATTGACTCCCAGTCGCGCAGAAGATGCCCCTGGGGCGCCAGACAGGTTTCT
CTTGAATACGCGGTAATGTA

Downstream 100 bases:

>100_bases
ATAAAACTTGCGCAGAAGCCGCGGGCTGGGTATGCTGCAAAAATCTAATAGTATGTTGTAACTAAGGTGGTAATAATGGC
CAGCTACGACCTCGTTGAAC

Product: sensor protein RstB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MKKLFVQFYLLLFVCFLVMTMLVGLVYKFTAERAGRQSLDDLMKSSLYLMRSELREIPPRDWSKTLKELDLNLSFDLHIE
PISKFKLSESSMQHLRAGDIVALDDQYTFIQRIPRSHYVLSVGPVPYLYFLHEMRLLDVALMAFIAISLAFPVFIWMRPH
WQDMLRIETAAQRFGEGHLSERIHFDSMSSFERLGVAFNQMADNINALIASKKQLIDGIAHELRTPLVRLRYRLEMSENL
SEAESQALNRDIGQLEALIEELLTYARLDRPQTELSLTTPDFPAWISDHVEDIQMVNPQREVALATLTHGNYGALDMRLM
ERVLDNLVNNALRYSSQRVAVSLTLQGSRASLLVEDDGPGIAPEERERVFEPFVRLDPSRDRATGGCGLGLAIVHSIAQA
MGGEVRCEASPLGGARFCFSWPVYHQLPDFTSA

Sequences:

>Translated_433_residues
MKKLFVQFYLLLFVCFLVMTMLVGLVYKFTAERAGRQSLDDLMKSSLYLMRSELREIPPRDWSKTLKELDLNLSFDLHIE
PISKFKLSESSMQHLRAGDIVALDDQYTFIQRIPRSHYVLSVGPVPYLYFLHEMRLLDVALMAFIAISLAFPVFIWMRPH
WQDMLRIETAAQRFGEGHLSERIHFDSMSSFERLGVAFNQMADNINALIASKKQLIDGIAHELRTPLVRLRYRLEMSENL
SEAESQALNRDIGQLEALIEELLTYARLDRPQTELSLTTPDFPAWISDHVEDIQMVNPQREVALATLTHGNYGALDMRLM
ERVLDNLVNNALRYSSQRVAVSLTLQGSRASLLVEDDGPGIAPEERERVFEPFVRLDPSRDRATGGCGLGLAIVHSIAQA
MGGEVRCEASPLGGARFCFSWPVYHQLPDFTSA
>Mature_433_residues
MKKLFVQFYLLLFVCFLVMTMLVGLVYKFTAERAGRQSLDDLMKSSLYLMRSELREIPPRDWSKTLKELDLNLSFDLHIE
PISKFKLSESSMQHLRAGDIVALDDQYTFIQRIPRSHYVLSVGPVPYLYFLHEMRLLDVALMAFIAISLAFPVFIWMRPH
WQDMLRIETAAQRFGEGHLSERIHFDSMSSFERLGVAFNQMADNINALIASKKQLIDGIAHELRTPLVRLRYRLEMSENL
SEAESQALNRDIGQLEALIEELLTYARLDRPQTELSLTTPDFPAWISDHVEDIQMVNPQREVALATLTHGNYGALDMRLM
ERVLDNLVNNALRYSSQRVAVSLTLQGSRASLLVEDDGPGIAPEERERVFEPFVRLDPSRDRATGGCGLGLAIVHSIAQA
MGGEVRCEASPLGGARFCFSWPVYHQLPDFTSA

Specific function: Member of the two-component regulatory system rstB/rstA. RstB functions as a membrane-associated protein kinase that phosphorylates rstA (Potential) [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=433, Percent_Identity=80.1385681293303, Blast_Score=733, Evalue=0.0,
Organism=Escherichia coli, GI1790346, Length=272, Percent_Identity=33.8235294117647, Blast_Score=144, Evalue=8e-36,
Organism=Escherichia coli, GI1789808, Length=265, Percent_Identity=31.3207547169811, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1786783, Length=307, Percent_Identity=26.0586319218241, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786600, Length=226, Percent_Identity=28.3185840707965, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788393, Length=285, Percent_Identity=27.0175438596491, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789403, Length=234, Percent_Identity=31.1965811965812, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1788279, Length=273, Percent_Identity=26.007326007326, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87082128, Length=259, Percent_Identity=27.7992277992278, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081816, Length=109, Percent_Identity=37.6146788990826, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI145693157, Length=255, Percent_Identity=24.7058823529412, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1788713, Length=234, Percent_Identity=26.0683760683761, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786912, Length=232, Percent_Identity=28.0172413793103, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790436, Length=219, Percent_Identity=27.8538812785388, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788549, Length=229, Percent_Identity=25.764192139738, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790551, Length=222, Percent_Identity=27.9279279279279, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789149, Length=233, Percent_Identity=27.0386266094421, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790861, Length=211, Percent_Identity=29.3838862559242, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI48994928, Length=221, Percent_Identity=25.3393665158371, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 49313; Mature: 49313

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLFVQFYLLLFVCFLVMTMLVGLVYKFTAERAGRQSLDDLMKSSLYLMRSELREIPPR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC
DWSKTLKELDLNLSFDLHIEPISKFKLSESSMQHLRAGDIVALDDQYTFIQRIPRSHYVL
CHHHHHHHHCCCCEEEEEECCHHHHHCCHHHHHHHCCCCEEEECCCHHHHHHCCCCCEEE
SVGPVPYLYFLHEMRLLDVALMAFIAISLAFPVFIWMRPHWQDMLRIETAAQRFGEGHLS
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHH
ERIHFDSMSSFERLGVAFNQMADNINALIASKKQLIDGIAHELRTPLVRLRYRLEMSENL
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
SEAESQALNRDIGQLEALIEELLTYARLDRPQTELSLTTPDFPAWISDHVEDIQMVNPQR
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCH
EVALATLTHGNYGALDMRLMERVLDNLVNNALRYSSQRVAVSLTLQGSRASLLVEDDGPG
HEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCEEEEEECCCCC
IAPEERERVFEPFVRLDPSRDRATGGCGLGLAIVHSIAQAMGGEVRCEASPLGGARFCFS
CCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEE
WPVYHQLPDFTSA
CCHHHHCCCCCCC
>Mature Secondary Structure
MKKLFVQFYLLLFVCFLVMTMLVGLVYKFTAERAGRQSLDDLMKSSLYLMRSELREIPPR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC
DWSKTLKELDLNLSFDLHIEPISKFKLSESSMQHLRAGDIVALDDQYTFIQRIPRSHYVL
CHHHHHHHHCCCCEEEEEECCHHHHHCCHHHHHHHCCCCEEEECCCHHHHHHCCCCCEEE
SVGPVPYLYFLHEMRLLDVALMAFIAISLAFPVFIWMRPHWQDMLRIETAAQRFGEGHLS
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHH
ERIHFDSMSSFERLGVAFNQMADNINALIASKKQLIDGIAHELRTPLVRLRYRLEMSENL
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
SEAESQALNRDIGQLEALIEELLTYARLDRPQTELSLTTPDFPAWISDHVEDIQMVNPQR
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCH
EVALATLTHGNYGALDMRLMERVLDNLVNNALRYSSQRVAVSLTLQGSRASLLVEDDGPG
HEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCEEEEEECCCCC
IAPEERERVFEPFVRLDPSRDRATGGCGLGLAIVHSIAQAMGGEVRCEASPLGGARFCFS
CCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEE
WPVYHQLPDFTSA
CCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2646639; 1925016; 1495392 [H]