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The map label for this gene is malI [H]

Identifier: 238894528

GI number: 238894528

Start: 2424001

End: 2425029

Strand: Direct

Name: malI [H]

Synonym: KP1_2522

Alternate gene names: 238894528

Gene position: 2424001-2425029 (Clockwise)

Preceding gene: 238894525

Following gene: 238894531

Centisome position: 46.18

GC content: 64.82

Gene sequence:

>1029_bases
ATGGCTATCGCAAAAAAGATCACCATCAATGATGTGGCGCTGGCTGCCGGCGTCTCCGTGAGTACCGTTTCACTGGTACT
GAGCGGCAAAGGGCGCATCTCCAGCGCCACCGGCGAGCGGGTTAATCAGGCTATCGAACAGCTGGGATTTGTGCGCAATC
GTCAGGCGGCCTCGCTGCGCGGTGGGCAGAGCGGGGTGATTGGCCTTATCGTCAGCGACCTGTCAAAACCGTTTTATGCT
GAATTAACCGCCGGCCTCACCGATGCCCTGGAGAGGCAGGGGAAAATGGTATTTCTCACCCAGGGAGGCCGCAGCGGCGA
GAAGATGGCCCAGCGGTTTGATACGCTGGCGGCGCAGGGGGTGGACGGGGTGATTATCGCCGGGGCGATCGACCGCGGGA
GCGAGCTGCGCGAGCGTGCGGCGGAGGCGGGGGTGCCGCTGGTGTTTGCTTCCCGCGCCAGCTATCTTGACGATGTCGAC
CTGATCCGCCCGGACAACATGCAGGCGGCGCAGCTGGTGACGGAGCATCTTATCCGCCGCGGCCATCAGCGGATCGCCTG
GCTCGGCGGGCAGAGTGCTTCGCTGACGCGCGCCGAGCGGGTCGGCGGCTATTGCGCCACCTTGCTGAAATACGGCCTGC
CGTTTCACAGCGAATGGGTGCTGGAGTGCGAGTCCAGCCAGAAGCAGGCGGCGGAGGCGATGACCGGCCTGCTGCGCCAG
AATCCGACCATCACCGCGGTGCTGTGCTACAACAACGTGGTAGCCACCGGCGCGTGGTTCGGTCTGTTACGGGCTGGAAG
ACAGAGCGGGGAAGATGGCGTCGAGAGCTACTTTGAGCAGCGGGTGGCGCTGGCGGCGTTTGCCGACGTGCCGGAGGCCG
CCCTCGACGACCTGCCTCTCACCTGGGTGACCACCCCGGCGCGGGAGATGGGGAAAAGCCTGGCGGATTCGATGCTGCGG
CGGCTGGAAGAGGGGACTGGCGAGACGCGAAACCAGATTATGCCCCCGCGGCTGGTGACGCGAAAATGA

Upstream 100 bases:

>100_bases
AGTGCGATAAAAATCACATATTCAAGCGATAATAAGGTTTATAAAGATCGCACAATGATAAAACGATTTATCGACAGGCA
AGGGAGTCAGCCAGCAGGTT

Downstream 100 bases:

>100_bases
GGGAGACGGCGCGCGCTGGCCGTCTCCTGTACCGATAAGTTACGGCTGCGGGGCGGCGTCGTCCTGCTGAGGAGCGGCGT
CATCCTGCTGCGGCTGGCCT

Product: DNA-binding transcriptional repressor MalI

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MAIAKKITINDVALAAGVSVSTVSLVLSGKGRISSATGERVNQAIEQLGFVRNRQAASLRGGQSGVIGLIVSDLSKPFYA
ELTAGLTDALERQGKMVFLTQGGRSGEKMAQRFDTLAAQGVDGVIIAGAIDRGSELRERAAEAGVPLVFASRASYLDDVD
LIRPDNMQAAQLVTEHLIRRGHQRIAWLGGQSASLTRAERVGGYCATLLKYGLPFHSEWVLECESSQKQAAEAMTGLLRQ
NPTITAVLCYNNVVATGAWFGLLRAGRQSGEDGVESYFEQRVALAAFADVPEAALDDLPLTWVTTPAREMGKSLADSMLR
RLEEGTGETRNQIMPPRLVTRK

Sequences:

>Translated_342_residues
MAIAKKITINDVALAAGVSVSTVSLVLSGKGRISSATGERVNQAIEQLGFVRNRQAASLRGGQSGVIGLIVSDLSKPFYA
ELTAGLTDALERQGKMVFLTQGGRSGEKMAQRFDTLAAQGVDGVIIAGAIDRGSELRERAAEAGVPLVFASRASYLDDVD
LIRPDNMQAAQLVTEHLIRRGHQRIAWLGGQSASLTRAERVGGYCATLLKYGLPFHSEWVLECESSQKQAAEAMTGLLRQ
NPTITAVLCYNNVVATGAWFGLLRAGRQSGEDGVESYFEQRVALAAFADVPEAALDDLPLTWVTTPAREMGKSLADSMLR
RLEEGTGETRNQIMPPRLVTRK
>Mature_341_residues
AIAKKITINDVALAAGVSVSTVSLVLSGKGRISSATGERVNQAIEQLGFVRNRQAASLRGGQSGVIGLIVSDLSKPFYAE
LTAGLTDALERQGKMVFLTQGGRSGEKMAQRFDTLAAQGVDGVIIAGAIDRGSELRERAAEAGVPLVFASRASYLDDVDL
IRPDNMQAAQLVTEHLIRRGHQRIAWLGGQSASLTRAERVGGYCATLLKYGLPFHSEWVLECESSQKQAAEAMTGLLRQN
PTITAVLCYNNVVATGAWFGLLRAGRQSGEDGVESYFEQRVALAAFADVPEAALDDLPLTWVTTPAREMGKSLADSMLRR
LEEGTGETRNQIMPPRLVTRK

Specific function: Repressor for the malX and malY genes. Also regulates its own expression. Binds maltose as an inducer [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787906, Length=342, Percent_Identity=73.3918128654971, Blast_Score=529, Evalue=1e-151,
Organism=Escherichia coli, GI1790194, Length=334, Percent_Identity=27.2455089820359, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1790369, Length=322, Percent_Identity=25.1552795031056, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1786540, Length=341, Percent_Identity=28.7390029325513, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1788474, Length=344, Percent_Identity=28.1976744186047, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI48994940, Length=320, Percent_Identity=28.125, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1789202, Length=290, Percent_Identity=28.6206896551724, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1789068, Length=337, Percent_Identity=26.7062314540059, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1787948, Length=336, Percent_Identity=23.2142857142857, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1787580, Length=341, Percent_Identity=25.2199413489736, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790715, Length=330, Percent_Identity=24.2424242424242, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1790689, Length=271, Percent_Identity=25.0922509225092, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1786268, Length=187, Percent_Identity=23.5294117647059, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36732; Mature: 36601

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIAKKITINDVALAAGVSVSTVSLVLSGKGRISSATGERVNQAIEQLGFVRNRQAASLR
CCCCCEEEECHHHHHCCCCHHEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHCC
GGQSGVIGLIVSDLSKPFYAELTAGLTDALERQGKMVFLTQGGRSGEKMAQRFDTLAAQG
CCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCC
VDGVIIAGAIDRGSELRERAAEAGVPLVFASRASYLDDVDLIRPDNMQAAQLVTEHLIRR
CCCEEEEECCCCCHHHHHHHHHCCCCEEEECCHHHCCCCCCCCCCCCHHHHHHHHHHHHC
GHQRIAWLGGQSASLTRAERVGGYCATLLKYGLPFHSEWVLECESSQKQAAEAMTGLLRQ
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHEECCCHHHHHHHHHHHHHHC
NPTITAVLCYNNVVATGAWFGLLRAGRQSGEDGVESYFEQRVALAAFADVPEAALDDLPL
CCCEEEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCE
TWVTTPAREMGKSLADSMLRRLEEGTGETRNQIMPPRLVTRK
EEEECCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCC
>Mature Secondary Structure 
AIAKKITINDVALAAGVSVSTVSLVLSGKGRISSATGERVNQAIEQLGFVRNRQAASLR
CCCCEEEECHHHHHCCCCHHEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHCC
GGQSGVIGLIVSDLSKPFYAELTAGLTDALERQGKMVFLTQGGRSGEKMAQRFDTLAAQG
CCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCC
VDGVIIAGAIDRGSELRERAAEAGVPLVFASRASYLDDVDLIRPDNMQAAQLVTEHLIRR
CCCEEEEECCCCCHHHHHHHHHCCCCEEEECCHHHCCCCCCCCCCCCHHHHHHHHHHHHC
GHQRIAWLGGQSASLTRAERVGGYCATLLKYGLPFHSEWVLECESSQKQAAEAMTGLLRQ
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHEECCCHHHHHHHHHHHHHHC
NPTITAVLCYNNVVATGAWFGLLRAGRQSGEDGVESYFEQRVALAAFADVPEAALDDLPL
CCCEEEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCE
TWVTTPAREMGKSLADSMLRRLEEGTGETRNQIMPPRLVTRK
EEEECCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670898; 1856179; 9097039; 9278503 [H]