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The map label for this gene is fumA [H]

Identifier: 238894531

GI number: 238894531

Start: 2428095

End: 2429741

Strand: Direct

Name: fumA [H]

Synonym: KP1_2525

Alternate gene names: 238894531

Gene position: 2428095-2429741 (Clockwise)

Preceding gene: 238894528

Following gene: 238894532

Centisome position: 46.26

GC content: 59.2

Gene sequence:

>1647_bases
ATGTCGAATAAACCGTTTGTTTACCAGGATCCTTTTCCGCTCAAAAAGGATGACACCGAGTATTATCTCCTCAGCAGTGA
TTACGTCTCCGTCGCCGAATTCGCCGGCCAGGAAGTGCTAAAAGTTGACCCTCAGGCGCTGACCCTTCTGGCGCAGCATG
CTTTCCACGACGCCTCCTTTATGCTTCGCCCTGCGCATCAGCAGCAGGTGGCGGATATCCTTAACGATCCGGAAGCCAGC
GAAAACGATAAATACGTCGCGCTGCAGTTCCTGCGCAACTCGGATATCGCCGCCAAAGGTATTCTGCCCACCTGCCAGGA
CACCGGCACCGCGATCATCATGGGTAAAAAAGGCCAGCGCGTGTGGACCGGCGGCGGCGATGAAGCCGCCCTGGCGCAGG
GGGTGTACAACACCTATATCCAGGACAACCTGCGCTACTCGCAGAACGCGCCGCTGGATATGTATAAAGAGGTTAACACT
GGCACCAACCTGCCGGCGCAGATCGATCTCTATGCTACCGATGGCGATGAGTACAAATTCCTCTGCATCGCCAAGGGCGG
CGGCTCGGCGAACAAAACCTATCTGTACCAGGAAACCAAAGCGCTGATCACCCCGGCGAAGCTGAAAAATTACCTCGTCG
AGAAGATGCGCACCCTCGGTACTGCCGCCTGTCCACCGTACCATATCGCCTTCGTGATCGGCGGTACCTCGGCGGAAGCG
ACGCTGAAGACCGTGAAGCTGGCGTCCACCAAATACTACGATGGCCTGCCGACCGAAGGTAACGAGCACGGCCAGGCGTT
CCGCGACGTGCAGCTGGAGCAGGAGCTGCTGCTGGAGGCGCAGAACCTTGGTCTCGGCGCGCAGTTCGGCGGGAAATACT
TTGCCCACGATATCCGCGTGATCCGTCTGCCGCGTCACGGCGCTTCCTGCCCGGTAGGGATGGGGGTTTCCTGTTCCGCC
GACCGCAACATCAAAGCCAAAATTAACCGCGACGGCATCTGGATCGAGAAGCTGGAGAGCAACCCGGGGAAATATATCCC
TGAGCATCTGCGCCAGGCCGGGGAAGGCGAAGCGGTGAAGGTAAATCTTAACCAGCCGATGAGCGAGATCCTGGCGCAGC
TGTCGCAGTATCCGGTGTCCACCCGCCTGTCGCTGAGCGGGACTATCATCGTCGCCCGCGATATCGCCCACGCTAAGCTG
AAGGAGCTTATCGACAACGGCGAGGCGCTGCCGCAGTACGTCAAAGATCACCCGATCTACTACGCAGGTCCGGCCAAGAC
GCCGGACGGTTACGCCTCCGGCTCTTTGGGGCCGACTACCGCAGGCCGCATGGACTCTTACGTTGATCTGCTGCAGTCCC
ATGGGGCGAGCAAGATCATGCTGGCGAAAGGCAACCGCAGCCAGCAGGTCACCGACGCCTGCCACAAACACGGCGGCTTC
TACCTCGGCAGCATCGGCGGCCCGGCGGCAGTGCTGGCCCAGCAGAGCATCAAGAGCCTCGAGTGCGTGGCCTATCCGGA
GCTGGGTATGGAGGCTATCTGGAAAATTGAAGTGGAGGATTTCCCGGCCTTTATCCTCGTTGATGATAAAGGCAATGACT
TCTTCCAGCAGATCCAGACCTCGCAGTGCGCCCGCTGCGTGAAGTAA

Upstream 100 bases:

>100_bases
TTTGTTAAAAAATTGTGTAAGATTGTTCGACTCGCTTTTCTACAGGCACATGGAGTCCCTGCCTGAAACATAATCAGCCA
AAGCAGTAAGTGAGAGTACA

Downstream 100 bases:

>100_bases
CCCAGGCCGCCCTTCGGGGCGGCGCTGGTCGATGACAAACCTGATTTCGTTAGGGTGAGCGCCTTTCTGCCGGGTAGCGG
CTTTCGCCTTACCCGGCCTA

Product: aerobic class I fumarate hydratase/fumarase A

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MSNKPFVYQDPFPLKKDDTEYYLLSSDYVSVAEFAGQEVLKVDPQALTLLAQHAFHDASFMLRPAHQQQVADILNDPEAS
ENDKYVALQFLRNSDIAAKGILPTCQDTGTAIIMGKKGQRVWTGGGDEAALAQGVYNTYIQDNLRYSQNAPLDMYKEVNT
GTNLPAQIDLYATDGDEYKFLCIAKGGGSANKTYLYQETKALITPAKLKNYLVEKMRTLGTAACPPYHIAFVIGGTSAEA
TLKTVKLASTKYYDGLPTEGNEHGQAFRDVQLEQELLLEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSA
DRNIKAKINRDGIWIEKLESNPGKYIPEHLRQAGEGEAVKVNLNQPMSEILAQLSQYPVSTRLSLSGTIIVARDIAHAKL
KELIDNGEALPQYVKDHPIYYAGPAKTPDGYASGSLGPTTAGRMDSYVDLLQSHGASKIMLAKGNRSQQVTDACHKHGGF
YLGSIGGPAAVLAQQSIKSLECVAYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQTSQCARCVK

Sequences:

>Translated_548_residues
MSNKPFVYQDPFPLKKDDTEYYLLSSDYVSVAEFAGQEVLKVDPQALTLLAQHAFHDASFMLRPAHQQQVADILNDPEAS
ENDKYVALQFLRNSDIAAKGILPTCQDTGTAIIMGKKGQRVWTGGGDEAALAQGVYNTYIQDNLRYSQNAPLDMYKEVNT
GTNLPAQIDLYATDGDEYKFLCIAKGGGSANKTYLYQETKALITPAKLKNYLVEKMRTLGTAACPPYHIAFVIGGTSAEA
TLKTVKLASTKYYDGLPTEGNEHGQAFRDVQLEQELLLEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSA
DRNIKAKINRDGIWIEKLESNPGKYIPEHLRQAGEGEAVKVNLNQPMSEILAQLSQYPVSTRLSLSGTIIVARDIAHAKL
KELIDNGEALPQYVKDHPIYYAGPAKTPDGYASGSLGPTTAGRMDSYVDLLQSHGASKIMLAKGNRSQQVTDACHKHGGF
YLGSIGGPAAVLAQQSIKSLECVAYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQTSQCARCVK
>Mature_547_residues
SNKPFVYQDPFPLKKDDTEYYLLSSDYVSVAEFAGQEVLKVDPQALTLLAQHAFHDASFMLRPAHQQQVADILNDPEASE
NDKYVALQFLRNSDIAAKGILPTCQDTGTAIIMGKKGQRVWTGGGDEAALAQGVYNTYIQDNLRYSQNAPLDMYKEVNTG
TNLPAQIDLYATDGDEYKFLCIAKGGGSANKTYLYQETKALITPAKLKNYLVEKMRTLGTAACPPYHIAFVIGGTSAEAT
LKTVKLASTKYYDGLPTEGNEHGQAFRDVQLEQELLLEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSAD
RNIKAKINRDGIWIEKLESNPGKYIPEHLRQAGEGEAVKVNLNQPMSEILAQLSQYPVSTRLSLSGTIIVARDIAHAKLK
ELIDNGEALPQYVKDHPIYYAGPAKTPDGYASGSLGPTTAGRMDSYVDLLQSHGASKIMLAKGNRSQQVTDACHKHGGFY
LGSIGGPAAVLAQQSIKSLECVAYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQTSQCARCVK

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1787897, Length=548, Percent_Identity=90.3284671532847, Blast_Score=1040, Evalue=0.0,
Organism=Escherichia coli, GI1790564, Length=548, Percent_Identity=88.6861313868613, Blast_Score=1023, Evalue=0.0,
Organism=Escherichia coli, GI1789443, Length=168, Percent_Identity=29.7619047619048, Blast_Score=79, Evalue=5e-16,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 59890; Mature: 59758

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKPFVYQDPFPLKKDDTEYYLLSSDYVSVAEFAGQEVLKVDPQALTLLAQHAFHDASF
CCCCCEEECCCCCCCCCCCCEEEEECCCHHHHHHCCCCEEEECHHHHHHHHHHHHCCCCE
MLRPAHQQQVADILNDPEASENDKYVALQFLRNSDIAAKGILPTCQDTGTAIIMGKKGQR
EECCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCE
VWTGGGDEAALAQGVYNTYIQDNLRYSQNAPLDMYKEVNTGTNLPAQIDLYATDGDEYKF
EEECCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCEEEEEEEECCCCEEE
LCIAKGGGSANKTYLYQETKALITPAKLKNYLVEKMRTLGTAACPPYHIAFVIGGTSAEA
EEEECCCCCCCCEEEEECHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCH
TLKTVKLASTKYYDGLPTEGNEHGQAFRDVQLEQELLLEAQNLGLGAQFGGKYFAHDIRV
HEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECEEE
IRLPRHGASCPVGMGVSCSADRNIKAKINRDGIWIEKLESNPGKYIPEHLRQAGEGEAVK
EEECCCCCCCCCCCCEECCCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHCCCCCEEE
VNLNQPMSEILAQLSQYPVSTRLSLSGTIIVARDIAHAKLKELIDNGEALPQYVKDHPIY
EECCCCHHHHHHHHHHCCCCEEEEECEEEEEEECHHHHHHHHHHCCCHHHHHHHHHCCEE
YAGPAKTPDGYASGSLGPTTAGRMDSYVDLLQSHGASKIMLAKGNRSQQVTDACHKHGGF
EECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCE
YLGSIGGPAAVLAQQSIKSLECVAYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQT
EEECCCCHHHHHHHHHHHHEEEEECCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHHHH
SQCARCVK
HHHHHHCC
>Mature Secondary Structure 
SNKPFVYQDPFPLKKDDTEYYLLSSDYVSVAEFAGQEVLKVDPQALTLLAQHAFHDASF
CCCCEEECCCCCCCCCCCCEEEEECCCHHHHHHCCCCEEEECHHHHHHHHHHHHCCCCE
MLRPAHQQQVADILNDPEASENDKYVALQFLRNSDIAAKGILPTCQDTGTAIIMGKKGQR
EECCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCE
VWTGGGDEAALAQGVYNTYIQDNLRYSQNAPLDMYKEVNTGTNLPAQIDLYATDGDEYKF
EEECCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCEEEEEEEECCCCEEE
LCIAKGGGSANKTYLYQETKALITPAKLKNYLVEKMRTLGTAACPPYHIAFVIGGTSAEA
EEEECCCCCCCCEEEEECHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCH
TLKTVKLASTKYYDGLPTEGNEHGQAFRDVQLEQELLLEAQNLGLGAQFGGKYFAHDIRV
HEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECEEE
IRLPRHGASCPVGMGVSCSADRNIKAKINRDGIWIEKLESNPGKYIPEHLRQAGEGEAVK
EEECCCCCCCCCCCCEECCCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHCCCCCEEE
VNLNQPMSEILAQLSQYPVSTRLSLSGTIIVARDIAHAKLKELIDNGEALPQYVKDHPIY
EECCCCHHHHHHHHHHCCCCEEEEECEEEEEEECHHHHHHHHHHCCCHHHHHHHHHCCEE
YAGPAKTPDGYASGSLGPTTAGRMDSYVDLLQSHGASKIMLAKGNRSQQVTDACHKHGGF
EECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCE
YLGSIGGPAAVLAQQSIKSLECVAYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQT
EEECCCCHHHHHHHHHHHHEEEEECCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHHHH
SQCARCVK
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]