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The map label for this gene is gdhA [H]

Identifier: 238894504

GI number: 238894504

Start: 2400942

End: 2402216

Strand: Reverse

Name: gdhA [H]

Synonym: KP1_2495

Alternate gene names: 238894504

Gene position: 2402216-2400942 (Counterclockwise)

Preceding gene: 238894505

Following gene: 238894502

Centisome position: 45.77

GC content: 60.24

Gene sequence:

>1275_bases
ATGGAAAAGTTATCTTACGCATCTGAAAGTAGTACATCTCCATGGACCACCTACCTGCGCCAGATCGACCGCGTCGCGCC
TTATCTCGGCGATCTTGCCTACTGGGTGGAAACCCTGCGCCATCCGAAGCGCGCGCTGATCGTCGACATTCCTGTGCAAA
TGGACGACGGCACCATCCGCCACTTCGAGGGCTATCGCGTTCAGCACAATCTGTCGCGCGGACCGGGTAAAGGTGGTGTT
CGCTACCATCCGGATGTCGATCTTAACGAAGTCATGGCACTTTCTGCCTGGATGACCATTAAGTGCGCCGCGGTCAATAT
TCCTTACGGCGGGGCCAAAGGCGGCATCCGCGTCGACCCCTTCTCCTTGTCGGAAGGTGAACTGGAGCGTCTGACCCGCC
GCTACACCAGCGAAATCGGCATTATTATCGGGCCGCAGAAAGATATTCCGGCGCCGGACGTCGGCACCAATGGCAAAGTG
ATGGCATGGATGATGGACACCTACTCCATGAACCATGGCACCACCATCACTGGCGTGGTCACCGGCAAACCGATCCACCT
CGGCGGCTCCCTCGGTCGTGAAAAAGCCACCGGACGCGGCGTGTTTGTCACCGGCCGGGAAGTTGCCCGTCGCGCCGGTA
TCGAAATTGAAGGCGCCAAAGTGGCGCTGCAGGGCTTTGGTAACGTCGGTAGCGAAGCGGCCCGCCTTTTCGCCGGCGTC
GGCGCTCGTATCGTGGTTATTCAGGATCATACCGCGACCCTGTATAACGAAGGCGGAATCGATATGGCCGCCCTCACCGC
CTGGCAGGCGGAGAAAAAGCAGATTGCCGGTTTCCCGGGCGCCCAGGAGATCGATAAAGACGCCTTCTGGACCACCCCGA
TGGACATCCTGATCCCGGCGGCGCTGGAAGGGCAAATCACCCGCGAGCGCGCTGAGAAGCTGACCTGCAAACTGGTGCTG
GAAGGGGCCAACGGCCCAACCTATCCGGAAGCGGACGACGTGCTGGCCGAGCGCGGCGTGATCGTGGTGCCGGACGTTAT
CTGCAACGCCGGCGGGGTGACCGTCAGCTACTTTGAGTGGGTACAGGACATGGCCAGCTTCTTCTGGAGCGAAGAGGAGA
TCAACGCCAAAATGGACCGCATTATGACCGACGCTATCGTGCACGTCTGGGATAAAGCCGCCGAGAAAGAGTGTACGCTG
CGCACCGCCGCCTATATCGTGGCCTGCGAGCGCATCCTGATGGCGCGTAAAGACCGCGGCATCTACCCGGGCTGA

Upstream 100 bases:

>100_bases
GCTTTATAAAATTGGTGATACCATTCATTGCACAGAGTTTACTGCTGTAGCTATTCCACGGTTTTACACGACATAAATAA
CACTGAGAAACAGGACTCTT

Downstream 100 bases:

>100_bases
TCCCCATCAGCAACCGCATCAGGGGTGGCCTTGCGCCGCCCCTTTTTTTCTTTGCCGCGCCGTTAACCGCCGAGGATGTG
CGCTATCTGACGAGGAGTAG

Product: putative glutamic dehyrogenase-like protein

Products: NA

Alternate protein names: GDH [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MEKLSYASESSTSPWTTYLRQIDRVAPYLGDLAYWVETLRHPKRALIVDIPVQMDDGTIRHFEGYRVQHNLSRGPGKGGV
RYHPDVDLNEVMALSAWMTIKCAAVNIPYGGAKGGIRVDPFSLSEGELERLTRRYTSEIGIIIGPQKDIPAPDVGTNGKV
MAWMMDTYSMNHGTTITGVVTGKPIHLGGSLGREKATGRGVFVTGREVARRAGIEIEGAKVALQGFGNVGSEAARLFAGV
GARIVVIQDHTATLYNEGGIDMAALTAWQAEKKQIAGFPGAQEIDKDAFWTTPMDILIPAALEGQITRERAEKLTCKLVL
EGANGPTYPEADDVLAERGVIVVPDVICNAGGVTVSYFEWVQDMASFFWSEEEINAKMDRIMTDAIVHVWDKAAEKECTL
RTAAYIVACERILMARKDRGIYPG

Sequences:

>Translated_424_residues
MEKLSYASESSTSPWTTYLRQIDRVAPYLGDLAYWVETLRHPKRALIVDIPVQMDDGTIRHFEGYRVQHNLSRGPGKGGV
RYHPDVDLNEVMALSAWMTIKCAAVNIPYGGAKGGIRVDPFSLSEGELERLTRRYTSEIGIIIGPQKDIPAPDVGTNGKV
MAWMMDTYSMNHGTTITGVVTGKPIHLGGSLGREKATGRGVFVTGREVARRAGIEIEGAKVALQGFGNVGSEAARLFAGV
GARIVVIQDHTATLYNEGGIDMAALTAWQAEKKQIAGFPGAQEIDKDAFWTTPMDILIPAALEGQITRERAEKLTCKLVL
EGANGPTYPEADDVLAERGVIVVPDVICNAGGVTVSYFEWVQDMASFFWSEEEINAKMDRIMTDAIVHVWDKAAEKECTL
RTAAYIVACERILMARKDRGIYPG
>Mature_424_residues
MEKLSYASESSTSPWTTYLRQIDRVAPYLGDLAYWVETLRHPKRALIVDIPVQMDDGTIRHFEGYRVQHNLSRGPGKGGV
RYHPDVDLNEVMALSAWMTIKCAAVNIPYGGAKGGIRVDPFSLSEGELERLTRRYTSEIGIIIGPQKDIPAPDVGTNGKV
MAWMMDTYSMNHGTTITGVVTGKPIHLGGSLGREKATGRGVFVTGREVARRAGIEIEGAKVALQGFGNVGSEAARLFAGV
GARIVVIQDHTATLYNEGGIDMAALTAWQAEKKQIAGFPGAQEIDKDAFWTTPMDILIPAALEGQITRERAEKLTCKLVL
EGANGPTYPEADDVLAERGVIVVPDVICNAGGVTVSYFEWVQDMASFFWSEEEINAKMDRIMTDAIVHVWDKAAEKECTL
RTAAYIVACERILMARKDRGIYPG

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=332, Percent_Identity=42.4698795180723, Blast_Score=270, Evalue=2e-72,
Organism=Homo sapiens, GI4885281, Length=332, Percent_Identity=42.1686746987952, Blast_Score=266, Evalue=2e-71,
Organism=Escherichia coli, GI1788059, Length=362, Percent_Identity=33.4254143646409, Blast_Score=182, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17544676, Length=377, Percent_Identity=37.9310344827586, Blast_Score=260, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6324951, Length=381, Percent_Identity=28.8713910761155, Blast_Score=136, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6319256, Length=386, Percent_Identity=29.2746113989637, Blast_Score=136, Evalue=6e-33,
Organism=Drosophila melanogaster, GI24649283, Length=330, Percent_Identity=44.2424242424242, Blast_Score=275, Evalue=5e-74,
Organism=Drosophila melanogaster, GI45553475, Length=330, Percent_Identity=42.1212121212121, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI45549226, Length=330, Percent_Identity=42.1212121212121, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24649460, Length=280, Percent_Identity=42.1428571428571, Blast_Score=223, Evalue=2e-58,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 46380; Mature: 46380

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS00074 GLFV_DEHYDROGENASE ; PS00137 SUBTILASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLSYASESSTSPWTTYLRQIDRVAPYLGDLAYWVETLRHPKRALIVDIPVQMDDGTIR
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCEE
HFEGYRVQHNLSRGPGKGGVRYHPDVDLNEVMALSAWMTIKCAAVNIPYGGAKGGIRVDP
ECCCEEEEECCCCCCCCCCCEECCCCCHHHHHHHHHCEEEEEEEEECCCCCCCCCEEECC
FSLSEGELERLTRRYTSEIGIIIGPQKDIPAPDVGTNGKVMAWMMDTYSMNHGTTITGVV
CCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEEE
TGKPIHLGGSLGREKATGRGVFVTGREVARRAGIEIEGAKVALQGFGNVGSEAARLFAGV
ECCEEEECCCCCCCCCCCCEEEEECHHHHHHCCCEECCCEEEEECCCCCCHHHHHHHHCC
GARIVVIQDHTATLYNEGGIDMAALTAWQAEKKQIAGFPGAQEIDKDAFWTTPMDILIPA
CEEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCHHHEECC
ALEGQITRERAEKLTCKLVLEGANGPTYPEADDVLAERGVIVVPDVICNAGGVTVSYFEW
HHCCHHHHHHHHCEEEEEEEECCCCCCCCCHHHHHHHCCEEEECHHHCCCCCCCHHHHHH
VQDMASFFWSEEEINAKMDRIMTDAIVHVWDKAAEKECTLRTAAYIVACERILMARKDRG
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC
IYPG
CCCC
>Mature Secondary Structure
MEKLSYASESSTSPWTTYLRQIDRVAPYLGDLAYWVETLRHPKRALIVDIPVQMDDGTIR
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCEE
HFEGYRVQHNLSRGPGKGGVRYHPDVDLNEVMALSAWMTIKCAAVNIPYGGAKGGIRVDP
ECCCEEEEECCCCCCCCCCCEECCCCCHHHHHHHHHCEEEEEEEEECCCCCCCCCEEECC
FSLSEGELERLTRRYTSEIGIIIGPQKDIPAPDVGTNGKVMAWMMDTYSMNHGTTITGVV
CCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEEE
TGKPIHLGGSLGREKATGRGVFVTGREVARRAGIEIEGAKVALQGFGNVGSEAARLFAGV
ECCEEEECCCCCCCCCCCCEEEEECHHHHHHCCCEECCCEEEEECCCCCCHHHHHHHHCC
GARIVVIQDHTATLYNEGGIDMAALTAWQAEKKQIAGFPGAQEIDKDAFWTTPMDILIPA
CEEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCHHHEECC
ALEGQITRERAEKLTCKLVLEGANGPTYPEADDVLAERGVIVVPDVICNAGGVTVSYFEW
HHCCHHHHHHHHCEEEEEEEECCCCCCCCCHHHHHHHCCEEEECHHHCCCCCCCHHHHHH
VQDMASFFWSEEEINAKMDRIMTDAIVHVWDKAAEKECTLRTAAYIVACERILMARKDRG
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC
IYPG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9680336; 10360571; 9135121; 9654452; 10366510 [H]