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The map label for this gene is pspF [H]

Identifier: 238894359

GI number: 238894359

Start: 2252301

End: 2253290

Strand: Reverse

Name: pspF [H]

Synonym: KP1_2341

Alternate gene names: 238894359

Gene position: 2253290-2252301 (Counterclockwise)

Preceding gene: 238894366

Following gene: 238894356

Centisome position: 42.93

GC content: 62.32

Gene sequence:

>990_bases
ATGGCGAAATTCATCATGGCGCAGTACAAGGATAATCTGCTGGGCGAGGCCAACAGCTTTCTCGAGGTGCTGGAACAGGT
TTCCCGGCTGGCCCCGCTGGATAAACCGGTGCTGGTCATCGGCGAGCGCGGCACCGGGAAAGAGCTTATCGCTAACCGTC
TCCACTACCTCTCCAGCCGCTGGCAGGGGCCGTTTATCTCGTTGAACTGCGCGGCGCTGAACGATAATCTGCTCGACTCG
GAGCTGTTCGGCCACGAAGCCGGGGCTTTCACCGGGGCCAGCAAGCGCCATCCCGGGCGCTTCGAGCGCGCCGACGGCGG
GACGCTGTTTCTCGATGAGCTGGCGACAGCCCCAATGCTGGTGCAGGAGAAGCTGTTAAGAGTGATTGAATATGGCGAGC
TGGAGCGCGTGGGCGGCAGCCAGCCGCTGCAGGTTAACGTCCGGCTGGTGTGCGCCACCAACGCTGACCTGCCGCAGATG
GTCGAGGAAGGTCATTTTCGCGCCGACCTGCTCGACCGCCTGGCCTTCGACGTGGTACAGCTCCCCCCGCTGCGCGACCG
GCAGAGCGACATCATGTTGCTCGCCAACCAGTTTGCCATCCAGATGTGCCGCGAACTCGGTCTGCCGCTGTTTCCCGGCT
TTAGCGAACGAGCGACCGCGACGCTGCTCGGCTACCGCTGGCCGGGGAATATTCGCGAACTGAAGAACGTGGTTGAGCGC
TCGGTGTATCGCCACGGCGACAGCGAACATGAGCTGGACGCGATTATTATTAACCCTTTTCGTCAGTCCCCCGGCTCTCC
GCCGGAAGCCGCGCCCGGCGATGAGCTGCCCGCGCTACCGCTGGATCTGCGCGATTTCCAGTTCCAGCAGGAAAAACGCC
TGCTGCAGCGGAGTCTGGAGCAGGCGAAGTATCATCAGAAGCAGGCGGCGGAGCTGCTGGGATTAACCTATCACCAGCTC
CGCGCGCTGCTGAAAAAGCATCAGCTGTGA

Upstream 100 bases:

>100_bases
TACAAATGGCGTGCCAACTTTTTATCTTATTGATTTTAAACAGGGTGGTTGCGAAACGTGCTGGGCATTGCTACAGTCAC
CTGGTTAATTACACTACTTA

Downstream 100 bases:

>100_bases
GAGGCGCGCGGCAGCAGCAGCGCACTCACCGCCATCGCCCCCAGACATAAGACGGCGTTAAACCACAGGGCATACTGGGC
GGCATGGGCCATGCCCAGCG

Product: phage shock protein operon transcriptional activator

Products: NA

Alternate protein names: Phage shock protein F [H]

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MAKFIMAQYKDNLLGEANSFLEVLEQVSRLAPLDKPVLVIGERGTGKELIANRLHYLSSRWQGPFISLNCAALNDNLLDS
ELFGHEAGAFTGASKRHPGRFERADGGTLFLDELATAPMLVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPQM
VEEGHFRADLLDRLAFDVVQLPPLRDRQSDIMLLANQFAIQMCRELGLPLFPGFSERATATLLGYRWPGNIRELKNVVER
SVYRHGDSEHELDAIIINPFRQSPGSPPEAAPGDELPALPLDLRDFQFQQEKRLLQRSLEQAKYHQKQAAELLGLTYHQL
RALLKKHQL

Sequences:

>Translated_329_residues
MAKFIMAQYKDNLLGEANSFLEVLEQVSRLAPLDKPVLVIGERGTGKELIANRLHYLSSRWQGPFISLNCAALNDNLLDS
ELFGHEAGAFTGASKRHPGRFERADGGTLFLDELATAPMLVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPQM
VEEGHFRADLLDRLAFDVVQLPPLRDRQSDIMLLANQFAIQMCRELGLPLFPGFSERATATLLGYRWPGNIRELKNVVER
SVYRHGDSEHELDAIIINPFRQSPGSPPEAAPGDELPALPLDLRDFQFQQEKRLLQRSLEQAKYHQKQAAELLGLTYHQL
RALLKKHQL
>Mature_328_residues
AKFIMAQYKDNLLGEANSFLEVLEQVSRLAPLDKPVLVIGERGTGKELIANRLHYLSSRWQGPFISLNCAALNDNLLDSE
LFGHEAGAFTGASKRHPGRFERADGGTLFLDELATAPMLVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPQMV
EEGHFRADLLDRLAFDVVQLPPLRDRQSDIMLLANQFAIQMCRELGLPLFPGFSERATATLLGYRWPGNIRELKNVVERS
VYRHGDSEHELDAIIINPFRQSPGSPPEAAPGDELPALPLDLRDFQFQQEKRLLQRSLEQAKYHQKQAAELLGLTYHQLR
ALLKKHQL

Specific function: Transcriptional activator for the phage shock protein (psp) operon (pspABCDE) and pspG gene [H]

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87081872, Length=327, Percent_Identity=84.0978593272171, Blast_Score=555, Evalue=1e-159,
Organism=Escherichia coli, GI1788550, Length=324, Percent_Identity=41.0493827160494, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI87082117, Length=231, Percent_Identity=49.7835497835498, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1790437, Length=317, Percent_Identity=41.6403785488959, Blast_Score=221, Evalue=7e-59,
Organism=Escherichia coli, GI1789233, Length=326, Percent_Identity=39.5705521472393, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1789087, Length=232, Percent_Identity=48.2758620689655, Blast_Score=211, Evalue=6e-56,
Organism=Escherichia coli, GI1790299, Length=331, Percent_Identity=39.5770392749245, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI87082152, Length=323, Percent_Identity=37.1517027863777, Blast_Score=194, Evalue=9e-51,
Organism=Escherichia coli, GI1788905, Length=322, Percent_Identity=36.9565217391304, Blast_Score=188, Evalue=5e-49,
Organism=Escherichia coli, GI1786524, Length=241, Percent_Identity=42.3236514522822, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI1787583, Length=220, Percent_Identity=40.4545454545455, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1789828, Length=232, Percent_Identity=34.9137931034483, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI87081858, Length=204, Percent_Identity=33.8235294117647, Blast_Score=108, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR014317 [H]

Pfam domain/function: PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 37050; Mature: 36919

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKFIMAQYKDNLLGEANSFLEVLEQVSRLAPLDKPVLVIGERGTGKELIANRLHYLSSR
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHC
WQGPFISLNCAALNDNLLDSELFGHEAGAFTGASKRHPGRFERADGGTLFLDELATAPML
CCCCEEEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHCCCHHH
VQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPQMVEEGHFRADLLDRLAFDVVQ
HHHHHHHHHHHCCHHHCCCCCCEEEEEEEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHC
LPPLRDRQSDIMLLANQFAIQMCRELGLPLFPGFSERATATLLGYRWPGNIRELKNVVER
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHEEECCCCCHHHHHHHHHH
SVYRHGDSEHELDAIIINPFRQSPGSPPEAAPGDELPALPLDLRDFQFQQEKRLLQRSLE
HHHHCCCCCCCCCEEEEECHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
QAKYHQKQAAELLGLTYHQLRALLKKHQL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKFIMAQYKDNLLGEANSFLEVLEQVSRLAPLDKPVLVIGERGTGKELIANRLHYLSSR
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHC
WQGPFISLNCAALNDNLLDSELFGHEAGAFTGASKRHPGRFERADGGTLFLDELATAPML
CCCCEEEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHCCCHHH
VQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPQMVEEGHFRADLLDRLAFDVVQ
HHHHHHHHHHHCCHHHCCCCCCEEEEEEEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHC
LPPLRDRQSDIMLLANQFAIQMCRELGLPLFPGFSERATATLLGYRWPGNIRELKNVVER
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHEEECCCCCHHHHHHHHHH
SVYRHGDSEHELDAIIINPFRQSPGSPPEAAPGDELPALPLDLRDFQFQQEKRLLQRSLE
HHHHCCCCCCCCCEEEEECHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
QAKYHQKQAAELLGLTYHQLRALLKKHQL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8606168; 9097039; 9278503; 1712397; 7984428; 7920643 [H]