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The map label for this gene is norB [H]

Identifier: 238894358

GI number: 238894358

Start: 2250934

End: 2252310

Strand: Direct

Name: norB [H]

Synonym: KP1_2340

Alternate gene names: 238894358

Gene position: 2250934-2252310 (Clockwise)

Preceding gene: 238894357

Following gene: 238894360

Centisome position: 42.89

GC content: 63.76

Gene sequence:

>1377_bases
ATAAGCGACACCCCGTTTGTCGGCAACGATCGCCTGCTGGTGGGGATTGTGTTAAGCGTTTTAACCTTCTGGCTGTTCGC
CCAGTCGGTGATTAACGTGGTGCCGGCGATGAAAAGCAGCCTTGATATCTCTCTTGAAACCTTAACCCTGGCCGTCAGTC
TCAGCGCGCTGTTCAGCGGCTGCTTCGTGGTCGCCAGCGGCGGCCTGGCGGACAAGTTTGGCCGGATGCGGATGACCACC
CTCGGGCTGGGGCTGAGCATCGTCGGCAGCGCCATGCTGGTGGTCGCTCAGGGACCGGGGCTGTTCCTCGCCGGCCGGGT
GCTGCAGGGGCTGTCGGCGGCCTGCATTATGCCGGCGACGCTGGCGCTGATTAAGACCTGGTATGAGGGGCGTGCGCGGC
AGCGGGCGGTCAGCTTCTGGGTCATCGGCTCGTGGGGCGGCAGCGGTCTCTGTTCCTTCGTTGGCGGGGCTATCGCCACC
GGACTGGGCTGGCGCTGGATCTTCGTCTTCTCGATCGCCGTTGCCCTGCTGGCGCTGTTTTTGCTGCGCGGCACGCCGGA
GAGCCGCAGCGCCAGCGCCAGCCAGCATAAGCTGGACGTCGGCGGTCTGCTGAGCCTGATTGTTGCGCTGGTGCTGGTGA
ATCTGTTTATCAGCAAGGGCCACGGCTGGGGCTGGAGCAGCCCGCTGTCGTTGACCATGCTGGCCGGGACGCTGGCGGCG
GGGACGATATTTATCCGCAACGGCATGCGCAAAGGCGAGGCGGCGCTGATTGATTTTGCGCTGTTTCGCAATCGGGCCTA
CGGTGCGGCAGTGCTGTCGAACTTTCTGCTCAATGGGGCGATTGGCACTATGATGATCGCCAGCATCTGGCTGCAGCAAG
GGCACCACCTGACGCCGCTGGAGAGCGGGATGATGACCCTCGGCTATCTGGTGACCGTCCTGGCGATGATCCGGGTCGGC
GAGAAGCTGCTGCAGCGCTATGGCGCCCGGCTGCCGATGATGGCCGGTCCGGTGTTGACCGCTATCGCCATCGCGCTGAT
ATCCTGCACCTTTCTCGAAAAGGCGCTGTATATCGGGGTGGTGTTCGCCAGCAACGTGCTGTTTGGCCTCGGGCTGGGCT
GCTACGCCACGCCATCGACGGATACCGCGGTGGCCAATGCCCCGGAGAACAAAATCGGCGTCGCCTCGGGGATCTATAAG
ATGGGCAGCTCGCTGGGCGGGGCGATGGGTATCGCCGTGACAGCGTCACTTTTCGCGCTGTTTTTACCGCTGGGCATGGC
CCATGCCGCCCAGTATGCCCTGTGGTTTAACGCCGTCTTATGTCTGGGGGCGATGGCGGTGAGTGCGCTGCTGCTGCCGC
GCGCCTCTCACAGCTGA

Upstream 100 bases:

>100_bases
GCGCTGTTGCTGACCGGCGCGGCGCTGTGGTGCGGCCTGACGGAACGCTATCTGCGCTAATCACGAGAAACGGGAGGGCC
ATGCGATGAGCACTGTCACA

Downstream 100 bases:

>100_bases
TGCTTTTTCAGCAGCGCGCGGAGCTGGTGATAGGTTAATCCCAGCAGCTCCGCCGCCTGCTTCTGATGATACTTCGCCTG
CTCCAGACTCCGCTGCAGCA

Product: putative transmembrane efflux protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MSDTPFVGNDRLLVGIVLSVLTFWLFAQSVINVVPAMKSSLDISLETLTLAVSLSALFSGCFVVASGGLADKFGRMRMTT
LGLGLSIVGSAMLVVAQGPGLFLAGRVLQGLSAACIMPATLALIKTWYEGRARQRAVSFWVIGSWGGSGLCSFVGGAIAT
GLGWRWIFVFSIAVALLALFLLRGTPESRSASASQHKLDVGGLLSLIVALVLVNLFISKGHGWGWSSPLSLTMLAGTLAA
GTIFIRNGMRKGEAALIDFALFRNRAYGAAVLSNFLLNGAIGTMMIASIWLQQGHHLTPLESGMMTLGYLVTVLAMIRVG
EKLLQRYGARLPMMAGPVLTAIAIALISCTFLEKALYIGVVFASNVLFGLGLGCYATPSTDTAVANAPENKIGVASGIYK
MGSSLGGAMGIAVTASLFALFLPLGMAHAAQYALWFNAVLCLGAMAVSALLLPRASHS

Sequences:

>Translated_458_residues
MSDTPFVGNDRLLVGIVLSVLTFWLFAQSVINVVPAMKSSLDISLETLTLAVSLSALFSGCFVVASGGLADKFGRMRMTT
LGLGLSIVGSAMLVVAQGPGLFLAGRVLQGLSAACIMPATLALIKTWYEGRARQRAVSFWVIGSWGGSGLCSFVGGAIAT
GLGWRWIFVFSIAVALLALFLLRGTPESRSASASQHKLDVGGLLSLIVALVLVNLFISKGHGWGWSSPLSLTMLAGTLAA
GTIFIRNGMRKGEAALIDFALFRNRAYGAAVLSNFLLNGAIGTMMIASIWLQQGHHLTPLESGMMTLGYLVTVLAMIRVG
EKLLQRYGARLPMMAGPVLTAIAIALISCTFLEKALYIGVVFASNVLFGLGLGCYATPSTDTAVANAPENKIGVASGIYK
MGSSLGGAMGIAVTASLFALFLPLGMAHAAQYALWFNAVLCLGAMAVSALLLPRASHS
>Mature_457_residues
SDTPFVGNDRLLVGIVLSVLTFWLFAQSVINVVPAMKSSLDISLETLTLAVSLSALFSGCFVVASGGLADKFGRMRMTTL
GLGLSIVGSAMLVVAQGPGLFLAGRVLQGLSAACIMPATLALIKTWYEGRARQRAVSFWVIGSWGGSGLCSFVGGAIATG
LGWRWIFVFSIAVALLALFLLRGTPESRSASASQHKLDVGGLLSLIVALVLVNLFISKGHGWGWSSPLSLTMLAGTLAAG
TIFIRNGMRKGEAALIDFALFRNRAYGAAVLSNFLLNGAIGTMMIASIWLQQGHHLTPLESGMMTLGYLVTVLAMIRVGE
KLLQRYGARLPMMAGPVLTAIAIALISCTFLEKALYIGVVFASNVLFGLGLGCYATPSTDTAVANAPENKIGVASGIYKM
GSSLGGAMGIAVTASLFALFLPLGMAHAAQYALWFNAVLCLGAMAVSALLLPRASHS

Specific function: Multidrug efflux pump that acts independently of norA and is one of the factors that confers resistance against diverse quinolones and chemical compounds [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Escherichia coli, GI1788710, Length=431, Percent_Identity=22.737819025522, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1790195, Length=395, Percent_Identity=23.5443037974684, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1789042, Length=427, Percent_Identity=20.8430913348946, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788392, Length=413, Percent_Identity=23.9709443099274, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI87081983, Length=361, Percent_Identity=24.0997229916898, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47864; Mature: 47732

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTPFVGNDRLLVGIVLSVLTFWLFAQSVINVVPAMKSSLDISLETLTLAVSLSALFSG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
CFVVASGGLADKFGRMRMTTLGLGLSIVGSAMLVVAQGPGLFLAGRVLQGLSAACIMPAT
HHHHCCCCHHHHHCCHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHCCHHHHHHHHH
LALIKTWYEGRARQRAVSFWVIGSWGGSGLCSFVGGAIATGLGWRWIFVFSIAVALLALF
HHHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LLRGTPESRSASASQHKLDVGGLLSLIVALVLVNLFISKGHGWGWSSPLSLTMLAGTLAA
HHCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
GTIFIRNGMRKGEAALIDFALFRNRAYGAAVLSNFLLNGAIGTMMIASIWLQQGHHLTPL
HHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
ESGMMTLGYLVTVLAMIRVGEKLLQRYGARLPMMAGPVLTAIAIALISCTFLEKALYIGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VFASNVLFGLGLGCYATPSTDTAVANAPENKIGVASGIYKMGSSLGGAMGIAVTASLFAL
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
FLPLGMAHAAQYALWFNAVLCLGAMAVSALLLPRASHS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SDTPFVGNDRLLVGIVLSVLTFWLFAQSVINVVPAMKSSLDISLETLTLAVSLSALFSG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
CFVVASGGLADKFGRMRMTTLGLGLSIVGSAMLVVAQGPGLFLAGRVLQGLSAACIMPAT
HHHHCCCCHHHHHCCHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHCCHHHHHHHHH
LALIKTWYEGRARQRAVSFWVIGSWGGSGLCSFVGGAIATGLGWRWIFVFSIAVALLALF
HHHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LLRGTPESRSASASQHKLDVGGLLSLIVALVLVNLFISKGHGWGWSSPLSLTMLAGTLAA
HHCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
GTIFIRNGMRKGEAALIDFALFRNRAYGAAVLSNFLLNGAIGTMMIASIWLQQGHHLTPL
HHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
ESGMMTLGYLVTVLAMIRVGEKLLQRYGARLPMMAGPVLTAIAIALISCTFLEKALYIGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VFASNVLFGLGLGCYATPSTDTAVANAPENKIGVASGIYKMGSSLGGAMGIAVTASLFAL
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
FLPLGMAHAAQYALWFNAVLCLGAMAVSALLLPRASHS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA