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The map label for this gene is pspA [H]

Identifier: 238894360

GI number: 238894360

Start: 2253443

End: 2254111

Strand: Direct

Name: pspA [H]

Synonym: KP1_2342

Alternate gene names: 238894360

Gene position: 2253443-2254111 (Clockwise)

Preceding gene: 238894358

Following gene: 238894361

Centisome position: 42.93

GC content: 57.7

Gene sequence:

>669_bases
ATGGGTATTTTTTCTCGTTTTGCCGACATCGTGAACGCCAACATCAACTCCCTGCTGGAGAAAGCGGAGGACCCGCAAAA
GCTGGTGCGTCTGATGATCCAGGAGATGGAAGACACGCTGGTTGAGGTGCGTTCGACATCGGCCCGCGCGCTGGCGGAAA
AGAAACAGCTGTCCCGTCGTATTGAGCAGGCGGTCGCCCAGCAGGCGGAATGGCAGGAAAAAGCCGAGCTGGCCCTGCGC
AAAGAGAAAGAGGATCTGGCCCGGGCGGCGCTGATCGAAAAACAAAAGCTGACCGACCTTATCGCTCAGTTGGATCATGA
AGTGCAGCTGGTGGATGAAACCCTGGCGCGGATGAAAAAAGAGATTGGCGAGCTGGAGAACAAACTCAGCGAAACCCGCG
CCCGTCAGCAGGCGCTGGCGCTGCGCCATCAGGCGGCGAGTTCGTCCCGCGATGTGCGTCGCCAGCTGGACAGCGGTAAA
CTGGATGAGGCGATGGCGCGCTTTGAATCCTTTGAGCGTCGCATCGATCAGATGGAAGCGGAAGCGGAGAGCCACCGCTT
TGGCAAGCAGCAGACGCTGGATCAGCAGTTTGCCGAACTGAAAGCGGACGATGAAATCAGCGAGCAGCTGGCGGCGTTGA
AAGCCAAAATGAATCAGTCCAACCAGTAA

Upstream 100 bases:

>100_bases
CCACCCTGTTTAAAATCAATAAGATAAAAAGTTGGCACGCCATTTGTATTAGCTAAGCCACAGGTGAATGCCTGAAACCA
GAACTATGAGGATTGAAATT

Downstream 100 bases:

>100_bases
GTATGATTGCGCGGCGCGGCGTTGCGCCGCGGCTCACCCCCTGTAAGGAGTACACATGAGCATGCTTTTTCTCGCCATTC
CGCTGACGCTGTTTGTGCTG

Product: phage shock protein PspA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MGIFSRFADIVNANINSLLEKAEDPQKLVRLMIQEMEDTLVEVRSTSARALAEKKQLSRRIEQAVAQQAEWQEKAELALR
KEKEDLARAALIEKQKLTDLIAQLDHEVQLVDETLARMKKEIGELENKLSETRARQQALALRHQAASSSRDVRRQLDSGK
LDEAMARFESFERRIDQMEAEAESHRFGKQQTLDQQFAELKADDEISEQLAALKAKMNQSNQ

Sequences:

>Translated_222_residues
MGIFSRFADIVNANINSLLEKAEDPQKLVRLMIQEMEDTLVEVRSTSARALAEKKQLSRRIEQAVAQQAEWQEKAELALR
KEKEDLARAALIEKQKLTDLIAQLDHEVQLVDETLARMKKEIGELENKLSETRARQQALALRHQAASSSRDVRRQLDSGK
LDEAMARFESFERRIDQMEAEAESHRFGKQQTLDQQFAELKADDEISEQLAALKAKMNQSNQ
>Mature_221_residues
GIFSRFADIVNANINSLLEKAEDPQKLVRLMIQEMEDTLVEVRSTSARALAEKKQLSRRIEQAVAQQAEWQEKAELALRK
EKEDLARAALIEKQKLTDLIAQLDHEVQLVDETLARMKKEIGELENKLSETRARQQALALRHQAASSSRDVRRQLDSGKL
DEAMARFESFERRIDQMEAEAESHRFGKQQTLDQQFAELKADDEISEQLAALKAKMNQSNQ

Specific function: The phage shock protein (psp) operon (pspABCDE) may play a significant role in the competition for survival under nutrient- or energy-limited conditions. PspA negatively regulates expression of the pspABCDE promoter and of pspG through negative regulation

COG id: COG1842

COG function: function code KT; Phage shock protein A (IM30), suppresses sigma54-dependent transcription

Gene ontology:

Cell location: Cytoplasm. Cell inner membrane; Peripheral membrane protein; Cytoplasmic side (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PspA/IM30 family [H]

Homologues:

Organism=Escherichia coli, GI2367118, Length=222, Percent_Identity=89.6396396396396, Blast_Score=387, Evalue=1e-109,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014319
- InterPro:   IPR007157 [H]

Pfam domain/function: PF04012 PspA_IM30 [H]

EC number: NA

Molecular weight: Translated: 25444; Mature: 25313

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIFSRFADIVNANINSLLEKAEDPQKLVRLMIQEMEDTLVEVRSTSARALAEKKQLSRR
CCHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEQAVAQQAEWQEKAELALRKEKEDLARAALIEKQKLTDLIAQLDHEVQLVDETLARMKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIGELENKLSETRARQQALALRHQAASSSRDVRRQLDSGKLDEAMARFESFERRIDQMEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
EAESHRFGKQQTLDQQFAELKADDEISEQLAALKAKMNQSNQ
HHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GIFSRFADIVNANINSLLEKAEDPQKLVRLMIQEMEDTLVEVRSTSARALAEKKQLSRR
CHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEQAVAQQAEWQEKAELALRKEKEDLARAALIEKQKLTDLIAQLDHEVQLVDETLARMKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIGELENKLSETRARQQALALRHQAASSSRDVRRQLDSGKLDEAMARFESFERRIDQMEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
EAESHRFGKQQTLDQQFAELKADDEISEQLAALKAKMNQSNQ
HHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]