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The map label for this gene is ansA [H]

Identifier: 238894258

GI number: 238894258

Start: 2145381

End: 2146400

Strand: Reverse

Name: ansA [H]

Synonym: KP1_2229

Alternate gene names: 238894258

Gene position: 2146400-2145381 (Counterclockwise)

Preceding gene: 238894259

Following gene: 238894257

Centisome position: 40.9

GC content: 58.73

Gene sequence:

>1020_bases
ATGCAAAAAAAATCGATTTACGTTGCCTACACCGGTGGGACCATCGGTATGCAGCGCTCTGAACACGGCTATGTACCCGT
TTCCGGCCATCTGCAGCGTCAGCTGGCGCTGATGCCGGAGTTCCATCGTCCGGAGATGCCGGACTTCACGATCCATGAAT
ACCATCCGCTGATGGATTCATCCGATATGACCCCCGAGGACTGGCAGCATATCGCCGACGATATCCGCAGCCATTATGAG
GAGTATGATGGCTTTGTGATCCTCCACGGAACCGACACCATGGCGTTTACCGCCTCGGCGCTGTCGTTCATGCTGGAAAA
CCTTGGTAAGCCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCCGAGCTGCGTTCCGATGGTCAGATTAACCTGCTCA
ACGCCCTGTACGTCGCCGCCAACTATCCGATTAACGAAGTGGCGCTGTTCTTCAACAATCGACTGTTCCGCGGCAACCGC
ACCACCAAAGCCCATGCCGACGGCTTCGACGCTTTCGCCTCGCCAAACCTTGCCCCGCTGCTGGAGGCCGGGATCCATAT
TCGCCGTCTCGGTACTCCGCCGGCGCCGCAGGGTAGCGGAGAGCTTATCGTGCACCCCATCACTCCGCAGCCGATTGGCG
TGGTGACGATTTACCCCGGCATCTCCGCCGACGTGGTGCGTAATTTTCTGCGCCAACCGGTGAAAGCGCTGATCCTCCGC
TCCTATGGCGTCGGCAATGCCCCACAGAATGGCGAATTTATTCAGGTGCTGGCGGAAGCCAGCCAGCGCGGTATTGTGGT
GGTCAATCTGACGCAGTGCATGTCCGGAAAGGTGAACATGGGCGGCTATGCCACCGGCAACGCCCTCGCCCAGGCGGGGG
TCATCAGTGGTTTCGATATGACGGTGGAAGCGACGCTGACCAAGCTACACTATCTGCTTAGCCAGCAGCTGGACGTTGAC
GCCATTCGCGCGGCGATGCAGCAAAATCTGCGTGGCGAACTCACCCCCGACGAGGCTTAA

Upstream 100 bases:

>100_bases
CCCTCTCCTGCCAACACTGCCACAAAGCCCGCGACCGACGCTGTATTTTTTCCTGCGTCTCTTTATACTGCGCCTGTCTA
CGACCAACCTAAGCGATGCT

Downstream 100 bases:

>100_bases
GGAGGACGTATGGCACATCGAGCGCTGTTACTGGTTGATTTGCAGAACGACTTTTGCGCCGGCGGCGCGCTGGCCGTTCC
TGAAGGCGACAGTACCGTCG

Product: cytoplasmic asparaginase I

Products: NA

Alternate protein names: L-asparaginase I; L-ASNase I; L-asparagine amidohydrolase I [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MQKKSIYVAYTGGTIGMQRSEHGYVPVSGHLQRQLALMPEFHRPEMPDFTIHEYHPLMDSSDMTPEDWQHIADDIRSHYE
EYDGFVILHGTDTMAFTASALSFMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVALFFNNRLFRGNR
TTKAHADGFDAFASPNLAPLLEAGIHIRRLGTPPAPQGSGELIVHPITPQPIGVVTIYPGISADVVRNFLRQPVKALILR
SYGVGNAPQNGEFIQVLAEASQRGIVVVNLTQCMSGKVNMGGYATGNALAQAGVISGFDMTVEATLTKLHYLLSQQLDVD
AIRAAMQQNLRGELTPDEA

Sequences:

>Translated_339_residues
MQKKSIYVAYTGGTIGMQRSEHGYVPVSGHLQRQLALMPEFHRPEMPDFTIHEYHPLMDSSDMTPEDWQHIADDIRSHYE
EYDGFVILHGTDTMAFTASALSFMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVALFFNNRLFRGNR
TTKAHADGFDAFASPNLAPLLEAGIHIRRLGTPPAPQGSGELIVHPITPQPIGVVTIYPGISADVVRNFLRQPVKALILR
SYGVGNAPQNGEFIQVLAEASQRGIVVVNLTQCMSGKVNMGGYATGNALAQAGVISGFDMTVEATLTKLHYLLSQQLDVD
AIRAAMQQNLRGELTPDEA
>Mature_339_residues
MQKKSIYVAYTGGTIGMQRSEHGYVPVSGHLQRQLALMPEFHRPEMPDFTIHEYHPLMDSSDMTPEDWQHIADDIRSHYE
EYDGFVILHGTDTMAFTASALSFMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVALFFNNRLFRGNR
TTKAHADGFDAFASPNLAPLLEAGIHIRRLGTPPAPQGSGELIVHPITPQPIGVVTIYPGISADVVRNFLRQPVKALILR
SYGVGNAPQNGEFIQVLAEASQRGIVVVNLTQCMSGKVNMGGYATGNALAQAGVISGFDMTVEATLTKLHYLLSQQLDVD
AIRAAMQQNLRGELTPDEA

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=360, Percent_Identity=47.5, Blast_Score=285, Evalue=6e-77,
Organism=Escherichia coli, GI1788065, Length=338, Percent_Identity=90.8284023668639, Blast_Score=640, Evalue=0.0,
Organism=Escherichia coli, GI1789327, Length=230, Percent_Identity=29.5652173913043, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI193207372, Length=357, Percent_Identity=44.8179271708684, Blast_Score=296, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI17558280, Length=357, Percent_Identity=44.8179271708684, Blast_Score=295, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI17558278, Length=357, Percent_Identity=44.8179271708684, Blast_Score=295, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI193207374, Length=357, Percent_Identity=44.8179271708684, Blast_Score=295, Evalue=2e-80,
Organism=Drosophila melanogaster, GI24639609, Length=371, Percent_Identity=42.8571428571429, Blast_Score=283, Evalue=8e-77,
Organism=Drosophila melanogaster, GI24645530, Length=365, Percent_Identity=42.4657534246575, Blast_Score=276, Evalue=1e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 37053; Mature: 37053

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKKSIYVAYTGGTIGMQRSEHGYVPVSGHLQRQLALMPEFHRPEMPDFTIHEYHPLMDS
CCCCEEEEEEECCEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCC
SDMTPEDWQHIADDIRSHYEEYDGFVILHGTDTMAFTASALSFMLENLGKPVIVTGSQIP
CCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCCC
LAELRSDGQINLLNALYVAANYPINEVALFFNNRLFRGNRTTKAHADGFDAFASPNLAPL
HHHHCCCCCEEEEEEEEEEECCCHHHEEHEECCEEEECCCCCCCCCCCCHHHCCCCHHHH
LEAGIHIRRLGTPPAPQGSGELIVHPITPQPIGVVTIYPGISADVVRNFLRQPVKALILR
HHCCCEEEECCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
SYGVGNAPQNGEFIQVLAEASQRGIVVVNLTQCMSGKVNMGGYATGNALAQAGVISGFDM
HCCCCCCCCCCHHHHHHHHHHCCCEEEEEEHHHHCCCCCCCCEECCHHHHHHCCCCCCCC
TVEATLTKLHYLLSQQLDVDAIRAAMQQNLRGELTPDEA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MQKKSIYVAYTGGTIGMQRSEHGYVPVSGHLQRQLALMPEFHRPEMPDFTIHEYHPLMDS
CCCCEEEEEEECCEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCC
SDMTPEDWQHIADDIRSHYEEYDGFVILHGTDTMAFTASALSFMLENLGKPVIVTGSQIP
CCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCCC
LAELRSDGQINLLNALYVAANYPINEVALFFNNRLFRGNRTTKAHADGFDAFASPNLAPL
HHHHCCCCCEEEEEEEEEEECCCHHHEEHEECCEEEECCCCCCCCCCCCHHHCCCCHHHH
LEAGIHIRRLGTPPAPQGSGELIVHPITPQPIGVVTIYPGISADVVRNFLRQPVKALILR
HHCCCEEEECCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
SYGVGNAPQNGEFIQVLAEASQRGIVVVNLTQCMSGKVNMGGYATGNALAQAGVISGFDM
HCCCCCCCCCCHHHHHHHHHHCCCEEEEEEHHHHCCCCCCCCEECCHHHHHHCCCCCCCC
TVEATLTKLHYLLSQQLDVDAIRAAMQQNLRGELTPDEA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]