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The map label for this gene is sppA [H]

Identifier: 238894259

GI number: 238894259

Start: 2146539

End: 2148392

Strand: Reverse

Name: sppA [H]

Synonym: KP1_2230

Alternate gene names: 238894259

Gene position: 2148392-2146539 (Counterclockwise)

Preceding gene: 238894261

Following gene: 238894258

Centisome position: 40.93

GC content: 60.03

Gene sequence:

>1854_bases
ATGCGAACCCTTTGGCGACTGATTGCCAGCTTTTTTAAATGGACGTGGCGGATACTCAACTTTATTCGCAAACTGGCCCT
CAATGCGATCTTCCTGGTGCTGGTGCTGGTGTGTATCGGCATCTGGAGCCAGTTCAGCAGTACCACCAGCGAACATGCCG
TGCGCGGCGCGCTGTTGCTGGATATTACCGGCGTGGTGGTGGATAAACCATCCGCCAGCAGCAAGCTCGGCGTGATTGGC
CGCCAGCTGTTTGGCGCCAGCTCGGACCGTCTGCAGGAGAATTCCCTGTTCGATATCGTGCAGACCATTCGCCAGGCGAA
GGATGACCGCAATATCACCGGTATTGTGCTGGATTTGAAAAACTTCGTCGGCGGCGACCAGCCATCCATGCAGTACATCG
GCAAAGCGCTGCGCGAATTCCGCGATAGCGGCAAACCGGTGTATGCCGTCGGCAGCAGCTACAGCCAGGGCCAGTACTAC
CTGGCGAGCTTCGCCAACAAAATCTGGCTCTCGCCGCAGGGCGAGGTAGACCTGCACGGCTTCGCTACCAACGGGCTATA
TTACAAATCGCTGCTGGATAAGCTGAAGGTCTCCACCCATGTCTTCCGCGTCGGCACCTATAAATCCGCCGTCGAACCAT
TCATCCGCGATGATATGTCCCCTGCCGCCCGCGAAGCGGATAGCCGCTGGATTGGCGAACTGTGGCAGAACTACCTCAAC
ACCATTGCCGCCAACCGCCAGATCACCGCCCAGCAGCTCTTCCCGGGCGCCCAGGGCATTATCGATGGCCTGCGTAAGGT
GGGCGGCGATACGGCGAAATATGCGCTGGACAATAAGCTGGTGGATGAGCTGGCGACCTCCACGGAAGTGGAAAAGGCGC
TGACCAAACAGTTTGGCTGGAGCAAAGCGGATAACAATTACCGGGCAATCAGCTATTACGACTACAACGTGAAGACGCCG
TCTGATCAGGGTTCCGCCATCGCGGTAATCTTCGCCAACGGCGCCATCATGGACGGTGAAGAGACCCCGGGCAACGTCGG
CGGCGACACCACCGCAGCGCAAATTCGCGATGCGCGCCTCGATCCGAAAATTAAGGCCATCGTTCTGCGCGTAAACAGCC
CGGGCGGCAGCGTAACCGCTTCGGAAATCATTCGTGAAGAGCTGGCGGCAGCCAAAGCCGCCGGTAAACCGGTAGTGGTC
TCGATGGGCGGCATGGCGGCGTCCGGCGGCTACTGGATCTCCACCCCAGCGGATTACATCGTGGCGAACCCAAGCACCCT
CACCGGCTCCATTGGCATCTTTGGGGTGATCAACACCGTGGAAAATACCCTCGGGTCGATTGGCGTCCACACCGACGGCG
TCGCCACGTCGCCGCTGGCGGATGTCTCCAGCACCAAAGCGCTGCCGCCGGAAGTGCAGCAGCTGATGCAGTTGAGCATT
GAGAATGGCTATCAGCGCTTTATCACCCTGGTGGCTAACGCCCGCAAGAGCACGCCGGAGAAAATCGATCAGATTGCCCA
GGGCCACGTCTGGACCGGGGAAGATGCCAAAGCCAACGGCCTGGTCGACAGCCTCGGCGACTTCGACGACGCGGTGGCCA
AAGCCGCCGAGCTGGCGAAGCTGAAAACCTGGCACCTCAACTACTATCAGGAAGAGCCGACCTTCTTCTCGATGGTGCTG
GACAGCCTGACCGGCTCGGTACGCGCCTCGCTGCCGGCGGCCATTCAGGCCTGGCTGCCTGCGCCGGTCGCCGCGGCGGC
CGAGACGGTGAAAGCGGAGAGCGACAAGCTGGCGGCGTTTAACGACCCGCAGAATCGCTATGCCTTCTGCCTGACCTGCG
CTAACATCCGTTAA

Upstream 100 bases:

>100_bases
GTCGGTGATTCCTGCTGACAACCGACGGCTCACTCTTTAGGATAGCCAGACGCGGCGGCCCCTGCGGCCTGCCCTTTTTA
GATAACGTTAGGGAGAAGAC

Downstream 100 bases:

>100_bases
TACGCAGCGGCTGCCCCGTCTCCCGGGGCAGCCTTTTTCCCTCTCCTGCCAACACTGCCACAAAGCCCGCGACCGACGCT
GTATTTTTTCCTGCGTCTCT

Product: protease 4

Products: NA

Alternate protein names: Endopeptidase IV; Protease IV; Signal peptide peptidase [H]

Number of amino acids: Translated: 617; Mature: 617

Protein sequence:

>617_residues
MRTLWRLIASFFKWTWRILNFIRKLALNAIFLVLVLVCIGIWSQFSSTTSEHAVRGALLLDITGVVVDKPSASSKLGVIG
RQLFGASSDRLQENSLFDIVQTIRQAKDDRNITGIVLDLKNFVGGDQPSMQYIGKALREFRDSGKPVYAVGSSYSQGQYY
LASFANKIWLSPQGEVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRDDMSPAAREADSRWIGELWQNYLN
TIAANRQITAQQLFPGAQGIIDGLRKVGGDTAKYALDNKLVDELATSTEVEKALTKQFGWSKADNNYRAISYYDYNVKTP
SDQGSAIAVIFANGAIMDGEETPGNVGGDTTAAQIRDARLDPKIKAIVLRVNSPGGSVTASEIIREELAAAKAAGKPVVV
SMGGMAASGGYWISTPADYIVANPSTLTGSIGIFGVINTVENTLGSIGVHTDGVATSPLADVSSTKALPPEVQQLMQLSI
ENGYQRFITLVANARKSTPEKIDQIAQGHVWTGEDAKANGLVDSLGDFDDAVAKAAELAKLKTWHLNYYQEEPTFFSMVL
DSLTGSVRASLPAAIQAWLPAPVAAAAETVKAESDKLAAFNDPQNRYAFCLTCANIR

Sequences:

>Translated_617_residues
MRTLWRLIASFFKWTWRILNFIRKLALNAIFLVLVLVCIGIWSQFSSTTSEHAVRGALLLDITGVVVDKPSASSKLGVIG
RQLFGASSDRLQENSLFDIVQTIRQAKDDRNITGIVLDLKNFVGGDQPSMQYIGKALREFRDSGKPVYAVGSSYSQGQYY
LASFANKIWLSPQGEVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRDDMSPAAREADSRWIGELWQNYLN
TIAANRQITAQQLFPGAQGIIDGLRKVGGDTAKYALDNKLVDELATSTEVEKALTKQFGWSKADNNYRAISYYDYNVKTP
SDQGSAIAVIFANGAIMDGEETPGNVGGDTTAAQIRDARLDPKIKAIVLRVNSPGGSVTASEIIREELAAAKAAGKPVVV
SMGGMAASGGYWISTPADYIVANPSTLTGSIGIFGVINTVENTLGSIGVHTDGVATSPLADVSSTKALPPEVQQLMQLSI
ENGYQRFITLVANARKSTPEKIDQIAQGHVWTGEDAKANGLVDSLGDFDDAVAKAAELAKLKTWHLNYYQEEPTFFSMVL
DSLTGSVRASLPAAIQAWLPAPVAAAAETVKAESDKLAAFNDPQNRYAFCLTCANIR
>Mature_617_residues
MRTLWRLIASFFKWTWRILNFIRKLALNAIFLVLVLVCIGIWSQFSSTTSEHAVRGALLLDITGVVVDKPSASSKLGVIG
RQLFGASSDRLQENSLFDIVQTIRQAKDDRNITGIVLDLKNFVGGDQPSMQYIGKALREFRDSGKPVYAVGSSYSQGQYY
LASFANKIWLSPQGEVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRDDMSPAAREADSRWIGELWQNYLN
TIAANRQITAQQLFPGAQGIIDGLRKVGGDTAKYALDNKLVDELATSTEVEKALTKQFGWSKADNNYRAISYYDYNVKTP
SDQGSAIAVIFANGAIMDGEETPGNVGGDTTAAQIRDARLDPKIKAIVLRVNSPGGSVTASEIIREELAAAKAAGKPVVV
SMGGMAASGGYWISTPADYIVANPSTLTGSIGIFGVINTVENTLGSIGVHTDGVATSPLADVSSTKALPPEVQQLMQLSI
ENGYQRFITLVANARKSTPEKIDQIAQGHVWTGEDAKANGLVDSLGDFDDAVAKAAELAKLKTWHLNYYQEEPTFFSMVL
DSLTGSVRASLPAAIQAWLPAPVAAAAETVKAESDKLAAFNDPQNRYAFCLTCANIR

Specific function: Digestion of the cleaved signal peptides. This activity is necessary to maintain proper secretion of mature proteins across the membrane [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=618, Percent_Identity=82.6860841423948, Blast_Score=1056, Evalue=0.0,
Organism=Escherichia coli, GI1787527, Length=216, Percent_Identity=27.7777777777778, Blast_Score=76, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004634
- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 66835; Mature: 66835

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTLWRLIASFFKWTWRILNFIRKLALNAIFLVLVLVCIGIWSQFSSTTSEHAVRGALLL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCEEEE
DITGVVVDKPSASSKLGVIGRQLFGASSDRLQENSLFDIVQTIRQAKDDRNITGIVLDLK
EEEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEH
NFVGGDQPSMQYIGKALREFRDSGKPVYAVGSSYSQGQYYLASFANKIWLSPQGEVDLHG
HHCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHEECCCCCCEEEEE
FATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRDDMSPAAREADSRWIGELWQNYLN
EEECCCHHHHHHHHHHHHHHEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
TIAANRQITAQQLFPGAQGIIDGLRKVGGDTAKYALDNKLVDELATSTEVEKALTKQFGW
HHHCCCCEEHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SKADNNYRAISYYDYNVKTPSDQGSAIAVIFANGAIMDGEETPGNVGGDTTAAQIRDARL
CCCCCCEEEEEEEEEEEECCCCCCCEEEEEEECCEEECCCCCCCCCCCCCHHHHHHHCCC
DPKIKAIVLRVNSPGGSVTASEIIREELAAAKAAGKPVVVSMGGMAASGGYWISTPADYI
CCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCEEECCCEEECCCCCEE
VANPSTLTGSIGIFGVINTVENTLGSIGVHTDGVATSPLADVSSTKALPPEVQQLMQLSI
EECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
ENGYQRFITLVANARKSTPEKIDQIAQGHVWTGEDAKANGLVDSLGDFDDAVAKAAELAK
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCEECCCCCCCCCCHHHHCCHHHHHHHHHHHHH
LKTWHLNYYQEEPTFFSMVLDSLTGSVRASLPAAIQAWLPAPVAAAAETVKAESDKLAAF
HHHEECCEECCCCHHHHHHHHHHCCHHHHCCHHHHHHHCCCCHHHHHHHHHCCCCCCEEC
NDPQNRYAFCLTCANIR
CCCCCCEEEEEEECCCC
>Mature Secondary Structure
MRTLWRLIASFFKWTWRILNFIRKLALNAIFLVLVLVCIGIWSQFSSTTSEHAVRGALLL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCEEEE
DITGVVVDKPSASSKLGVIGRQLFGASSDRLQENSLFDIVQTIRQAKDDRNITGIVLDLK
EEEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEH
NFVGGDQPSMQYIGKALREFRDSGKPVYAVGSSYSQGQYYLASFANKIWLSPQGEVDLHG
HHCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHEECCCCCCEEEEE
FATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRDDMSPAAREADSRWIGELWQNYLN
EEECCCHHHHHHHHHHHHHHEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
TIAANRQITAQQLFPGAQGIIDGLRKVGGDTAKYALDNKLVDELATSTEVEKALTKQFGW
HHHCCCCEEHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SKADNNYRAISYYDYNVKTPSDQGSAIAVIFANGAIMDGEETPGNVGGDTTAAQIRDARL
CCCCCCEEEEEEEEEEEECCCCCCCEEEEEEECCEEECCCCCCCCCCCCCHHHHHHHCCC
DPKIKAIVLRVNSPGGSVTASEIIREELAAAKAAGKPVVVSMGGMAASGGYWISTPADYI
CCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCEEECCCEEECCCCCEE
VANPSTLTGSIGIFGVINTVENTLGSIGVHTDGVATSPLADVSSTKALPPEVQQLMQLSI
EECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
ENGYQRFITLVANARKSTPEKIDQIAQGHVWTGEDAKANGLVDSLGDFDDAVAKAAELAK
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCEECCCCCCCCCCHHHHCCHHHHHHHHHHHHH
LKTWHLNYYQEEPTFFSMVLDSLTGSVRASLPAAIQAWLPAPVAAAAETVKAESDKLAAF
HHHEECCEECCCCHHHHHHHHHHCCHHHHCCHHHHHHHCCCCHHHHHHHHHCCCCCCEEC
NDPQNRYAFCLTCANIR
CCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]