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The map label for this gene is ymfC [H]

Identifier: 238894184

GI number: 238894184

Start: 2087358

End: 2088008

Strand: Reverse

Name: ymfC [H]

Synonym: KP1_2145

Alternate gene names: 238894184

Gene position: 2088008-2087358 (Counterclockwise)

Preceding gene: 238894186

Following gene: 238894183

Centisome position: 39.78

GC content: 59.29

Gene sequence:

>651_bases
ATGCGGCAATTGATAAAGCCTGAAAATACCATGCAGAAAACTTCATTTAGAAAACATCGCGTTGAGCGATTCAGCACACA
ACAAGTCACCAGACAACGTAAAGAACGCCAGCCAAAAACTGTGATCTTGTTCAATAAACCCTATGATGTGTTGCCGCAAT
TTACCGATGAAGCCGGGCGCAGCACGCTAAAAGATTTTATTCCGGTGGCGGGCGTCTACGCCGCAGGTCGGCTCGATCGC
GACAGCGAAGGCCTGCTGGTGCTGACCAACGACGGCGCGCTGCAGGCGAAGCTGACCCAGCCGGGTAAGCGCACCGGCAA
GATCTACTATGTTCAGGTGGAAGGCGAACCCACGCCCGAGGCGCTGACCGCCTTGCGCGACGGCGTCACCCTTAACGACG
GCCCCACCCTGCCTGCCGGCGTAGAACTTGTCGAGGAACCCGTCTGGCTGTGGCCGCGCAACCCGCCGATTCGTGAGCGC
AAATCGATTCCCACCCGCTGGTTGAAAATCACGCTCTACGAGGGACGCAACCGCCAGGTGCGCCGGATGACCGCCCACGT
CGGCTTCCCCACCCTGCGGCTGATCCGCTACGCCATGGGCGGCTATACCCTCGACGGACTGGCCAACGGCGACTGGCGCA
AAGCCGAATAA

Upstream 100 bases:

>100_bases
GCGAATCGGTTTGAGCAATTTTTTGTTAATAATTTGTAATGAGCATGTCAATACTACCTGAATGTTTGCGTCCATGAAAG
GCATTCGTAATTAGGCTATA

Downstream 100 bases:

>100_bases
CTGAAAAGGAAAAGCGATGTTTAAACCTCATGTCACCGTTGCCTGCGTGGTTCACGCCCAGGATAAATTTCTCATCGTCG
AAGAAACCATTAACGGCAAA

Product: pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MRQLIKPENTMQKTSFRKHRVERFSTQQVTRQRKERQPKTVILFNKPYDVLPQFTDEAGRSTLKDFIPVAGVYAAGRLDR
DSEGLLVLTNDGALQAKLTQPGKRTGKIYYVQVEGEPTPEALTALRDGVTLNDGPTLPAGVELVEEPVWLWPRNPPIRER
KSIPTRWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGGYTLDGLANGDWRKAE

Sequences:

>Translated_216_residues
MRQLIKPENTMQKTSFRKHRVERFSTQQVTRQRKERQPKTVILFNKPYDVLPQFTDEAGRSTLKDFIPVAGVYAAGRLDR
DSEGLLVLTNDGALQAKLTQPGKRTGKIYYVQVEGEPTPEALTALRDGVTLNDGPTLPAGVELVEEPVWLWPRNPPIRER
KSIPTRWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGGYTLDGLANGDWRKAE
>Mature_216_residues
MRQLIKPENTMQKTSFRKHRVERFSTQQVTRQRKERQPKTVILFNKPYDVLPQFTDEAGRSTLKDFIPVAGVYAAGRLDR
DSEGLLVLTNDGALQAKLTQPGKRTGKIYYVQVEGEPTPEALTALRDGVTLNDGPTLPAGVELVEEPVWLWPRNPPIRER
KSIPTRWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGGYTLDGLANGDWRKAE

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=215, Percent_Identity=83.7209302325581, Blast_Score=373, Evalue=1e-105,
Organism=Escherichia coli, GI1787524, Length=195, Percent_Identity=33.3333333333333, Blast_Score=78, Evalue=4e-16,
Organism=Escherichia coli, GI1790453, Length=181, Percent_Identity=30.3867403314917, Blast_Score=77, Evalue=7e-16,
Organism=Escherichia coli, GI1788510, Length=178, Percent_Identity=33.1460674157303, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 24699; Mature: 24699

Theoretical pI: Translated: 10.64; Mature: 10.64

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQLIKPENTMQKTSFRKHRVERFSTQQVTRQRKERQPKTVILFNKPYDVLPQFTDEAGR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHCCCHHHHHHH
STLKDFIPVAGVYAAGRLDRDSEGLLVLTNDGALQAKLTQPGKRTGKIYYVQVEGEPTPE
HHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEECCCCCCCCEEEEEEECCCCCHH
ALTALRDGVTLNDGPTLPAGVELVEEPVWLWPRNPPIRERKSIPTRWLKITLYEGRNRQV
HHHHHHCCCEECCCCCCCHHHHHHCCCEEECCCCCCCHHHHCCCCEEEEEEEECCCCCHH
RRMTAHVGFPTLRLIRYAMGGYTLDGLANGDWRKAE
HHHHHHCCCHHHHHHHHHHCCEEECCCCCCCCCCCC
>Mature Secondary Structure
MRQLIKPENTMQKTSFRKHRVERFSTQQVTRQRKERQPKTVILFNKPYDVLPQFTDEAGR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHCCCHHHHHHH
STLKDFIPVAGVYAAGRLDRDSEGLLVLTNDGALQAKLTQPGKRTGKIYYVQVEGEPTPE
HHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEECCCCCCCCEEEEEEECCCCCHH
ALTALRDGVTLNDGPTLPAGVELVEEPVWLWPRNPPIRERKSIPTRWLKITLYEGRNRQV
HHHHHHCCCEECCCCCCCHHHHHHCCCEEECCCCCCCHHHHCCCCEEEEEEEECCCCCHH
RRMTAHVGFPTLRLIRYAMGGYTLDGLANGDWRKAE
HHHHHHCCCHHHHHHHHHHCCEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]