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The map label for this gene is purR [H]

Identifier: 238893880

GI number: 238893880

Start: 1752991

End: 1754235

Strand: Direct

Name: purR [H]

Synonym: KP1_1813

Alternate gene names: 238893880

Gene position: 1752991-1754235 (Clockwise)

Preceding gene: 238893877

Following gene: 238893887

Centisome position: 33.4

GC content: 60.88

Gene sequence:

>1245_bases
ATGTTGGACCAATTCAGGCTAGGGCAGAAAAATCGTCTGGCAAAGCGCTATTTATGCAAGTTTTTTCATGAAATGAGCGA
GATCGCATATTTGACAGCGGAATTTGCTTGTAACGTGTGCTTTGGCGATTATGAAAATTCTTTCATTTTTCAATCGGAGC
TGGATCACAAAATGGATAAAAAGCTGCGCGTCGCCGAAATTGCCGCCCGCACCGGGCTATCGCCCAGCACCGTTTCACGC
GTCCTGGCCGGGAAAGCGAATACCAGCGACAAGGCGCGCCGGGCGGTGCTGGACTGCGCCCGCGAGCTGGGTGTCATGCA
GGGGTTGGCCGCCGGGCGTCTGCTGCTCAATCATCTGCTGGTGTTTGCCCCGCAGCGCGCATTCGATGAGCGATCGGATA
TCTTCTACTACCGGGTGATCCAGAGTATCAATAACGCGCTGGCGGCGCATGAAGTGCGCCTGCGCTACTGCGCGCTGGAT
GAGAACGACAGCGACGCCAACCTGTTTCTTGCCCGCATGAATGAACCTGAGACCCAGGCCGCGGTGCTGCTGGGGATCGA
CGATCCGCATATTCATGGCCTGGCGGCGGATCTGGGCAAACCCTGCGTATTGATCAACTGTCGGGATGAAGGGATGCGGC
TGCCGTCCATCGCTCCCGATCATCGGCTGATCGGCCAGTGCGCGGCCCGCTATCTGTTTGATATGGGGCATCGGGCGGTG
ATGAACGTGATGTGCCTGCGCCGCTATACCATGGAGCTGCGGCTGGGGGGGATTCAGGAGGCGTGGCGCGCGCGTAATCT
GCGCTTTACCGCCAGCCGGGATCTTATCAGCGTCGCCAACTTTAGCGCCAGAGAGACGGAAGAGCAGGTGGGCGCCTGGC
TTGACGGCTTACAGGGCAGGCCGTTGCCCACCGCCTTCCTGGTGGGCGGCGATTTTATGGCCGCCGGCACCGTCAGCGCA
TTGCAAAAGCGTGGCCTGCGGGTGCCGCAGGATGTCTCGGTGATGAGCATTGACGGATTTAATCTGGCGGCGATTCAGGA
GGTGCCGCTGACCGCGGTGCATGTGCCGCGGGATGAGCTGGGCAGCGAGGCGGTGCATCTCCTGCAGCAGCGCCTGCTGC
GCCCGGAGGCGCCGCACGGCTCGCTACTGCTGCATGGCACGCTGGTGGTACGCGATTCGGTACGGCGGATCCGGCCGGGC
AAAGGCCATACCGCCGTCGAGCCGCAGGGGCTGTATGACGATTAG

Upstream 100 bases:

>100_bases
GAAAGTGACGGTCTCACTCCCTGAGTGGTCACGTAGATTTATTAACTATTTGAATAGTCTATTTTTTATCTCAGCATTAT
TGGTATAACTAGTATGGGAG

Downstream 100 bases:

>100_bases
ACCGGCATACCCAGCGCCCGTTTGCCGTGGATATTCAGATCCTCCAGGGTGAAGCGCCCCTGCCAGCTGGCTTCATAGTC
CTCGCGCGGGAAATCCCCCG

Product: putative periplasmic binding protein/LacI transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MLDQFRLGQKNRLAKRYLCKFFHEMSEIAYLTAEFACNVCFGDYENSFIFQSELDHKMDKKLRVAEIAARTGLSPSTVSR
VLAGKANTSDKARRAVLDCARELGVMQGLAAGRLLLNHLLVFAPQRAFDERSDIFYYRVIQSINNALAAHEVRLRYCALD
ENDSDANLFLARMNEPETQAAVLLGIDDPHIHGLAADLGKPCVLINCRDEGMRLPSIAPDHRLIGQCAARYLFDMGHRAV
MNVMCLRRYTMELRLGGIQEAWRARNLRFTASRDLISVANFSARETEEQVGAWLDGLQGRPLPTAFLVGGDFMAAGTVSA
LQKRGLRVPQDVSVMSIDGFNLAAIQEVPLTAVHVPRDELGSEAVHLLQQRLLRPEAPHGSLLLHGTLVVRDSVRRIRPG
KGHTAVEPQGLYDD

Sequences:

>Translated_414_residues
MLDQFRLGQKNRLAKRYLCKFFHEMSEIAYLTAEFACNVCFGDYENSFIFQSELDHKMDKKLRVAEIAARTGLSPSTVSR
VLAGKANTSDKARRAVLDCARELGVMQGLAAGRLLLNHLLVFAPQRAFDERSDIFYYRVIQSINNALAAHEVRLRYCALD
ENDSDANLFLARMNEPETQAAVLLGIDDPHIHGLAADLGKPCVLINCRDEGMRLPSIAPDHRLIGQCAARYLFDMGHRAV
MNVMCLRRYTMELRLGGIQEAWRARNLRFTASRDLISVANFSARETEEQVGAWLDGLQGRPLPTAFLVGGDFMAAGTVSA
LQKRGLRVPQDVSVMSIDGFNLAAIQEVPLTAVHVPRDELGSEAVHLLQQRLLRPEAPHGSLLLHGTLVVRDSVRRIRPG
KGHTAVEPQGLYDD
>Mature_414_residues
MLDQFRLGQKNRLAKRYLCKFFHEMSEIAYLTAEFACNVCFGDYENSFIFQSELDHKMDKKLRVAEIAARTGLSPSTVSR
VLAGKANTSDKARRAVLDCARELGVMQGLAAGRLLLNHLLVFAPQRAFDERSDIFYYRVIQSINNALAAHEVRLRYCALD
ENDSDANLFLARMNEPETQAAVLLGIDDPHIHGLAADLGKPCVLINCRDEGMRLPSIAPDHRLIGQCAARYLFDMGHRAV
MNVMCLRRYTMELRLGGIQEAWRARNLRFTASRDLISVANFSARETEEQVGAWLDGLQGRPLPTAFLVGGDFMAAGTVSA
LQKRGLRVPQDVSVMSIDGFNLAAIQEVPLTAVHVPRDELGSEAVHLLQQRLLRPEAPHGSLLLHGTLVVRDSVRRIRPG
KGHTAVEPQGLYDD

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=344, Percent_Identity=25, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1787948, Length=335, Percent_Identity=25.6716417910448, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790194, Length=336, Percent_Identity=25, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 46118; Mature: 46118

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDQFRLGQKNRLAKRYLCKFFHEMSEIAYLTAEFACNVCFGDYENSFIFQSELDHKMDK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCEEEHHHHHHHHHH
KLRVAEIAARTGLSPSTVSRVLAGKANTSDKARRAVLDCARELGVMQGLAAGRLLLNHLL
HHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VFAPQRAFDERSDIFYYRVIQSINNALAAHEVRLRYCALDENDSDANLFLARMNEPETQA
HHCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCEEEEEECCCCCCCE
AVLLGIDDPHIHGLAADLGKPCVLINCRDEGMRLPSIAPDHRLIGQCAARYLFDMGHRAV
EEEEECCCCCHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHH
MNVMCLRRYTMELRLGGIQEAWRARNLRFTASRDLISVANFSARETEEQVGAWLDGLQGR
HHHHHHHHHHHHHHCCCHHHHHHHCCCEEECCCCHHHHHCCCHHHHHHHHHHHHHCCCCC
PLPTAFLVGGDFMAAGTVSALQKRGLRVPQDVSVMSIDGFNLAAIQEVPLTAVHVPRDEL
CCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEEECCCCEEEECCHHHH
GSEAVHLLQQRLLRPEAPHGSLLLHGTLVVRDSVRRIRPGKGHTAVEPQGLYDD
HHHHHHHHHHHHCCCCCCCCCEEEEEEEEEHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLDQFRLGQKNRLAKRYLCKFFHEMSEIAYLTAEFACNVCFGDYENSFIFQSELDHKMDK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCEEEHHHHHHHHHH
KLRVAEIAARTGLSPSTVSRVLAGKANTSDKARRAVLDCARELGVMQGLAAGRLLLNHLL
HHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VFAPQRAFDERSDIFYYRVIQSINNALAAHEVRLRYCALDENDSDANLFLARMNEPETQA
HHCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCEEEEEECCCCCCCE
AVLLGIDDPHIHGLAADLGKPCVLINCRDEGMRLPSIAPDHRLIGQCAARYLFDMGHRAV
EEEEECCCCCHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHH
MNVMCLRRYTMELRLGGIQEAWRARNLRFTASRDLISVANFSARETEEQVGAWLDGLQGR
HHHHHHHHHHHHHHCCCHHHHHHHCCCEEECCCCHHHHHCCCHHHHHHHHHHHHHCCCCC
PLPTAFLVGGDFMAAGTVSALQKRGLRVPQDVSVMSIDGFNLAAIQEVPLTAVHVPRDEL
CCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEEECCCCEEEECCHHHH
GSEAVHLLQQRLLRPEAPHGSLLLHGTLVVRDSVRRIRPGKGHTAVEPQGLYDD
HHHHHHHHHHHHCCCCCCCCCEEEEEEEEEHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA