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The map label for this gene is basR [H]

Identifier: 238893828

GI number: 238893828

Start: 1701455

End: 1702126

Strand: Reverse

Name: basR [H]

Synonym: KP1_1760

Alternate gene names: 238893828

Gene position: 1702126-1701455 (Counterclockwise)

Preceding gene: 238893829

Following gene: 238893827

Centisome position: 32.43

GC content: 56.99

Gene sequence:

>672_bases
ATGAAAATCTTAGTCATTGAAGACGATGCGCTGCTGTTGCAGGGGTTAATTCTGGCGATGCAAAGTGAAGGATACGTGTG
CGACGGCGTCTCCACCGCGCACGAAGCCGCGCTATCGCTGGCCAGCAATCATTACAGCCTGATCGTGCTGGATCTCGGCC
TGCCGGATGAAGACGGGCTGCATTTTCTCTCCCGCATGCGGCGGGAGAAAATGACCCAGCCGGTGCTGATCCTCACCGCC
CGCGATACGCTTGAGGATCGCATTAGCGGTCTCGATACCGGGGCGGATGACTACCTGGTAAAACCCTTTGCCCTGGAGGA
ACTCAATGCGCGCATTCGCGCCCTGCTCCGCCGGCATAACAACCAGGGCGATAATGAAATCAGCGTCGGCAATCTGCGCC
TTAACGTCACTCGCCGCCTGGTGTGGCTGGGTGAAACGGCGCTGGATCTTACGCCGAAAGAGTATGCCCTGCTGTCACGG
CTGATGATGAAAGCCGGTAGCCCGGTGCATCGCGAGATCCTCTACAACGACATTTACAGCTGGGACAACGAGCCGGCGAC
CAATACCCTGGAAGTGCATATTCACAACCTACGCGAAAAGATTGGCAAATCGCGGATCCGCACCGTCCGGGGCTTTGGCT
ATATGCTGGCTAACAACATCGACACGGAATAG

Upstream 100 bases:

>100_bases
AGGATAATCTGTTCTCCACCCTGCTCGGCCTGCTCGGCGTCAGCACCCGCGAGTACCAGGCGGCAGATGATATCTTAACG
CCATGTAGAGAGGCTGGTTG

Downstream 100 bases:

>100_bases
ACCATGGCTCTGTTTGCAACTGAAACCTGGACCATGCGCCACCGGCTGCTGCTGACCATCGGCGCCATCCTGGTGGTCTG
CCAGCTGATAAGCGTCTTCT

Product: DNA-binding transcriptional regulator BasR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MKILVIEDDALLLQGLILAMQSEGYVCDGVSTAHEAALSLASNHYSLIVLDLGLPDEDGLHFLSRMRREKMTQPVLILTA
RDTLEDRISGLDTGADDYLVKPFALEELNARIRALLRRHNNQGDNEISVGNLRLNVTRRLVWLGETALDLTPKEYALLSR
LMMKAGSPVHREILYNDIYSWDNEPATNTLEVHIHNLREKIGKSRIRTVRGFGYMLANNIDTE

Sequences:

>Translated_223_residues
MKILVIEDDALLLQGLILAMQSEGYVCDGVSTAHEAALSLASNHYSLIVLDLGLPDEDGLHFLSRMRREKMTQPVLILTA
RDTLEDRISGLDTGADDYLVKPFALEELNARIRALLRRHNNQGDNEISVGNLRLNVTRRLVWLGETALDLTPKEYALLSR
LMMKAGSPVHREILYNDIYSWDNEPATNTLEVHIHNLREKIGKSRIRTVRGFGYMLANNIDTE
>Mature_223_residues
MKILVIEDDALLLQGLILAMQSEGYVCDGVSTAHEAALSLASNHYSLIVLDLGLPDEDGLHFLSRMRREKMTQPVLILTA
RDTLEDRISGLDTGADDYLVKPFALEELNARIRALLRRHNNQGDNEISVGNLRLNVTRRLVWLGETALDLTPKEYALLSR
LMMKAGSPVHREILYNDIYSWDNEPATNTLEVHIHNLREKIGKSRIRTVRGFGYMLANNIDTE

Specific function: Member of the two-component regulatory system BasS/BasR. BasR induces the transcription of the ugd, ais, arnBCADTEF and eptA-basRS loci, all involved in resistance to polymyxin [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790552, Length=219, Percent_Identity=78.0821917808219, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI1789402, Length=219, Percent_Identity=44.2922374429224, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI87082012, Length=218, Percent_Identity=38.5321100917431, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1787375, Length=220, Percent_Identity=35.4545454545455, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1786784, Length=225, Percent_Identity=36, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI1786599, Length=226, Percent_Identity=35.8407079646018, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI1786911, Length=219, Percent_Identity=33.3333333333333, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1789809, Length=230, Percent_Identity=33.9130434782609, Blast_Score=120, Evalue=6e-29,
Organism=Escherichia coli, GI1788394, Length=225, Percent_Identity=33.3333333333333, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI2367329, Length=232, Percent_Identity=36.6379310344828, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1790860, Length=221, Percent_Identity=31.2217194570136, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787229, Length=226, Percent_Identity=31.858407079646, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI1790863, Length=233, Percent_Identity=27.8969957081545, Blast_Score=81, Evalue=5e-17,
Organism=Escherichia coli, GI1788550, Length=108, Percent_Identity=30.5555555555556, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI145693140, Length=188, Percent_Identity=27.6595744680851, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1790299, Length=120, Percent_Identity=29.1666666666667, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25192; Mature: 25192

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVIEDDALLLQGLILAMQSEGYVCDGVSTAHEAALSLASNHYSLIVLDLGLPDEDGL
CEEEEECCCHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHCCCEEEEEEECCCCCCCHH
HFLSRMRREKMTQPVLILTARDTLEDRISGLDTGADDYLVKPFALEELNARIRALLRRHN
HHHHHHHHHHCCCCEEEEEECHHHHHHHHCCCCCCCCCEECCHHHHHHHHHHHHHHHHHC
NQGDNEISVGNLRLNVTRRLVWLGETALDLTPKEYALLSRLMMKAGSPVHREILYNDIYS
CCCCCEEEEEEEEEEEEEEEEEECCHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
WDNEPATNTLEVHIHNLREKIGKSRIRTVRGFGYMLANNIDTE
CCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKILVIEDDALLLQGLILAMQSEGYVCDGVSTAHEAALSLASNHYSLIVLDLGLPDEDGL
CEEEEECCCHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHCCCEEEEEEECCCCCCCHH
HFLSRMRREKMTQPVLILTARDTLEDRISGLDTGADDYLVKPFALEELNARIRALLRRHN
HHHHHHHHHHCCCCEEEEEECHHHHHHHHCCCCCCCCCEECCHHHHHHHHHHHHHHHHHC
NQGDNEISVGNLRLNVTRRLVWLGETALDLTPKEYALLSRLMMKAGSPVHREILYNDIYS
CCCCCEEEEEEEEEEEEEEEEEECCHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
WDNEPATNTLEVHIHNLREKIGKSRIRTVRGFGYMLANNIDTE
CCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8282725; 7610040; 9278503 [H]