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The map label for this gene is eptA [H]

Identifier: 238893829

GI number: 238893829

Start: 1702126

End: 1703775

Strand: Reverse

Name: eptA [H]

Synonym: KP1_1761

Alternate gene names: 238893829

Gene position: 1703775-1702126 (Counterclockwise)

Preceding gene: 238893830

Following gene: 238893828

Centisome position: 32.46

GC content: 55.39

Gene sequence:

>1650_bases
GTGTGCATTATGTCGTTATTGCCTCTGCGTCGGCCGGTGGTCAGCCGCACGACTTATCTGATTCTCTTTGCTTGCTATAT
CGGTATCTGTCTCAACCTGGCTTTTTATCGCCAGGTTTTCCCGGTACTGCCGGTAAATAGCCTGCATAACTGGCTGGTAT
TCCTCAGTATGCCGATCGTGGCCATTAGCGTGATGAATATCCTCACCACTCTCGCCTCGTTCCTGAAGCTGGACCGGCTG
GTGATTAGCCTGTTCATTCTGCTCAGCGCCTCGGCGCAATACTTCATCTGGAACTTTGGCGTGGTGATCGACCGCAGCAT
GATCACCAATATCCTCGACACCACTCCGGCGGAAAGCTTTGCTCTACTCTCCGGCGAAATGATTGCCGTCCTCGGCCTTT
CCGGGGTTTTGGCGGTATTCGTCGCCTGGTGGGTGAAAATCCGTAAGCCCGCCACGCGGTGGCGCGGCGCGGCGATGCGC
CTGCTCAATATCGCCGTTTCGGCGCTGCTGATTATTCTGGTGGCCGCGCTGTTCTACAAAGATTACGCCTCGGTGTTCCG
TAACAACAAAGAGCTGGTGAAATCCCTGAGCCCTTCCAACAGCATTGTGGCCGTGAACTCCTGGTATGCTCATCATCGGA
TGGACAACCTGCCGCTGGTGAAGATTGGCGAAGATGCCACGCAGAAAGCGGTGATGCATAACGCTCCCCGTAAGAACCTG
ACGATCGTCGTGCTCGGCGAGACTTCGCGAGCGGATAACTTCTCACTGGGCGGCTATTCGCGCGATACCAACCCGCTGAT
GCGGCAGGACGGCGTGATCTATTTTCCGCATACCACTTCCTGCGGCACGGCAACCGCAGTTTCCGTGCCGTGCATGTTCT
CCAATATGCCGCGCGCGCATTATGACGAAGAGCTGGCGCACCATCAGGAAGGCGTCCTGGATATTCTACAACGCGCAGGG
ATCCAGGTACTGTGGAACGATAACGACGGCGGCTGCAAAGGCGCCTGCGACCGGGTACCACACCAGAACGTCACCGACCT
GAAGCTCACCGGGCAGTGCATCGATGGCGAGTGCTACGATGACGTCCTGTTCCATAATCTCGACAGCTATATCGATAACC
TGCAACAGGATGGCATTATTGTGCTGCATACCATCGGCAGCCACGGTCCGACGTACTACAATCGCTATCCAGCGGCGTTT
CGCAAATTCACGCCGACCTGCGACACCAATGAGATCCAGGGCTGTACCCGGGAGCAGTTGACCAATACCTATGACAACAC
CATCCTTTATGTCGACTATGTCGTTGATAAAGCGATAAAACTACTGCAATCTAAACAGGATAAATTTACCACTAGCCTGG
TTTATTTGTCCGACCACGGCGAATCGCTGGGAGAAGATGGCGTCTATTTGCATGGTCTGCCGTATTCCATCGCGCCGGAT
ACGCAGAAACATGTGCCGATGGCGCTGTGGCTCTCCGCCGACTACCAGCAGCGCTACGGTATTTCCGCGCACTGTCTGCA
GCAGCGGGCGCAAAAAGAGAATTACTCGCAGGATAATCTGTTCTCCACCCTGCTCGGCCTGCTCGGCGTCAGCACCCGCG
AGTACCAGGCGGCAGATGATATCTTAACGCCATGTAGAGAGGCTGGTTGA

Upstream 100 bases:

>100_bases
GCTGATCCTGCGCGCCGGAGGCGGCCCTCTTCGGCGCACCTTAAAATCTTAAGGTTGTCTTAAAATAGGGCTGGCACTAT
ACCCTCATCTTTTTCTGTGG

Downstream 100 bases:

>100_bases
TGAAAATCTTAGTCATTGAAGACGATGCGCTGCTGTTGCAGGGGTTAATTCTGGCGATGCAAAGTGAAGGATACGTGTGC
GACGGCGTCTCCACCGCGCA

Product: putative cell division protein

Products: NA

Alternate protein names: Polymyxin resistance protein pmrC [H]

Number of amino acids: Translated: 549; Mature: 549

Protein sequence:

>549_residues
MCIMSLLPLRRPVVSRTTYLILFACYIGICLNLAFYRQVFPVLPVNSLHNWLVFLSMPIVAISVMNILTTLASFLKLDRL
VISLFILLSASAQYFIWNFGVVIDRSMITNILDTTPAESFALLSGEMIAVLGLSGVLAVFVAWWVKIRKPATRWRGAAMR
LLNIAVSALLIILVAALFYKDYASVFRNNKELVKSLSPSNSIVAVNSWYAHHRMDNLPLVKIGEDATQKAVMHNAPRKNL
TIVVLGETSRADNFSLGGYSRDTNPLMRQDGVIYFPHTTSCGTATAVSVPCMFSNMPRAHYDEELAHHQEGVLDILQRAG
IQVLWNDNDGGCKGACDRVPHQNVTDLKLTGQCIDGECYDDVLFHNLDSYIDNLQQDGIIVLHTIGSHGPTYYNRYPAAF
RKFTPTCDTNEIQGCTREQLTNTYDNTILYVDYVVDKAIKLLQSKQDKFTTSLVYLSDHGESLGEDGVYLHGLPYSIAPD
TQKHVPMALWLSADYQQRYGISAHCLQQRAQKENYSQDNLFSTLLGLLGVSTREYQAADDILTPCREAG

Sequences:

>Translated_549_residues
MCIMSLLPLRRPVVSRTTYLILFACYIGICLNLAFYRQVFPVLPVNSLHNWLVFLSMPIVAISVMNILTTLASFLKLDRL
VISLFILLSASAQYFIWNFGVVIDRSMITNILDTTPAESFALLSGEMIAVLGLSGVLAVFVAWWVKIRKPATRWRGAAMR
LLNIAVSALLIILVAALFYKDYASVFRNNKELVKSLSPSNSIVAVNSWYAHHRMDNLPLVKIGEDATQKAVMHNAPRKNL
TIVVLGETSRADNFSLGGYSRDTNPLMRQDGVIYFPHTTSCGTATAVSVPCMFSNMPRAHYDEELAHHQEGVLDILQRAG
IQVLWNDNDGGCKGACDRVPHQNVTDLKLTGQCIDGECYDDVLFHNLDSYIDNLQQDGIIVLHTIGSHGPTYYNRYPAAF
RKFTPTCDTNEIQGCTREQLTNTYDNTILYVDYVVDKAIKLLQSKQDKFTTSLVYLSDHGESLGEDGVYLHGLPYSIAPD
TQKHVPMALWLSADYQQRYGISAHCLQQRAQKENYSQDNLFSTLLGLLGVSTREYQAADDILTPCREAG
>Mature_549_residues
MCIMSLLPLRRPVVSRTTYLILFACYIGICLNLAFYRQVFPVLPVNSLHNWLVFLSMPIVAISVMNILTTLASFLKLDRL
VISLFILLSASAQYFIWNFGVVIDRSMITNILDTTPAESFALLSGEMIAVLGLSGVLAVFVAWWVKIRKPATRWRGAAMR
LLNIAVSALLIILVAALFYKDYASVFRNNKELVKSLSPSNSIVAVNSWYAHHRMDNLPLVKIGEDATQKAVMHNAPRKNL
TIVVLGETSRADNFSLGGYSRDTNPLMRQDGVIYFPHTTSCGTATAVSVPCMFSNMPRAHYDEELAHHQEGVLDILQRAG
IQVLWNDNDGGCKGACDRVPHQNVTDLKLTGQCIDGECYDDVLFHNLDSYIDNLQQDGIIVLHTIGSHGPTYYNRYPAAF
RKFTPTCDTNEIQGCTREQLTNTYDNTILYVDYVVDKAIKLLQSKQDKFTTSLVYLSDHGESLGEDGVYLHGLPYSIAPD
TQKHVPMALWLSADYQQRYGISAHCLQQRAQKENYSQDNLFSTLLGLLGVSTREYQAADDILTPCREAG

Specific function: Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin [H]

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082372, Length=539, Percent_Identity=70.8719851576994, Blast_Score=788, Evalue=0.0,
Organism=Escherichia coli, GI87082286, Length=552, Percent_Identity=25.9057971014493, Blast_Score=149, Evalue=6e-37,
Organism=Escherichia coli, GI87082223, Length=452, Percent_Identity=24.7787610619469, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 61387; Mature: 61387

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCIMSLLPLRRPVVSRTTYLILFACYIGICLNLAFYRQVFPVLPVNSLHNWLVFLSMPIV
CCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
AISVMNILTTLASFLKLDRLVISLFILLSASAQYFIWNFGVVIDRSMITNILDTTPAESF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHCCCCCHH
ALLSGEMIAVLGLSGVLAVFVAWWVKIRKPATRWRGAAMRLLNIAVSALLIILVAALFYK
HHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DYASVFRNNKELVKSLSPSNSIVAVNSWYAHHRMDNLPLVKIGEDATQKAVMHNAPRKNL
HHHHHHHCCHHHHHHCCCCCCEEEEECHHHHHCCCCCCEEEECCHHHHHHHHHCCCCCCE
TIVVLGETSRADNFSLGGYSRDTNPLMRQDGVIYFPHTTSCGTATAVSVPCMFSNMPRAH
EEEEEECCCCCCCCCCCCCCCCCCCHHHCCCEEEECCCCCCCCCHHEECCHHHCCCCCCH
YDEELAHHQEGVLDILQRAGIQVLWNDNDGGCKGACDRVPHQNVTDLKLTGQCIDGECYD
HHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHCCCCCCCCCEEEECEEECCCHHH
DVLFHNLDSYIDNLQQDGIIVLHTIGSHGPTYYNRYPAAFRKFTPTCDTNEIQGCTREQL
HHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHCCCHHHHHCCCCCCCHHHCCCCHHHH
TNTYDNTILYVDYVVDKAIKLLQSKQDKFTTSLVYLSDHGESLGEDGVYLHGLPYSIAPD
HHHHCCEEEEHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCEEEECCCCCCCCC
TQKHVPMALWLSADYQQRYGISAHCLQQRAQKENYSQDNLFSTLLGLLGVSTREYQAADD
CCCCCCEEEEEECCHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHH
ILTPCREAG
HHHHHHCCC
>Mature Secondary Structure
MCIMSLLPLRRPVVSRTTYLILFACYIGICLNLAFYRQVFPVLPVNSLHNWLVFLSMPIV
CCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
AISVMNILTTLASFLKLDRLVISLFILLSASAQYFIWNFGVVIDRSMITNILDTTPAESF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHCCCCCHH
ALLSGEMIAVLGLSGVLAVFVAWWVKIRKPATRWRGAAMRLLNIAVSALLIILVAALFYK
HHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DYASVFRNNKELVKSLSPSNSIVAVNSWYAHHRMDNLPLVKIGEDATQKAVMHNAPRKNL
HHHHHHHCCHHHHHHCCCCCCEEEEECHHHHHCCCCCCEEEECCHHHHHHHHHCCCCCCE
TIVVLGETSRADNFSLGGYSRDTNPLMRQDGVIYFPHTTSCGTATAVSVPCMFSNMPRAH
EEEEEECCCCCCCCCCCCCCCCCCCHHHCCCEEEECCCCCCCCCHHEECCHHHCCCCCCH
YDEELAHHQEGVLDILQRAGIQVLWNDNDGGCKGACDRVPHQNVTDLKLTGQCIDGECYD
HHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHCCCCCCCCCEEEECEEECCCHHH
DVLFHNLDSYIDNLQQDGIIVLHTIGSHGPTYYNRYPAAFRKFTPTCDTNEIQGCTREQL
HHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHCCCHHHHHCCCCCCCHHHCCCCHHHH
TNTYDNTILYVDYVVDKAIKLLQSKQDKFTTSLVYLSDHGESLGEDGVYLHGLPYSIAPD
HHHHCCEEEEHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCEEEECCCCCCCCC
TQKHVPMALWLSADYQQRYGISAHCLQQRAQKENYSQDNLFSTLLGLLGVSTREYQAADD
CCCCCCEEEEEECCHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHH
ILTPCREAG
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8391535; 11677609 [H]