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The map label for this gene is leuS [H]

Identifier: 238893709

GI number: 238893709

Start: 1569531

End: 1572137

Strand: Reverse

Name: leuS [H]

Synonym: KP1_1630

Alternate gene names: 238893709

Gene position: 1572137-1569531 (Counterclockwise)

Preceding gene: 238893711

Following gene: 238893708

Centisome position: 29.95

GC content: 57.92

Gene sequence:

>2607_bases
TTGAAAACAGGACCACTGGCTGCCATGCAAGAGCAATACCGCCCGGAAGAGATAGAATCGAAAGTCCAGCTTCACTGGGA
CGAAAATCGTACATTTGAAGTAACTGAAGACGAGAGCAAAGAGAAGTATTACTGCCTGTCGATGCTTCCTTATCCTTCTG
GTCGACTACACATGGGTCACGTGCGTAACTACACCATCGGCGACGTGATTGCCCGCTACCAGCGCATGCTGGGCAAAAAC
GTCCTGCAGCCGATCGGCTGGGATGCGTTCGGTCTGCCGGCAGAAGGGGCGGCGGTGAAAAACAACACCGCGCCAGCGCC
GTGGACCTACGACAACATCGCCTACATGAAAAACCAGCTCAAAATGCTGGGCTTCGGCTATGACTGGAGCCGCGAGCTGG
CGACCTGCACCCCGGAATACTACCGCTGGGAGCAGAAGTTCTTCACCGAGCTGTACAAAAAAGGCCTGGTGTACAAAAAA
ACCTCCGCAGTGAACTGGTGCCCGAATGACCAGACCGTGCTGGCCAACGAACAGGTTATCGACGGCTGCTGCTGGCGCTG
CGATACCAAGGTCGAGCGTAAAGAGATCCCGCAGTGGTTTATTAAAATCACCGCCTACGCCGATGAACTGCTGAACGATC
TGGATAAGCTGGACCACTGGCCGGACACCGTGAAGACCATGCAGCGCAACTGGATCGGCCGTTCGGAAGGGGTAGAAATC
TCCTTTGACGTCAACGACTACGCTGACAAACTGACTGTCTACACCACCCGTCCGGACACCTTTATGGGTTGTACCTACCT
GGCCGTCGCGGCGGGTCACCCGCTGGCGCAGCAGGCTGCGGCGAACAATCCGGCGCTGGCCGCCTTTATCGACGAGTGCC
GCAATACCAAAGTGGCCGAAGCCGACATGGCGACGATGGAGAAAAAAGGCGTCGATACCGGCTTTAAAGCCATTCATCCG
CTGACCGGTGAACAAATTCCGGTCTGGGCGGCAAACTTTGTGCTGATGGAATACGGCACCGGCGCGGTAATGGCCGTTCC
GGGTCACGACCAGCGCGACTACGAGTTCGCCAGCAAGTACGGTCTGAACATTAAGCCGGTTATCCTCGCTGCCGACGGCT
CTGAGCCGGATCTCTCCGAGCAGGCGCTGACCGAGAAAGGCGTCCTGTTTAACTCCGGCGAGTTCAGCGGTCTTGATTAT
GAAGCGGGCTTCAACGCCATTGCCGACAAACTGACCGCCATGGGCGTGGGCGAGCGTAAAGTTAACTACCGTCTGCGCGA
CTGGGGCGTATCCCGTCAGCGCTACTGGGGCGCGCCGATCCCGATGGTGACCCTGGAAGATGGCACCGTGCTGCCGACCC
CGGAAGATCAGCTGCCGGTGATCCTGCCGGAAGACGTGGTCATGGACGGCATCACCAGCCCGATCAAAGCCGATCCTGAA
TGGGCCAAGACCACCGTCAACGGCCAGCCGGCGCTGCGCGAAACCGACACCTTTGACACCTTTATGGAATCCTCCTGGTA
CTATGCGCGCTACACCTGCCCGCAGTATCAGGAAGGAATGCTGGATTCGAAAGCGGCCAACTACTGGCTGCCGGTAGATA
TCTACATCGGCGGTATCGAACACGCCATCATGCACCTGCTCTACTTCCGCTTCTTCCATAAGCTGATGCGCGACGCGGGC
ATGGTGAACTCCGATGAGCCGGCGAAACAGCTGCTGTGCCAGGGCATGGTGCTGGCTGACGCGTTCTACTATGTGGGCGA
AAACGGCGAACGTAACTGGGTTTCCCCGGTTGACGCCATCGTTGAGCGCGACGAAAAAGGCCGCATCGTGAAGGCCAAAG
ACGCGGCGGGCCACGAGCTGGTGTACACCGGCATGAGCAAGATGTCCAAGTCGAAAAACAACGGCATCGACCCGCAGGTG
ATGGTTGAGCGCTACGGCGCGGATACCGTGCGTCTGTTCATGATGTTTGCGTCCCCGGCGGATATGACCCTCGAGTGGCA
GGAATCTGGCGTTGAAGGGGCGAACCGCTTCCTGAAACGCGTCTGGAAACTGGTCTACGAGCACACCACTAAAGGAGAGG
TTGCGGCACTGAACGTGGCGGCTCTGAGCGAAGATCAGAAAGCGCTGCGTCGCGATATCCACAAAACCATCGCCAAAGTG
ACCGATGACATCGGCCGTCGTCAGACCTTCAACACCGCCATCGCGGCAATCATGGAGCTGATGAACAAGCTGGCGAAAGC
GCCGCAGGAAGATGAGCAGGATCGTGCGCTGATGCAGGAAGCTCTGCTGGCGGTGGTGCGTATGCTCAACCCGTTCACTC
CGCACGCCAGCTTCACCCTGTGGCGTGAGCTGAACGGCGAAGGCGATATCGACAACGCGCCGTGGCCGGTAGCGGATGAG
AGCGCGATGGTGGAAGACAGCACCCTCGTCGTCGTCCAGGTCAATGGCAAAGTTCGTGGTAAAATTACCGTTGCGGTAGA
TGCAACTGAAGAGCAGGTTCGCGAGCGCGCCGGCCAGGAACATCTGGTGGCGAAATATCTCGACGGTAAAACCGTGCGTA
AAGTGATTTACGTTCCGGGTAAACTGCTCAATCTGGTCGTTGGCTAA

Upstream 100 bases:

>100_bases
TGCCGATGTTGATGTGCGAAAAGCTGTTTTGACGTGGGCGTTTGGGCTATGCTATGCCGATCTGAAATAACACATCCATT
GGCTACACTTTGTAGCTGTA

Downstream 100 bases:

>100_bases
GCGCGGGAGGAAGCGTGCGATATCTGGTAACAATGTTGTTATCTCTGGCGGTGCTCGTCACCGCCGGCTGTGGCTGGCAT
TTGCGTAGCACTACCAAGGT

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS [H]

Number of amino acids: Translated: 868; Mature: 868

Protein sequence:

>868_residues
MKTGPLAAMQEQYRPEEIESKVQLHWDENRTFEVTEDESKEKYYCLSMLPYPSGRLHMGHVRNYTIGDVIARYQRMLGKN
VLQPIGWDAFGLPAEGAAVKNNTAPAPWTYDNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKK
TSAVNWCPNDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKTMQRNWIGRSEGVEI
SFDVNDYADKLTVYTTRPDTFMGCTYLAVAAGHPLAQQAAANNPALAAFIDECRNTKVAEADMATMEKKGVDTGFKAIHP
LTGEQIPVWAANFVLMEYGTGAVMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSEQALTEKGVLFNSGEFSGLDY
EAGFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVLPTPEDQLPVILPEDVVMDGITSPIKADPE
WAKTTVNGQPALRETDTFDTFMESSWYYARYTCPQYQEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAG
MVNSDEPAKQLLCQGMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSKNNGIDPQV
MVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTTKGEVAALNVAALSEDQKALRRDIHKTIAKV
TDDIGRRQTFNTAIAAIMELMNKLAKAPQEDEQDRALMQEALLAVVRMLNPFTPHASFTLWRELNGEGDIDNAPWPVADE
SAMVEDSTLVVVQVNGKVRGKITVAVDATEEQVRERAGQEHLVAKYLDGKTVRKVIYVPGKLLNLVVG

Sequences:

>Translated_868_residues
MKTGPLAAMQEQYRPEEIESKVQLHWDENRTFEVTEDESKEKYYCLSMLPYPSGRLHMGHVRNYTIGDVIARYQRMLGKN
VLQPIGWDAFGLPAEGAAVKNNTAPAPWTYDNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKK
TSAVNWCPNDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKTMQRNWIGRSEGVEI
SFDVNDYADKLTVYTTRPDTFMGCTYLAVAAGHPLAQQAAANNPALAAFIDECRNTKVAEADMATMEKKGVDTGFKAIHP
LTGEQIPVWAANFVLMEYGTGAVMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSEQALTEKGVLFNSGEFSGLDY
EAGFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVLPTPEDQLPVILPEDVVMDGITSPIKADPE
WAKTTVNGQPALRETDTFDTFMESSWYYARYTCPQYQEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAG
MVNSDEPAKQLLCQGMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSKNNGIDPQV
MVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTTKGEVAALNVAALSEDQKALRRDIHKTIAKV
TDDIGRRQTFNTAIAAIMELMNKLAKAPQEDEQDRALMQEALLAVVRMLNPFTPHASFTLWRELNGEGDIDNAPWPVADE
SAMVEDSTLVVVQVNGKVRGKITVAVDATEEQVRERAGQEHLVAKYLDGKTVRKVIYVPGKLLNLVVG
>Mature_868_residues
MKTGPLAAMQEQYRPEEIESKVQLHWDENRTFEVTEDESKEKYYCLSMLPYPSGRLHMGHVRNYTIGDVIARYQRMLGKN
VLQPIGWDAFGLPAEGAAVKNNTAPAPWTYDNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKK
TSAVNWCPNDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKTMQRNWIGRSEGVEI
SFDVNDYADKLTVYTTRPDTFMGCTYLAVAAGHPLAQQAAANNPALAAFIDECRNTKVAEADMATMEKKGVDTGFKAIHP
LTGEQIPVWAANFVLMEYGTGAVMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSEQALTEKGVLFNSGEFSGLDY
EAGFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVLPTPEDQLPVILPEDVVMDGITSPIKADPE
WAKTTVNGQPALRETDTFDTFMESSWYYARYTCPQYQEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAG
MVNSDEPAKQLLCQGMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSKNNGIDPQV
MVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTTKGEVAALNVAALSEDQKALRRDIHKTIAKV
TDDIGRRQTFNTAIAAIMELMNKLAKAPQEDEQDRALMQEALLAVVRMLNPFTPHASFTLWRELNGEGDIDNAPWPVADE
SAMVEDSTLVVVQVNGKVRGKITVAVDATEEQVRERAGQEHLVAKYLDGKTVRKVIYVPGKLLNLVVG

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7661872, Length=863, Percent_Identity=36.5005793742758, Blast_Score=515, Evalue=1e-146,
Organism=Escherichia coli, GI1786861, Length=860, Percent_Identity=95.5813953488372, Blast_Score=1725, Evalue=0.0,
Organism=Escherichia coli, GI1790708, Length=393, Percent_Identity=26.972010178117, Blast_Score=117, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI71997517, Length=834, Percent_Identity=30.2158273381295, Blast_Score=352, Evalue=7e-97,
Organism=Caenorhabditis elegans, GI71997510, Length=842, Percent_Identity=30.166270783848, Blast_Score=352, Evalue=7e-97,
Organism=Caenorhabditis elegans, GI212645227, Length=369, Percent_Identity=27.3712737127371, Blast_Score=131, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71980946, Length=151, Percent_Identity=28.476821192053, Blast_Score=67, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6323414, Length=813, Percent_Identity=37.2693726937269, Blast_Score=506, Evalue=1e-144,
Organism=Saccharomyces cerevisiae, GI6321531, Length=387, Percent_Identity=26.8733850129199, Blast_Score=101, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6325217, Length=357, Percent_Identity=22.96918767507, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21355409, Length=881, Percent_Identity=33.3711691259932, Blast_Score=453, Evalue=1e-127,
Organism=Drosophila melanogaster, GI281366294, Length=471, Percent_Identity=22.9299363057325, Blast_Score=78, Evalue=3e-14,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008 [H]

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1 [H]

EC number: =6.1.1.4 [H]

Molecular weight: Translated: 97826; Mature: 97826

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTGPLAAMQEQYRPEEIESKVQLHWDENRTFEVTEDESKEKYYCLSMLPYPSGRLHMGH
CCCCCCHHHHHHCCCHHHCCCEEEEECCCCEEEECCCCCCCCEEEEEECCCCCCCEEECC
VRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTYDNIAYMKNQL
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHH
KMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCPNDQTVLANEQVI
HHEECCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCEEEECCCCEECCCCCEEEECHHHH
DGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKTMQRNWIGRSEGVEI
HHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEE
SFDVNDYADKLTVYTTRPDTFMGCTYLAVAAGHPLAQQAAANNPALAAFIDECRNTKVAE
EEECCCCCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHCCCEECH
ADMATMEKKGVDTGFKAIHPLTGEQIPVWAANFVLMEYGTGAVMAVPGHDQRDYEFASKY
HHHHHHHHCCCCCCHHHHCCCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCCHHHHHHC
GLNIKPVILAADGSEPDLSEQALTEKGVLFNSGEFSGLDYEAGFNAIADKLTAMGVGERK
CCCCEEEEEECCCCCCCCHHHHHHHCCCEEECCCCCCCCHHCCHHHHHHHHHHCCCCCHH
VNYRLRDWGVSRQRYWGAPIPMVTLEDGTVLPTPEDQLPVILPEDVVMDGITSPIKADPE
HCEEEHHCCCCCHHCCCCCCCEEEECCCEECCCCCCCCCEECCHHHHHHCCCCCCCCCCC
WAKTTVNGQPALRETDTFDTFMESSWYYARYTCPQYQEGMLDSKAANYWLPVDIYIGGIE
HHHEECCCCCCCCCCCHHHHHHHCCCEEEEECCCHHHHCCCCCCCCCEEEEEEEEECCHH
HAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQGMVLADAFYYVGENGERNWVSPVDAI
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHEECCCCCCCCCCHHHHH
VERDEKGRIVKAKDAAGHELVYTGMSKMSKSKNNGIDPQVMVERYGADTVRLFMMFASPA
HCCCCCCCEEEECCCCCCEEEEHHHHHHHHCCCCCCCHHHHHHHCCCCCEEEEEEECCCC
DMTLEWQESGVEGANRFLKRVWKLVYEHTTKGEVAALNVAALSEDQKALRRDIHKTIAKV
CCEEEEHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHCHHHHHHHHHHHHHHHHH
TDDIGRRQTFNTAIAAIMELMNKLAKAPQEDEQDRALMQEALLAVVRMLNPFTPHASFTL
HHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
WRELNGEGDIDNAPWPVADESAMVEDSTLVVVQVNGKVRGKITVAVDATEEQVRERAGQE
EEECCCCCCCCCCCCCCCCCCCEEECCEEEEEEECCEEEEEEEEEEECCHHHHHHHCCHH
HLVAKYLDGKTVRKVIYVPGKLLNLVVG
HHHHHHHCCHHEEHEEECCHHHHHHHCC
>Mature Secondary Structure
MKTGPLAAMQEQYRPEEIESKVQLHWDENRTFEVTEDESKEKYYCLSMLPYPSGRLHMGH
CCCCCCHHHHHHCCCHHHCCCEEEEECCCCEEEECCCCCCCCEEEEEECCCCCCCEEECC
VRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTYDNIAYMKNQL
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHH
KMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCPNDQTVLANEQVI
HHEECCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCEEEECCCCEECCCCCEEEECHHHH
DGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKTMQRNWIGRSEGVEI
HHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEE
SFDVNDYADKLTVYTTRPDTFMGCTYLAVAAGHPLAQQAAANNPALAAFIDECRNTKVAE
EEECCCCCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHCCCEECH
ADMATMEKKGVDTGFKAIHPLTGEQIPVWAANFVLMEYGTGAVMAVPGHDQRDYEFASKY
HHHHHHHHCCCCCCHHHHCCCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCCHHHHHHC
GLNIKPVILAADGSEPDLSEQALTEKGVLFNSGEFSGLDYEAGFNAIADKLTAMGVGERK
CCCCEEEEEECCCCCCCCHHHHHHHCCCEEECCCCCCCCHHCCHHHHHHHHHHCCCCCHH
VNYRLRDWGVSRQRYWGAPIPMVTLEDGTVLPTPEDQLPVILPEDVVMDGITSPIKADPE
HCEEEHHCCCCCHHCCCCCCCEEEECCCEECCCCCCCCCEECCHHHHHHCCCCCCCCCCC
WAKTTVNGQPALRETDTFDTFMESSWYYARYTCPQYQEGMLDSKAANYWLPVDIYIGGIE
HHHEECCCCCCCCCCCHHHHHHHCCCEEEEECCCHHHHCCCCCCCCCEEEEEEEEECCHH
HAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQGMVLADAFYYVGENGERNWVSPVDAI
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHEECCCCCCCCCCHHHHH
VERDEKGRIVKAKDAAGHELVYTGMSKMSKSKNNGIDPQVMVERYGADTVRLFMMFASPA
HCCCCCCCEEEECCCCCCEEEEHHHHHHHHCCCCCCCHHHHHHHCCCCCEEEEEEECCCC
DMTLEWQESGVEGANRFLKRVWKLVYEHTTKGEVAALNVAALSEDQKALRRDIHKTIAKV
CCEEEEHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHCHHHHHHHHHHHHHHHHH
TDDIGRRQTFNTAIAAIMELMNKLAKAPQEDEQDRALMQEALLAVVRMLNPFTPHASFTL
HHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
WRELNGEGDIDNAPWPVADESAMVEDSTLVVVQVNGKVRGKITVAVDATEEQVRERAGQE
EEECCCCCCCCCCCCCCCCCCCEEECCEEEEEEECCEEEEEEEEEEECCHHHHHHHCCHH
HLVAKYLDGKTVRKVIYVPGKLLNLVVG
HHHHHHHCCHHEEHEEECCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA