Click here to switch to the map view.

The map label for this gene is actII-2 [H]

Identifier: 238893568

GI number: 238893568

Start: 1417244

End: 1418683

Strand: Reverse

Name: actII-2 [H]

Synonym: KP1_1476

Alternate gene names: 238893568

Gene position: 1418683-1417244 (Counterclockwise)

Preceding gene: 238893572

Following gene: 238893567

Centisome position: 27.03

GC content: 65.9

Gene sequence:

>1440_bases
ATGGAGAATCCCATGAATACGTCTGCTGTTTCACCCGGCCGCGCCGGTCTGTTGCTGCTGCTGAGCGGCCAGATGCTGCC
GCTTATTGATACCTCGATCACCAATGTGGCGCTGGACGCCATCACCCACACCCTGGCGGCCAGCGCCACGCAGCTGGAGC
TTATCGTCGCGCTGTACGGCGTCGCCTTTGCCGTCTGCCTGGCGATGGGCAGTAAGCTTGGCGATAACTATGGCCGCCGG
CGCCTGTTTATGTGGGGCGTCGCCCTCTTTGGCATCGCTTCCCTGCTGTGCGGTATGGCGAACAGCATCGGCGCCCTGCT
GGCGGCCCGCACCCTGCAGGGCGCCGGGGCGGCGCTGATTGTGCCGCAGATCCTCGCGACCCTGCACGTCACCCTCAAAG
GCCCCGCCCACGCCCGGGCCATCAGCCTGTATGGCGGGATCGGCGGGATCGCCTTTATCGTCGGTCAGATGGGCGGCGGC
TGGCTGGTGTCGGCGGACATCGCCGGGCTGGGCTGGCGCAACGCCTTTTTTATCAATGTGCCGATCTGCCTGCTGGTGCT
GGCTCTGAGCCGCCGCTATGTGCCGGAAACCCGTCGCGAGACGCCGTCGCGGATTGACTGGCAGGGTACGCTGTACCTCG
CGCTGATCCTCTGCTGCCTGCTGTTCCCGATGGCCCTCGGTCCGGAGCTGCACTGGCCGCTGTGGCTGCAGCTGATGCTG
GTGGCGGTCCTGCCGCTGCTGTTCGCCATGCGTCAGAGCGCCCTGCGCCAGCAGCAGCGTGGCGACCATCCTCTGCTGCC
GCCGCGTCTGCTGCAGTTGACCAGCATCCGCTTCGGGATGGCCATCGCCCTGCTGTTTTTCAGCGCCTGGTCCGGCTTTA
TGTTCTGCATGGCGCTGACCATGCAGGAGGGGCTGGGGATGGCGCCGTGGCAGTCCGGCAATAGCTTTATCGCCCTCGGC
GTGGCCTATTTTATTTCGGCGCTGTATGCCCCGCGGCTTATCGCCCGCTACAGCATGGGGCGGATCCTGCTGACCGGTCT
GGCGGTGCAGATCGCCGGTCTCCTGCTGCTGTGCGCCACCTTCTCCCGCTTCGGCGTCGCCACCAGCGCCCTGACGCTGG
TCCCCGCCACGGCGCTGATCGGCTATGGCCAGGCGCTGATCGTCAACAGCTTTTATCGGATTGGTATGCGCGATATCAGC
GCCAGCGACGCCGGGGCCGGGAGCGCAATCCTCAGCACCCTGCAGCAGGCGACCCTCGGTCTCGGACCGGCTATCCTTGG
TTCGCTGTTCCTGGCGCTGGCCCGTCGCGGCGGCGGCAACTATCCGCAGGCGCTGATTGATTTTCTGCTGGTCGAGGTGG
CCATGATGCTGTTGCTGGGGGCGATAGCGCTGTGGCTACGCCATCATCTCAACCGACAGCCGGCGACGGTGGCCTCGTAA

Upstream 100 bases:

>100_bases
TCATCACCCCTCCAGCATAGTAGTGCCAATACCAGTATAGACAGGAACTGGTACCCGTTTAATTTATCGGTAATGCTACG
TCGGTCTGCTGAATGACGCA

Downstream 100 bases:

>100_bases
TACCGGCGGCGGTAATTTGCATAATAGCTATCCAGCGGTCATCATACCCGCTGGATATTCAGGAGACGTTATGCAGGAAT
TAATATCGCAAATTGCCGCC

Product: putative major facilitator superfamily (MFS) transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MENPMNTSAVSPGRAGLLLLLSGQMLPLIDTSITNVALDAITHTLAASATQLELIVALYGVAFAVCLAMGSKLGDNYGRR
RLFMWGVALFGIASLLCGMANSIGALLAARTLQGAGAALIVPQILATLHVTLKGPAHARAISLYGGIGGIAFIVGQMGGG
WLVSADIAGLGWRNAFFINVPICLLVLALSRRYVPETRRETPSRIDWQGTLYLALILCCLLFPMALGPELHWPLWLQLML
VAVLPLLFAMRQSALRQQQRGDHPLLPPRLLQLTSIRFGMAIALLFFSAWSGFMFCMALTMQEGLGMAPWQSGNSFIALG
VAYFISALYAPRLIARYSMGRILLTGLAVQIAGLLLLCATFSRFGVATSALTLVPATALIGYGQALIVNSFYRIGMRDIS
ASDAGAGSAILSTLQQATLGLGPAILGSLFLALARRGGGNYPQALIDFLLVEVAMMLLLGAIALWLRHHLNRQPATVAS

Sequences:

>Translated_479_residues
MENPMNTSAVSPGRAGLLLLLSGQMLPLIDTSITNVALDAITHTLAASATQLELIVALYGVAFAVCLAMGSKLGDNYGRR
RLFMWGVALFGIASLLCGMANSIGALLAARTLQGAGAALIVPQILATLHVTLKGPAHARAISLYGGIGGIAFIVGQMGGG
WLVSADIAGLGWRNAFFINVPICLLVLALSRRYVPETRRETPSRIDWQGTLYLALILCCLLFPMALGPELHWPLWLQLML
VAVLPLLFAMRQSALRQQQRGDHPLLPPRLLQLTSIRFGMAIALLFFSAWSGFMFCMALTMQEGLGMAPWQSGNSFIALG
VAYFISALYAPRLIARYSMGRILLTGLAVQIAGLLLLCATFSRFGVATSALTLVPATALIGYGQALIVNSFYRIGMRDIS
ASDAGAGSAILSTLQQATLGLGPAILGSLFLALARRGGGNYPQALIDFLLVEVAMMLLLGAIALWLRHHLNRQPATVAS
>Mature_479_residues
MENPMNTSAVSPGRAGLLLLLSGQMLPLIDTSITNVALDAITHTLAASATQLELIVALYGVAFAVCLAMGSKLGDNYGRR
RLFMWGVALFGIASLLCGMANSIGALLAARTLQGAGAALIVPQILATLHVTLKGPAHARAISLYGGIGGIAFIVGQMGGG
WLVSADIAGLGWRNAFFINVPICLLVLALSRRYVPETRRETPSRIDWQGTLYLALILCCLLFPMALGPELHWPLWLQLML
VAVLPLLFAMRQSALRQQQRGDHPLLPPRLLQLTSIRFGMAIALLFFSAWSGFMFCMALTMQEGLGMAPWQSGNSFIALG
VAYFISALYAPRLIARYSMGRILLTGLAVQIAGLLLLCATFSRFGVATSALTLVPATALIGYGQALIVNSFYRIGMRDIS
ASDAGAGSAILSTLQQATLGLGPAILGSLFLALARRGGGNYPQALIDFLLVEVAMMLLLGAIALWLRHHLNRQPATVAS

Specific function: Promotes the efflux of actinorhodin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=404, Percent_Identity=25.4950495049505, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI87081983, Length=351, Percent_Identity=27.6353276353276, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1788392, Length=419, Percent_Identity=23.3890214797136, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1789042, Length=165, Percent_Identity=30.3030303030303, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1788710, Length=314, Percent_Identity=26.1146496815287, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790146, Length=171, Percent_Identity=30.9941520467836, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50974; Mature: 50974

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENPMNTSAVSPGRAGLLLLLSGQMLPLIDTSITNVALDAITHTLAASATQLELIVALYG
CCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAFAVCLAMGSKLGDNYGRRRLFMWGVALFGIASLLCGMANSIGALLAARTLQGAGAALI
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
VPQILATLHVTLKGPAHARAISLYGGIGGIAFIVGQMGGGWLVSADIAGLGWRNAFFINV
HHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEEH
PICLLVLALSRRYVPETRRETPSRIDWQGTLYLALILCCLLFPMALGPELHWPLWLQLML
HHHHHHHHHHHHCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
VAVLPLLFAMRQSALRQQQRGDHPLLPPRLLQLTSIRFGMAIALLFFSAWSGFMFCMALT
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MQEGLGMAPWQSGNSFIALGVAYFISALYAPRLIARYSMGRILLTGLAVQIAGLLLLCAT
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSRFGVATSALTLVPATALIGYGQALIVNSFYRIGMRDISASDAGAGSAILSTLQQATLG
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHC
LGPAILGSLFLALARRGGGNYPQALIDFLLVEVAMMLLLGAIALWLRHHLNRQPATVAS
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MENPMNTSAVSPGRAGLLLLLSGQMLPLIDTSITNVALDAITHTLAASATQLELIVALYG
CCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAFAVCLAMGSKLGDNYGRRRLFMWGVALFGIASLLCGMANSIGALLAARTLQGAGAALI
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
VPQILATLHVTLKGPAHARAISLYGGIGGIAFIVGQMGGGWLVSADIAGLGWRNAFFINV
HHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEEH
PICLLVLALSRRYVPETRRETPSRIDWQGTLYLALILCCLLFPMALGPELHWPLWLQLML
HHHHHHHHHHHHCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
VAVLPLLFAMRQSALRQQQRGDHPLLPPRLLQLTSIRFGMAIALLFFSAWSGFMFCMALT
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MQEGLGMAPWQSGNSFIALGVAYFISALYAPRLIARYSMGRILLTGLAVQIAGLLLLCAT
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSRFGVATSALTLVPATALIGYGQALIVNSFYRIGMRDISASDAGAGSAILSTLQQATLG
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHC
LGPAILGSLFLALARRGGGNYPQALIDFLLVEVAMMLLLGAIALWLRHHLNRQPATVAS
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1878971; 12000953 [H]