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The map label for this gene is kstD [H]

Identifier: 238893371

GI number: 238893371

Start: 1211012

End: 1212688

Strand: Reverse

Name: kstD [H]

Synonym: KP1_1254

Alternate gene names: 238893371

Gene position: 1212688-1211012 (Counterclockwise)

Preceding gene: 238893372

Following gene: 238893370

Centisome position: 23.11

GC content: 64.94

Gene sequence:

>1677_bases
ATGAAGAGCTGGGATGTGATCGTCATCGGCAGCGGCGCGGCCGGTTTTGCCGCCGCGGTCACCGCCTGCTGTAAAGGGCT
GTCGGTGCTGATGCTGGAGAAAGCCGGCCAGTTCGGCGGCACCTCGGCCATCTCCGGCGGCGCCGTGTGGCTGCACGATA
CCGACCAGGCGCGCGCCGAGGGCAAAAGCGGTAGCGCCGAGGCGATGAAAACCTACCTGCGAACCATTATCGGCGAGGGC
CAGTACCGAGAAGATCTTGCCGAAGCGTTTGTCAGCGCCGGGCGGGAGGCGCTGGCCTTTCTCGAGCGTGAGGGGGCAGT
AAAGTACAGCCTGCGTCCGCTCTCGCCCGATTACTATCCGGATGAACCCGGCGCAGTCGACGTCGGTCGTGCGCTGGAGG
TGGTGGAGTATGACGGTCGTGAACTGGGGGACGCGTTTCGCGACCTGCGTTCACCGCCGCCGGGGATGCTGCTGTTTGGC
GGGATGATGGTCAACCGCGTCGATATTCAACATTTTCTCGACATGCGCCGCTCGCTGCGCTCCCTGGCCCATTGCACCCG
GCTGCTGCTGCGCTATGCCCGCGACCGGGTGAAGTACCCTCGCGGTACCCGCCTGGCGATGGGCAATGCGCTGATAGCCC
GCATGGCGACAACGGCGCTGCGTAAAGGCATGAACCTGCGGCTGAATGTTAATGTTCTGGCGCTGTGCGAAGCGCAAGGC
GCGGTCCACGGGGTGGAGATTGAGTATCAGGGGCAAAGAGAGACGCTGCACGCCCGGCGCGGGGTGGTGCTGGCCGCAGG
CGGCTTCGCCGCGGGCGCGCTGGCGGCGCGCTATCGGCCGCACACCCGCGAGCATTTCACCATGTCGCCGCCGGCCAACG
ATGGCGCCGCGCTGCGTCTCGCGGCGGCGCTTAACGCTCGCGAGGGAGCCGATCGGCCATCCAATTTTTTCTGGGCGCCG
GTATCGGTGCTGACCCGGACCGACGGCAGCGAGGAGCGCTTCCCCCATCTGGTAACCGATCGCGCAAAACCGGGCGTGAT
TGCCGTCAATCAGCGGGCGGTACGCTTCGTCAACGAGTCCAGCTCCTATCACCATTTCGCCAGCGCAATGCAGGATGCAG
CGGAAAATGCCCCCTGTTTTTTACTCTGCGACGCCCAGGCGATGAAGCGTTATGGCCTCGGACTGGCGCGCCCGGCGCCG
GTCAATAATGACGCCCTGGTCGCCGCTGGCTATCTGCACAAAGCCGATACCCTGGCCGCGCTGGCGCAACAGCTTGGTCT
GGATGCCCAAACCCTGAGCAAAACGGTAGCCCGCTACAACCGCGACGCCGCGCAGGGCGTGGATCGGGAATTTGCTAAAG
GCGGCAACAGCTATAACCGGGCGATGGGCGATCCGGGCCATCAGCCTGACGCCTGTAATGCCCCCCTGCTCAGCGCGCCG
TTCTATGCCATCAAACTTTATACCGGCGATCTGGGGACCTCACGGGGCCTGGTCACCACCGCCGATGCCCAGGTCGTCAA
TACCGAAGGACACCCCATCCCGGGGCTGTACGCGGTGGGAAACGACATGGATTCGCTGATGGCCGGCACCTATCCGGGCC
CCGGCATCACCCTCGGCCCGGGACTGACGTTTGGCTATCTCGCCGCCTGCCATCTGGCACAACACTCCACCCACTAA

Upstream 100 bases:

>100_bases
GTGGTTGCAAAGTACAGACCGGGCCGGAAATGGCGCTGGCGCAGATGAGGCTGATGGGCCAGTTTATCGGCGCCATCCCG
CAGGCGCAGGGAGCGGCGGC

Downstream 100 bases:

>100_bases
TCAGGAAAACATCATGATCTACGAAAAACGCACGTACACCATCAATCCGCTGAAAATGGCCGACTGGCTGGCGCTGTACC
AAAGCGATGCCCTGGCGGTG

Product: putative electron transfer flavoprotein-NAD/FAD/quinone oxidoreductase

Products: NA

Alternate protein names: 3-keto-Delta(4)-steroid Delta(1)-dehydrogenase; KSDD; 3-oxo-Delta(4)-steroid 1-dehydrogenase; KSTD [H]

Number of amino acids: Translated: 558; Mature: 558

Protein sequence:

>558_residues
MKSWDVIVIGSGAAGFAAAVTACCKGLSVLMLEKAGQFGGTSAISGGAVWLHDTDQARAEGKSGSAEAMKTYLRTIIGEG
QYREDLAEAFVSAGREALAFLEREGAVKYSLRPLSPDYYPDEPGAVDVGRALEVVEYDGRELGDAFRDLRSPPPGMLLFG
GMMVNRVDIQHFLDMRRSLRSLAHCTRLLLRYARDRVKYPRGTRLAMGNALIARMATTALRKGMNLRLNVNVLALCEAQG
AVHGVEIEYQGQRETLHARRGVVLAAGGFAAGALAARYRPHTREHFTMSPPANDGAALRLAAALNAREGADRPSNFFWAP
VSVLTRTDGSEERFPHLVTDRAKPGVIAVNQRAVRFVNESSSYHHFASAMQDAAENAPCFLLCDAQAMKRYGLGLARPAP
VNNDALVAAGYLHKADTLAALAQQLGLDAQTLSKTVARYNRDAAQGVDREFAKGGNSYNRAMGDPGHQPDACNAPLLSAP
FYAIKLYTGDLGTSRGLVTTADAQVVNTEGHPIPGLYAVGNDMDSLMAGTYPGPGITLGPGLTFGYLAACHLAQHSTH

Sequences:

>Translated_558_residues
MKSWDVIVIGSGAAGFAAAVTACCKGLSVLMLEKAGQFGGTSAISGGAVWLHDTDQARAEGKSGSAEAMKTYLRTIIGEG
QYREDLAEAFVSAGREALAFLEREGAVKYSLRPLSPDYYPDEPGAVDVGRALEVVEYDGRELGDAFRDLRSPPPGMLLFG
GMMVNRVDIQHFLDMRRSLRSLAHCTRLLLRYARDRVKYPRGTRLAMGNALIARMATTALRKGMNLRLNVNVLALCEAQG
AVHGVEIEYQGQRETLHARRGVVLAAGGFAAGALAARYRPHTREHFTMSPPANDGAALRLAAALNAREGADRPSNFFWAP
VSVLTRTDGSEERFPHLVTDRAKPGVIAVNQRAVRFVNESSSYHHFASAMQDAAENAPCFLLCDAQAMKRYGLGLARPAP
VNNDALVAAGYLHKADTLAALAQQLGLDAQTLSKTVARYNRDAAQGVDREFAKGGNSYNRAMGDPGHQPDACNAPLLSAP
FYAIKLYTGDLGTSRGLVTTADAQVVNTEGHPIPGLYAVGNDMDSLMAGTYPGPGITLGPGLTFGYLAACHLAQHSTH
>Mature_558_residues
MKSWDVIVIGSGAAGFAAAVTACCKGLSVLMLEKAGQFGGTSAISGGAVWLHDTDQARAEGKSGSAEAMKTYLRTIIGEG
QYREDLAEAFVSAGREALAFLEREGAVKYSLRPLSPDYYPDEPGAVDVGRALEVVEYDGRELGDAFRDLRSPPPGMLLFG
GMMVNRVDIQHFLDMRRSLRSLAHCTRLLLRYARDRVKYPRGTRLAMGNALIARMATTALRKGMNLRLNVNVLALCEAQG
AVHGVEIEYQGQRETLHARRGVVLAAGGFAAGALAARYRPHTREHFTMSPPANDGAALRLAAALNAREGADRPSNFFWAP
VSVLTRTDGSEERFPHLVTDRAKPGVIAVNQRAVRFVNESSSYHHFASAMQDAAENAPCFLLCDAQAMKRYGLGLARPAP
VNNDALVAAGYLHKADTLAALAQQLGLDAQTLSKTVARYNRDAAQGVDREFAKGGNSYNRAMGDPGHQPDACNAPLLSAP
FYAIKLYTGDLGTSRGLVTTADAQVVNTEGHPIPGLYAVGNDMDSLMAGTYPGPGITLGPGLTFGYLAACHLAQHSTH

Specific function: Catalyzes the elimination of the C-1 and C-2 hydrogen atoms of the A-ring from the polycyclic ring structure of 3- ketosteroids. Is also involved in the formation of 3-keto-1,4- diene-steroid from 3-keto-4-ene-steroid [H]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. 3-oxosteroid dehydrogenase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.3.99.4 [H]

Molecular weight: Translated: 59689; Mature: 59689

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSWDVIVIGSGAAGFAAAVTACCKGLSVLMLEKAGQFGGTSAISGGAVWLHDTDQARAE
CCCCEEEEECCCCCHHHHHHHHHHCCHHEEHHHHCCCCCCCCCCCCCEEEEECCHHHHHC
GKSGSAEAMKTYLRTIIGEGQYREDLAEAFVSAGREALAFLEREGAVKYSLRPLSPDYYP
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEECCCCCCCC
DEPGAVDVGRALEVVEYDGRELGDAFRDLRSPPPGMLLFGGMMVNRVDIQHFLDMRRSLR
CCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHH
SLAHCTRLLLRYARDRVKYPRGTRLAMGNALIARMATTALRKGMNLRLNVNVLALCEAQG
HHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCCCEEEECEEEEEEECCC
AVHGVEIEYQGQRETLHARRGVVLAAGGFAAGALAARYRPHTREHFTMSPPANDGAALRL
CEEEEEEEECCCHHHHHHHCCEEEEECCHHHHHHHHHCCCCCCCCEEECCCCCCCCCEEE
AAALNAREGADRPSNFFWAPVSVLTRTDGSEERFPHLVTDRAKPGVIAVNQRAVRFVNES
EEHHCCCCCCCCCCCEEEEHHHHEECCCCCHHHCCCHHCCCCCCCEEEECHHHHHHHCCC
SSYHHFASAMQDAAENAPCFLLCDAQAMKRYGLGLARPAPVNNDALVAAGYLHKADTLAA
CCHHHHHHHHHHHHCCCCEEEEECHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHH
LAQQLGLDAQTLSKTVARYNRDAAQGVDREFAKGGNSYNRAMGDPGHQPDACNAPLLSAP
HHHHHCCCHHHHHHHHHHHCCHHHHCCHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCCC
FYAIKLYTGDLGTSRGLVTTADAQVVNTEGHPIPGLYAVGNDMDSLMAGTYPGPGITLGP
EEEEEEEECCCCCCCCEEEECCCEEEECCCCCCCCEEEECCCHHHHHHCCCCCCCEEECC
GLTFGYLAACHLAQHSTH
CCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKSWDVIVIGSGAAGFAAAVTACCKGLSVLMLEKAGQFGGTSAISGGAVWLHDTDQARAE
CCCCEEEEECCCCCHHHHHHHHHHCCHHEEHHHHCCCCCCCCCCCCCEEEEECCHHHHHC
GKSGSAEAMKTYLRTIIGEGQYREDLAEAFVSAGREALAFLEREGAVKYSLRPLSPDYYP
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEECCCCCCCC
DEPGAVDVGRALEVVEYDGRELGDAFRDLRSPPPGMLLFGGMMVNRVDIQHFLDMRRSLR
CCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHH
SLAHCTRLLLRYARDRVKYPRGTRLAMGNALIARMATTALRKGMNLRLNVNVLALCEAQG
HHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCCCEEEECEEEEEEECCC
AVHGVEIEYQGQRETLHARRGVVLAAGGFAAGALAARYRPHTREHFTMSPPANDGAALRL
CEEEEEEEECCCHHHHHHHCCEEEEECCHHHHHHHHHCCCCCCCCEEECCCCCCCCCEEE
AAALNAREGADRPSNFFWAPVSVLTRTDGSEERFPHLVTDRAKPGVIAVNQRAVRFVNES
EEHHCCCCCCCCCCCEEEEHHHHEECCCCCHHHCCCHHCCCCCCCEEEECHHHHHHHCCC
SSYHHFASAMQDAAENAPCFLLCDAQAMKRYGLGLARPAPVNNDALVAAGYLHKADTLAA
CCHHHHHHHHHHHHCCCCEEEEECHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHH
LAQQLGLDAQTLSKTVARYNRDAAQGVDREFAKGGNSYNRAMGDPGHQPDACNAPLLSAP
HHHHHCCCHHHHHHHHHHHCCHHHHCCHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCCC
FYAIKLYTGDLGTSRGLVTTADAQVVNTEGHPIPGLYAVGNDMDSLMAGTYPGPGITLGP
EEEEEEEECCCCCCCCEEEECCCEEEECCCCCCCCEEEECCCHHHHHHCCCCCCCEEECC
GLTFGYLAACHLAQHSTH
CCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036 [H]