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The map label for this gene is sbcD [H]

Identifier: 238893322

GI number: 238893322

Start: 1163387

End: 1164592

Strand: Reverse

Name: sbcD [H]

Synonym: KP1_1199

Alternate gene names: 238893322

Gene position: 1164592-1163387 (Counterclockwise)

Preceding gene: 238893328

Following gene: 238893321

Centisome position: 22.19

GC content: 62.52

Gene sequence:

>1206_bases
ATGCGCCTCCTTCACACCTCCGACTGGCACCTGGGCCAGAATTTTTACAGCAAAAGCCGCGCGGCCGAACACGACGCTTT
TCTTACCTGGCTACTGGACCGCGCGCAGGAGCATGAAGTCGACGCCATTATCGTGGCTGGCGATATTTTTGATACCGGCT
CGCCGCCGAGCTACGCCCGCGAACTGTATAACCGTTTCGTCGTCCAGTTGCAGCAGACCGGCTGTCGCCTGGTGGTGCTG
GCGGGCAACCACGACTCGGTGGCGATGCTCAATGAATCCCGCGATATCCTCGCCTTTTTACACACCACCGTGGTGGCGAA
CGCCGGCTACGCGCCGATTGAGCTGCCGCTGCGCGACGGCACGCCGGGCGCCATCTTCTGTCCGGTGCCGTTTTTACGCC
CGCGCGAGCTGGTGACCAGCCAGGCCGGTCACTCCGGTCGGGAAAAACAGCAGCTGCTGCTGCACGCCATCAGCGACTAT
TATCAGGAGCAGTATCAGCAGGCCTGCGCGCTGCGCGGCGATCGGCCGCTGCCCATCATTGCCAGCGGCCATCTGACCAC
CGTCGGCGCCAGCAAAAGCGATGCCGTCCGCGACATTTACATCGGCACACTGGATGCCTTTCCGGCGCAACACTTCCCCC
CTGCCGACTACATTGCGCTGGGCCATATTCACCGGGCGCAGATGGTCGGCGGCTGCGAGCATATCCGCTACAGCGGCTCG
CCGCTGCCGCTGAGCTTCGATGAAACCGGCAAAGCGAAATCGGTGCATCTGGTGAGCTTTAGCGAGGGGCGACTTAGCGC
CGTGGAAACCCTGGAGGTGCCCGTGACCCAGCCTCTGGCGGTAATCAAAGGCGATCTGGCCGCCATCACCGCCCAGCTGG
AGCAGTGGCGCGGCGTCGAGCAGGACCCGCCGGTGTGGCTGGATATCGAAATCACCACCGAGGACTATCTGCACGATATT
CAGCGTCATATTCAGGCGCTGACGGAGGATCTGCCGGTGGAAGTGCTGCTGGTGCGCCGCAGCCGCGAGCAGCGCGAGAA
AATACTGCTCAACGCCCAGCGGGAGACGCTCAGTGAACTGAAGGTGGAGGAGGTATTCGAGCGCCGGCTGGCGCTGACCG
AAATTGACGATATGAAGCGCGCCCGGCTGCATGAGCTGTTCGCCCATACGGTGCATACGCTCACCGCGGAGGATGAAAAC
GCATGA

Upstream 100 bases:

>100_bases
TGACCGTCTGATGATCGGGTATTTTGAAGCACGACGCGGACTTACCCCTCACTAACAAGTGGTATACTGCCGCCAATGCT
TTTTTCGCTAGGGAAGATTT

Downstream 100 bases:

>100_bases
AAATCCTCAGTCTGCGTCTGAAAAACCTCAACTCCCTGAAAGGGGAATGGAAGATAGACTTTACCGCCGAGCCGTTCGCC
AGCAATGGCCTGTTCGCCAT

Product: exonuclease subunit SbcD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MRLLHTSDWHLGQNFYSKSRAAEHDAFLTWLLDRAQEHEVDAIIVAGDIFDTGSPPSYARELYNRFVVQLQQTGCRLVVL
AGNHDSVAMLNESRDILAFLHTTVVANAGYAPIELPLRDGTPGAIFCPVPFLRPRELVTSQAGHSGREKQQLLLHAISDY
YQEQYQQACALRGDRPLPIIASGHLTTVGASKSDAVRDIYIGTLDAFPAQHFPPADYIALGHIHRAQMVGGCEHIRYSGS
PLPLSFDETGKAKSVHLVSFSEGRLSAVETLEVPVTQPLAVIKGDLAAITAQLEQWRGVEQDPPVWLDIEITTEDYLHDI
QRHIQALTEDLPVEVLLVRRSREQREKILLNAQRETLSELKVEEVFERRLALTEIDDMKRARLHELFAHTVHTLTAEDEN
A

Sequences:

>Translated_401_residues
MRLLHTSDWHLGQNFYSKSRAAEHDAFLTWLLDRAQEHEVDAIIVAGDIFDTGSPPSYARELYNRFVVQLQQTGCRLVVL
AGNHDSVAMLNESRDILAFLHTTVVANAGYAPIELPLRDGTPGAIFCPVPFLRPRELVTSQAGHSGREKQQLLLHAISDY
YQEQYQQACALRGDRPLPIIASGHLTTVGASKSDAVRDIYIGTLDAFPAQHFPPADYIALGHIHRAQMVGGCEHIRYSGS
PLPLSFDETGKAKSVHLVSFSEGRLSAVETLEVPVTQPLAVIKGDLAAITAQLEQWRGVEQDPPVWLDIEITTEDYLHDI
QRHIQALTEDLPVEVLLVRRSREQREKILLNAQRETLSELKVEEVFERRLALTEIDDMKRARLHELFAHTVHTLTAEDEN
A
>Mature_401_residues
MRLLHTSDWHLGQNFYSKSRAAEHDAFLTWLLDRAQEHEVDAIIVAGDIFDTGSPPSYARELYNRFVVQLQQTGCRLVVL
AGNHDSVAMLNESRDILAFLHTTVVANAGYAPIELPLRDGTPGAIFCPVPFLRPRELVTSQAGHSGREKQQLLLHAISDY
YQEQYQQACALRGDRPLPIIASGHLTTVGASKSDAVRDIYIGTLDAFPAQHFPPADYIALGHIHRAQMVGGCEHIRYSGS
PLPLSFDETGKAKSVHLVSFSEGRLSAVETLEVPVTQPLAVIKGDLAAITAQLEQWRGVEQDPPVWLDIEITTEDYLHDI
QRHIQALTEDLPVEVLLVRRSREQREKILLNAQRETLSELKVEEVFERRLALTEIDDMKRARLHELFAHTVHTLTAEDEN
A

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=399, Percent_Identity=77.9448621553885, Blast_Score=655, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 44946; Mature: 44946

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLHTSDWHLGQNFYSKSRAAEHDAFLTWLLDRAQEHEVDAIIVAGDIFDTGSPPSYAR
CCCEECCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHH
ELYNRFVVQLQQTGCRLVVLAGNHDSVAMLNESRDILAFLHTTVVANAGYAPIELPLRDG
HHHHHHHHHHHCCCCEEEEEECCCCCEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCC
TPGAIFCPVPFLRPRELVTSQAGHSGREKQQLLLHAISDYYQEQYQQACALRGDRPLPII
CCCEEECCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
ASGHLTTVGASKSDAVRDIYIGTLDAFPAQHFPPADYIALGHIHRAQMVGGCEHIRYSGS
ECCCEEEECCCCCCCHHEEEEEECCCCCCCCCCCCCCEECCCHHHHHHHCCHHHEEECCC
PLPLSFDETGKAKSVHLVSFSEGRLSAVETLEVPVTQPLAVIKGDLAAITAQLEQWRGVE
CCCCCCCCCCCCCEEEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCC
QDPPVWLDIEITTEDYLHDIQRHIQALTEDLPVEVLLVRRSREQREKILLNAQRETLSEL
CCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHH
KVEEVFERRLALTEIDDMKRARLHELFAHTVHTLTAEDENA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCC
>Mature Secondary Structure
MRLLHTSDWHLGQNFYSKSRAAEHDAFLTWLLDRAQEHEVDAIIVAGDIFDTGSPPSYAR
CCCEECCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHH
ELYNRFVVQLQQTGCRLVVLAGNHDSVAMLNESRDILAFLHTTVVANAGYAPIELPLRDG
HHHHHHHHHHHCCCCEEEEEECCCCCEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCC
TPGAIFCPVPFLRPRELVTSQAGHSGREKQQLLLHAISDYYQEQYQQACALRGDRPLPII
CCCEEECCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
ASGHLTTVGASKSDAVRDIYIGTLDAFPAQHFPPADYIALGHIHRAQMVGGCEHIRYSGS
ECCCEEEECCCCCCCHHEEEEEECCCCCCCCCCCCCCEECCCHHHHHHHCCHHHEEECCC
PLPLSFDETGKAKSVHLVSFSEGRLSAVETLEVPVTQPLAVIKGDLAAITAQLEQWRGVE
CCCCCCCCCCCCCEEEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCC
QDPPVWLDIEITTEDYLHDIQRHIQALTEDLPVEVLLVRRSREQREKILLNAQRETLSEL
CCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHH
KVEEVFERRLALTEIDDMKRARLHELFAHTVHTLTAEDENA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]