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The map label for this gene is acoC [H]

Identifier: 238893247

GI number: 238893247

Start: 1089708

End: 1091243

Strand: Reverse

Name: acoC [H]

Synonym: KP1_1117

Alternate gene names: 238893247

Gene position: 1091243-1089708 (Counterclockwise)

Preceding gene: 238893248

Following gene: 238893246

Centisome position: 20.79

GC content: 61.07

Gene sequence:

>1536_bases
ATGAGCGAAATCAAGACGCTTGAAATGCCAAAGTGGGGGCTTTCCATGGAGGAAGGCTTGCTCGCTCGGTGGGCAATCCA
GGAGGGTGACAGCTTCACCCCAGGGCAGGAAATCTGTGAGATTGAAACCAGTAAAATCGTCAATGTGCTGGAGGCCCCCT
TTGCCGGTACGTTACGTCGGATACTCGCCCGCGAGGGTGAGACGCTTCAGGTAGGCGCCGTGCTGGCCCTGGCGGCTGAC
GCGTCGGTCAGCGATGCTGAACTGGACGAATTTGCTGCCAGCCTGGCGACGGTGAAACCCGCAGCCCCAGGCCCGGAGGC
TGCCGCGCCGGACGTAGCGGCACAGGCAGGCGCTAAGCCAGCTTCCGTTGTTTCCCCGCCATCCAACAGCCCCGAGCCCC
CTGTTGGGCAGACCGTCATCCCCGTCAGTCTGCAAGGCGTGACCGATGTGACTCAGGTTAATGCCACGCCCCATGCGTTA
CGACTGTCTGCCCGCTGGGGTGTCGACCTGAAAAAAGTCCGCGGCAGCGGGCGCGGGGATCGTATCTCTGTATCTGATCT
GGAAAGCGCGATCGTTGCCGCCGGCGGTCGCCTGGCCTCTCCGACGCCTCCCGTTCGTCGCAGCAAAGCGCCCCGCTCGC
ATGCCGATGATAGCCAGGTATCGGCCACCCCGCTGGCGCGCCGTCTGGCCGGCAAACTGGGTATCAACTTACATGACTGC
CGAAGCAGCGGTTCACGCGGTCGGGTGAGTCGCGATGATGTGCTGGCCGCGGCGTTGTTACTCGACGAGCAACCGCAGAC
CAGCCCTGTGCAGGAGAGTGCCCCGGCACCCTTTGAGAGCATCCCGATGTCCGGTATGCGGCGGGCTATCGCTTCTCGCC
TGCAAACGTCCAAGCAACAGTCTCCCCATTTCCGTTTGAGCGTCGATCTCGATCTGGAGCGACTGTTAGCGTTACGTCAG
GACATTAACCGCGAAGTACCCGGCGTCAAGATTTCGGTGAATGACCTGCTGGTGAAGGCCTGCGCCCTGGCGCTGGTGGC
CGTGCCTGACGTCAATATCCAGTTTGATGAAGCGGCACAAAGTATCCGCCGCTTTACGGATGCCGACATTTCGGTGGCCG
TTGCGCTGCCTGCCGGGCTAATTACCCCTATTGTTCGCTCGGCGAACCGGAAATCGATCAGCGACATATCGAACGAAATT
CACTCGCTGGTGACCAGAGCCAAAGCGGGCACGCTGAAGCCGGAAGAGTTCCAGGGCGGGACCTTTAGCCTGTCAAATCT
GGGGATGCTCGGCGTCCGGCAGTTTGACGCCATCATCAATCCACCGCAAAGCGCAATCCTCGCCATTGGCGCCGGCGAGG
TCCGGGCGGTAGTACGCGACGGGCAAATCGTCGCGCGCCAGCAAATGACGGTATCGCTATCCTGCGATCACCGGGTCATC
GATGGCGCGGCAGGTGCGGCTTTTCTCCGGGAACTCAAGCGACTGATTGAAACCCCGACCTTGATGTTTATCCAGGAGAC
GAGCTATGCACGATAA

Upstream 100 bases:

>100_bases
ACACACGCCGGTCCCGTTCTCCCCTGCTCTCGAAAAACTCTGGATCCCTGGCGTAGAACGTATCGAAGCCGCCGTCCGTC
AAGTGCTGGAGGATTAACCT

Downstream 100 bases:

>100_bases
ATACGATGTGCTGATCATCGGCGGGGGTCCTGGGGGATACGTTGCCGCCATCCGTGCCGGCCAGCTAGGGCTTCGTACCG
TATTAGTGGAAAAACAACAT

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MSEIKTLEMPKWGLSMEEGLLARWAIQEGDSFTPGQEICEIETSKIVNVLEAPFAGTLRRILAREGETLQVGAVLALAAD
ASVSDAELDEFAASLATVKPAAPGPEAAAPDVAAQAGAKPASVVSPPSNSPEPPVGQTVIPVSLQGVTDVTQVNATPHAL
RLSARWGVDLKKVRGSGRGDRISVSDLESAIVAAGGRLASPTPPVRRSKAPRSHADDSQVSATPLARRLAGKLGINLHDC
RSSGSRGRVSRDDVLAAALLLDEQPQTSPVQESAPAPFESIPMSGMRRAIASRLQTSKQQSPHFRLSVDLDLERLLALRQ
DINREVPGVKISVNDLLVKACALALVAVPDVNIQFDEAAQSIRRFTDADISVAVALPAGLITPIVRSANRKSISDISNEI
HSLVTRAKAGTLKPEEFQGGTFSLSNLGMLGVRQFDAIINPPQSAILAIGAGEVRAVVRDGQIVARQQMTVSLSCDHRVI
DGAAGAAFLRELKRLIETPTLMFIQETSYAR

Sequences:

>Translated_511_residues
MSEIKTLEMPKWGLSMEEGLLARWAIQEGDSFTPGQEICEIETSKIVNVLEAPFAGTLRRILAREGETLQVGAVLALAAD
ASVSDAELDEFAASLATVKPAAPGPEAAAPDVAAQAGAKPASVVSPPSNSPEPPVGQTVIPVSLQGVTDVTQVNATPHAL
RLSARWGVDLKKVRGSGRGDRISVSDLESAIVAAGGRLASPTPPVRRSKAPRSHADDSQVSATPLARRLAGKLGINLHDC
RSSGSRGRVSRDDVLAAALLLDEQPQTSPVQESAPAPFESIPMSGMRRAIASRLQTSKQQSPHFRLSVDLDLERLLALRQ
DINREVPGVKISVNDLLVKACALALVAVPDVNIQFDEAAQSIRRFTDADISVAVALPAGLITPIVRSANRKSISDISNEI
HSLVTRAKAGTLKPEEFQGGTFSLSNLGMLGVRQFDAIINPPQSAILAIGAGEVRAVVRDGQIVARQQMTVSLSCDHRVI
DGAAGAAFLRELKRLIETPTLMFIQETSYAR
>Mature_510_residues
SEIKTLEMPKWGLSMEEGLLARWAIQEGDSFTPGQEICEIETSKIVNVLEAPFAGTLRRILAREGETLQVGAVLALAADA
SVSDAELDEFAASLATVKPAAPGPEAAAPDVAAQAGAKPASVVSPPSNSPEPPVGQTVIPVSLQGVTDVTQVNATPHALR
LSARWGVDLKKVRGSGRGDRISVSDLESAIVAAGGRLASPTPPVRRSKAPRSHADDSQVSATPLARRLAGKLGINLHDCR
SSGSRGRVSRDDVLAAALLLDEQPQTSPVQESAPAPFESIPMSGMRRAIASRLQTSKQQSPHFRLSVDLDLERLLALRQD
INREVPGVKISVNDLLVKACALALVAVPDVNIQFDEAAQSIRRFTDADISVAVALPAGLITPIVRSANRKSISDISNEIH
SLVTRAKAGTLKPEEFQGGTFSLSNLGMLGVRQFDAIINPPQSAILAIGAGEVRAVVRDGQIVARQQMTVSLSCDHRVID
GAAGAAFLRELKRLIETPTLMFIQETSYAR

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=299, Percent_Identity=43.4782608695652, Blast_Score=241, Evalue=1e-63,
Organism=Homo sapiens, GI203098816, Length=242, Percent_Identity=41.3223140495868, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI203098753, Length=242, Percent_Identity=41.3223140495868, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI110671329, Length=330, Percent_Identity=30.3030303030303, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI260898739, Length=162, Percent_Identity=41.358024691358, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI19923748, Length=225, Percent_Identity=33.7777777777778, Blast_Score=126, Evalue=4e-29,
Organism=Escherichia coli, GI1786305, Length=307, Percent_Identity=31.5960912052117, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1786946, Length=310, Percent_Identity=29.3548387096774, Blast_Score=136, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17560088, Length=319, Percent_Identity=40.4388714733542, Blast_Score=220, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI17538894, Length=226, Percent_Identity=42.4778761061947, Blast_Score=167, Evalue=8e-42,
Organism=Caenorhabditis elegans, GI17537937, Length=327, Percent_Identity=30.2752293577982, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI25146366, Length=223, Percent_Identity=35.8744394618834, Blast_Score=137, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6324258, Length=328, Percent_Identity=39.3292682926829, Blast_Score=207, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6320352, Length=246, Percent_Identity=37.8048780487805, Blast_Score=155, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24582497, Length=292, Percent_Identity=39.041095890411, Blast_Score=203, Evalue=2e-52,
Organism=Drosophila melanogaster, GI20129315, Length=292, Percent_Identity=39.041095890411, Blast_Score=203, Evalue=2e-52,
Organism=Drosophila melanogaster, GI18859875, Length=307, Percent_Identity=32.5732899022801, Blast_Score=142, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24645909, Length=227, Percent_Identity=32.5991189427313, Blast_Score=123, Evalue=2e-28,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 54126; Mature: 53995

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEIKTLEMPKWGLSMEEGLLARWAIQEGDSFTPGQEICEIETSKIVNVLEAPFAGTLRR
CCCCCCCCCCCCCCCHHHCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHH
ILAREGETLQVGAVLALAADASVSDAELDEFAASLATVKPAAPGPEAAAPDVAAQAGAKP
HHHCCCCEEEECEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCC
ASVVSPPSNSPEPPVGQTVIPVSLQGVTDVTQVNATPHALRLSARWGVDLKKVRGSGRGD
CCEECCCCCCCCCCCCCEEEEEEECCCCCHHHCCCCCCEEEEECCCCCCHHHHCCCCCCC
RISVSDLESAIVAAGGRLASPTPPVRRSKAPRSHADDSQVSATPLARRLAGKLGINLHDC
EEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHH
RSSGSRGRVSRDDVLAAALLLDEQPQTSPVQESAPAPFESIPMSGMRRAIASRLQTSKQQ
HCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHCC
SPHFRLSVDLDLERLLALRQDINREVPGVKISVNDLLVKACALALVAVPDVNIQFDEAAQ
CCCEEEEECCCHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCEEHHHHHH
SIRRFTDADISVAVALPAGLITPIVRSANRKSISDISNEIHSLVTRAKAGTLKPEEFQGG
HHHHHCCCCCEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCC
TFSLSNLGMLGVRQFDAIINPPQSAILAIGAGEVRAVVRDGQIVARQQMTVSLSCDHRVI
EEECCCCCHHHHHHHHHHCCCCCCEEEEECCCCEEEEEECCCEEEEEEEEEEECCCCEEE
DGAAGAAFLRELKRLIETPTLMFIQETSYAR
CCCHHHHHHHHHHHHHCCCCEEEEECCCCCC
>Mature Secondary Structure 
SEIKTLEMPKWGLSMEEGLLARWAIQEGDSFTPGQEICEIETSKIVNVLEAPFAGTLRR
CCCCCCCCCCCCCCHHHCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHH
ILAREGETLQVGAVLALAADASVSDAELDEFAASLATVKPAAPGPEAAAPDVAAQAGAKP
HHHCCCCEEEECEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCC
ASVVSPPSNSPEPPVGQTVIPVSLQGVTDVTQVNATPHALRLSARWGVDLKKVRGSGRGD
CCEECCCCCCCCCCCCCEEEEEEECCCCCHHHCCCCCCEEEEECCCCCCHHHHCCCCCCC
RISVSDLESAIVAAGGRLASPTPPVRRSKAPRSHADDSQVSATPLARRLAGKLGINLHDC
EEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHH
RSSGSRGRVSRDDVLAAALLLDEQPQTSPVQESAPAPFESIPMSGMRRAIASRLQTSKQQ
HCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHCC
SPHFRLSVDLDLERLLALRQDINREVPGVKISVNDLLVKACALALVAVPDVNIQFDEAAQ
CCCEEEEECCCHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCEEHHHHHH
SIRRFTDADISVAVALPAGLITPIVRSANRKSISDISNEIHSLVTRAKAGTLKPEEFQGG
HHHHHCCCCCEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCC
TFSLSNLGMLGVRQFDAIINPPQSAILAIGAGEVRAVVRDGQIVARQQMTVSLSCDHRVI
EEECCCCCHHHHHHHHHHCCCCCCEEEEECCCCEEEEEECCCEEEEEEEEEEECCCCEEE
DGAAGAAFLRELKRLIETPTLMFIQETSYAR
CCCHHHHHHHHHHHHHCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]