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The map label for this gene is mltD [H]

Identifier: 238893204

GI number: 238893204

Start: 1046401

End: 1047768

Strand: Reverse

Name: mltD [H]

Synonym: KP1_1070

Alternate gene names: 238893204

Gene position: 1047768-1046401 (Counterclockwise)

Preceding gene: 238893205

Following gene: 238893200

Centisome position: 19.96

GC content: 55.85

Gene sequence:

>1368_bases
ATGAAGGCACGAGCGATATTACTCGCCTCTGTCCTGCTGGTGGGGTGCCAGGCGTCTAAGCACGATGGCACCGTCGAACA
GCGAGCACAGAGCCTTTCTGCGGCTGGTCAAGGGGAAGCAGGCAAGTTCACAAGTCAAGCGCGCTGGTTAGATGATGGAA
CCTTCTACGCGCAAGACCAGGATCTGTGGACTTCCATAGGCGACGAGCTAAAGATGGGAATACCGGATAATCCCCGGATT
CGCGAACAGAAACAGAAGTACTTAAGAAATAAGAGCTATCTCCACGATGTAACTTTACGGGCAGAGCCGTATATGTACTG
GATAGCCGGGCAGGTTAAGAAACGTAACATGCCAATGGAACTGGTATTACTACCCATAGTGGAGAGCGCTTTTGACCCAC
ACGCGACGTCTGGCGCCAATGCCGCAGGCATTTGGCAGATCATTCCGAGCACAGGGCGCAATTATGGTTTAAAACAGACC
CGCAGTTACGATGCGCGTCGTGATGTCGTCGCGTCTACTACCGCGGCGCTGGACATGATGCAACGTCTGAACAAAATGTT
CGACGGCGACTGGTTGTTAACGGTCGCAGCCTATAACAGCGGCGAAGGCCGGGTCATGAAGGCAGTAAAAGCGAACCGTT
CGCGTGGCAAACCCACCGATTTCTGGTCGCTGTCTCTGCCGCATGAAACGAAAATCTACGTCCCGAAAATGCTGGCATTG
AGCGACATTCTCAAAAACAGCAAACGTTACGGCGTAAAGCTGCCTACGGCTGATGAAAGCCGTGCGCTGGCGCGCGTTCG
CCTCGACAGTCCGGTTGATATTTCTCAGCTCGCGGACATGGCCGGTATGCCGGTCAGCAAGCTGAAGACGTTCAATGCGG
GCGTAAAAGGGTCAACGCTGGGCGCGAGCGGGCCAAAGTACGTCATGGTGCCGCAGAAGCACGCCGCACAGCTGCGTGAA
TCGCTGGCCTCTGGCGACATTGCCGCCGTGCAGCCGACGCAGCTCGCGGACAATACGCCGCTGACCAGCCGTAGCTATAA
GGTGCGTTCCGGCGACACCATTTCCGGGATAGCTTCCCGTCTTGGTGTGACGACCCGCGATCTGCAGCAGTGGAATAACC
TGCGCGGCTCTGGATTAAAGGTTGGGCAGAATCTGGTTATTGGCGCAGGCAGCAGCGCCCAGCGTCTGGCGAACAACAGC
GATAGCATCACCTATCGCGTTCGTAAAGGCGATTCGCTGTCGAGCATCGCCAAACGTCACGGCGTTAATATTCGCGACGT
GATGCGCTGGAACAGCGATACCGACAACCTGCGCCCAGGCGATCAGCTAACGTTGTTTGTGAAAAACAGCGATCGACCAG
AGTCCTGA

Upstream 100 bases:

>100_bases
GGTTAAGGTCAAAGAAAGATGACTTCTGATATTTAATTCTTGTCATCGGCCAACTTCGCCGTTATCCTTGGTCGTCTTTT
AAGCAACTATTGACACACAC

Downstream 100 bases:

>100_bases
TACCTCTGGTTCGACAAAAAGGCACCGACTTCCCCGGTGCCTTTTTTATTGCCTAAATTAAACCGCTTTATGCGCTTCGA
ACAGAATGGTATCGCTGGTA

Product: membrane-bound lytic murein transglycosylase D

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MKARAILLASVLLVGCQASKHDGTVEQRAQSLSAAGQGEAGKFTSQARWLDDGTFYAQDQDLWTSIGDELKMGIPDNPRI
REQKQKYLRNKSYLHDVTLRAEPYMYWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQT
RSYDARRDVVASTTAALDMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAVKANRSRGKPTDFWSLSLPHETKIYVPKMLAL
SDILKNSKRYGVKLPTADESRALARVRLDSPVDISQLADMAGMPVSKLKTFNAGVKGSTLGASGPKYVMVPQKHAAQLRE
SLASGDIAAVQPTQLADNTPLTSRSYKVRSGDTISGIASRLGVTTRDLQQWNNLRGSGLKVGQNLVIGAGSSAQRLANNS
DSITYRVRKGDSLSSIAKRHGVNIRDVMRWNSDTDNLRPGDQLTLFVKNSDRPES

Sequences:

>Translated_455_residues
MKARAILLASVLLVGCQASKHDGTVEQRAQSLSAAGQGEAGKFTSQARWLDDGTFYAQDQDLWTSIGDELKMGIPDNPRI
REQKQKYLRNKSYLHDVTLRAEPYMYWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQT
RSYDARRDVVASTTAALDMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAVKANRSRGKPTDFWSLSLPHETKIYVPKMLAL
SDILKNSKRYGVKLPTADESRALARVRLDSPVDISQLADMAGMPVSKLKTFNAGVKGSTLGASGPKYVMVPQKHAAQLRE
SLASGDIAAVQPTQLADNTPLTSRSYKVRSGDTISGIASRLGVTTRDLQQWNNLRGSGLKVGQNLVIGAGSSAQRLANNS
DSITYRVRKGDSLSSIAKRHGVNIRDVMRWNSDTDNLRPGDQLTLFVKNSDRPES
>Mature_455_residues
MKARAILLASVLLVGCQASKHDGTVEQRAQSLSAAGQGEAGKFTSQARWLDDGTFYAQDQDLWTSIGDELKMGIPDNPRI
REQKQKYLRNKSYLHDVTLRAEPYMYWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQT
RSYDARRDVVASTTAALDMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAVKANRSRGKPTDFWSLSLPHETKIYVPKMLAL
SDILKNSKRYGVKLPTADESRALARVRLDSPVDISQLADMAGMPVSKLKTFNAGVKGSTLGASGPKYVMVPQKHAAQLRE
SLASGDIAAVQPTQLADNTPLTSRSYKVRSGDTISGIASRLGVTTRDLQQWNNLRGSGLKVGQNLVIGAGSSAQRLANNS
DSITYRVRKGDSLSSIAKRHGVNIRDVMRWNSDTDNLRPGDQLTLFVKNSDRPES

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=455, Percent_Identity=85.2747252747253, Blast_Score=796, Evalue=0.0,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 49954; Mature: 49954

Theoretical pI: Translated: 10.44; Mature: 10.44

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKARAILLASVLLVGCQASKHDGTVEQRAQSLSAAGQGEAGKFTSQARWLDDGTFYAQDQ
CCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEECCCCEEEECCH
DLWTSIGDELKMGIPDNPRIREQKQKYLRNKSYLHDVTLRAEPYMYWIAGQVKKRNMPME
HHHHHCCCCEECCCCCCCCHHHHHHHHHHCCCHHEEEEEECCCEEEEEECHHHCCCCCCE
LVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRSYDARRDVVASTTAALDMM
EEEHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHH
QRLNKMFDGDWLLTVAAYNSGEGRVMKAVKANRSRGKPTDFWSLSLPHETKIYVPKMLAL
HHHHHHCCCCEEEEEEEEECCCCCEEHHHHHCCCCCCCCCEEEEECCCCCEEEHHHHHHH
SDILKNSKRYGVKLPTADESRALARVRLDSPVDISQLADMAGMPVSKLKTFNAGVKGSTL
HHHHHCCCCCCEECCCCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCCC
GASGPKYVMVPQKHAAQLRESLASGDIAAVQPTQLADNTPLTSRSYKVRSGDTISGIASR
CCCCCEEEEECCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCEEECCCCHHHHHHHH
LGVTTRDLQQWNNLRGSGLKVGQNLVIGAGSSAQRLANNSDSITYRVRKGDSLSSIAKRH
HCCCHHHHHHHHCCCCCCEEECCEEEEECCCCHHHHCCCCCCEEEEEECCCCHHHHHHHH
GVNIRDVMRWNSDTDNLRPGDQLTLFVKNSDRPES
CCCHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCC
>Mature Secondary Structure
MKARAILLASVLLVGCQASKHDGTVEQRAQSLSAAGQGEAGKFTSQARWLDDGTFYAQDQ
CCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEECCCCEEEECCH
DLWTSIGDELKMGIPDNPRIREQKQKYLRNKSYLHDVTLRAEPYMYWIAGQVKKRNMPME
HHHHHCCCCEECCCCCCCCHHHHHHHHHHCCCHHEEEEEECCCEEEEEECHHHCCCCCCE
LVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRSYDARRDVVASTTAALDMM
EEEHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHH
QRLNKMFDGDWLLTVAAYNSGEGRVMKAVKANRSRGKPTDFWSLSLPHETKIYVPKMLAL
HHHHHHCCCCEEEEEEEEECCCCCEEHHHHHCCCCCCCCCEEEEECCCCCEEEHHHHHHH
SDILKNSKRYGVKLPTADESRALARVRLDSPVDISQLADMAGMPVSKLKTFNAGVKGSTL
HHHHHCCCCCCEECCCCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCCC
GASGPKYVMVPQKHAAQLRESLASGDIAAVQPTQLADNTPLTSRSYKVRSGDTISGIASR
CCCCCEEEEECCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCEEECCCCHHHHHHHH
LGVTTRDLQQWNNLRGSGLKVGQNLVIGAGSSAQRLANNSDSITYRVRKGDSLSSIAKRH
HCCCHHHHHHHHCCCCCCEEECCEEEEECCCCHHHHCCCCCCEEEEEECCCCHHHHHHHH
GVNIRDVMRWNSDTDNLRPGDQLTLFVKNSDRPES
CCCHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]