| Definition | Klebsiella pneumoniae NTUH-K2044 chromosome, complete genome. |
|---|---|
| Accession | NC_012731 |
| Length | 5,248,520 |
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The map label for this gene is dkgB [H]
Identifier: 238893199
GI number: 238893199
Start: 1041532
End: 1042335
Strand: Direct
Name: dkgB [H]
Synonym: KP1_1065
Alternate gene names: 238893199
Gene position: 1041532-1042335 (Clockwise)
Preceding gene: 238893198
Following gene: 238893201
Centisome position: 19.84
GC content: 55.6
Gene sequence:
>804_bases ATGGCAATCCCTGCATTTGGTCTTGGCACCTTCCGTCTGAAAGATGATGTTGTTATCGCATCCGTAAAAACCGCGCTGGA GCTGGGCTATCGTGCTATCGACACCGCGCAAATCTATGACAACGAAGCCGCGGTGGGTCAGGCTATCGCCGAAAGCGGCG TGCCGCGTGATGAGCTGTTCATCACTACCAAAATCTGGATCGAGAACCTGAGCAAAGACAAACTCATCGCCAGCCTGCAA GAAAGTCTGCAGAAGCTGCGCACGGACTATGTTGACCTGACGCTCATCCACTGGCCGTCACCGAACGATGCGGTTGCCGT TGAAGAGTTTATGGCAGCCCTGATGGAAGCGAAGAAGCTGGGTCTGACCCGTCAGATTGGTATCTCCAACTTCACTATTC CACTGATGGAGCGCGCGATTGCCGCCGTTGGCGCTGAAAACATTGCGACCAACCAGATTGAACTCTCGCCGTATCTGCAA AACAGTAAAGTCGTCGACTGGGCACGTGAGCACGGCATCCACATCACCTCTTACATGACGCTGGCTTATGGTAAGGCGCT GAAAGATGAAGTGATTGCCCGTATCGCGGCGAAACATAATGCGACTCCGGCGCAGGTTATCCTGGCATGGGCGATGGGCG AGGGGTACGCGGTGATCCCGTCCTCGACGAAGCGTGATAACCTGGCCAGCAACCTGAAGGCGCTGGATCTGCAGTTGGAT GATGAAGATCGACAGGCGATCGCTGCGCTGGATTGCAACGATCGGCTGGTGAGCCCGGAAGGTCTGGCGCCGCAGTGGGA TTAA
Upstream 100 bases:
>100_bases ATTCAGCAAAAATCCTCTGCATTAATCGCTCTTTTTCCCCTCCACACCATCAGCCATAATTTCATCCGTTAACCGAAATG AAGAAATAACGAGGAAAATC
Downstream 100 bases:
>100_bases TCACTCGCTACCGGTATGCCGTTCGCCTTGCAGACCGAGATGGTATTACGACAGCTCCTTCGGGAGCTGTTTTACGTGGT CGCTCAGAAAATCAATAAAT
Product: 2,5-diketo-D-gluconate reductase B
Products: NA
Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]
Number of amino acids: Translated: 267; Mature: 266
Protein sequence:
>267_residues MAIPAFGLGTFRLKDDVVIASVKTALELGYRAIDTAQIYDNEAAVGQAIAESGVPRDELFITTKIWIENLSKDKLIASLQ ESLQKLRTDYVDLTLIHWPSPNDAVAVEEFMAALMEAKKLGLTRQIGISNFTIPLMERAIAAVGAENIATNQIELSPYLQ NSKVVDWAREHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYAVIPSSTKRDNLASNLKALDLQLD DEDRQAIAALDCNDRLVSPEGLAPQWD
Sequences:
>Translated_267_residues MAIPAFGLGTFRLKDDVVIASVKTALELGYRAIDTAQIYDNEAAVGQAIAESGVPRDELFITTKIWIENLSKDKLIASLQ ESLQKLRTDYVDLTLIHWPSPNDAVAVEEFMAALMEAKKLGLTRQIGISNFTIPLMERAIAAVGAENIATNQIELSPYLQ NSKVVDWAREHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYAVIPSSTKRDNLASNLKALDLQLD DEDRQAIAALDCNDRLVSPEGLAPQWD >Mature_266_residues AIPAFGLGTFRLKDDVVIASVKTALELGYRAIDTAQIYDNEAAVGQAIAESGVPRDELFITTKIWIENLSKDKLIASLQE SLQKLRTDYVDLTLIHWPSPNDAVAVEEFMAALMEAKKLGLTRQIGISNFTIPLMERAIAAVGAENIATNQIELSPYLQN SKVVDWAREHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYAVIPSSTKRDNLASNLKALDLQLDD EDRQAIAALDCNDRLVSPEGLAPQWD
Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) [H]
COG id: COG0656
COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the aldo/keto reductase family [H]
Homologues:
Organism=Homo sapiens, GI310109922, Length=275, Percent_Identity=30.9090909090909, Blast_Score=142, Evalue=4e-34, Organism=Homo sapiens, GI223468663, Length=285, Percent_Identity=31.5789473684211, Blast_Score=139, Evalue=3e-33, Organism=Homo sapiens, GI24497577, Length=289, Percent_Identity=30.1038062283737, Blast_Score=138, Evalue=4e-33, Organism=Homo sapiens, GI5174391, Length=289, Percent_Identity=30.1038062283737, Blast_Score=138, Evalue=4e-33, Organism=Homo sapiens, GI4502049, Length=289, Percent_Identity=34.6020761245675, Blast_Score=138, Evalue=5e-33, Organism=Homo sapiens, GI45446745, Length=292, Percent_Identity=29.4520547945205, Blast_Score=136, Evalue=2e-32, Organism=Homo sapiens, GI4503285, Length=292, Percent_Identity=29.4520547945205, Blast_Score=136, Evalue=2e-32, Organism=Homo sapiens, GI24497585, Length=291, Percent_Identity=29.2096219931272, Blast_Score=136, Evalue=2e-32, Organism=Homo sapiens, GI5174695, Length=296, Percent_Identity=31.0810810810811, Blast_Score=136, Evalue=2e-32, Organism=Homo sapiens, GI310109920, Length=292, Percent_Identity=29.4520547945205, Blast_Score=136, Evalue=2e-32, Organism=Homo sapiens, GI93277124, Length=299, Percent_Identity=30.1003344481605, Blast_Score=134, Evalue=6e-32, Organism=Homo sapiens, GI5453543, Length=292, Percent_Identity=29.1095890410959, Blast_Score=134, Evalue=8e-32, Organism=Homo sapiens, GI24497583, Length=297, Percent_Identity=29.96632996633, Blast_Score=132, Evalue=2e-31, Organism=Homo sapiens, GI291291012, Length=270, Percent_Identity=31.1111111111111, Blast_Score=128, Evalue=6e-30, Organism=Homo sapiens, GI300116273, Length=272, Percent_Identity=30.5147058823529, Blast_Score=121, Evalue=8e-28, Organism=Homo sapiens, GI300116271, Length=278, Percent_Identity=29.4964028776978, Blast_Score=117, Evalue=1e-26, Organism=Homo sapiens, GI207028673, Length=104, Percent_Identity=37.5, Blast_Score=77, Evalue=1e-14, Organism=Homo sapiens, GI310109926, Length=104, Percent_Identity=37.5, Blast_Score=77, Evalue=1e-14, Organism=Homo sapiens, GI310109924, Length=155, Percent_Identity=27.741935483871, Blast_Score=75, Evalue=6e-14, Organism=Escherichia coli, GI1786400, Length=267, Percent_Identity=90.2621722846442, Blast_Score=496, Evalue=1e-142, Organism=Escherichia coli, GI87082198, Length=259, Percent_Identity=38.996138996139, Blast_Score=182, Evalue=2e-47, Organism=Escherichia coli, GI1788081, Length=267, Percent_Identity=29.9625468164794, Blast_Score=110, Evalue=8e-26, Organism=Escherichia coli, GI48994888, Length=238, Percent_Identity=28.5714285714286, Blast_Score=74, Evalue=1e-14, Organism=Escherichia coli, GI1787674, Length=263, Percent_Identity=27.7566539923954, Blast_Score=68, Evalue=7e-13, Organism=Caenorhabditis elegans, GI17550248, Length=271, Percent_Identity=37.2693726937269, Blast_Score=162, Evalue=2e-40, Organism=Caenorhabditis elegans, GI17537075, Length=291, Percent_Identity=37.1134020618557, Blast_Score=151, Evalue=3e-37, Organism=Caenorhabditis elegans, GI17537077, Length=289, Percent_Identity=36.6782006920415, Blast_Score=147, Evalue=4e-36, Organism=Caenorhabditis elegans, GI17561298, Length=274, Percent_Identity=31.7518248175182, Blast_Score=147, Evalue=7e-36, Organism=Caenorhabditis elegans, GI71998625, Length=266, Percent_Identity=31.9548872180451, Blast_Score=145, Evalue=2e-35, Organism=Caenorhabditis elegans, GI17552492, Length=269, Percent_Identity=31.9702602230483, Blast_Score=137, Evalue=5e-33, Organism=Caenorhabditis elegans, GI17561300, Length=276, Percent_Identity=30.4347826086957, Blast_Score=134, Evalue=6e-32, Organism=Caenorhabditis elegans, GI17566692, Length=285, Percent_Identity=30.8771929824561, Blast_Score=126, Evalue=1e-29, Organism=Caenorhabditis elegans, GI17538386, Length=277, Percent_Identity=31.7689530685921, Blast_Score=125, Evalue=2e-29, Organism=Caenorhabditis elegans, GI17564128, Length=291, Percent_Identity=27.4914089347079, Blast_Score=122, Evalue=2e-28, Organism=Caenorhabditis elegans, GI17537079, Length=294, Percent_Identity=28.2312925170068, Blast_Score=120, Evalue=6e-28, Organism=Caenorhabditis elegans, GI17562292, Length=268, Percent_Identity=28.3582089552239, Blast_Score=112, Evalue=1e-25, Organism=Saccharomyces cerevisiae, GI6324694, Length=286, Percent_Identity=35.3146853146853, Blast_Score=169, Evalue=6e-43, Organism=Saccharomyces cerevisiae, GI6320576, Length=268, Percent_Identity=36.1940298507463, Blast_Score=160, Evalue=1e-40, Organism=Saccharomyces cerevisiae, GI6321896, Length=306, Percent_Identity=29.4117647058824, Blast_Score=126, Evalue=3e-30, Organism=Saccharomyces cerevisiae, GI6322556, Length=259, Percent_Identity=26.6409266409266, Blast_Score=119, Evalue=6e-28, Organism=Saccharomyces cerevisiae, GI6319625, Length=276, Percent_Identity=31.5217391304348, Blast_Score=114, Evalue=1e-26, Organism=Saccharomyces cerevisiae, GI6320079, Length=267, Percent_Identity=30.3370786516854, Blast_Score=99, Evalue=5e-22, Organism=Drosophila melanogaster, GI24662789, Length=293, Percent_Identity=33.1058020477816, Blast_Score=145, Evalue=3e-35, Organism=Drosophila melanogaster, GI24657054, Length=284, Percent_Identity=31.6901408450704, Blast_Score=136, Evalue=1e-32, Organism=Drosophila melanogaster, GI24644950, Length=298, Percent_Identity=32.5503355704698, Blast_Score=136, Evalue=2e-32, Organism=Drosophila melanogaster, GI24662785, Length=289, Percent_Identity=35.2941176470588, Blast_Score=133, Evalue=1e-31, Organism=Drosophila melanogaster, GI24663317, Length=285, Percent_Identity=29.8245614035088, Blast_Score=132, Evalue=2e-31, Organism=Drosophila melanogaster, GI20129731, Length=286, Percent_Identity=31.4685314685315, Blast_Score=132, Evalue=3e-31, Organism=Drosophila melanogaster, GI221378297, Length=317, Percent_Identity=30.2839116719243, Blast_Score=129, Evalue=2e-30, Organism=Drosophila melanogaster, GI24662781, Length=289, Percent_Identity=34.6020761245675, Blast_Score=129, Evalue=3e-30, Organism=Drosophila melanogaster, GI45553081, Length=283, Percent_Identity=29.3286219081272, Blast_Score=127, Evalue=7e-30, Organism=Drosophila melanogaster, GI21356425, Length=286, Percent_Identity=31.1188811188811, Blast_Score=127, Evalue=8e-30, Organism=Drosophila melanogaster, GI281366140, Length=258, Percent_Identity=27.5193798449612, Blast_Score=106, Evalue=2e-23,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001395 - InterPro: IPR018170 - InterPro: IPR020471 - InterPro: IPR023210 [H]
Pfam domain/function: PF00248 Aldo_ket_red [H]
EC number: =1.1.1.274 [H]
Molecular weight: Translated: 29245; Mature: 29114
Theoretical pI: Translated: 4.62; Mature: 4.62
Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.4 %Cys (Translated Protein) 2.2 %Met (Translated Protein) 2.6 %Cys+Met (Translated Protein) 0.4 %Cys (Mature Protein) 1.9 %Met (Mature Protein) 2.3 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MAIPAFGLGTFRLKDDVVIASVKTALELGYRAIDTAQIYDNEAAVGQAIAESGVPRDELF CCCCCCCCEEEEEECCEEEHHHHHHHHHCHHHCCHHHHCCCHHHHHHHHHHCCCCHHHEE ITTKIWIENLSKDKLIASLQESLQKLRTDYVDLTLIHWPSPNDAVAVEEFMAALMEAKKL EEEEHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHC GLTRQIGISNFTIPLMERAIAAVGAENIATNQIELSPYLQNSKVVDWAREHGIHITSYMT CCEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHCCEEEEEHHH LAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYAVIPSSTKRDNLASNLKALDLQLD HHHHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCCEEECCCCCCCHHHHHCCEEEEEEEC DEDRQAIAALDCNDRLVSPEGLAPQWD CCCCCEEEEECCCCCEECCCCCCCCCC >Mature Secondary Structure AIPAFGLGTFRLKDDVVIASVKTALELGYRAIDTAQIYDNEAAVGQAIAESGVPRDELF CCCCCCCEEEEEECCEEEHHHHHHHHHCHHHCCHHHHCCCHHHHHHHHHHCCCCHHHEE ITTKIWIENLSKDKLIASLQESLQKLRTDYVDLTLIHWPSPNDAVAVEEFMAALMEAKKL EEEEHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHC GLTRQIGISNFTIPLMERAIAAVGAENIATNQIELSPYLQNSKVVDWAREHGIHITSYMT CCEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHCCEEEEEHHH LAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYAVIPSSTKRDNLASNLKALDLQLD HHHHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCCEEECCCCCCCHHHHHCCEEEEEEEC DEDRQAIAALDCNDRLVSPEGLAPQWD CCCCCEEEEECCCCCEECCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 11206551; 11258796; 11108008 [H]