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The map label for this gene is pulD [H]

Identifier: 238893139

GI number: 238893139

Start: 968510

End: 970483

Strand: Reverse

Name: pulD [H]

Synonym: KP1_0990

Alternate gene names: 238893139

Gene position: 970483-968510 (Counterclockwise)

Preceding gene: 238893140

Following gene: 238893138

Centisome position: 18.49

GC content: 58.41

Gene sequence:

>1974_bases
ATGAAACGCCTGCGAAAAATGCTGCCTGCGCTGCTGATATTAACCCCGCTGCTATTTTCTCCGGCCGCCGCGGAGGAATT
TTCCGCCAGTTTTAAAGGCACCGATATCCAGGAATTTATCAATACCGTCAGCAAAAACCTGAATAAAACGGTGATTATCG
ATCCCAGCGTACGCGGTACTATCACCGTGCGCAGTTACGATATGCTCAATGAAGAACAATATTATCAATTCTTCCTCAGC
GTGCTGGATGTATATGGCTTCGCCGTGATCAATATGAATAACGGCGTGCTAAAAGTGGTCCGCGCCAAAGACGCCAAGAC
CTCGGCCGTGCCGGTCGCCAGCGCGGCCGCGCCGGGCGAAGGCGATGAAGTGGTCACCCGGGTGGTGCCCCTGACCAACG
TCGCCGCCCGCGATCTCGCCCCGCTGCTCCGCCAGCTGAACGATAACGCTGGCGCCGGCAGCGTGGTCCATTATGAGCCC
TCCAACGTACTGCTGATGACCGGCCGCGCGGCGGTTATCAAACGCCTGCTGACCATTGTCGAGCGCGTCGACAACGCCGG
GGATCGCAGCGTGGTGACCGTGCCCCTTTCCTGGGCCTCCGCCGCGGAAGTAGTCAAACTGGTTACCGAGCTTAATAAAG
ATACCAGCAAGTCGGCGCTGCCGGGCTCGATGGTGGCGAACGTGGTGGCCGATGAGCGCACCAACGCGGTACTGGTGAGC
GGCGAACCAAACTCGCGCCAGCGCATTATCGCCATGATCAAACAGCTCGACCGCCAGCAGGCGGTACAGGGTAACACCAA
AGTGATCTACCTGAAGTACGCCAAAGCCGCCGATCTGGTGGAAGTTCTGACCGGGATCAGCAGCTCGCTGCAGAGCGACA
AGCAAAGCGCCAGGCCAGTGGCCGCGATCGACAAAAATATTATTATCAAAGCCCACGGCCAGACCAACGCCCTGATCGTC
ACCGCGGCGCCAGACGTGATGAACGATCTTGAGCGGGTGATCGCCCAGCTCGATATCCGCCGCCCGCAGGTGCTGGTGGA
GGCGATTATTGCCGAAGTGCAGGATGCCGATGGCCTTAATCTCGGTATCCAGTGGGCAAACAAAAACGCCGGCATGACGC
AGTTCACCAATAGCGGTTTGCCGATATCGACGGCTATCGCCGGCGCCAACCAGTACAACAAAGACGGTACCATCAGCAGC
TCGCTGGCCAGCGCCCTCGGCTCGTTTAATGGTATCGCCGCCGGCTTCTATCAGGGGAACTGGGCGATGCTGCTCACCGC
CCTCTCCAGCAGTACCAAGAACGATATTCTGGCCACCCCCAGCATCGTCACCCTCGATAACATGCAAGCCACTTTTAACG
TCGGCCAGGAAGTTCCGGTATTAACCGGCTCGCAAACCACCTCAGGCGACAACATCTTCAATACCGTCGAGCGCAAAACC
GTCGGCATTAAACTGAAGGTGAAACCGCAGATTAACGAAGGCGATGCGGTGCTGCTGGAGATTGAGCAGGAGGTATCGAG
CGTTGCCGACTCGGCCTCCAGCACCAGCAGCGATCTCGGGGCGACCTTCAATACCCGGACGGTGAATAACGCCGTGCTGG
TCGGCAGCGGCGAAACGGTGGTGGTTGGCGGGCTGCTGGATAAAACCGTCACCGATACCGCTGACAAAGTGCCGCTGCTC
GGGGATATCCCGGTGATCGGCGCCCTGTTCCGCTCCGACAGCAAGAAAGTGTCGAAACGCAACCTGATGCTGTTTATCCG
CCCAACCATCATTCGCGATCGCGATGAGTACCGCCAGGCTTCTTCCGGCCAGTACACCGCCTTCAACAATGCGCAAACTA
AACAGCGGGGTAAAGAAAGCAGCGAAGCGTCGCTCAGCAACGACCTGCTGCATATCTATCCGCAGCAGGAGACCCAGGCT
TTCCGCCAGGTCAGCGCCGCCATTGACGCCTTCAATCTCGGAGGCCGCCCATGA

Upstream 100 bases:

>100_bases
GCAAAATCTGGCCGACATGACCGAAATAACGCTAACCGTTGAGCGGGAAGGCCAACGACACGATATTGCTTTCGCGCTGG
GGGATGAATAATTGCCAACC

Downstream 100 bases:

>100_bases
CGCCAGCCGCCGAACGCCGTCCGCTGTTGCCCTTCGCCTGGGCCCGCGCGCACCATCTTGTGCTGCTCAGCGACGGCGAA
TGCTGCGAAGCGCTGTGCCG

Product: pullulanase-specific type II secretion system secretin

Products: NA

Alternate protein names: Pullulanase secretion envelope pulD [H]

Number of amino acids: Translated: 657; Mature: 657

Protein sequence:

>657_residues
MKRLRKMLPALLILTPLLFSPAAAEEFSASFKGTDIQEFINTVSKNLNKTVIIDPSVRGTITVRSYDMLNEEQYYQFFLS
VLDVYGFAVINMNNGVLKVVRAKDAKTSAVPVASAAAPGEGDEVVTRVVPLTNVAARDLAPLLRQLNDNAGAGSVVHYEP
SNVLLMTGRAAVIKRLLTIVERVDNAGDRSVVTVPLSWASAAEVVKLVTELNKDTSKSALPGSMVANVVADERTNAVLVS
GEPNSRQRIIAMIKQLDRQQAVQGNTKVIYLKYAKAADLVEVLTGISSSLQSDKQSARPVAAIDKNIIIKAHGQTNALIV
TAAPDVMNDLERVIAQLDIRRPQVLVEAIIAEVQDADGLNLGIQWANKNAGMTQFTNSGLPISTAIAGANQYNKDGTISS
SLASALGSFNGIAAGFYQGNWAMLLTALSSSTKNDILATPSIVTLDNMQATFNVGQEVPVLTGSQTTSGDNIFNTVERKT
VGIKLKVKPQINEGDAVLLEIEQEVSSVADSASSTSSDLGATFNTRTVNNAVLVGSGETVVVGGLLDKTVTDTADKVPLL
GDIPVIGALFRSDSKKVSKRNLMLFIRPTIIRDRDEYRQASSGQYTAFNNAQTKQRGKESSEASLSNDLLHIYPQQETQA
FRQVSAAIDAFNLGGRP

Sequences:

>Translated_657_residues
MKRLRKMLPALLILTPLLFSPAAAEEFSASFKGTDIQEFINTVSKNLNKTVIIDPSVRGTITVRSYDMLNEEQYYQFFLS
VLDVYGFAVINMNNGVLKVVRAKDAKTSAVPVASAAAPGEGDEVVTRVVPLTNVAARDLAPLLRQLNDNAGAGSVVHYEP
SNVLLMTGRAAVIKRLLTIVERVDNAGDRSVVTVPLSWASAAEVVKLVTELNKDTSKSALPGSMVANVVADERTNAVLVS
GEPNSRQRIIAMIKQLDRQQAVQGNTKVIYLKYAKAADLVEVLTGISSSLQSDKQSARPVAAIDKNIIIKAHGQTNALIV
TAAPDVMNDLERVIAQLDIRRPQVLVEAIIAEVQDADGLNLGIQWANKNAGMTQFTNSGLPISTAIAGANQYNKDGTISS
SLASALGSFNGIAAGFYQGNWAMLLTALSSSTKNDILATPSIVTLDNMQATFNVGQEVPVLTGSQTTSGDNIFNTVERKT
VGIKLKVKPQINEGDAVLLEIEQEVSSVADSASSTSSDLGATFNTRTVNNAVLVGSGETVVVGGLLDKTVTDTADKVPLL
GDIPVIGALFRSDSKKVSKRNLMLFIRPTIIRDRDEYRQASSGQYTAFNNAQTKQRGKESSEASLSNDLLHIYPQQETQA
FRQVSAAIDAFNLGGRP
>Mature_657_residues
MKRLRKMLPALLILTPLLFSPAAAEEFSASFKGTDIQEFINTVSKNLNKTVIIDPSVRGTITVRSYDMLNEEQYYQFFLS
VLDVYGFAVINMNNGVLKVVRAKDAKTSAVPVASAAAPGEGDEVVTRVVPLTNVAARDLAPLLRQLNDNAGAGSVVHYEP
SNVLLMTGRAAVIKRLLTIVERVDNAGDRSVVTVPLSWASAAEVVKLVTELNKDTSKSALPGSMVANVVADERTNAVLVS
GEPNSRQRIIAMIKQLDRQQAVQGNTKVIYLKYAKAADLVEVLTGISSSLQSDKQSARPVAAIDKNIIIKAHGQTNALIV
TAAPDVMNDLERVIAQLDIRRPQVLVEAIIAEVQDADGLNLGIQWANKNAGMTQFTNSGLPISTAIAGANQYNKDGTISS
SLASALGSFNGIAAGFYQGNWAMLLTALSSSTKNDILATPSIVTLDNMQATFNVGQEVPVLTGSQTTSGDNIFNTVERKT
VGIKLKVKPQINEGDAVLLEIEQEVSSVADSASSTSSDLGATFNTRTVNNAVLVGSGETVVVGGLLDKTVTDTADKVPLL
GDIPVIGALFRSDSKKVSKRNLMLFIRPTIIRDRDEYRQASSGQYTAFNNAQTKQRGKESSEASLSNDLLHIYPQQETQA
FRQVSAAIDAFNLGGRP

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pullulanase [H]

COG id: COG1450

COG function: function code NU; Type II secretory pathway, component PulD

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI87082242, Length=637, Percent_Identity=55.4160125588697, Blast_Score=676, Evalue=0.0,
Organism=Escherichia coli, GI1789793, Length=327, Percent_Identity=25.9938837920489, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR004846
- InterPro:   IPR013356
- InterPro:   IPR004845 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N [H]

EC number: NA

Molecular weight: Translated: 70333; Mature: 70333

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00875 T2SP_D

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLRKMLPALLILTPLLFSPAAAEEFSASFKGTDIQEFINTVSKNLNKTVIIDPSVRGT
CHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCEE
ITVRSYDMLNEEQYYQFFLSVLDVYGFAVINMNNGVLKVVRAKDAKTSAVPVASAAAPGE
EEEEEEHHCCHHHHHHHHHHHHHHHCEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCC
GDEVVTRVVPLTNVAARDLAPLLRQLNDNAGAGSVVHYEPSNVLLMTGRAAVIKRLLTIV
CHHHHHHECCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEECCHHHHHHHHHHH
ERVDNAGDRSVVTVPLSWASAAEVVKLVTELNKDTSKSALPGSMVANVVADERTNAVLVS
HHHCCCCCCEEEEEEECHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCEEEEE
GEPNSRQRIIAMIKQLDRQQAVQGNTKVIYLKYAKAADLVEVLTGISSSLQSDKQSARPV
CCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCE
AAIDKNIIIKAHGQTNALIVTAAPDVMNDLERVIAQLDIRRPQVLVEAIIAEVQDADGLN
EEECCCEEEEECCCCCEEEEEECHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCE
LGIQWANKNAGMTQFTNSGLPISTAIAGANQYNKDGTISSSLASALGSFNGIAAGFYQGN
EEEEEECCCCCCCEECCCCCCHHHEECCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCC
WAMLLTALSSSTKNDILATPSIVTLDNMQATFNVGQEVPVLTGSQTTSGDNIFNTVERKT
HHHHHHHHHCCCCCCEEECCCEEEECCCEEEECCCCCCCEEECCCCCCCCHHHHHHHHEE
VGIKLKVKPQINEGDAVLLEIEQEVSSVADSASSTSSDLGATFNTRTVNNAVLVGSGETV
EEEEEEECCCCCCCCEEEEEEHHHHHHHHHCCCCCCCCCCCEEECEECCCEEEEECCCEE
VVGGLLDKTVTDTADKVPLLGDIPVIGALFRSDSKKVSKRNLMLFIRPTIIRDRDEYRQA
EECCCHHHHHHCCCCCCCCCCCCHHHHHHHHCCCHHHCCCCEEEEEECCEECCCHHHHHC
SSGQYTAFNNAQTKQRGKESSEASLSNDLLHIYPQQETQAFRQVSAAIDAFNLGGRP
CCCCEEEECCCHHHHHCCCCCHHHHCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKRLRKMLPALLILTPLLFSPAAAEEFSASFKGTDIQEFINTVSKNLNKTVIIDPSVRGT
CHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCEE
ITVRSYDMLNEEQYYQFFLSVLDVYGFAVINMNNGVLKVVRAKDAKTSAVPVASAAAPGE
EEEEEEHHCCHHHHHHHHHHHHHHHCEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCC
GDEVVTRVVPLTNVAARDLAPLLRQLNDNAGAGSVVHYEPSNVLLMTGRAAVIKRLLTIV
CHHHHHHECCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEECCHHHHHHHHHHH
ERVDNAGDRSVVTVPLSWASAAEVVKLVTELNKDTSKSALPGSMVANVVADERTNAVLVS
HHHCCCCCCEEEEEEECHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCEEEEE
GEPNSRQRIIAMIKQLDRQQAVQGNTKVIYLKYAKAADLVEVLTGISSSLQSDKQSARPV
CCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCE
AAIDKNIIIKAHGQTNALIVTAAPDVMNDLERVIAQLDIRRPQVLVEAIIAEVQDADGLN
EEECCCEEEEECCCCCEEEEEECHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCE
LGIQWANKNAGMTQFTNSGLPISTAIAGANQYNKDGTISSSLASALGSFNGIAAGFYQGN
EEEEEECCCCCCCEECCCCCCHHHEECCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCC
WAMLLTALSSSTKNDILATPSIVTLDNMQATFNVGQEVPVLTGSQTTSGDNIFNTVERKT
HHHHHHHHHCCCCCCEEECCCEEEECCCEEEECCCCCCCEEECCCCCCCCHHHHHHHHEE
VGIKLKVKPQINEGDAVLLEIEQEVSSVADSASSTSSDLGATFNTRTVNNAVLVGSGETV
EEEEEEECCCCCCCCEEEEEEHHHHHHHHHCCCCCCCCCCCEEECEECCCEEEEECCCEE
VVGGLLDKTVTDTADKVPLLGDIPVIGALFRSDSKKVSKRNLMLFIRPTIIRDRDEYRQA
EECCCHHHHHHCCCCCCCCCCCCHHHHHHHHCCCHHHCCCCEEEEEECCEECCCHHHHHC
SSGQYTAFNNAQTKQRGKESSEASLSNDLLHIYPQQETQAFRQVSAAIDAFNLGGRP
CCCCEEEECCCHHHHHCCCCCHHHHCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2677007 [H]