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The map label for this gene is pulE [H]

Identifier: 238893138

GI number: 238893138

Start: 967020

End: 968513

Strand: Reverse

Name: pulE [H]

Synonym: KP1_0989

Alternate gene names: 238893138

Gene position: 968513-967020 (Counterclockwise)

Preceding gene: 238893139

Following gene: 238893137

Centisome position: 18.45

GC content: 65.6

Gene sequence:

>1494_bases
ATGACGCCAGCCGCCGAACGCCGTCCGCTGTTGCCCTTCGCCTGGGCCCGCGCGCACCATCTTGTGCTGCTCAGCGACGG
CGAATGCTGCGAAGCGCTGTGCCGCCCCGATACCGCCGCCCGGGCGCTGCTGGAAGCGCGCCGCCTGGCGGACGGGCCGA
TGCGCGTCAGCCGTCTGGCGCCGGAGGCATTCGAGAAAGTGCTGGTGCTCAGCTATCAGCGTGATTCGGCGGAGGCGCAC
CGCATGATGGCCGACATCGGCAATGAGCTCGACCTCTATACCCTCGCCGAAGAGCTACCGGACACCGACGATCTGCTCGA
CAGCGAGGACGACGCGCCGATCATCCGCCTGATCAACGCCATGCTCACCGAGGCCATCAAAGAGAAAGCCTCGGATATTC
ATATCGAAACCTACGAGCGCCATCTGCAGATCCGCTTTCGCGTCGACGGCGTGCTGCGGGAAATTCTGCGCCCGCAGCGG
CGGCTGGCCGCCCTGTTAATTTCCCGCATCAAGGTGATGGCCAGCCTGGACATTGCCGAAAAGCGGGTCCCTCAGGATGG
CCGCATGGCGCTGCGCATCGGCGGGCGAGCAATCGACGTGCGCGTCTCCACCCTGCCCTCCAGCCACGGCGAACGCGTCG
TCCTGCGTCTGCTGGACAAAAACAGCGTCAACCTGGATCTGCTGACGCTGGGCATGCCGCCCGCCCTGCTCGATCGGGTC
GATGCGCTGATCGCCCGCCCGCACGGCATCATCCTTGTCACCGGTCCGACCGGCTCCGGGAAAAGCACGACGCTCTACGC
CGCCCTCAGCCGCCTCGACGCCCGGGAGCGCAACATCATGACCATTGAAGATCCGGTGGAGTATGAGCTGGAGGGCATAG
GCCAGACTCAGGTCAACGCCAAAGTCGAGATGACCTTCGCCCGCGGCCTGCGCGCCATCCTGCGCCAGGATCCGGACGTA
GTGCTGGTGGGCGAAATCCGCGATGGCGAAACCGCCCAGATCGCCGTTCAGGCCTCGCTGACCGGGCACCTGGTGCTGTC
GACCCTGCACACCAACAGCGCGCTGGGCGCCATTTCCCGTCTACAGGATATGGGCGTGGAGCCTTTTTTACTCTCCACCT
CGCTGCTGGCGGTGATGTCGCAGCGGCTGGTGCGCCAACTGTGCCCACACTGCCGTCAGCCCTGGCAGGCGGATGCCAAC
ACCGCCCGGCAGATGGCGGTCCCGGTCGGCGCGCGGCTGTGGCAGCCCAAAGGCTGTCCGGAGTGTAATTTCATCGGCTA
TCGCGGACGCACCGGTATTCATGAACTGCTGCTGGTTGACGACCGCGTCCGCGCCGCCATCCACCGCGGCGAGAATGAGA
TCACTCTGATCCAGCAGCTGGGGCCAGCCTGGCAAACCCTGCGCCATGCCGGACGCGACAAAGCGCTGGCGGGGATCACC
AGCTGGGAGGAGGTGATGCGGGTCACCGAACAGCAAACGACGGAAAGCGTCTAA

Upstream 100 bases:

>100_bases
CGCTCAGCAACGACCTGCTGCATATCTATCCGCAGCAGGAGACCCAGGCTTTCCGCCAGGTCAGCGCCGCCATTGACGCC
TTCAATCTCGGAGGCCGCCC

Downstream 100 bases:

>100_bases
GATGGCCCTGTTTCGCTATCAGGCGCTGGACGCGCAGGGCAAAACCCGCCGCGGCCTTCAGCAGGCCGACTCCGCCCGCC
ACGCCCGCCAGCTGCTGCGG

Product: pullulanase-specific type II secretion system ATPase

Products: NA

Alternate protein names: Pullulanase secretion protein pulE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MTPAAERRPLLPFAWARAHHLVLLSDGECCEALCRPDTAARALLEARRLADGPMRVSRLAPEAFEKVLVLSYQRDSAEAH
RMMADIGNELDLYTLAEELPDTDDLLDSEDDAPIIRLINAMLTEAIKEKASDIHIETYERHLQIRFRVDGVLREILRPQR
RLAALLISRIKVMASLDIAEKRVPQDGRMALRIGGRAIDVRVSTLPSSHGERVVLRLLDKNSVNLDLLTLGMPPALLDRV
DALIARPHGIILVTGPTGSGKSTTLYAALSRLDARERNIMTIEDPVEYELEGIGQTQVNAKVEMTFARGLRAILRQDPDV
VLVGEIRDGETAQIAVQASLTGHLVLSTLHTNSALGAISRLQDMGVEPFLLSTSLLAVMSQRLVRQLCPHCRQPWQADAN
TARQMAVPVGARLWQPKGCPECNFIGYRGRTGIHELLLVDDRVRAAIHRGENEITLIQQLGPAWQTLRHAGRDKALAGIT
SWEEVMRVTEQQTTESV

Sequences:

>Translated_497_residues
MTPAAERRPLLPFAWARAHHLVLLSDGECCEALCRPDTAARALLEARRLADGPMRVSRLAPEAFEKVLVLSYQRDSAEAH
RMMADIGNELDLYTLAEELPDTDDLLDSEDDAPIIRLINAMLTEAIKEKASDIHIETYERHLQIRFRVDGVLREILRPQR
RLAALLISRIKVMASLDIAEKRVPQDGRMALRIGGRAIDVRVSTLPSSHGERVVLRLLDKNSVNLDLLTLGMPPALLDRV
DALIARPHGIILVTGPTGSGKSTTLYAALSRLDARERNIMTIEDPVEYELEGIGQTQVNAKVEMTFARGLRAILRQDPDV
VLVGEIRDGETAQIAVQASLTGHLVLSTLHTNSALGAISRLQDMGVEPFLLSTSLLAVMSQRLVRQLCPHCRQPWQADAN
TARQMAVPVGARLWQPKGCPECNFIGYRGRTGIHELLLVDDRVRAAIHRGENEITLIQQLGPAWQTLRHAGRDKALAGIT
SWEEVMRVTEQQTTESV
>Mature_496_residues
TPAAERRPLLPFAWARAHHLVLLSDGECCEALCRPDTAARALLEARRLADGPMRVSRLAPEAFEKVLVLSYQRDSAEAHR
MMADIGNELDLYTLAEELPDTDDLLDSEDDAPIIRLINAMLTEAIKEKASDIHIETYERHLQIRFRVDGVLREILRPQRR
LAALLISRIKVMASLDIAEKRVPQDGRMALRIGGRAIDVRVSTLPSSHGERVVLRLLDKNSVNLDLLTLGMPPALLDRVD
ALIARPHGIILVTGPTGSGKSTTLYAALSRLDARERNIMTIEDPVEYELEGIGQTQVNAKVEMTFARGLRAILRQDPDVV
LVGEIRDGETAQIAVQASLTGHLVLSTLHTNSALGAISRLQDMGVEPFLLSTSLLAVMSQRLVRQLCPHCRQPWQADANT
ARQMAVPVGARLWQPKGCPECNFIGYRGRTGIHELLLVDDRVRAAIHRGENEITLIQQLGPAWQTLRHAGRDKALAGITS
WEEVMRVTEQQTTESV

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pullulanase [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=483, Percent_Identity=58.592132505176, Blast_Score=536, Evalue=1e-153,
Organism=Escherichia coli, GI1786296, Length=383, Percent_Identity=44.3864229765013, Blast_Score=307, Evalue=1e-84,
Organism=Escherichia coli, GI87082188, Length=281, Percent_Identity=38.0782918149466, Blast_Score=127, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 55141; Mature: 55010

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPAAERRPLLPFAWARAHHLVLLSDGECCEALCRPDTAARALLEARRLADGPMRVSRLA
CCCCCCCCCCCCCHHCCCEEEEEECCCHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHCC
PEAFEKVLVLSYQRDSAEAHRMMADIGNELDLYTLAEELPDTDDLLDSEDDAPIIRLINA
HHHHHHHHHEEECCCHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHCCCCCCCHHHHHHHH
MLTEAIKEKASDIHIETYERHLQIRFRVDGVLREILRPQRRLAALLISRIKVMASLDIAE
HHHHHHHHHHCCCCCEEEECEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRVPQDGRMALRIGGRAIDVRVSTLPSSHGERVVLRLLDKNSVNLDLLTLGMPPALLDRV
HHCCCCCCEEEEECCEEEEEEEEECCCCCCCEEEEEEECCCCCCEEEEEECCCHHHHHHH
DALIARPHGIILVTGPTGSGKSTTLYAALSRLDARERNIMTIEDPVEYELEGIGQTQVNA
HHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCHHHCCCCCEECCC
KVEMTFARGLRAILRQDPDVVLVGEIRDGETAQIAVQASLTGHLVLSTLHTNSALGAISR
EEEHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEEECCCEEEEEEHHCCHHHHHHHH
LQDMGVEPFLLSTSLLAVMSQRLVRQLCPHCRQPWQADANTARQMAVPVGARLWQPKGCP
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCC
ECNFIGYRGRTGIHELLLVDDRVRAAIHRGENEITLIQQLGPAWQTLRHAGRDKALAGIT
CCCEECCCCCCCHHHHEEECHHHHHHHHCCCCCEEHHHHCCHHHHHHHHCCCCHHHHCCC
SWEEVMRVTEQQTTESV
CHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TPAAERRPLLPFAWARAHHLVLLSDGECCEALCRPDTAARALLEARRLADGPMRVSRLA
CCCCCCCCCCCCHHCCCEEEEEECCCHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHCC
PEAFEKVLVLSYQRDSAEAHRMMADIGNELDLYTLAEELPDTDDLLDSEDDAPIIRLINA
HHHHHHHHHEEECCCHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHCCCCCCCHHHHHHHH
MLTEAIKEKASDIHIETYERHLQIRFRVDGVLREILRPQRRLAALLISRIKVMASLDIAE
HHHHHHHHHHCCCCCEEEECEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRVPQDGRMALRIGGRAIDVRVSTLPSSHGERVVLRLLDKNSVNLDLLTLGMPPALLDRV
HHCCCCCCEEEEECCEEEEEEEEECCCCCCCEEEEEEECCCCCCEEEEEECCCHHHHHHH
DALIARPHGIILVTGPTGSGKSTTLYAALSRLDARERNIMTIEDPVEYELEGIGQTQVNA
HHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCHHHCCCCCEECCC
KVEMTFARGLRAILRQDPDVVLVGEIRDGETAQIAVQASLTGHLVLSTLHTNSALGAISR
EEEHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEEECCCEEEEEEHHCCHHHHHHHH
LQDMGVEPFLLSTSLLAVMSQRLVRQLCPHCRQPWQADANTARQMAVPVGARLWQPKGCP
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCC
ECNFIGYRGRTGIHELLLVDDRVRAAIHRGENEITLIQQLGPAWQTLRHAGRDKALAGIT
CCCEECCCCCCCHHHHEEECHHHHHHHHCCCCCEEHHHHCCHHHHHHHHCCCCHHHHCCC
SWEEVMRVTEQQTTESV
CHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1738317; 2677007 [H]