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The map label for this gene is yfkN [H]

Identifier: 238892344

GI number: 238892344

Start: 95769

End: 97325

Strand: Direct

Name: yfkN [H]

Synonym: KP1_0099

Alternate gene names: 238892344

Gene position: 95769-97325 (Clockwise)

Preceding gene: 238892342

Following gene: 238892346

Centisome position: 1.82

GC content: 51.57

Gene sequence:

>1557_bases
ATGAAACTAAAAGCAATGAGCGCAGCACTTCTGTTGACTTTGCCTTTCTGGGCCTGCGCCAAAGATGTCACCATAATTTA
TACCAACGATCTTCATGCCCATGTCGAACCTTATAAAGTGCCATGGATAGCGGATGGTAAACGAGATATTGGCGGTTGGG
CGAATATCACGACGATGGTGAAACAGGAAAAAGCGAAAAATAACGCGACCTGGTTTTTTGATGCCGGAGATTATTTTACC
GGGCCTTACATCAGTAGCCTGACGAAGGGCAAAGCGATAATCGATATTATGAATACGATGTCCTACGATGCGGTGACTAT
CGGTAATCATGAGTTCGATCACGGCTGGGATAATACGTTGCTTCAGCTTAGCCAGGCGACATTCCCGATTGTTCAGGGCA
ATATTTTTTATCAGAACAGCGATAAATCGTTCTGGGATAAGCCCTATACCATTATCGAAAAAGATGGCGTGAAAATTGGC
GTGATTGGCCTGCACGGCGTGTTTGCTTTTAATGACACCGTTTCTGCCGCGACGCGAGTGGGTATCGAAGCGCGCGATGA
GGTGAAATGGCTACAGCATTATATCGATGAACTTAACGGTAAAGTGGATCTGACCGTGGCCCTGATCCATGAAGGCGTCC
CGGCGCGTCAGTCGAGCCAGGGGGGAACCGATGTGCGTCGTGCGCTGGATAAAGATATTCAGACTGCGGGTCAGGTGAAA
GGTCTGGACGTTTTAATTACCGGCCATGCTCACGTTGGCACACCGGAGCCCATTAAAGTGGGTAATACGTTGATCCTTTC
TACCGATAGCGGCGGCATCGATGTGGGTAAACTGGTGCTGGATTATCAGGAGAAACCGCACCAATTCACGGTGAAGCACT
TTGAGCTGAAGACGCTTTATGCCGATGAGTGGAAGCCTGATCCGCAGACAAAACAGGTCATCGACGGCTGGAATAAGCAA
CTTGATCAACTGGTGCAGCAGGTCATTACACAATCGCCCGTTGAACTGACCCGTGCGTATGGCATCTCTTCGCCGTTGGG
CAATCTTGCCGCTGACGCCTTGCTGCTGGCAGCAGGTAGGAGTACGCAGATGGCGTTAACCAACTCGGGCGGGATCCGGA
ATGAGATCCCCGCCGGGGCGGTGAGCATGGGCGCCGTTATCAGCACCTTCCCGTTTCCCAACGAGCTGGTCACCATGGAT
CTAACCGGGAAGCAGTTGCGTAGCCTGATGGAGCATGGCGCCGGGTTGAGCAACGGCGTGCTGCAGGTCTCCCGGGGACT
GGAAATGAAGTATGACAGCAGCAAACCTGTGGGCCAGCGCGTTGTGTTATTCAGGCTAAACGGTAAACCGATTGACGATA
CGACGGTTTACCATATTGTCACCAACAGCTTCCTTGCAGATGGCGGCGATGGTTTTGTCGCGTTTACCGAGGGGCAGGCG
CGTAATACCTCCGGCGGCTATTACGTTTCAAATGCGGTCGTAGACTATTTCAAAGCCGGGCAAAAGATAACGGATGAGCA
GCTTAGCGGGCAGCGCGTTGTCGATGTGAAGAAATAA

Upstream 100 bases:

>100_bases
TTTGTCCTGCTCAGGTTGCATACAACTGTGAATGCTCTCACATAAAAAAGTCTTTCAGAACGTAACTTAGCGGTTCTTTA
TCACTCTGAAAGGAACTCCG

Downstream 100 bases:

>100_bases
GTTTTTAAGGCCGGGCAGTCAGCCCGGCCTTAAGCGATCAGTGGGCCGCAAATACCGCCCTGGCGGCAAACAGGCCGTTG
AGGGCCGCCGGGAATCCGGC

Product: putative 5'-nucleotidase/2',3'-cyclic phosphodiesterase

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MKLKAMSAALLLTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIADGKRDIGGWANITTMVKQEKAKNNATWFFDAGDYFT
GPYISSLTKGKAIIDIMNTMSYDAVTIGNHEFDHGWDNTLLQLSQATFPIVQGNIFYQNSDKSFWDKPYTIIEKDGVKIG
VIGLHGVFAFNDTVSAATRVGIEARDEVKWLQHYIDELNGKVDLTVALIHEGVPARQSSQGGTDVRRALDKDIQTAGQVK
GLDVLITGHAHVGTPEPIKVGNTLILSTDSGGIDVGKLVLDYQEKPHQFTVKHFELKTLYADEWKPDPQTKQVIDGWNKQ
LDQLVQQVITQSPVELTRAYGISSPLGNLAADALLLAAGRSTQMALTNSGGIRNEIPAGAVSMGAVISTFPFPNELVTMD
LTGKQLRSLMEHGAGLSNGVLQVSRGLEMKYDSSKPVGQRVVLFRLNGKPIDDTTVYHIVTNSFLADGGDGFVAFTEGQA
RNTSGGYYVSNAVVDYFKAGQKITDEQLSGQRVVDVKK

Sequences:

>Translated_518_residues
MKLKAMSAALLLTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIADGKRDIGGWANITTMVKQEKAKNNATWFFDAGDYFT
GPYISSLTKGKAIIDIMNTMSYDAVTIGNHEFDHGWDNTLLQLSQATFPIVQGNIFYQNSDKSFWDKPYTIIEKDGVKIG
VIGLHGVFAFNDTVSAATRVGIEARDEVKWLQHYIDELNGKVDLTVALIHEGVPARQSSQGGTDVRRALDKDIQTAGQVK
GLDVLITGHAHVGTPEPIKVGNTLILSTDSGGIDVGKLVLDYQEKPHQFTVKHFELKTLYADEWKPDPQTKQVIDGWNKQ
LDQLVQQVITQSPVELTRAYGISSPLGNLAADALLLAAGRSTQMALTNSGGIRNEIPAGAVSMGAVISTFPFPNELVTMD
LTGKQLRSLMEHGAGLSNGVLQVSRGLEMKYDSSKPVGQRVVLFRLNGKPIDDTTVYHIVTNSFLADGGDGFVAFTEGQA
RNTSGGYYVSNAVVDYFKAGQKITDEQLSGQRVVDVKK
>Mature_518_residues
MKLKAMSAALLLTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIADGKRDIGGWANITTMVKQEKAKNNATWFFDAGDYFT
GPYISSLTKGKAIIDIMNTMSYDAVTIGNHEFDHGWDNTLLQLSQATFPIVQGNIFYQNSDKSFWDKPYTIIEKDGVKIG
VIGLHGVFAFNDTVSAATRVGIEARDEVKWLQHYIDELNGKVDLTVALIHEGVPARQSSQGGTDVRRALDKDIQTAGQVK
GLDVLITGHAHVGTPEPIKVGNTLILSTDSGGIDVGKLVLDYQEKPHQFTVKHFELKTLYADEWKPDPQTKQVIDGWNKQ
LDQLVQQVITQSPVELTRAYGISSPLGNLAADALLLAAGRSTQMALTNSGGIRNEIPAGAVSMGAVISTFPFPNELVTMD
LTGKQLRSLMEHGAGLSNGVLQVSRGLEMKYDSSKPVGQRVVLFRLNGKPIDDTTVYHIVTNSFLADGGDGFVAFTEGQA
RNTSGGYYVSNAVVDYFKAGQKITDEQLSGQRVVDVKK

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=533, Percent_Identity=26.8292682926829, Blast_Score=121, Evalue=2e-27,
Organism=Escherichia coli, GI1786687, Length=502, Percent_Identity=23.7051792828685, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790658, Length=577, Percent_Identity=22.0103986135182, Blast_Score=82, Evalue=9e-17,
Organism=Drosophila melanogaster, GI19922446, Length=554, Percent_Identity=24.187725631769, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24654424, Length=554, Percent_Identity=24.187725631769, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI221329836, Length=540, Percent_Identity=26.2962962962963, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI28573524, Length=558, Percent_Identity=24.0143369175627, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24641187, Length=513, Percent_Identity=25.7309941520468, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI19921980, Length=487, Percent_Identity=24.8459958932238, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI19922444, Length=554, Percent_Identity=24.9097472924188, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24652512, Length=487, Percent_Identity=24.8459958932238, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI161076508, Length=487, Percent_Identity=24.8459958932238, Blast_Score=122, Evalue=7e-28,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 56636; Mature: 56636

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKAMSAALLLTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIADGKRDIGGWANITTMV
CCCHHHHHHHHHHHHHHHHCCCEEEEEECCCEECCCCEECCEEECCCCCCCCHHHHHHHH
KQEKAKNNATWFFDAGDYFTGPYISSLTKGKAIIDIMNTMSYDAVTIGNHEFDHGWDNTL
HHHHCCCCCEEEEECCCCCCCHHHHHHCCCCHHEEHHHCCCCCEEEECCCCCCCCCCHHH
LQLSQATFPIVQGNIFYQNSDKSFWDKPYTIIEKDGVKIGVIGLHGVFAFNDTVSAATRV
HHHHCCCCEEEECEEEEECCCCCCCCCCCEEEECCCCEEEEEEEEEEEEECCHHHHHHHC
GIEARDEVKWLQHYIDELNGKVDLTVALIHEGVPARQSSQGGTDVRRALDKDIQTAGQVK
CCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
GLDVLITGHAHVGTPEPIKVGNTLILSTDSGGIDVGKLVLDYQEKPHQFTVKHFELKTLY
CEEEEEECCCCCCCCCCEECCCEEEEEECCCCCCHHHEEEECCCCCCEEEEEEEEEEEEE
ADEWKPDPQTKQVIDGWNKQLDQLVQQVITQSPVELTRAYGISSPLGNLAADALLLAAGR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHEECCC
STQMALTNSGGIRNEIPAGAVSMGAVISTFPFPNELVTMDLTGKQLRSLMEHGAGLSNGV
CCEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHCCCCCCCH
LQVSRGLEMKYDSSKPVGQRVVLFRLNGKPIDDTTVYHIVTNSFLADGGDGFVAFTEGQA
HHHHCCCEEEECCCCCCCCEEEEEEECCCCCCCCEEEEEEECCEECCCCCEEEEEECCCC
RNTSGGYYVSNAVVDYFKAGQKITDEQLSGQRVVDVKK
CCCCCCEEEHHHHHHHHHCCCCCCHHHCCCCEEEEECC
>Mature Secondary Structure
MKLKAMSAALLLTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIADGKRDIGGWANITTMV
CCCHHHHHHHHHHHHHHHHCCCEEEEEECCCEECCCCEECCEEECCCCCCCCHHHHHHHH
KQEKAKNNATWFFDAGDYFTGPYISSLTKGKAIIDIMNTMSYDAVTIGNHEFDHGWDNTL
HHHHCCCCCEEEEECCCCCCCHHHHHHCCCCHHEEHHHCCCCCEEEECCCCCCCCCCHHH
LQLSQATFPIVQGNIFYQNSDKSFWDKPYTIIEKDGVKIGVIGLHGVFAFNDTVSAATRV
HHHHCCCCEEEECEEEEECCCCCCCCCCCEEEECCCCEEEEEEEEEEEEECCHHHHHHHC
GIEARDEVKWLQHYIDELNGKVDLTVALIHEGVPARQSSQGGTDVRRALDKDIQTAGQVK
CCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
GLDVLITGHAHVGTPEPIKVGNTLILSTDSGGIDVGKLVLDYQEKPHQFTVKHFELKTLY
CEEEEEECCCCCCCCCCEECCCEEEEEECCCCCCHHHEEEECCCCCCEEEEEEEEEEEEE
ADEWKPDPQTKQVIDGWNKQLDQLVQQVITQSPVELTRAYGISSPLGNLAADALLLAAGR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHEECCC
STQMALTNSGGIRNEIPAGAVSMGAVISTFPFPNELVTMDLTGKQLRSLMEHGAGLSNGV
CCEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHCCCCCCCH
LQVSRGLEMKYDSSKPVGQRVVLFRLNGKPIDDTTVYHIVTNSFLADGGDGFVAFTEGQA
HHHHCCCEEEECCCCCCCCEEEEEEECCCCCCCCEEEEEEECCEECCCCCEEEEEECCCC
RNTSGGYYVSNAVVDYFKAGQKITDEQLSGQRVVDVKK
CCCCCCEEEHHHHHHHHHCCCCCCHHHCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]