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The map label for this gene is lacY [H]

Identifier: 238892312

GI number: 238892312

Start: 60537

End: 61922

Strand: Direct

Name: lacY [H]

Synonym: KP1_0063

Alternate gene names: 238892312

Gene position: 60537-61922 (Clockwise)

Preceding gene: 238892311

Following gene: 238892322

Centisome position: 1.15

GC content: 56.78

Gene sequence:

>1386_bases
GTGGCTGCAGCAGGCGGGGCTGGCGCTACCCATCCTCGACCCGGAAAGCGCCATTCTGATTGGTCTGCAACGCGTGTGAT
GGTATCGGGCTGCGCTGCGGCCCTTCTTGAGGAAAATATAATGAACCCGACCGTCTGTACCCACAAAAACAACCCCAACT
TCTGGATTTTCGGGCTGTTCTTCTTTCTCTACTTCTTCATCATGGCCACCTGCTTTCCGTTTCTGCCGATCTGGCTGTCG
GACGTCATCGGCCTGAATAAAACCGAAACCGGCCTCGTTTTCTCCAGCCTGTCGCTGTTCGCCATCTGTTTCCAGCCGAT
CCTCGGAGTGATCTCTGACAAGCTGGGGCTGAAAAAACACCTGATGTGGATCGTGACCGTTCTGCTGGTGCTGATTGCTC
CCTTCTTCCTTTATGTTTTCGCTCCGCTGCTGAAAACCAATATCTGGCTCGGGGCGCTGAGCGGCGGCGCCTATATCGGC
TTTGCATTCTCCGCAGGCGCCGGCGCGATGGAGGCCTATATCGAACGGGTAAGCCGCAACAGCGGGTTTGAATACGGCAA
GGCGCGCACCTTTGGCTGCCTGGGATGGGCGCTGTGCGCGACTACCGCCGGAATGCTGTTCAGCATTAATCCCGAATGGG
TGTTCTGGATGGGGTCAGCCGCCGCGCTGCTGCTGGTGGTGCTGGTGGCCATCGCCAAACCGCAGGCCAGTCAGTCCGCG
CAGGTGATGGATTCGCTCGGCGCCAATCGCCCGGCGATCGATTTAAAAACGGCGGTGCGCATGTTCCGCCAGCGTAAGAT
GTGGATGTTTATTCTGTATGTTATCGGCGTGGCCTGCGTGTATGACGTTTTCGATCAGCAGTTCGCCACCTTCTTCAAAT
CGTTCTTCGCGACCCCGGAAGCGGGCACCCGCGCCTTTGGTTTCGCCACCACCGCGGGAGAAATATGTAACGCCATCATT
ATGTTCAGCTCGCCGTGGATTATTAACCGTATCGGAGCGAAGAATACCCTGCTGATCGCCGGGATGGTGATGGCGGCAAG
AATGATTGGCTCTTCCTTCGCCACCACCGCCGCGGAAGTCGTAGCGTTGAAAATGCTCCATGCGCTGGAGGTGCCGTTCC
TGCTGGTGGGCGCCTTTAAATACATCACCGGCGTGTTTGACGTCCGGCTGTCGGCGACCATTTATCTGGTGGGCTTCCAG
TTCGCCAAGCAGGTGGCGGCGATCTTTCTCTCCGCTTTCGCCGGCAATATGTACGATCGGATCGGCTTTCAGGATACCTA
TATGATCCTCGGCGGTATTGCCCTGACCGTCACCTTAATCTCCGCGTTTACGTTAGCGGGTAAGATGAAAACCGAGCCGC
TGCGCGATAATGCAATGACCGTTTAA

Upstream 100 bases:

>100_bases
CGCTCCTCGATCATCCGAACATTCAGATCACCGGTGAGGGCGGCCACACCATGCGGAAGCTGCCGGCATGGATGACGACG
CCGCAGACGGTGAGCGGTGA

Downstream 100 bases:

>100_bases
TCGACCGGCCTCCCGTCTGGAGGGAGGCCAGTTATTTTTACGCCAGATAAAACACTTCTTTGAGCTCCGCGCTCAGCGGG
CTGTTGTCCGAGTTGCTGGG

Product: galactoside permease

Products: Proton [Cytoplasm]; lactose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MAAAGGAGATHPRPGKRHSDWSATRVMVSGCAAALLEENIMNPTVCTHKNNPNFWIFGLFFFLYFFIMATCFPFLPIWLS
DVIGLNKTETGLVFSSLSLFAICFQPILGVISDKLGLKKHLMWIVTVLLVLIAPFFLYVFAPLLKTNIWLGALSGGAYIG
FAFSAGAGAMEAYIERVSRNSGFEYGKARTFGCLGWALCATTAGMLFSINPEWVFWMGSAAALLLVVLVAIAKPQASQSA
QVMDSLGANRPAIDLKTAVRMFRQRKMWMFILYVIGVACVYDVFDQQFATFFKSFFATPEAGTRAFGFATTAGEICNAII
MFSSPWIINRIGAKNTLLIAGMVMAARMIGSSFATTAAEVVALKMLHALEVPFLLVGAFKYITGVFDVRLSATIYLVGFQ
FAKQVAAIFLSAFAGNMYDRIGFQDTYMILGGIALTVTLISAFTLAGKMKTEPLRDNAMTV

Sequences:

>Translated_461_residues
MAAAGGAGATHPRPGKRHSDWSATRVMVSGCAAALLEENIMNPTVCTHKNNPNFWIFGLFFFLYFFIMATCFPFLPIWLS
DVIGLNKTETGLVFSSLSLFAICFQPILGVISDKLGLKKHLMWIVTVLLVLIAPFFLYVFAPLLKTNIWLGALSGGAYIG
FAFSAGAGAMEAYIERVSRNSGFEYGKARTFGCLGWALCATTAGMLFSINPEWVFWMGSAAALLLVVLVAIAKPQASQSA
QVMDSLGANRPAIDLKTAVRMFRQRKMWMFILYVIGVACVYDVFDQQFATFFKSFFATPEAGTRAFGFATTAGEICNAII
MFSSPWIINRIGAKNTLLIAGMVMAARMIGSSFATTAAEVVALKMLHALEVPFLLVGAFKYITGVFDVRLSATIYLVGFQ
FAKQVAAIFLSAFAGNMYDRIGFQDTYMILGGIALTVTLISAFTLAGKMKTEPLRDNAMTV
>Mature_460_residues
AAAGGAGATHPRPGKRHSDWSATRVMVSGCAAALLEENIMNPTVCTHKNNPNFWIFGLFFFLYFFIMATCFPFLPIWLSD
VIGLNKTETGLVFSSLSLFAICFQPILGVISDKLGLKKHLMWIVTVLLVLIAPFFLYVFAPLLKTNIWLGALSGGAYIGF
AFSAGAGAMEAYIERVSRNSGFEYGKARTFGCLGWALCATTAGMLFSINPEWVFWMGSAAALLLVVLVAIAKPQASQSAQ
VMDSLGANRPAIDLKTAVRMFRQRKMWMFILYVIGVACVYDVFDQQFATFFKSFFATPEAGTRAFGFATTAGEICNAIIM
FSSPWIINRIGAKNTLLIAGMVMAARMIGSSFATTAAEVVALKMLHALEVPFLLVGAFKYITGVFDVRLSATIYLVGFQF
AKQVAAIFLSAFAGNMYDRIGFQDTYMILGGIALTVTLISAFTLAGKMKTEPLRDNAMTV

Specific function: Responsible for transport of raffinose into the cell, with the concomitant import of a proton (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacY/rafB permease family [H]

Homologues:

Organism=Escherichia coli, GI1786538, Length=395, Percent_Identity=58.9873417721519, Blast_Score=484, Evalue=1e-138,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022814
- InterPro:   IPR000576
- InterPro:   IPR018457
- InterPro:   IPR020846
- InterPro:   IPR016196 [H]

Pfam domain/function: PF01306 LacY_symp [H]

EC number: NA

Molecular weight: Translated: 50200; Mature: 50069

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: PS50850 MFS ; PS00896 LACY_1 ; PS00897 LACY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAAGGAGATHPRPGKRHSDWSATRVMVSGCAAALLEENIMNPTVCTHKNNPNFWIFGLF
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEHHHHH
FFLYFFIMATCFPFLPIWLSDVIGLNKTETGLVFSSLSLFAICFQPILGVISDKLGLKKH
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMWIVTVLLVLIAPFFLYVFAPLLKTNIWLGALSGGAYIGFAFSAGAGAMEAYIERVSRN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEEECCCCHHHHHHHHHHHCC
SGFEYGKARTFGCLGWALCATTAGMLFSINPEWVFWMGSAAALLLVVLVAIAKPQASQSA
CCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCEEEEECHHHHHHHHHHHHHCCCCCCHHH
QVMDSLGANRPAIDLKTAVRMFRQRKMWMFILYVIGVACVYDVFDQQFATFFKSFFATPE
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AGTRAFGFATTAGEICNAIIMFSSPWIINRIGAKNTLLIAGMVMAARMIGSSFATTAAEV
CCCCHHCCCCHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHH
VALKMLHALEVPFLLVGAFKYITGVFDVRLSATIYLVGFQFAKQVAAIFLSAFAGNMYDR
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHH
IGFQDTYMILGGIALTVTLISAFTLAGKMKTEPLRDNAMTV
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AAAGGAGATHPRPGKRHSDWSATRVMVSGCAAALLEENIMNPTVCTHKNNPNFWIFGLF
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEHHHHH
FFLYFFIMATCFPFLPIWLSDVIGLNKTETGLVFSSLSLFAICFQPILGVISDKLGLKKH
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMWIVTVLLVLIAPFFLYVFAPLLKTNIWLGALSGGAYIGFAFSAGAGAMEAYIERVSRN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEEECCCCHHHHHHHHHHHCC
SGFEYGKARTFGCLGWALCATTAGMLFSINPEWVFWMGSAAALLLVVLVAIAKPQASQSA
CCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCEEEEECHHHHHHHHHHHHHCCCCCCHHH
QVMDSLGANRPAIDLKTAVRMFRQRKMWMFILYVIGVACVYDVFDQQFATFFKSFFATPE
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AGTRAFGFATTAGEICNAIIMFSSPWIINRIGAKNTLLIAGMVMAARMIGSSFATTAAEV
CCCCHHCCCCHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHH
VALKMLHALEVPFLLVGAFKYITGVFDVRLSATIYLVGFQFAKQVAAIFLSAFAGNMYDR
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHH
IGFQDTYMILGGIALTVTLISAFTLAGKMKTEPLRDNAMTV
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; lactose [Periplasm] [C]

Specific reaction: Proton [Periplasm] + lactose [Periplasm] = Proton [Cytoplasm] + lactose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2556373 [H]