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The map label for this gene is exoA [H]

Identifier: 238028924

GI number: 238028924

Start: 3851604

End: 3852374

Strand: Reverse

Name: exoA [H]

Synonym: bglu_1g33890

Alternate gene names: 238028924

Gene position: 3852374-3851604 (Counterclockwise)

Preceding gene: 238028925

Following gene: 238028922

Centisome position: 98.61

GC content: 64.72

Gene sequence:

>771_bases
ATGCTGCGTGTCATTACGGCGAACCTGAACGGCATCCGCTCCGCCGCGAAGAAGGGCTTCTTCGACTGGCTGGGCGACCA
GCAGGCCGACTGCGTCTGCGTGCAGGAAATCAAGGTGTCGGCCGACGACCTGCCCGCCGAATTCGCGGCGCCGCACGGCT
TCACCGGCTATTTCCACCATGCCCAGAAGAAGGGCTACAGCGGCGCGGGGCTCTACACGCGCCGCGAACCCGACGACGTG
ATCATCGGCTACGGCAGCAGCGAGTTCGACGCCGAGGGGCGCTACGTCGAGGCGCGCTTCGGCAAGCTGTCGGTGGTGTC
GGTGTACGTGCCGTCGGGTTCGAGCGGCGACGAGCGCCAGCAGGCCAAGTACCGCTTCATGAACGAGTTCATGCCGCATC
TGGCGGAACTGAAGGCGACGCGCGAGGTGATCGTCTGCGGCGACGTGAACATCGTCCACAAGGAAATCGACATCCGCAAC
TGGAAGAGCAACCAGAAGAATTCCGGCTGCCTGCCCGAGGAGCGCGCGTGGCTGACCAAGCTGTTCGACGAGGTCGGCTA
CGTGGACGTGTTCCGCACGCTCGACCCGCGCCCCGACCAGTACACCTGGTGGAGCAACCGCGGCCAGGCCTACGCGAAGA
ACGTGGGGTGGCGGATCGACTACCAGATCGCCACGCCGGGCGTGGCGGGCACCGCGAAGCAGACCTCGATCTTCCGCGAC
ATCAAGTTCAGCGACCACGCACCGTTGACGGTGGATTACGACTATCAGTAA

Upstream 100 bases:

>100_bases
TATCCGCCCGCCAAGCCCGAGCTGGCGGGCGTGAAGATCGCGTGACGGCGCCGCGTGGGGCGCCATCCATCGAACCCGGA
ACACTGAACGGAAACACATC

Downstream 100 bases:

>100_bases
GCAGCGGCCGCCCGCCAGGTCGCAAAAAAACGCCGCCGGCATGACTCCCGGCGGCGTTTTTTTTCCTGCCGATGCCCCGG
CCATCGGCCGGGGCGGCGCG

Product: endonuclease/exonuclease/phosphatase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MLRVITANLNGIRSAAKKGFFDWLGDQQADCVCVQEIKVSADDLPAEFAAPHGFTGYFHHAQKKGYSGAGLYTRREPDDV
IIGYGSSEFDAEGRYVEARFGKLSVVSVYVPSGSSGDERQQAKYRFMNEFMPHLAELKATREVIVCGDVNIVHKEIDIRN
WKSNQKNSGCLPEERAWLTKLFDEVGYVDVFRTLDPRPDQYTWWSNRGQAYAKNVGWRIDYQIATPGVAGTAKQTSIFRD
IKFSDHAPLTVDYDYQ

Sequences:

>Translated_256_residues
MLRVITANLNGIRSAAKKGFFDWLGDQQADCVCVQEIKVSADDLPAEFAAPHGFTGYFHHAQKKGYSGAGLYTRREPDDV
IIGYGSSEFDAEGRYVEARFGKLSVVSVYVPSGSSGDERQQAKYRFMNEFMPHLAELKATREVIVCGDVNIVHKEIDIRN
WKSNQKNSGCLPEERAWLTKLFDEVGYVDVFRTLDPRPDQYTWWSNRGQAYAKNVGWRIDYQIATPGVAGTAKQTSIFRD
IKFSDHAPLTVDYDYQ
>Mature_256_residues
MLRVITANLNGIRSAAKKGFFDWLGDQQADCVCVQEIKVSADDLPAEFAAPHGFTGYFHHAQKKGYSGAGLYTRREPDDV
IIGYGSSEFDAEGRYVEARFGKLSVVSVYVPSGSSGDERQQAKYRFMNEFMPHLAELKATREVIVCGDVNIVHKEIDIRN
WKSNQKNSGCLPEERAWLTKLFDEVGYVDVFRTLDPRPDQYTWWSNRGQAYAKNVGWRIDYQIATPGVAGTAKQTSIFRD
IKFSDHAPLTVDYDYQ

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=253, Percent_Identity=36.7588932806324, Blast_Score=174, Evalue=6e-44,
Organism=Homo sapiens, GI18375503, Length=253, Percent_Identity=36.7588932806324, Blast_Score=174, Evalue=6e-44,
Organism=Homo sapiens, GI18375501, Length=253, Percent_Identity=36.7588932806324, Blast_Score=174, Evalue=6e-44,
Organism=Escherichia coli, GI1788046, Length=266, Percent_Identity=30.8270676691729, Blast_Score=113, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71989536, Length=257, Percent_Identity=29.9610894941634, Blast_Score=137, Evalue=5e-33,
Organism=Drosophila melanogaster, GI221330655, Length=251, Percent_Identity=33.8645418326693, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI17136678, Length=251, Percent_Identity=33.8645418326693, Blast_Score=148, Evalue=3e-36,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 28971; Mature: 28971

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRVITANLNGIRSAAKKGFFDWLGDQQADCVCVQEIKVSADDLPAEFAAPHGFTGYFHH
CEEEEEECCHHHHHHHHHCHHHHCCCCCCCEEEEEEEECCCCCCCHHHCCCCCCCHHHHH
AQKKGYSGAGLYTRREPDDVIIGYGSSEFDAEGRYVEARFGKLSVVSVYVPSGSSGDERQ
HHHCCCCCCCCEECCCCCCEEEECCCCCCCCCCCEEEEECCEEEEEEEEECCCCCCCHHH
QAKYRFMNEFMPHLAELKATREVIVCGDVNIVHKEIDIRNWKSNQKNSGCLPEERAWLTK
HHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHH
LFDEVGYVDVFRTLDPRPDQYTWWSNRGQAYAKNVGWRIDYQIATPGVAGTAKQTSIFRD
HHHHCCCEEHHHCCCCCCCCCEEECCCCCEEECCCCEEEEEEEECCCCCCCCHHHHHHHC
IKFSDHAPLTVDYDYQ
CCCCCCCCEEEECCCC
>Mature Secondary Structure
MLRVITANLNGIRSAAKKGFFDWLGDQQADCVCVQEIKVSADDLPAEFAAPHGFTGYFHH
CEEEEEECCHHHHHHHHHCHHHHCCCCCCCEEEEEEEECCCCCCCHHHCCCCCCCHHHHH
AQKKGYSGAGLYTRREPDDVIIGYGSSEFDAEGRYVEARFGKLSVVSVYVPSGSSGDERQ
HHHCCCCCCCCEECCCCCCEEEECCCCCCCCCCCEEEEECCEEEEEEEEECCCCCCCHHH
QAKYRFMNEFMPHLAELKATREVIVCGDVNIVHKEIDIRNWKSNQKNSGCLPEERAWLTK
HHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHH
LFDEVGYVDVFRTLDPRPDQYTWWSNRGQAYAKNVGWRIDYQIATPGVAGTAKQTSIFRD
HHHHCCCEEHHHCCCCCCCCCEEECCCCCEEECCCCEEEEEEEECCCCCCCCHHHHHHHC
IKFSDHAPLTVDYDYQ
CCCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]