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The map label for this gene is gacA [H]

Identifier: 238028878

GI number: 238028878

Start: 3802349

End: 3803002

Strand: Direct

Name: gacA [H]

Synonym: bglu_1g33430

Alternate gene names: 238028878

Gene position: 3802349-3803002 (Clockwise)

Preceding gene: 238028877

Following gene: 238028879

Centisome position: 97.33

GC content: 66.06

Gene sequence:

>654_bases
ATGGAATTGACCCTGCTGCTCGTCGACGATCACGCGATCGTGCGCCAGGGCATCGGCCAGCTGTTGATCGACCGCCGCGT
CGCGCGCGAGGTGGCCGAGGCCGAGTCCGGCGCCGAGGCGCTCGCGGCCGCGGACAAGGGCAGTTTCGACGTGATCCTGC
TCGACATCTCGCTGCCCGACATCAACGGCGTCGAGCTGCTCAAGCGCCTCAAGCGCAAGCTGCCGACGGTGCCGGTGCTG
ATGTTCTCGATGTACCGCGAGGACCAGTACGCGGTGCGGGCACTGAAGGCCGGCGCATCCGGCTATCTGTCGAAGGCGGT
CAACGCGACGCAGATGATCTCGGCGATCCAGCAGGTGGTGGCGGGACGCAAGTACGTGAGCCCGGCGCTCGCCGAGGCGC
TCGCCGAGTACGTCTCGTTCGAGAACGAGCCGATGCCGCACGAGAAGCTCTCGGACCGCGAATACCAGACGCTCTGCATG
CTCGCCTCGGGCAAGCGGCTGACCGACATCGCGCTCACGCTGTCGCTGTCGGTGAAGACGGTCAGCGTCTACCGCACGCG
GCTGCTCGAGAAGATGAAGCTGTCGAACAACGCCGAACTGACGTTCTACGCGATGAGCAACCGCCTGCTCGAAATGACGC
CGCCGTCGCCGTGA

Upstream 100 bases:

>100_bases
CGCGGGCGGCGGCACGCTGCTGCGCGCGAGCTTCGGGTGGGACGCGCTGCTCGCGGTGCCCGGCGCGCACCGGCGGGCTT
CGGCTTCCTGAGGCGCGGCG

Downstream 100 bases:

>100_bases
CGCCGCCGTCGCCGCCGGCCGGCGCCTGAACCTGCGGCGCCCGGACGCCGTCTGGCGTCCCCCCCTCGGCCGCGCTGCGG
CCTGCGCTGCCGCGTCGCGT

Product: LuxR family two component transcriptional regulator

Products: NA

Alternate protein names: Global activator [H]

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MELTLLLVDDHAIVRQGIGQLLIDRRVAREVAEAESGAEALAAADKGSFDVILLDISLPDINGVELLKRLKRKLPTVPVL
MFSMYREDQYAVRALKAGASGYLSKAVNATQMISAIQQVVAGRKYVSPALAEALAEYVSFENEPMPHEKLSDREYQTLCM
LASGKRLTDIALTLSLSVKTVSVYRTRLLEKMKLSNNAELTFYAMSNRLLEMTPPSP

Sequences:

>Translated_217_residues
MELTLLLVDDHAIVRQGIGQLLIDRRVAREVAEAESGAEALAAADKGSFDVILLDISLPDINGVELLKRLKRKLPTVPVL
MFSMYREDQYAVRALKAGASGYLSKAVNATQMISAIQQVVAGRKYVSPALAEALAEYVSFENEPMPHEKLSDREYQTLCM
LASGKRLTDIALTLSLSVKTVSVYRTRLLEKMKLSNNAELTFYAMSNRLLEMTPPSP
>Mature_217_residues
MELTLLLVDDHAIVRQGIGQLLIDRRVAREVAEAESGAEALAAADKGSFDVILLDISLPDINGVELLKRLKRKLPTVPVL
MFSMYREDQYAVRALKAGASGYLSKAVNATQMISAIQQVVAGRKYVSPALAEALAEYVSFENEPMPHEKLSDREYQTLCM
LASGKRLTDIALTLSLSVKTVSVYRTRLLEKMKLSNNAELTFYAMSNRLLEMTPPSP

Specific function: Positively controls the production of the autoinducer N- butyryl-homoserine lactone and the formation of the virulence factors pyocyanine, cyanide, and lipase [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=208, Percent_Identity=34.1346153846154, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1790102, Length=198, Percent_Identity=40.4040404040404, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1788521, Length=206, Percent_Identity=31.0679611650485, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1788712, Length=207, Percent_Identity=26.0869565217391, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1786747, Length=202, Percent_Identity=31.6831683168317, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787473, Length=183, Percent_Identity=32.7868852459016, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1786599, Length=152, Percent_Identity=28.2894736842105, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1788546, Length=208, Percent_Identity=25.4807692307692, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1788191, Length=120, Percent_Identity=31.6666666666667, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1790437, Length=119, Percent_Identity=33.6134453781513, Blast_Score=60, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23974; Mature: 23974

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELTLLLVDDHAIVRQGIGQLLIDRRVAREVAEAESGAEALAAADKGSFDVILLDISLPD
CEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEEEEEECCC
INGVELLKRLKRKLPTVPVLMFSMYREDQYAVRALKAGASGYLSKAVNATQMISAIQQVV
CCHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
AGRKYVSPALAEALAEYVSFENEPMPHEKLSDREYQTLCMLASGKRLTDIALTLSLSVKT
HCCHHCCHHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHCCCCEEEEEEEEEEHHHH
VSVYRTRLLEKMKLSNNAELTFYAMSNRLLEMTPPSP
HHHHHHHHHHHHHCCCCCCEEEEEECCCEEECCCCCC
>Mature Secondary Structure
MELTLLLVDDHAIVRQGIGQLLIDRRVAREVAEAESGAEALAAADKGSFDVILLDISLPD
CEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEEEEEECCC
INGVELLKRLKRKLPTVPVLMFSMYREDQYAVRALKAGASGYLSKAVNATQMISAIQQVV
CCHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
AGRKYVSPALAEALAEYVSFENEPMPHEKLSDREYQTLCMLASGKRLTDIALTLSLSVKT
HCCHHCCHHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHCCCCEEEEEEEEEEHHHH
VSVYRTRLLEKMKLSNNAELTFYAMSNRLLEMTPPSP
HHHHHHHHHHHHHCCCCCCEEEEEECCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9159518; 10984043 [H]