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The map label for this gene is flgJ [H]

Identifier: 238028844

GI number: 238028844

Start: 3768208

End: 3769197

Strand: Reverse

Name: flgJ [H]

Synonym: bglu_1g33090

Alternate gene names: 238028844

Gene position: 3769197-3768208 (Counterclockwise)

Preceding gene: 339905981

Following gene: 238028843

Centisome position: 96.49

GC content: 70.91

Gene sequence:

>990_bases
ATGGCCAATCTTCCGAATGCCGACCTCGCCGCCGGCACCGACCTTACCCAGCGCTTCGCGCTCGACGTGCAGGGCTTCGA
CCAGCTGCGCCGGCAGGCCGACGCCTCGCCCGGCGCCGGCATGAAGCAGGTCGCCACGCAGTTCGACGCGATGTTCTCGC
AGATGATGCTCAAGAGCATGCGCGATGCGACGCCGCAGGACGGCGTGTTCGACTCGAACACCTCGAAGCTCTACACCTCG
ATGCTCGACCAGCAGCTCTCGCAGCAGATGGCCTCGCGCGGCATCGGCGTGGCCGACGCGCTGATGAAGCAGCTGATGCG
CAACATGAATCCGGCGGCCGGCGCGGCCGCCGCCGGCGGCCCGGCGGGGGCGCTGGGCGCACTCGGCGGCACCGCCGGCA
ACGAGGGCGGCCTCGCCGCGGCCGGCGCGCTGGCCCAGGCGCTCGCCAACGCGCGCGGCAACAACCGGCTCAGCGGCGTT
GCCGGCTACACGGCCTCGAGCGCGCTGACGCCGCCCGTCAAGGGCAACGGCACCTCGCCCGACCAGGACGGCTTCGTCGA
CAAGCTGGCGGGCGCGGCGCAGGCGGCCAGCGCGGCCACCGGCATCCCGGCGCGCTTCATCGTCGGCCAGGCGGCGCTCG
AATCGGGCTGGGGCAAGCGCGAGATCCGCGGCCGCGACGGCAGCACCAGCTACAACGTGTTCGGCATCAAGGCGACCAAG
AACTGGACCGGGCGCACCGTGTCGGCCGTCACGACCGAGTACGTCGGCGGCCAGCCGCGCCGCGTGGTGGCGCAGTTCCG
CGCCTACGATTCCTACCAGCACGCGATGACCGACTACGCCACCATGCTCAAGAACAACCCGCGCTACGCGAACGTGGTGA
CCGGCTCGACCACCCCGGAAAGCTTCGCCAACGGCATGCAGCGCGCCGGTTACGCGACCGACCCGCACTACGCGAAGAAA
CTGATCTCGATCCTGCGCCAGATCGTCTGA

Upstream 100 bases:

>100_bases
AGGCGCTGAATTCTCTCGGCGCCACACCGGCCGACCTGATGTCGATCCTGCAGGCGATGAAAGCCGCTGGAGCGCTGCGG
GCCGACCTGGAGATCATCTG

Downstream 100 bases:

>100_bases
GGCCGGCGGCGCGGCGGCGCGCGATCGGTCGACGGGGCGGGCGGTGTCGTTTTTTACCCATTTTCAGGTATCCGGCATTT
CGAAATGATGTTTATCTGGA

Product: flagellar rod assembly protein/muramidase FlgJ

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MANLPNADLAAGTDLTQRFALDVQGFDQLRRQADASPGAGMKQVATQFDAMFSQMMLKSMRDATPQDGVFDSNTSKLYTS
MLDQQLSQQMASRGIGVADALMKQLMRNMNPAAGAAAAGGPAGALGALGGTAGNEGGLAAAGALAQALANARGNNRLSGV
AGYTASSALTPPVKGNGTSPDQDGFVDKLAGAAQAASAATGIPARFIVGQAALESGWGKREIRGRDGSTSYNVFGIKATK
NWTGRTVSAVTTEYVGGQPRRVVAQFRAYDSYQHAMTDYATMLKNNPRYANVVTGSTTPESFANGMQRAGYATDPHYAKK
LISILRQIV

Sequences:

>Translated_329_residues
MANLPNADLAAGTDLTQRFALDVQGFDQLRRQADASPGAGMKQVATQFDAMFSQMMLKSMRDATPQDGVFDSNTSKLYTS
MLDQQLSQQMASRGIGVADALMKQLMRNMNPAAGAAAAGGPAGALGALGGTAGNEGGLAAAGALAQALANARGNNRLSGV
AGYTASSALTPPVKGNGTSPDQDGFVDKLAGAAQAASAATGIPARFIVGQAALESGWGKREIRGRDGSTSYNVFGIKATK
NWTGRTVSAVTTEYVGGQPRRVVAQFRAYDSYQHAMTDYATMLKNNPRYANVVTGSTTPESFANGMQRAGYATDPHYAKK
LISILRQIV
>Mature_328_residues
ANLPNADLAAGTDLTQRFALDVQGFDQLRRQADASPGAGMKQVATQFDAMFSQMMLKSMRDATPQDGVFDSNTSKLYTSM
LDQQLSQQMASRGIGVADALMKQLMRNMNPAAGAAAAGGPAGALGALGGTAGNEGGLAAAGALAQALANARGNNRLSGVA
GYTASSALTPPVKGNGTSPDQDGFVDKLAGAAQAASAATGIPARFIVGQAALESGWGKREIRGRDGSTSYNVFGIKATKN
WTGRTVSAVTTEYVGGQPRRVVAQFRAYDSYQHAMTDYATMLKNNPRYANVVTGSTTPESFANGMQRAGYATDPHYAKKL
ISILRQIV

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: COG1705

COG function: function code NU; Muramidase (flagellum-specific)

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=310, Percent_Identity=40.9677419354839, Blast_Score=231, Evalue=4e-62,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR000423
- InterPro:   IPR019301
- InterPro:   IPR013338
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 34231; Mature: 34100

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANLPNADLAAGTDLTQRFALDVQGFDQLRRQADASPGAGMKQVATQFDAMFSQMMLKSM
CCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
RDATPQDGVFDSNTSKLYTSMLDQQLSQQMASRGIGVADALMKQLMRNMNPAAGAAAAGG
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCC
PAGALGALGGTAGNEGGLAAAGALAQALANARGNNRLSGVAGYTASSALTPPVKGNGTSP
CCHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DQDGFVDKLAGAAQAASAATGIPARFIVGQAALESGWGKREIRGRDGSTSYNVFGIKATK
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECC
NWTGRTVSAVTTEYVGGQPRRVVAQFRAYDSYQHAMTDYATMLKNNPRYANVVTGSTTPE
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHH
SFANGMQRAGYATDPHYAKKLISILRQIV
HHHHHHHHCCCCCCHHHHHHHHHHHHHHC
>Mature Secondary Structure 
ANLPNADLAAGTDLTQRFALDVQGFDQLRRQADASPGAGMKQVATQFDAMFSQMMLKSM
CCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
RDATPQDGVFDSNTSKLYTSMLDQQLSQQMASRGIGVADALMKQLMRNMNPAAGAAAAGG
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCC
PAGALGALGGTAGNEGGLAAAGALAQALANARGNNRLSGVAGYTASSALTPPVKGNGTSP
CCHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DQDGFVDKLAGAAQAASAATGIPARFIVGQAALESGWGKREIRGRDGSTSYNVFGIKATK
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECC
NWTGRTVSAVTTEYVGGQPRRVVAQFRAYDSYQHAMTDYATMLKNNPRYANVVTGSTTPE
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHH
SFANGMQRAGYATDPHYAKKLISILRQIV
HHHHHHHHCCCCCCHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]