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The map label for this gene is purR [H]

Identifier: 238028805

GI number: 238028805

Start: 3722119

End: 3723138

Strand: Direct

Name: purR [H]

Synonym: bglu_1g32690

Alternate gene names: 238028805

Gene position: 3722119-3723138 (Clockwise)

Preceding gene: 238028804

Following gene: 238028806

Centisome position: 95.28

GC content: 73.92

Gene sequence:

>1020_bases
ATGGGCACCACCATTCGCGATGTCGCGCGGGCGGCGAACGTTTCGATCGGCACCGTTTCGCGCGCGCTGAAGAACCAGCC
GGGCCTGTCCGAGGCCACCCGCGAGCGCGTGGTCGCGGTGGCCGCCGAGCTCGGCTACGATCCCGCGCAACTGCGCCCGC
GGATTCGCCGGCTCACGTTCCTGCTGCACCGCCAGCACAATAATTTCGCCGCCACGCCGTTCTTCTCGCACGTGCTGCAC
GGCGTGGAGGAGGCCTGCCGCGAGCGCGGCATCGTGCCGGCGCTGCTGACAGTCGGGCCGACCGACGACGTGCTGCGCCA
GATGCGCCCGCACGCGCCCGACGCGATCGCGGTGGCCGGCTTCATGGAGCCCGAGACGATCGACGCGCTCGCCGCCACCG
GCCGCCCGCTCGTGCTGATCGACATGTGGGCGCCGGGCCTGCGCTCGGTGAACATCGACAACGGCGCCGGCGCCTCGCTG
GCGATGCGGCACCTGTTCGCCGCCGGCCGCCGGCGCATCGCCTTCATCGGCGGCTCGCCCGCGCACCACAGCATCTCGCA
GCGCGCGCTCGGGTTCCGCCGCGCGTTCTTCGAGGCGGGGCTGCTGTTCGATCCGTCGCTGGAAGTGACGATCGACGCCG
GGCTCGACCCCGACACGGGCGCCGCGCGCGCGATGGAGCGCATCCTCGACGGCGGCGGCCCGCGCCCCGACGCCGTGTTC
GCGTTCAACGACGCGGCCGCGCTGGCCGCGATGCGGGTGTGCCTCGCACGCGGGCTGCGCGTGCCCGAGGACATCGCCCT
CGTCGGCTTCGACGACATCCCCGGCGCCGCGCACGCGGCCCCGCCGCTCACCACGCTGGCGGTCGACAAGGAAGCGCTCG
GCCGCCGCGGCGTGGAGCTGCTGCTCGCCGACGCGCCCGAGGAAACCGAAATTCGCCTGCCCGTGCGGCTGATCGAGCGC
GCGAGCAGTTCCGCCGCGGCGCATGCGCCGCGCCCCCTCACCCCGGTAATCGCCTCATGA

Upstream 100 bases:

>100_bases
AAATCCGGGTAATTACCAACTTTCCAGGCAGCCCGCGCATCATTTCATCGCGATTTTTTACTATACAATCGCCGACATCC
TAAACACCGGATCGGCCAGC

Downstream 100 bases:

>100_bases
CGAACCCAGCTTCGCCGACCGCGCCGGACACGCCGGTGCCGCACGTCGCGGACTTCCGCGACGCGGCCTTCCTGATGTCG
CACGTGCAGCACACGCTGCG

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MGTTIRDVARAANVSIGTVSRALKNQPGLSEATRERVVAVAAELGYDPAQLRPRIRRLTFLLHRQHNNFAATPFFSHVLH
GVEEACRERGIVPALLTVGPTDDVLRQMRPHAPDAIAVAGFMEPETIDALAATGRPLVLIDMWAPGLRSVNIDNGAGASL
AMRHLFAAGRRRIAFIGGSPAHHSISQRALGFRRAFFEAGLLFDPSLEVTIDAGLDPDTGAARAMERILDGGGPRPDAVF
AFNDAAALAAMRVCLARGLRVPEDIALVGFDDIPGAAHAAPPLTTLAVDKEALGRRGVELLLADAPEETEIRLPVRLIER
ASSSAAAHAPRPLTPVIAS

Sequences:

>Translated_339_residues
MGTTIRDVARAANVSIGTVSRALKNQPGLSEATRERVVAVAAELGYDPAQLRPRIRRLTFLLHRQHNNFAATPFFSHVLH
GVEEACRERGIVPALLTVGPTDDVLRQMRPHAPDAIAVAGFMEPETIDALAATGRPLVLIDMWAPGLRSVNIDNGAGASL
AMRHLFAAGRRRIAFIGGSPAHHSISQRALGFRRAFFEAGLLFDPSLEVTIDAGLDPDTGAARAMERILDGGGPRPDAVF
AFNDAAALAAMRVCLARGLRVPEDIALVGFDDIPGAAHAAPPLTTLAVDKEALGRRGVELLLADAPEETEIRLPVRLIER
ASSSAAAHAPRPLTPVIAS
>Mature_338_residues
GTTIRDVARAANVSIGTVSRALKNQPGLSEATRERVVAVAAELGYDPAQLRPRIRRLTFLLHRQHNNFAATPFFSHVLHG
VEEACRERGIVPALLTVGPTDDVLRQMRPHAPDAIAVAGFMEPETIDALAATGRPLVLIDMWAPGLRSVNIDNGAGASLA
MRHLFAAGRRRIAFIGGSPAHHSISQRALGFRRAFFEAGLLFDPSLEVTIDAGLDPDTGAARAMERILDGGGPRPDAVFA
FNDAAALAAMRVCLARGLRVPEDIALVGFDDIPGAAHAAPPLTTLAVDKEALGRRGVELLLADAPEETEIRLPVRLIERA
SSSAAAHAPRPLTPVIAS

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=318, Percent_Identity=32.7044025157233, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI1787948, Length=340, Percent_Identity=31.4705882352941, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1790194, Length=339, Percent_Identity=32.1533923303835, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1789202, Length=297, Percent_Identity=32.6599326599327, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1789456, Length=320, Percent_Identity=27.8125, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1788474, Length=331, Percent_Identity=29.3051359516616, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1786540, Length=355, Percent_Identity=27.887323943662, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1789068, Length=298, Percent_Identity=27.5167785234899, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1787580, Length=316, Percent_Identity=27.2151898734177, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI48994940, Length=315, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787906, Length=352, Percent_Identity=27.5568181818182, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1790689, Length=315, Percent_Identity=24.1269841269841, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790715, Length=316, Percent_Identity=23.4177215189873, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36031; Mature: 35900

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTTIRDVARAANVSIGTVSRALKNQPGLSEATRERVVAVAAELGYDPAQLRPRIRRLTF
CCCHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LLHRQHNNFAATPFFSHVLHGVEEACRERGIVPALLTVGPTDDVLRQMRPHAPDAIAVAG
HHHHCCCCEECCHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCCCCEEEEC
FMEPETIDALAATGRPLVLIDMWAPGLRSVNIDNGAGASLAMRHLFAAGRRRIAFIGGSP
CCCCHHHHHHHHCCCCEEEEEECCCCCCEEECCCCCCHHHHHHHHHHHCCCEEEEECCCC
AHHSISQRALGFRRAFFEAGLLFDPSLEVTIDAGLDPDTGAARAMERILDGGGPRPDAVF
CHHHHHHHHHHHHHHHHHCCCEECCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEE
AFNDAAALAAMRVCLARGLRVPEDIALVGFDDIPGAAHAAPPLTTLAVDKEALGRRGVEL
EECHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCEEEEECHHHHCCCCEEE
LLADAPEETEIRLPVRLIERASSSAAAHAPRPLTPVIAS
EEECCCCCCEEECHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GTTIRDVARAANVSIGTVSRALKNQPGLSEATRERVVAVAAELGYDPAQLRPRIRRLTF
CCHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LLHRQHNNFAATPFFSHVLHGVEEACRERGIVPALLTVGPTDDVLRQMRPHAPDAIAVAG
HHHHCCCCEECCHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCCCCEEEEC
FMEPETIDALAATGRPLVLIDMWAPGLRSVNIDNGAGASLAMRHLFAAGRRRIAFIGGSP
CCCCHHHHHHHHCCCCEEEEEECCCCCCEEECCCCCCHHHHHHHHHHHCCCEEEEECCCC
AHHSISQRALGFRRAFFEAGLLFDPSLEVTIDAGLDPDTGAARAMERILDGGGPRPDAVF
CHHHHHHHHHHHHHHHHHCCCEECCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEE
AFNDAAALAAMRVCLARGLRVPEDIALVGFDDIPGAAHAAPPLTTLAVDKEALGRRGVEL
EECHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCEEEEECHHHHCCCCEEE
LLADAPEETEIRLPVRLIERASSSAAAHAPRPLTPVIAS
EEECCCCCCEEECHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA