| Definition | Burkholderia glumae BGR1 chromosome chromosome 1, complete sequence. |
|---|---|
| Accession | NC_012724 |
| Length | 3,906,507 |
Click here to switch to the map view.
The map label for this gene is metH [H]
Identifier: 238028765
GI number: 238028765
Start: 3665919
End: 3668636
Strand: Direct
Name: metH [H]
Synonym: bglu_1g32290
Alternate gene names: 238028765
Gene position: 3665919-3668636 (Clockwise)
Preceding gene: 238028764
Following gene: 238028767
Centisome position: 93.84
GC content: 67.0
Gene sequence:
>2718_bases ATGACCGATCACACCATGCGCCTTGCCGGCCTCGAGCCGTTCAACGTCACCGAGGGCACGCTCTTCATCAACGTCGGCGA ACGCACCAACGTGACCGGTTCGAAGGCCTTCGCCCGCATGATCCTGAACGGCCAGTTCGACGAGGCGCTGGCGGTCGCGC GCCAGCAGGTCGAGAACGGCGCGCAGGTGATCGACATCAACATGGACGAGGCGATGCTCGATTCGAAGGCGGCCATGGTG CGCTTCCTGAACCTGATCGCCTCCGAGCCCGACATCGCGCGCGTGCCGATCATGATCGACTCGTCGAAGTGGGAGGTGAT CGAGGCGGGCCTCAAGTGCGTGCAGGGCAAGGCGATCGTCAACTCGATCTCGCTGAAGGAGGGCGAGGCGCAGTTCCGCC ATCATGCGCGGCTGATCCGCCGCTACGGCGCGGCGGCGGTGGTGATGGCGTTCGACGAGACCGGCCAGGCCGACACCTTC CAGCGCAAGACCGAGATCTGCGCGCGCTCGTACCGGATGCTGGTGGACGAATGCGGCTTCCCGCCCGAGGACATCATCTT CGATCCGAACATCTTCGCGGTGGCCACCGGCATCGAGGACCACAACAACTACGCGGTCGACTTCATCGAGGCGACGCGCT GGATCAAGCAGAACCTGCCGCACGCGAAGATCAGCGGCGGCGTGTCGAACGTGTCGTTCTCGTTTCGCGGCAACGACCCG GTGCGCGAGGCGATCCACACCGTGTTCCTCTACCACGCGATCCAGGCCGGCATGGACATGGGGATCGTCAACGCGGGCCA GCTCGGCGTCTACGCCGATCTCGACGCGGAACTGCGCGAGCGCGTCGAGGACGTGATCCTGAACCGCCGCGAGGACGCCA CCGACCGCCTGCTCGAAGTGGCCGACAAGTTCAAGACCGGCGCCGCGAAGAAGGAGGAGAGCCTCGAGTGGCGCAACCAG CCGGTCGAGAAGCGCCTCGCGCACGCGCTGGTGCACGGCATCACCACCTTCATCGTGGAGGACACGGAGGAGGCGCGCGT GGCGATCGACGCGGCGGGCGGGCGGCCGATCAACGTGATCGAGGGCCCGCTGATGGACGGCATGAACGTGGTCGGCGACC TGTTCGGCCAGGGCAAAATGTTCCTGCCGCAGGTGGTGAAGTCGGCGCGCGTGATGAAGCAGGCGGTCGCCCACCTGATC CCCTTCATCGAGGAGGAGAAGCGCCGCCTGGCCGAGGCCGGCGGCGACGTGCGCGCGAAGGGCAAGATCGTGATCGCGAC CGTCAAGGGCGACGTCCACGACATCGGCAAGAACATCGTGTCGGTGGTCCTTCAGTGCAACAACTTCGAAGTGGTCAACA TGGGCGTGATGGTGCCGTGCAACGAGATCCTCGCCAAGGCGAAGGTCGAGGGCGCCGACATCATCGGCCTGTCCGGCCTC ATCACGCCGAGCCTGGAGGAGATGGCCTACGTGGCCGCCGAGATGCAGCGCGACGACTACTTCCGCGTGAAGAAGATCCC GCTGCTGATCGGCGGTGCCACCACCTCGCGCGTGCATACGGCGGTGAAGATCGCGCCGAACTACGAAGGCCCGGTGGTGT ACGTGCCGGACGCCTCGCGCTCGGTGTCGGTGGCCTCCAGCCTGCTGTCGGACGAGGGCGCGACGCGCTACCTCGACGAG CTGAAGGGCGAATACGACCGGATCCGCGACCAGCACGCGAACAAGAAGAAGCAGCCGATGGTCACGCTGGCCGCCGCGCG CGCCAACAAGACGAAGATCGACTGGGCCGGCTACACGCCCGTCAAGCCGAAGTTCATCGGCCGGCGCGTGTTCAAGAACT ACGATCTGGCCGAACTCGCGCAGTATATCGACTGGGGCCCGTTCTTCCAGACCTGGGACCTGGCCGGCCCGTATCCGGCG ATCCTCAACGACGAGATCGTCGGCGAATCGGCGCGGCGCGTGTTCTCGGACGGCAAGTCGATGCTCTCGCGGCTGATCCA GGGCCGCTGGCTGACCGCCAACGGCGTGGTCTCGCTGCTGCCCGCCAACACGGTGAACGACGACGACATCGAGATCTACA CCGACGACACGCGCAGCGAGGTGCTGATGCGCTGGTGCAACCTGCGCCAGCAGAGCGTGCGCCCGGTGGTGGACGGGGTG ATGCGGCCGAACCGGTCGCTGGCCGATTTCATCGCCCCGAAGGACTCCGGCGTGGCCGACTACATCGGCATGTTCGCGGT CACGGCGGGGCTAGGCGTGGACGCCAAGGAAAAGCAGTTCGAGGCCGACCACGACGACTACAGCGCGATCATGCTGAAGG CGCTGGCGGACCGCTTCGCCGAGGCCTTCGCCGAGGCCCTGCACGCGCGCGTGCGGCGCGAGCTGTGGGGCTACGCGGCG GGCGAGACGCTCGACAACGACGCGCTGATCGCCGAGAAGTACGTGGGCATCCGCCCGGCGCCCGGTTATCCGGCCTGCCC CGACCACCTCGTCAAGCGCGCGATGTTCGATGCGCTGCGCGCCGACGAGCTCGGCATGAGCGTGACCGAGTCGCTGGCGA TGCTGCCGGCCGCGAGCGTGTCGGGCTTCTATATCGCGCATCCGGACAGCACCTATTTCTCGGTCGGCAAGATCGCCGAC GACCAGGTGGCCGACTTCGCCGCGCGCATGTCGCTGTCGGACGCCGACGCGCGCCGCGCGCTCGCGCCGCTGCTGTAG
Upstream 100 bases:
>100_bases CGCCGCTGGCCGTCGCACTACAGCGAAGCCGCCTGATCCACCACGCCGGCCGGCCGCCGCCGCGGGCGCCCCTACCGACA CGTCAACGACACACTCCGCC
Downstream 100 bases:
>100_bases GCGCACGCAAACGGCGCGGCGGCCGACAGCATAGTCGCCGTCGGCACAAAAAAAGCCCGCTTCCGGGAAGCGGGCTTTTT CACGGACGGCCGGCGCGCTG
Product: methionine synthase
Products: NA
Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]
Number of amino acids: Translated: 905; Mature: 904
Protein sequence:
>905_residues MTDHTMRLAGLEPFNVTEGTLFINVGERTNVTGSKAFARMILNGQFDEALAVARQQVENGAQVIDINMDEAMLDSKAAMV RFLNLIASEPDIARVPIMIDSSKWEVIEAGLKCVQGKAIVNSISLKEGEAQFRHHARLIRRYGAAAVVMAFDETGQADTF QRKTEICARSYRMLVDECGFPPEDIIFDPNIFAVATGIEDHNNYAVDFIEATRWIKQNLPHAKISGGVSNVSFSFRGNDP VREAIHTVFLYHAIQAGMDMGIVNAGQLGVYADLDAELRERVEDVILNRREDATDRLLEVADKFKTGAAKKEESLEWRNQ PVEKRLAHALVHGITTFIVEDTEEARVAIDAAGGRPINVIEGPLMDGMNVVGDLFGQGKMFLPQVVKSARVMKQAVAHLI PFIEEEKRRLAEAGGDVRAKGKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVPCNEILAKAKVEGADIIGLSGL ITPSLEEMAYVAAEMQRDDYFRVKKIPLLIGGATTSRVHTAVKIAPNYEGPVVYVPDASRSVSVASSLLSDEGATRYLDE LKGEYDRIRDQHANKKKQPMVTLAAARANKTKIDWAGYTPVKPKFIGRRVFKNYDLAELAQYIDWGPFFQTWDLAGPYPA ILNDEIVGESARRVFSDGKSMLSRLIQGRWLTANGVVSLLPANTVNDDDIEIYTDDTRSEVLMRWCNLRQQSVRPVVDGV MRPNRSLADFIAPKDSGVADYIGMFAVTAGLGVDAKEKQFEADHDDYSAIMLKALADRFAEAFAEALHARVRRELWGYAA GETLDNDALIAEKYVGIRPAPGYPACPDHLVKRAMFDALRADELGMSVTESLAMLPAASVSGFYIAHPDSTYFSVGKIAD DQVADFAARMSLSDADARRALAPLL
Sequences:
>Translated_905_residues MTDHTMRLAGLEPFNVTEGTLFINVGERTNVTGSKAFARMILNGQFDEALAVARQQVENGAQVIDINMDEAMLDSKAAMV RFLNLIASEPDIARVPIMIDSSKWEVIEAGLKCVQGKAIVNSISLKEGEAQFRHHARLIRRYGAAAVVMAFDETGQADTF QRKTEICARSYRMLVDECGFPPEDIIFDPNIFAVATGIEDHNNYAVDFIEATRWIKQNLPHAKISGGVSNVSFSFRGNDP VREAIHTVFLYHAIQAGMDMGIVNAGQLGVYADLDAELRERVEDVILNRREDATDRLLEVADKFKTGAAKKEESLEWRNQ PVEKRLAHALVHGITTFIVEDTEEARVAIDAAGGRPINVIEGPLMDGMNVVGDLFGQGKMFLPQVVKSARVMKQAVAHLI PFIEEEKRRLAEAGGDVRAKGKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVPCNEILAKAKVEGADIIGLSGL ITPSLEEMAYVAAEMQRDDYFRVKKIPLLIGGATTSRVHTAVKIAPNYEGPVVYVPDASRSVSVASSLLSDEGATRYLDE LKGEYDRIRDQHANKKKQPMVTLAAARANKTKIDWAGYTPVKPKFIGRRVFKNYDLAELAQYIDWGPFFQTWDLAGPYPA ILNDEIVGESARRVFSDGKSMLSRLIQGRWLTANGVVSLLPANTVNDDDIEIYTDDTRSEVLMRWCNLRQQSVRPVVDGV MRPNRSLADFIAPKDSGVADYIGMFAVTAGLGVDAKEKQFEADHDDYSAIMLKALADRFAEAFAEALHARVRRELWGYAA GETLDNDALIAEKYVGIRPAPGYPACPDHLVKRAMFDALRADELGMSVTESLAMLPAASVSGFYIAHPDSTYFSVGKIAD DQVADFAARMSLSDADARRALAPLL >Mature_904_residues TDHTMRLAGLEPFNVTEGTLFINVGERTNVTGSKAFARMILNGQFDEALAVARQQVENGAQVIDINMDEAMLDSKAAMVR FLNLIASEPDIARVPIMIDSSKWEVIEAGLKCVQGKAIVNSISLKEGEAQFRHHARLIRRYGAAAVVMAFDETGQADTFQ RKTEICARSYRMLVDECGFPPEDIIFDPNIFAVATGIEDHNNYAVDFIEATRWIKQNLPHAKISGGVSNVSFSFRGNDPV REAIHTVFLYHAIQAGMDMGIVNAGQLGVYADLDAELRERVEDVILNRREDATDRLLEVADKFKTGAAKKEESLEWRNQP VEKRLAHALVHGITTFIVEDTEEARVAIDAAGGRPINVIEGPLMDGMNVVGDLFGQGKMFLPQVVKSARVMKQAVAHLIP FIEEEKRRLAEAGGDVRAKGKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVPCNEILAKAKVEGADIIGLSGLI TPSLEEMAYVAAEMQRDDYFRVKKIPLLIGGATTSRVHTAVKIAPNYEGPVVYVPDASRSVSVASSLLSDEGATRYLDEL KGEYDRIRDQHANKKKQPMVTLAAARANKTKIDWAGYTPVKPKFIGRRVFKNYDLAELAQYIDWGPFFQTWDLAGPYPAI LNDEIVGESARRVFSDGKSMLSRLIQGRWLTANGVVSLLPANTVNDDDIEIYTDDTRSEVLMRWCNLRQQSVRPVVDGVM RPNRSLADFIAPKDSGVADYIGMFAVTAGLGVDAKEKQFEADHDDYSAIMLKALADRFAEAFAEALHARVRRELWGYAAG ETLDNDALIAEKYVGIRPAPGYPACPDHLVKRAMFDALRADELGMSVTESLAMLPAASVSGFYIAHPDSTYFSVGKIADD QVADFAARMSLSDADARRALAPLL
Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]
COG id: COG1410
COG function: function code E; Methionine synthase I, cobalamin-binding domain
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 pterin-binding domain [H]
Homologues:
Organism=Homo sapiens, GI169790923, Length=915, Percent_Identity=54.7540983606557, Blast_Score=971, Evalue=0.0, Organism=Escherichia coli, GI1790450, Length=904, Percent_Identity=62.8318584070796, Blast_Score=1097, Evalue=0.0, Organism=Caenorhabditis elegans, GI17535405, Length=912, Percent_Identity=54.4956140350877, Blast_Score=942, Evalue=0.0,
Paralogues:
None
Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR006158 - InterPro: IPR011005 - InterPro: IPR003759 - InterPro: IPR011822 - InterPro: IPR000489 - InterPro: IPR003726 - InterPro: IPR004223 [H]
Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]
EC number: =2.1.1.13 [H]
Molecular weight: Translated: 99593; Mature: 99462
Theoretical pI: Translated: 5.19; Mature: 5.19
Prosite motif: PS50974 ADOMET_ACTIVATION ; PS50972 PTERIN_BINDING
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.8 %Cys (Translated Protein) 3.2 %Met (Translated Protein) 4.0 %Cys+Met (Translated Protein) 0.8 %Cys (Mature Protein) 3.1 %Met (Mature Protein) 3.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTDHTMRLAGLEPFNVTEGTLFINVGERTNVTGSKAFARMILNGQFDEALAVARQQVENG CCCCCEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCC AQVIDINMDEAMLDSKAAMVRFLNLIASEPDIARVPIMIDSSKWEVIEAGLKCVQGKAIV CEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHCCHHHH NSISLKEGEAQFRHHARLIRRYGAAAVVMAFDETGQADTFQRKTEICARSYRMLVDECGF HHCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCC PPEDIIFDPNIFAVATGIEDHNNYAVDFIEATRWIKQNLPHAKISGGVSNVSFSFRGNDP CCHHEEECCCEEEEEECCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCH VREAIHTVFLYHAIQAGMDMGIVNAGQLGVYADLDAELRERVEDVILNRREDATDRLLEV HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCCCHHHHHHHH ADKFKTGAAKKEESLEWRNQPVEKRLAHALVHGITTFIVEDTEEARVAIDAAGGRPINVI HHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHEEECCCCCEEEEECCCCCEEEEE EGPLMDGMNVVGDLFGQGKMFLPQVVKSARVMKQAVAHLIPFIEEEKRRLAEAGGDVRAK CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEC GKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVPCNEILAKAKVEGADIIGLSGL CEEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEEECCHHHHHHHHHCCCCCEEEECCC ITPSLEEMAYVAAEMQRDDYFRVKKIPLLIGGATTSRVHTAVKIAPNYEGPVVYVPDASR CCCCHHHHHHHHHHHCCCCCEEEEECEEEEECCCHHHEEEEEEECCCCCCCEEECCCCCC SVSVASSLLSDEGATRYLDELKGEYDRIRDQHANKKKQPMVTLAAARANKTKIDWAGYTP HHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEECCCCC VKPKFIGRRVFKNYDLAELAQYIDWGPFFQTWDLAGPYPAILNDEIVGESARRVFSDGKS CCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHCCHHHHCHHHHHHHHHHHH MLSRLIQGRWLTANGVVSLLPANTVNDDDIEIYTDDTRSEVLMRWCNLRQQSVRPVVDGV HHHHHHCCCEEECCCEEEEECCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH MRPNRSLADFIAPKDSGVADYIGMFAVTAGLGVDAKEKQFEADHDDYSAIMLKALADRFA HCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHH EAFAEALHARVRRELWGYAAGETLDNDALIAEKYVGIRPAPGYPACPDHLVKRAMFDALR HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH ADELGMSVTESLAMLPAASVSGFYIAHPDSTYFSVGKIADDQVADFAARMSLSDADARRA HHHCCHHHHHHHHHCCCCCCCCEEEECCCCCEEEECCCCCHHHHHHHHHHCCCHHHHHHH LAPLL HCCCC >Mature Secondary Structure TDHTMRLAGLEPFNVTEGTLFINVGERTNVTGSKAFARMILNGQFDEALAVARQQVENG CCCCEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCC AQVIDINMDEAMLDSKAAMVRFLNLIASEPDIARVPIMIDSSKWEVIEAGLKCVQGKAIV CEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHCCHHHH NSISLKEGEAQFRHHARLIRRYGAAAVVMAFDETGQADTFQRKTEICARSYRMLVDECGF HHCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCC PPEDIIFDPNIFAVATGIEDHNNYAVDFIEATRWIKQNLPHAKISGGVSNVSFSFRGNDP CCHHEEECCCEEEEEECCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCH VREAIHTVFLYHAIQAGMDMGIVNAGQLGVYADLDAELRERVEDVILNRREDATDRLLEV HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCCCHHHHHHHH ADKFKTGAAKKEESLEWRNQPVEKRLAHALVHGITTFIVEDTEEARVAIDAAGGRPINVI HHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHEEECCCCCEEEEECCCCCEEEEE EGPLMDGMNVVGDLFGQGKMFLPQVVKSARVMKQAVAHLIPFIEEEKRRLAEAGGDVRAK CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEC GKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVPCNEILAKAKVEGADIIGLSGL CEEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEEECCHHHHHHHHHCCCCCEEEECCC ITPSLEEMAYVAAEMQRDDYFRVKKIPLLIGGATTSRVHTAVKIAPNYEGPVVYVPDASR CCCCHHHHHHHHHHHCCCCCEEEEECEEEEECCCHHHEEEEEEECCCCCCCEEECCCCCC SVSVASSLLSDEGATRYLDELKGEYDRIRDQHANKKKQPMVTLAAARANKTKIDWAGYTP HHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEECCCCC VKPKFIGRRVFKNYDLAELAQYIDWGPFFQTWDLAGPYPAILNDEIVGESARRVFSDGKS CCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHCCHHHHCHHHHHHHHHHHH MLSRLIQGRWLTANGVVSLLPANTVNDDDIEIYTDDTRSEVLMRWCNLRQQSVRPVVDGV HHHHHHCCCEEECCCEEEEECCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH MRPNRSLADFIAPKDSGVADYIGMFAVTAGLGVDAKEKQFEADHDDYSAIMLKALADRFA HCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHH EAFAEALHARVRRELWGYAAGETLDNDALIAEKYVGIRPAPGYPACPDHLVKRAMFDALR HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH ADELGMSVTESLAMLPAASVSGFYIAHPDSTYFSVGKIADDQVADFAARMSLSDADARRA HHHCCHHHHHHHHHCCCCCCCCEEEECCCCCEEEECCCCCHHHHHHHHHHCCCHHHHHHH LAPLL HCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 12620739 [H]