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The map label for this gene is metH [H]

Identifier: 238028764

GI number: 238028764

Start: 3664790

End: 3665854

Strand: Direct

Name: metH [H]

Synonym: bglu_1g32280

Alternate gene names: 238028764

Gene position: 3664790-3665854 (Clockwise)

Preceding gene: 238028763

Following gene: 238028765

Centisome position: 93.81

GC content: 68.83

Gene sequence:

>1065_bases
ATGTCGTCATCCCCGTCCGTCCGCCCTGCCCTTGCCGCGTCGTACACGCGCGGCGCCGACCTGCCCGCGCTGCTCGAGCG
CAGGATCCTGATCCTCGACGGCGCGATGGGCACGATGATCCAGCGCTACAAGTTCGACGAGGCCGCCTATCGCGGCGCAC
GCTTCGCCGACTTCCCGCGCGACATCAAGGGCAACAACGAGCTGCTGTCGCTGACCCAGCCCGACACGATCCGCGAGATC
CACGACCAGTACTTCGCGGCGGGCGCCGACATCGTCGAGACCAACACCTTCGGCGCGACCGCCATCGCCCAGGCCGACTA
CGGGATGGAGCACCTGGTGGCCGAGATGAACCTCGCATCGGCGAAGCTCGCGCGCGAGTCGGCGCAGCAATACTCGACGC
CGGACAAGCCGCGCTTCGTGGCCGGCGCGATCGGACCGACGCCGAAGACGGCCAGCATCTCGCCCGACGTCAACGACCCG
GGCGCGCGCAACGTCACCTATGACGAACTGCGCGATTCGTATTACGAACAGGCGAAGGCGCTGCTCGACGGCGGCGTGGA
CCTGTTCCTGGTCGAGACGATCTTCGACACGCTGAACGCGAAGGCCGCGCTGTTCGCGCTCGACCAGCTGTTCGAGGACA
CCGGCGAGCGCCTGCCGATCATGATCTCGGGCACCGTGACCGACGCGTCGGGCCGCATCCTCTCGGGCCAGACCGTCGAG
GCGTTCTGGAATTCGCTGCGCCACGCACGCCCGCTCACCTTCGGCCTGAACTGCGCGCTCGGCGCGGCGCTGATGCGCCC
GTACATCGCCGAGCTCGCCAAGCTCTGCGACACCTACGTGTCGTGCTACCCGAACGCGGGCCTGCCCAACCCGATGGCCG
AGACCGGCTTCGACGAAACGCCCGAGGTCACCTCGGGGCTGCTCAGGGAATTCGCGCAGGCCGGCCTCGTGAACCTCGCC
GGCGGCTGCTGCGGCACCACCCCCGAGCACATCGCCGAGATCGCCAAGGCACTGGCCGACGTGAAGCCGCGCCGCTGGCC
GTCGCACTACAGCGAAGCCGCCTGA

Upstream 100 bases:

>100_bases
CCGCCCGGCCCGCCGCGCGGGGGTTTTCGTTGGCGGGCACCCGACGTGCTCGTCCGGCAACCGCTACAATGTGGCTTTCC
GCCATTGCCGTTGCTCAGCC

Downstream 100 bases:

>100_bases
TCCACCACGCCGGCCGGCCGCCGCCGCGGGCGCCCCTACCGACACGTCAACGACACACTCCGCCATGACCGATCACACCA
TGCGCCTTGCCGGCCTCGAG

Product: cobalamin-dependent methionine synthase I

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MSSSPSVRPALAASYTRGADLPALLERRILILDGAMGTMIQRYKFDEAAYRGARFADFPRDIKGNNELLSLTQPDTIREI
HDQYFAAGADIVETNTFGATAIAQADYGMEHLVAEMNLASAKLARESAQQYSTPDKPRFVAGAIGPTPKTASISPDVNDP
GARNVTYDELRDSYYEQAKALLDGGVDLFLVETIFDTLNAKAALFALDQLFEDTGERLPIMISGTVTDASGRILSGQTVE
AFWNSLRHARPLTFGLNCALGAALMRPYIAELAKLCDTYVSCYPNAGLPNPMAETGFDETPEVTSGLLREFAQAGLVNLA
GGCCGTTPEHIAEIAKALADVKPRRWPSHYSEAA

Sequences:

>Translated_354_residues
MSSSPSVRPALAASYTRGADLPALLERRILILDGAMGTMIQRYKFDEAAYRGARFADFPRDIKGNNELLSLTQPDTIREI
HDQYFAAGADIVETNTFGATAIAQADYGMEHLVAEMNLASAKLARESAQQYSTPDKPRFVAGAIGPTPKTASISPDVNDP
GARNVTYDELRDSYYEQAKALLDGGVDLFLVETIFDTLNAKAALFALDQLFEDTGERLPIMISGTVTDASGRILSGQTVE
AFWNSLRHARPLTFGLNCALGAALMRPYIAELAKLCDTYVSCYPNAGLPNPMAETGFDETPEVTSGLLREFAQAGLVNLA
GGCCGTTPEHIAEIAKALADVKPRRWPSHYSEAA
>Mature_353_residues
SSSPSVRPALAASYTRGADLPALLERRILILDGAMGTMIQRYKFDEAAYRGARFADFPRDIKGNNELLSLTQPDTIREIH
DQYFAAGADIVETNTFGATAIAQADYGMEHLVAEMNLASAKLARESAQQYSTPDKPRFVAGAIGPTPKTASISPDVNDPG
ARNVTYDELRDSYYEQAKALLDGGVDLFLVETIFDTLNAKAALFALDQLFEDTGERLPIMISGTVTDASGRILSGQTVEA
FWNSLRHARPLTFGLNCALGAALMRPYIAELAKLCDTYVSCYPNAGLPNPMAETGFDETPEVTSGLLREFAQAGLVNLAG
GCCGTTPEHIAEIAKALADVKPRRWPSHYSEAA

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG0646

COG function: function code E; Methionine synthase I (cobalamin-dependent), methyltransferase domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=330, Percent_Identity=57.5757575757576, Blast_Score=394, Evalue=1e-110,
Organism=Escherichia coli, GI1790450, Length=329, Percent_Identity=59.5744680851064, Blast_Score=396, Evalue=1e-112,
Organism=Escherichia coli, GI1786456, Length=326, Percent_Identity=26.9938650306748, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17535405, Length=331, Percent_Identity=53.1722054380665, Blast_Score=357, Evalue=7e-99,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 38224; Mature: 38093

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSPSVRPALAASYTRGADLPALLERRILILDGAMGTMIQRYKFDEAAYRGARFADFPR
CCCCCCCCHHHHHHHCCCCCCHHHHHCCEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCC
DIKGNNELLSLTQPDTIREIHDQYFAAGADIVETNTFGATAIAQADYGMEHLVAEMNLAS
CCCCCCCEEECCCCHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHCCHHHHHHHHHHHH
AKLARESAQQYSTPDKPRFVAGAIGPTPKTASISPDVNDPGARNVTYDELRDSYYEQAKA
HHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LLDGGVDLFLVETIFDTLNAKAALFALDQLFEDTGERLPIMISGTVTDASGRILSGQTVE
HHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEECCCHHH
AFWNSLRHARPLTFGLNCALGAALMRPYIAELAKLCDTYVSCYPNAGLPNPMAETGFDET
HHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCC
PEVTSGLLREFAQAGLVNLAGGCCGTTPEHIAEIAKALADVKPRRWPSHYSEAA
HHHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SSSPSVRPALAASYTRGADLPALLERRILILDGAMGTMIQRYKFDEAAYRGARFADFPR
CCCCCCCHHHHHHHCCCCCCHHHHHCCEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCC
DIKGNNELLSLTQPDTIREIHDQYFAAGADIVETNTFGATAIAQADYGMEHLVAEMNLAS
CCCCCCCEEECCCCHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHCCHHHHHHHHHHHH
AKLARESAQQYSTPDKPRFVAGAIGPTPKTASISPDVNDPGARNVTYDELRDSYYEQAKA
HHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LLDGGVDLFLVETIFDTLNAKAALFALDQLFEDTGERLPIMISGTVTDASGRILSGQTVE
HHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEECCCHHH
AFWNSLRHARPLTFGLNCALGAALMRPYIAELAKLCDTYVSCYPNAGLPNPMAETGFDET
HHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCC
PEVTSGLLREFAQAGLVNLAGGCCGTTPEHIAEIAKALADVKPRRWPSHYSEAA
HHHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]