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The map label for this gene is lipB [H]

Identifier: 238028740

GI number: 238028740

Start: 3640690

End: 3641442

Strand: Reverse

Name: lipB [H]

Synonym: bglu_1g32040

Alternate gene names: 238028740

Gene position: 3641442-3640690 (Counterclockwise)

Preceding gene: 238028741

Following gene: 238028739

Centisome position: 93.21

GC content: 69.19

Gene sequence:

>753_bases
ATGTCAGCGCCGCCGGTTTCCATTCCGTCCCCCATCTCCACCGAGCCTGGCAGCGCGGTTGGCTCCGAGGCCGCCGCGCT
GCCGCCGATCGCCGTGCGGTGGCTGGGCTCGGTCCCCTACGAGCCGACCTTCGAGGCGATGCGCGCGTTCACCGATGCGC
GCTCGCCCGACACGCCCGACGAGATCTGGCTCGTCGAACATCCGCCCGTGTTCACGCTCGGCCAGGCCGGCAACCCTTCG
CACCTGCTCGTCGCCGACAGCGGCGTGCCGCTCGTGAAGGTCGATCGCGGCGGGCAGATCACCTATCACGGCCCGGGCCA
GATCGTCGCCTACCTGCTGGTGGACCTGCGGCGCCGCAAGCTGATGGTGCGCGGCATGGTCGAGCGCATCGAGCAGGCCG
TGATCGAAACCCTCGCGGCGTATAATCTCGCGTCGGTCCGCAAGGCGGGCGCGCCCGGTATCTATGTGGAATCGGGGCCG
CATGCCGGCGCGAAGATCGCCGCCCTCGGGCTCAAGATCCGCAACGGCTGCAGCTATCACGGCCTGAGCCTCAACGTGCG
GATGGACCTTCGCCCGTTTCTCGCGATCAATCCGTGCGGCTACGCGGGACTCGAAACGGTCGACATGGCGACCGTCGGCG
TGAGCGCCGACTGGCGCGACGTGGCCGGCACGCTCGTGCGCCGTCTGACCGCCAACCTCGACGGCGCCATCGCCGCCGCT
TTGCCGCAGGCACTGGAACATTCGAATGACTGA

Upstream 100 bases:

>100_bases
TGCTCGCGCGGCTCGCCAACTGGCTGGCACGCTTTCCCGACGGCTGGCGCACGGTCTGAGCGGCGCGCGGCGCGTGCCGC
TTTTCGCTAAACTAGCGTCC

Downstream 100 bases:

>100_bases
CGTAACCGCAACCCCCGCATCGGCCGATCCGGTCGCCGCCTACGATCCGACCTCGAAGCAGAAGGCCCAGGCGAAGACGG
CCCGCATTCCGATCAAGATC

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MSAPPVSIPSPISTEPGSAVGSEAAALPPIAVRWLGSVPYEPTFEAMRAFTDARSPDTPDEIWLVEHPPVFTLGQAGNPS
HLLVADSGVPLVKVDRGGQITYHGPGQIVAYLLVDLRRRKLMVRGMVERIEQAVIETLAAYNLASVRKAGAPGIYVESGP
HAGAKIAALGLKIRNGCSYHGLSLNVRMDLRPFLAINPCGYAGLETVDMATVGVSADWRDVAGTLVRRLTANLDGAIAAA
LPQALEHSND

Sequences:

>Translated_250_residues
MSAPPVSIPSPISTEPGSAVGSEAAALPPIAVRWLGSVPYEPTFEAMRAFTDARSPDTPDEIWLVEHPPVFTLGQAGNPS
HLLVADSGVPLVKVDRGGQITYHGPGQIVAYLLVDLRRRKLMVRGMVERIEQAVIETLAAYNLASVRKAGAPGIYVESGP
HAGAKIAALGLKIRNGCSYHGLSLNVRMDLRPFLAINPCGYAGLETVDMATVGVSADWRDVAGTLVRRLTANLDGAIAAA
LPQALEHSND
>Mature_249_residues
SAPPVSIPSPISTEPGSAVGSEAAALPPIAVRWLGSVPYEPTFEAMRAFTDARSPDTPDEIWLVEHPPVFTLGQAGNPSH
LLVADSGVPLVKVDRGGQITYHGPGQIVAYLLVDLRRRKLMVRGMVERIEQAVIETLAAYNLASVRKAGAPGIYVESGPH
AGAKIAALGLKIRNGCSYHGLSLNVRMDLRPFLAINPCGYAGLETVDMATVGVSADWRDVAGTLVRRLTANLDGAIAAAL
PQALEHSND

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family [H]

Homologues:

Organism=Escherichia coli, GI87081767, Length=206, Percent_Identity=51.4563106796116, Blast_Score=209, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6323268, Length=202, Percent_Identity=28.2178217821782, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21355483, Length=144, Percent_Identity=34.0277777777778, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 26417; Mature: 26285

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01313 LIPB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPPVSIPSPISTEPGSAVGSEAAALPPIAVRWLGSVPYEPTFEAMRAFTDARSPDTPD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCC
EIWLVEHPPVFTLGQAGNPSHLLVADSGVPLVKVDRGGQITYHGPGQIVAYLLVDLRRRK
CEEEEECCCEEEECCCCCCCEEEEECCCCCEEEECCCCEEEEECCHHHHHHHHHHHHHHH
LMVRGMVERIEQAVIETLAAYNLASVRKAGAPGIYVESGPHAGAKIAALGLKIRNGCSYH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEEECCCCCCC
GLSLNVRMDLRPFLAINPCGYAGLETVDMATVGVSADWRDVAGTLVRRLTANLDGAIAAA
CEEEEEEECCCCEEEECCCCCCCCCEEEHEEECCCCCHHHHHHHHHHHHHHCCCCHHHHH
LPQALEHSND
HHHHHHCCCC
>Mature Secondary Structure 
SAPPVSIPSPISTEPGSAVGSEAAALPPIAVRWLGSVPYEPTFEAMRAFTDARSPDTPD
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCC
EIWLVEHPPVFTLGQAGNPSHLLVADSGVPLVKVDRGGQITYHGPGQIVAYLLVDLRRRK
CEEEEECCCEEEECCCCCCCEEEEECCCCCEEEECCCCEEEEECCHHHHHHHHHHHHHHH
LMVRGMVERIEQAVIETLAAYNLASVRKAGAPGIYVESGPHAGAKIAALGLKIRNGCSYH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEEECCCCCCC
GLSLNVRMDLRPFLAINPCGYAGLETVDMATVGVSADWRDVAGTLVRRLTANLDGAIAAA
CEEEEEEECCCCEEEECCCCCCCCCEEEHEEECCCCCHHHHHHHHHHHHHHCCCCHHHHH
LPQALEHSND
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA