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The map label for this gene is lipA [H]

Identifier: 238028739

GI number: 238028739

Start: 3639711

End: 3640697

Strand: Reverse

Name: lipA [H]

Synonym: bglu_1g32030

Alternate gene names: 238028739

Gene position: 3640697-3639711 (Counterclockwise)

Preceding gene: 238028740

Following gene: 238028738

Centisome position: 93.2

GC content: 66.46

Gene sequence:

>987_bases
ATGACTGACGTAACCGCAACCCCCGCATCGGCCGATCCGGTCGCCGCCTACGATCCGACCTCGAAGCAGAAGGCCCAGGC
GAAGACGGCCCGCATTCCGATCAAGATCGTGCCGATCGAGAAGCTCAAGAAGCCCGAGTGGATCCGCGTGAAGGCGGCCA
CCGGCAGCTCGCGCTTCAACGAGATCAAGACGATCCTGCGCGAGCACAAGCTGCACACCGTCTGCGAGGAAGCGAGCTGC
CCAAACATCGGCGAGTGTTTCGGCAAAGGCACCGCGACCTTCATGATCATGGGCGACAAGTGCACGCGCCGCTGCCCGTT
CTGCGACGTCGGCCACGGCCGCCCCGATCCGCTCGATCCGCAGGAGCCGGCCAACCTCGCGCGCACCATCGGCGCGCTGC
GCCTGAAGTACGTGGTGATCACGAGCGTGGACCGCGACGACCTGCGCGACGGCGGCGCCGGCCACTTCGTCGAGTGCATC
CGCCAGGTGCGCGAGCAGTCGCCCGAGACGCGCATCGAGATCCTCACGCCCGACTTCCGCGGCCGTCTCGATCGCGCGAT
CGGCATCCTGAACGCCGCGCCGCCCGACGTGATGAACCACAACCTCGAAACGGTGCCGCGCCTCTACAAGGAAGCGCGCC
CGGGCTCGGACTACGCGCATTCGCTGAAGCTGCTGAAGGATTTCAAGGCGCTGCACCCGGAAGTCGCGACCAAGTCGGGG
CTGATGGTCGGCCTGGGCGAGACGCCCGAGGAAATCCTGCAGGTGATGCGCGACCTGCGCGAGCACGATGTCGACATGCT
GACGATCGGCCAGTACCTGCAGCCGTCCGAGCACCACCTGCCGGTGCGCGAGTACGTGCATCCGGACGTCTTCAAGATGT
ACGAGGAAGAGGCCTACAAGATGGGCTTCACGCACGCGGCCGTGGGCGCGATGGTGCGTTCGAGCTACCACGCCGATCTG
CAGGCGCATGGGGCGGGCGTGGTCTGA

Upstream 100 bases:

>100_bases
GCCGACTGGCGCGACGTGGCCGGCACGCTCGTGCGCCGTCTGACCGCCAACCTCGACGGCGCCATCGCCGCCGCTTTGCC
GCAGGCACTGGAACATTCGA

Downstream 100 bases:

>100_bases
AAGCCCGCCGGCGGCCGCTGCGCCGCCCGACAGCACGACACGAAAGCCCGCCTTCCGGCGGGCTTTCGCTCTTTTTGGCC
CGCTGGTCGCGGGCCGCCGT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MTDVTATPASADPVAAYDPTSKQKAQAKTARIPIKIVPIEKLKKPEWIRVKAATGSSRFNEIKTILREHKLHTVCEEASC
PNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDPQEPANLARTIGALRLKYVVITSVDRDDLRDGGAGHFVECI
RQVREQSPETRIEILTPDFRGRLDRAIGILNAAPPDVMNHNLETVPRLYKEARPGSDYAHSLKLLKDFKALHPEVATKSG
LMVGLGETPEEILQVMRDLREHDVDMLTIGQYLQPSEHHLPVREYVHPDVFKMYEEEAYKMGFTHAAVGAMVRSSYHADL
QAHGAGVV

Sequences:

>Translated_328_residues
MTDVTATPASADPVAAYDPTSKQKAQAKTARIPIKIVPIEKLKKPEWIRVKAATGSSRFNEIKTILREHKLHTVCEEASC
PNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDPQEPANLARTIGALRLKYVVITSVDRDDLRDGGAGHFVECI
RQVREQSPETRIEILTPDFRGRLDRAIGILNAAPPDVMNHNLETVPRLYKEARPGSDYAHSLKLLKDFKALHPEVATKSG
LMVGLGETPEEILQVMRDLREHDVDMLTIGQYLQPSEHHLPVREYVHPDVFKMYEEEAYKMGFTHAAVGAMVRSSYHADL
QAHGAGVV
>Mature_327_residues
TDVTATPASADPVAAYDPTSKQKAQAKTARIPIKIVPIEKLKKPEWIRVKAATGSSRFNEIKTILREHKLHTVCEEASCP
NIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDPQEPANLARTIGALRLKYVVITSVDRDDLRDGGAGHFVECIR
QVREQSPETRIEILTPDFRGRLDRAIGILNAAPPDVMNHNLETVPRLYKEARPGSDYAHSLKLLKDFKALHPEVATKSGL
MVGLGETPEEILQVMRDLREHDVDMLTIGQYLQPSEHHLPVREYVHPDVFKMYEEEAYKMGFTHAAVGAMVRSSYHADLQ
AHGAGVV

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=286, Percent_Identity=47.9020979020979, Blast_Score=273, Evalue=2e-73,
Organism=Homo sapiens, GI37577164, Length=250, Percent_Identity=48.4, Blast_Score=238, Evalue=7e-63,
Organism=Escherichia coli, GI1786846, Length=309, Percent_Identity=63.7540453074434, Blast_Score=415, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI32564533, Length=254, Percent_Identity=46.0629921259843, Blast_Score=231, Evalue=5e-61,
Organism=Saccharomyces cerevisiae, GI6324770, Length=286, Percent_Identity=45.8041958041958, Blast_Score=253, Evalue=3e-68,
Organism=Drosophila melanogaster, GI221513272, Length=286, Percent_Identity=47.2027972027972, Blast_Score=261, Evalue=4e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 36481; Mature: 36350

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDVTATPASADPVAAYDPTSKQKAQAKTARIPIKIVPIEKLKKPEWIRVKAATGSSRFN
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEECHHHHCCCCEEEEEECCCCHHHH
EIKTILREHKLHTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDP
HHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEEECCHHHCCCCCCCCCCCCCCCCCC
QEPANLARTIGALRLKYVVITSVDRDDLRDGGAGHFVECIRQVREQSPETRIEILTPDFR
CCHHHHHHHHHHHHEEEEEEEECCHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCHHH
GRLDRAIGILNAAPPDVMNHNLETVPRLYKEARPGSDYAHSLKLLKDFKALHPEVATKSG
HHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHCCCC
LMVGLGETPEEILQVMRDLREHDVDMLTIGQYLQPSEHHLPVREYVHPDVFKMYEEEAYK
CEEECCCCHHHHHHHHHHHHHCCCCEEEHHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHH
MGFTHAAVGAMVRSSYHADLQAHGAGVV
HCCHHHHHHHHHHHHHHCCHHCCCCCCC
>Mature Secondary Structure 
TDVTATPASADPVAAYDPTSKQKAQAKTARIPIKIVPIEKLKKPEWIRVKAATGSSRFN
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEECHHHHCCCCEEEEEECCCCHHHH
EIKTILREHKLHTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDP
HHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEEECCHHHCCCCCCCCCCCCCCCCCC
QEPANLARTIGALRLKYVVITSVDRDDLRDGGAGHFVECIRQVREQSPETRIEILTPDFR
CCHHHHHHHHHHHHEEEEEEEECCHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCHHH
GRLDRAIGILNAAPPDVMNHNLETVPRLYKEARPGSDYAHSLKLLKDFKALHPEVATKSG
HHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHCCCC
LMVGLGETPEEILQVMRDLREHDVDMLTIGQYLQPSEHHLPVREYVHPDVFKMYEEEAYK
CEEECCCCHHHHHHHHHHHHHCCCCEEEHHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHH
MGFTHAAVGAMVRSSYHADLQAHGAGVV
HCCHHHHHHHHHHHHHHCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA