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The map label for this gene is nagE [H]

Identifier: 238028685

GI number: 238028685

Start: 3578595

End: 3580376

Strand: Reverse

Name: nagE [H]

Synonym: bglu_1g31490

Alternate gene names: 238028685

Gene position: 3580376-3578595 (Counterclockwise)

Preceding gene: 238028686

Following gene: 238028680

Centisome position: 91.65

GC content: 69.36

Gene sequence:

>1782_bases
ATGGACGGGAATCCGTTTCTGAAAATACAGAGCCTGGGACGGGCGCTGATGTTGCCGATCGCGGTATTGCCCGTGGCGGG
CATCCTGCTGCGGCTGGGCCAGTCCGATGTGCTCAACATCAAGATGATCGCCGACGCGGGCGGCGCGGTGTTCGACAACC
TGCCGCTCCTGTTCGCGATCGGCGTGGCCGTCGGTTTCGCGAAGGACAACAACGGCGTCGCGGCGCTCGCGGGTGCGATC
GGCTACCTGATCGAAACCGCGATCATGAAGGACATCGACCCGAAGCTGAACATGGGCGTGCTGTCCGGGATCATCGCGGG
CATCGTGGCGGGACTGCTCTATAACCGCTACAAGGACATCAAGCTGCCGGACTATCTGGCGTTCTTCGGCGGCAAGCGCT
TCGTGCCGATCGTGACCGGCCTCGTCTGCGTGGTGCTCGGGATCGTGTTCGGCTACATCTGGGGGCCGATCCAGAACGTG
ATCGACGCGGCCGGCCACTGGCTGACCACGGCCGGTGCGATCGGCGCGTTCGTATACGGATTCCTGAACCGGCTGCTGCT
CGTCACCGGGCTGCACCACATCATCAACTCGCTCGCCTGGTTCGTGTTCGGCACGTTCACGCCGCCGGGCGGCGGCGCGC
CGGTAACGGGCGACCTGCACCGCTTCTTCGCGGGCGATCCGACCGCGGGCGGCTTCATGGCGGGCTTTTTCCCGATCATG
ATGTTCGGTCTGCCGGCCGCATGCCTGGCGATGTTCCACGAGGCGCCGAAGGCGCGCCGCGCGGTGGTGGGCGGCCTGCT
GTTCTCGATGGCGCTGACCTCGTTCCTGACCGGCGTGACCGAGCCGATCGAATTCAGCTTCATGTTCCTCGCGCCGGTGC
TGTACGTGATCCATGCGGTGCTGACGGGGCTTTCGCTCGCGATCTGTCAGCTGCTTGGCGTGAAGCTCGGCTTCACGTTT
TCGGCCGGCGCGATCGACTACGTGCTGAACTACGGGCTCTCGACGAAGGGCTGGATCGCAATCCCGCTCGGCCTTGCCTA
CGCGGTCGCCTACTACGCGCTGTTCCGCTTCTTCATCCGCAAGTTCAACATGGCCACGCCGGGCCGCGAGCCCGCCGGCG
ACGCGCCGCTGGCCGCGGACAGCCTCGGCGGCCCGGCCGGCGCGGCCGGCGGTTTCGCCGCGGCGCAGGTCGCGGGCGAT
GCGGCGGCGCCGCGCGCGCTGCGCTATATCGCGGCGCTCGGCGGTGCAGCGAACCTGACGACGGTCGATGCCTGCACGAC
GCGACTGCGTCTGTCGGTAGTCGATCCGGAGCAGGTGTCCGAGGCGAACCTGAGGGCGATCGGCGCGCGCGGCGTGCTCA
AGCGCGGCGGCAGCAGCGTGCAGGTGATCATCGGGCCTGAGGCGGACCTGATCGCCGACGAAATCCGCAGCGCGATCGGC
AGCGGCGAGGCCCCGGCGGCGGCCCCGGCTGCCACGCCGGCAGCCGCAGCGGCAGCACCGGGCGGCGAGGCCGGTCCGCT
CGATCCTGATCCGATGCGCTGGCTCGCCGTGTTCGGCGGCGCGGCCAACGTGGCGTCGCTCGACGCGGTCGCCGCCACGC
GGCTGCGCGTGGTGGTGCGCGATCCGTCGGCCGTCGATCGCGACCGGCTCGCGAGCGTGGATGTCGCCTGGGTGTCGACC
GACACGTTCCACATCATCTGCGGCAGCTCGGCCGCGCGTTACGCGCAGCAAATGTCGGCGCGCCTGCCGCCGGCCGGCGG
GGCGGCCGAGCAGCCCGCGTGA

Upstream 100 bases:

>100_bases
CCGCAGCAGTGAGCGGTCGCGCAGCCGGGTTCGCGTCGTGGGTTTCCCCACGCAGCATCAACACATCAACAACGAAAGAC
GAGACGATTGGGAGATCTGA

Downstream 100 bases:

>100_bases
GCGCGGCGCGGCGAACGCCGCGCACCCTGGCAAAACGGCGCCCGAGGGCGCCGTTTTTTTTGTGATGAAGACGACGGCGC
GGGCAGGGCGCGCCGCCGCC

Product: phosphotransferase system, IIbc component

Products: NA

Alternate protein names: EIICBA-Nag; EII-Nag; N-acetylglucosamine permease IIC component; PTS system N-acetylglucosamine-specific EIIC component; N-acetylglucosamine-specific phosphotransferase enzyme IIB component; PTS system N-acetylglucosamine-specific EIIB component; N-acetylglucosamine-specific phosphotransferase enzyme IIA component; PTS system N-acetylglucosamine-specific EIIA component [H]

Number of amino acids: Translated: 593; Mature: 593

Protein sequence:

>593_residues
MDGNPFLKIQSLGRALMLPIAVLPVAGILLRLGQSDVLNIKMIADAGGAVFDNLPLLFAIGVAVGFAKDNNGVAALAGAI
GYLIETAIMKDIDPKLNMGVLSGIIAGIVAGLLYNRYKDIKLPDYLAFFGGKRFVPIVTGLVCVVLGIVFGYIWGPIQNV
IDAAGHWLTTAGAIGAFVYGFLNRLLLVTGLHHIINSLAWFVFGTFTPPGGGAPVTGDLHRFFAGDPTAGGFMAGFFPIM
MFGLPAACLAMFHEAPKARRAVVGGLLFSMALTSFLTGVTEPIEFSFMFLAPVLYVIHAVLTGLSLAICQLLGVKLGFTF
SAGAIDYVLNYGLSTKGWIAIPLGLAYAVAYYALFRFFIRKFNMATPGREPAGDAPLAADSLGGPAGAAGGFAAAQVAGD
AAAPRALRYIAALGGAANLTTVDACTTRLRLSVVDPEQVSEANLRAIGARGVLKRGGSSVQVIIGPEADLIADEIRSAIG
SGEAPAAAPAATPAAAAAAPGGEAGPLDPDPMRWLAVFGGAANVASLDAVAATRLRVVVRDPSAVDRDRLASVDVAWVST
DTFHIICGSSAARYAQQMSARLPPAGGAAEQPA

Sequences:

>Translated_593_residues
MDGNPFLKIQSLGRALMLPIAVLPVAGILLRLGQSDVLNIKMIADAGGAVFDNLPLLFAIGVAVGFAKDNNGVAALAGAI
GYLIETAIMKDIDPKLNMGVLSGIIAGIVAGLLYNRYKDIKLPDYLAFFGGKRFVPIVTGLVCVVLGIVFGYIWGPIQNV
IDAAGHWLTTAGAIGAFVYGFLNRLLLVTGLHHIINSLAWFVFGTFTPPGGGAPVTGDLHRFFAGDPTAGGFMAGFFPIM
MFGLPAACLAMFHEAPKARRAVVGGLLFSMALTSFLTGVTEPIEFSFMFLAPVLYVIHAVLTGLSLAICQLLGVKLGFTF
SAGAIDYVLNYGLSTKGWIAIPLGLAYAVAYYALFRFFIRKFNMATPGREPAGDAPLAADSLGGPAGAAGGFAAAQVAGD
AAAPRALRYIAALGGAANLTTVDACTTRLRLSVVDPEQVSEANLRAIGARGVLKRGGSSVQVIIGPEADLIADEIRSAIG
SGEAPAAAPAATPAAAAAAPGGEAGPLDPDPMRWLAVFGGAANVASLDAVAATRLRVVVRDPSAVDRDRLASVDVAWVST
DTFHIICGSSAARYAQQMSARLPPAGGAAEQPA
>Mature_593_residues
MDGNPFLKIQSLGRALMLPIAVLPVAGILLRLGQSDVLNIKMIADAGGAVFDNLPLLFAIGVAVGFAKDNNGVAALAGAI
GYLIETAIMKDIDPKLNMGVLSGIIAGIVAGLLYNRYKDIKLPDYLAFFGGKRFVPIVTGLVCVVLGIVFGYIWGPIQNV
IDAAGHWLTTAGAIGAFVYGFLNRLLLVTGLHHIINSLAWFVFGTFTPPGGGAPVTGDLHRFFAGDPTAGGFMAGFFPIM
MFGLPAACLAMFHEAPKARRAVVGGLLFSMALTSFLTGVTEPIEFSFMFLAPVLYVIHAVLTGLSLAICQLLGVKLGFTF
SAGAIDYVLNYGLSTKGWIAIPLGLAYAVAYYALFRFFIRKFNMATPGREPAGDAPLAADSLGGPAGAAGGFAAAQVAGD
AAAPRALRYIAALGGAANLTTVDACTTRLRLSVVDPEQVSEANLRAIGARGVLKRGGSSVQVIIGPEADLIADEIRSAIG
SGEAPAAAPAATPAAAAAAPGGEAGPLDPDPMRWLAVFGGAANVASLDAVAATRLRVVVRDPSAVDRDRLASVDVAWVST
DTFHIICGSSAARYAQQMSARLPPAGGAAEQPA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786894, Length=476, Percent_Identity=52.9411764705882, Blast_Score=467, Evalue=1e-133,
Organism=Escherichia coli, GI1787343, Length=499, Percent_Identity=43.2865731462926, Blast_Score=378, Evalue=1e-106,
Organism=Escherichia coli, GI1787908, Length=531, Percent_Identity=36.9114877589454, Blast_Score=276, Evalue=2e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001127
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010974 [H]

Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 61126; Mature: 61126

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGNPFLKIQSLGRALMLPIAVLPVAGILLRLGQSDVLNIKMIADAGGAVFDNLPLLFAI
CCCCCEEEHHHHCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHCCHHHHHH
GVAVGFAKDNNGVAALAGAIGYLIETAIMKDIDPKLNMGVLSGIIAGIVAGLLYNRYKDI
HHHHEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
KLPDYLAFFGGKRFVPIVTGLVCVVLGIVFGYIWGPIQNVIDAAGHWLTTAGAIGAFVYG
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
FLNRLLLVTGLHHIINSLAWFVFGTFTPPGGGAPVTGDLHRFFAGDPTAGGFMAGFFPIM
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHH
MFGLPAACLAMFHEAPKARRAVVGGLLFSMALTSFLTGVTEPIEFSFMFLAPVLYVIHAV
HHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LTGLSLAICQLLGVKLGFTFSAGAIDYVLNYGLSTKGWIAIPLGLAYAVAYYALFRFFIR
HHHHHHHHHHHHHHHHCCEECCHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHH
KFNMATPGREPAGDAPLAADSLGGPAGAAGGFAAAQVAGDAAAPRALRYIAALGGAANLT
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCH
TVDACTTRLRLSVVDPEQVSEANLRAIGARGVLKRGGSSVQVIIGPEADLIADEIRSAIG
HHHHHHHHHEEEECCHHHHCCCHHHHHCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHC
SGEAPAAAPAATPAAAAAAPGGEAGPLDPDPMRWLAVFGGAANVASLDAVAATRLRVVVR
CCCCCCCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHEEEEEE
DPSAVDRDRLASVDVAWVSTDTFHIICGSSAARYAQQMSARLPPAGGAAEQPA
CCCCCCHHHHCCEEEEEEECCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MDGNPFLKIQSLGRALMLPIAVLPVAGILLRLGQSDVLNIKMIADAGGAVFDNLPLLFAI
CCCCCEEEHHHHCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHCCHHHHHH
GVAVGFAKDNNGVAALAGAIGYLIETAIMKDIDPKLNMGVLSGIIAGIVAGLLYNRYKDI
HHHHEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
KLPDYLAFFGGKRFVPIVTGLVCVVLGIVFGYIWGPIQNVIDAAGHWLTTAGAIGAFVYG
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
FLNRLLLVTGLHHIINSLAWFVFGTFTPPGGGAPVTGDLHRFFAGDPTAGGFMAGFFPIM
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHH
MFGLPAACLAMFHEAPKARRAVVGGLLFSMALTSFLTGVTEPIEFSFMFLAPVLYVIHAV
HHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LTGLSLAICQLLGVKLGFTFSAGAIDYVLNYGLSTKGWIAIPLGLAYAVAYYALFRFFIR
HHHHHHHHHHHHHHHHCCEECCHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHH
KFNMATPGREPAGDAPLAADSLGGPAGAAGGFAAAQVAGDAAAPRALRYIAALGGAANLT
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCH
TVDACTTRLRLSVVDPEQVSEANLRAIGARGVLKRGGSSVQVIIGPEADLIADEIRSAIG
HHHHHHHHHEEEECCHHHHCCCHHHHHCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHC
SGEAPAAAPAATPAAAAAAPGGEAGPLDPDPMRWLAVFGGAANVASLDAVAATRLRVVVR
CCCCCCCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHEEEEEE
DPSAVDRDRLASVDVAWVSTDTFHIICGSSAARYAQQMSARLPPAGGAAEQPA
CCCCCCHHHHCCEEEEEEECCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1745234 [H]