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The map label for this gene is leuA [H]

Identifier: 238028678

GI number: 238028678

Start: 3570682

End: 3572331

Strand: Direct

Name: leuA [H]

Synonym: bglu_1g31420

Alternate gene names: 238028678

Gene position: 3570682-3572331 (Clockwise)

Preceding gene: 238028677

Following gene: 238028681

Centisome position: 91.4

GC content: 68.79

Gene sequence:

>1650_bases
ATGCAGCGCAACCCCGCCGACAAGTACCGCCCATTCGAACCCGTTCGCCTGAACGGCCGCAAATGGCCATCCCGCACGAT
CACGCAGGCGCCGATCTGGATGTCCACCGACCTGCGCGACGGCAACCAGTCGCTGATCGAGCCGATGCGCATCGAGCAGA
AGCTCGAATTCTTCGAGATGCTCGTCGCGATCGGCTTCAAGGAGATCGAGGTCGGCTTCCCGTCGGCCTCGCAGACCGAT
TTCGACTTCGTGCGCAAGCTGATCGACGAGCGGCGGATCCCGGAAGACGTGACGATCGAGGTGCTGGTGCAGTCGCGCGA
GGACTTGATTGCTCGCACCTTCGAGGCGCTCGAAGGCGCGCCGCGCGCGATCGTCCATCTTTACAACGCGATCGCGCCGT
CGTTTCGCCAGATCGTGTTCGGTCTCTCGAAGGAGCAGGTGAAGGCGCTGGCGGTGGACGGCACGCGGATGATCAAGGAG
CAGGCGCAGGCGCGCCCGGGCACGCACTGGACCTATCAGTACTCGCCCGAAACCTTCAGCATGACCGAGCTGAGCTTCGC
GCGCGAAGTCTGCGACGCGGTTGCCCAGACCTGGCGCCCGACCCGCGACCACAAGATGATCGTCAACCTGCCGGCCACGG
TCGAGGCCTCGACACCGAACGTGTTTGCCGACCAGATCGAGTGGATGGACCGCAACCTCGGCTATCGCGACAGCATCGTG
CTGTCGGTGCATCCGCACAACGATCGCGGCACCGCGGTGGCTGCGACCGAGCTCGCGCTGCTGGCCGGCGCGGACCGCGT
CGAGGGCTGCCTGTTCGGCAACGGCGAGCGCACCGGCAACGTCGATCTCGTCACGCTCGCGCTCAATCTCTACACGCAGG
GCATCGATCCGGGCCTCGACTTCTCGGATATCGACGCGGTACGCGCCGTGGTCGAGCGCTGCAACCAGTTGCCGGTGCAT
CCGCGCCATCCGTATGCGGGCGATCTCGTCTTCACGGCGTTCTCGGGCTCGCATCAGGACGCGATCCGCAAGGGCCTCGC
GCGGCAGCGCGCCGACGCCCCCTGGGAAGTGCCCTATCTGCCGATCGATCCGGCCGATCTCGGCCGCGGCTACCAAGCGG
TGATCCGCGTCAACAGCCAGTCCGGCAAGGGCGGCGCTACCTACCTGCTCGAACGCGGCATGGGCTTCGCGCCGCCGCGC
CGCGTGCAGATCGAGTTCAGCCGTGCGGTGCAGGCGGTGGCCGACCGCTCCGGCGAGGAAGTGACGGGCGAGGCGATCTG
CGCGCTGTTCCGCCGCGAGTATCTGGAGACCACCGGGCCGGCCGCGCGCGTCGGCAGCACGATGACCTGGCAGCGGCGGG
AAATTGCGGCCGGCGGGACGCTCGCGAGCGACACCGTGGAGGCAATCGCCGTGCAGGCCGCGGCGGCGTTCTCCGCTGCG
GCCGGCACGACGGTCGAAGTGGCATCGATCGAGGCGCTGCGCGGCCGGGACGGCCGCTGCGCCGTGTTCGTCGGCGCGCG
GCTCGGCGGCGCCGCGCTGCGCTACGGGGTGGGGATCCACGAGGAACCGGCGGTGGCCGCCGTCGATGCGGTCATTAGCG
CGCTCAACCGTTCCGGCTGGCATTGCGCGGACCGTCGCGCCGCGGCCTGA

Upstream 100 bases:

>100_bases
CGCCGCATGCCGGCCGCGAAGCCGTGCGGCCCGTGCGCAGGCAGCCGCCATCCGGCTGCCGCCCCGTCTTCGCTCGCGAC
CGCATCGCCTCGAGGCCACC

Downstream 100 bases:

>100_bases
GGCTCGCGGGCCGGACGGCCGCCCGCCGCCCGCGGTGGGGCGCTTGGCGCCTCGGCTTGCGGCGCGGCCGCATGCACTGC
GCCACCGCCCCGTGAGATCC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 549; Mature: 549

Protein sequence:

>549_residues
MQRNPADKYRPFEPVRLNGRKWPSRTITQAPIWMSTDLRDGNQSLIEPMRIEQKLEFFEMLVAIGFKEIEVGFPSASQTD
FDFVRKLIDERRIPEDVTIEVLVQSREDLIARTFEALEGAPRAIVHLYNAIAPSFRQIVFGLSKEQVKALAVDGTRMIKE
QAQARPGTHWTYQYSPETFSMTELSFAREVCDAVAQTWRPTRDHKMIVNLPATVEASTPNVFADQIEWMDRNLGYRDSIV
LSVHPHNDRGTAVAATELALLAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGIDPGLDFSDIDAVRAVVERCNQLPVH
PRHPYAGDLVFTAFSGSHQDAIRKGLARQRADAPWEVPYLPIDPADLGRGYQAVIRVNSQSGKGGATYLLERGMGFAPPR
RVQIEFSRAVQAVADRSGEEVTGEAICALFRREYLETTGPAARVGSTMTWQRREIAAGGTLASDTVEAIAVQAAAAFSAA
AGTTVEVASIEALRGRDGRCAVFVGARLGGAALRYGVGIHEEPAVAAVDAVISALNRSGWHCADRRAAA

Sequences:

>Translated_549_residues
MQRNPADKYRPFEPVRLNGRKWPSRTITQAPIWMSTDLRDGNQSLIEPMRIEQKLEFFEMLVAIGFKEIEVGFPSASQTD
FDFVRKLIDERRIPEDVTIEVLVQSREDLIARTFEALEGAPRAIVHLYNAIAPSFRQIVFGLSKEQVKALAVDGTRMIKE
QAQARPGTHWTYQYSPETFSMTELSFAREVCDAVAQTWRPTRDHKMIVNLPATVEASTPNVFADQIEWMDRNLGYRDSIV
LSVHPHNDRGTAVAATELALLAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGIDPGLDFSDIDAVRAVVERCNQLPVH
PRHPYAGDLVFTAFSGSHQDAIRKGLARQRADAPWEVPYLPIDPADLGRGYQAVIRVNSQSGKGGATYLLERGMGFAPPR
RVQIEFSRAVQAVADRSGEEVTGEAICALFRREYLETTGPAARVGSTMTWQRREIAAGGTLASDTVEAIAVQAAAAFSAA
AGTTVEVASIEALRGRDGRCAVFVGARLGGAALRYGVGIHEEPAVAAVDAVISALNRSGWHCADRRAAA
>Mature_549_residues
MQRNPADKYRPFEPVRLNGRKWPSRTITQAPIWMSTDLRDGNQSLIEPMRIEQKLEFFEMLVAIGFKEIEVGFPSASQTD
FDFVRKLIDERRIPEDVTIEVLVQSREDLIARTFEALEGAPRAIVHLYNAIAPSFRQIVFGLSKEQVKALAVDGTRMIKE
QAQARPGTHWTYQYSPETFSMTELSFAREVCDAVAQTWRPTRDHKMIVNLPATVEASTPNVFADQIEWMDRNLGYRDSIV
LSVHPHNDRGTAVAATELALLAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGIDPGLDFSDIDAVRAVVERCNQLPVH
PRHPYAGDLVFTAFSGSHQDAIRKGLARQRADAPWEVPYLPIDPADLGRGYQAVIRVNSQSGKGGATYLLERGMGFAPPR
RVQIEFSRAVQAVADRSGEEVTGEAICALFRREYLETTGPAARVGSTMTWQRREIAAGGTLASDTVEAIAVQAAAAFSAA
AGTTVEVASIEALRGRDGRCAVFVGARLGGAALRYGVGIHEEPAVAAVDAVISALNRSGWHCADRRAAA

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=397, Percent_Identity=25.9445843828715, Blast_Score=99, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6324225, Length=570, Percent_Identity=47.719298245614, Blast_Score=533, Evalue=1e-152,
Organism=Saccharomyces cerevisiae, GI6324682, Length=442, Percent_Identity=54.7511312217195, Blast_Score=521, Evalue=1e-148,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 60190; Mature: 60190

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRNPADKYRPFEPVRLNGRKWPSRTITQAPIWMSTDLRDGNQSLIEPMRIEQKLEFFEM
CCCCCCCCCCCCCCEEECCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHH
LVAIGFKEIEVGFPSASQTDFDFVRKLIDERRIPEDVTIEVLVQSREDLIARTFEALEGA
HHHCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHCCC
PRAIVHLYNAIAPSFRQIVFGLSKEQVKALAVDGTRMIKEQAQARPGTHWTYQYSPETFS
HHHHHHHHHHHHHHHHHHHHCCCHHHHHEEECCHHHHHHHHHHCCCCCCEEEEECCCCCC
MTELSFAREVCDAVAQTWRPTRDHKMIVNLPATVEASTPNVFADQIEWMDRNLGYRDSIV
HHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEECCCCCHHHHHHHHHHCCCCCCCEEE
LSVHPHNDRGTAVAATELALLAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGIDPGLD
EEEECCCCCCCCHHHHHHHHHHCCCHHCEEEECCCCCCCCEEEEEEEEHHHHCCCCCCCC
FSDIDAVRAVVERCNQLPVHPRHPYAGDLVFTAFSGSHQDAIRKGLARQRADAPWEVPYL
HHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCEE
PIDPADLGRGYQAVIRVNSQSGKGGATYLLERGMGFAPPRRVQIEFSRAVQAVADRSGEE
CCCHHHHCCCEEEEEEEECCCCCCCHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHCCCCC
VTGEAICALFRREYLETTGPAARVGSTMTWQRREIAAGGTLASDTVEAIAVQAAAAFSAA
CHHHHHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHC
AGTTVEVASIEALRGRDGRCAVFVGARLGGAALRYGVGIHEEPAVAAVDAVISALNRSGW
CCCEEEEEEHHHHCCCCCCEEEEEECCCCCHHHHHCCCCCCCCHHHHHHHHHHHHCCCCC
HCADRRAAA
CCCCCCCCC
>Mature Secondary Structure
MQRNPADKYRPFEPVRLNGRKWPSRTITQAPIWMSTDLRDGNQSLIEPMRIEQKLEFFEM
CCCCCCCCCCCCCCEEECCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHH
LVAIGFKEIEVGFPSASQTDFDFVRKLIDERRIPEDVTIEVLVQSREDLIARTFEALEGA
HHHCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHCCC
PRAIVHLYNAIAPSFRQIVFGLSKEQVKALAVDGTRMIKEQAQARPGTHWTYQYSPETFS
HHHHHHHHHHHHHHHHHHHHCCCHHHHHEEECCHHHHHHHHHHCCCCCCEEEEECCCCCC
MTELSFAREVCDAVAQTWRPTRDHKMIVNLPATVEASTPNVFADQIEWMDRNLGYRDSIV
HHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEECCCCCHHHHHHHHHHCCCCCCCEEE
LSVHPHNDRGTAVAATELALLAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGIDPGLD
EEEECCCCCCCCHHHHHHHHHHCCCHHCEEEECCCCCCCCEEEEEEEEHHHHCCCCCCCC
FSDIDAVRAVVERCNQLPVHPRHPYAGDLVFTAFSGSHQDAIRKGLARQRADAPWEVPYL
HHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCEE
PIDPADLGRGYQAVIRVNSQSGKGGATYLLERGMGFAPPRRVQIEFSRAVQAVADRSGEE
CCCHHHHCCCEEEEEEEECCCCCCCHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHCCCCC
VTGEAICALFRREYLETTGPAARVGSTMTWQRREIAAGGTLASDTVEAIAVQAAAAFSAA
CHHHHHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHC
AGTTVEVASIEALRGRDGRCAVFVGARLGGAALRYGVGIHEEPAVAAVDAVISALNRSGW
CCCEEEEEEHHHHCCCCCCEEEEEECCCCCHHHHHCCCCCCCCHHHHHHHHHHHHCCCCC
HCADRRAAA
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA