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The map label for this gene is emrB [H]

Identifier: 238028637

GI number: 238028637

Start: 3525694

End: 3527244

Strand: Reverse

Name: emrB [H]

Synonym: bglu_1g31010

Alternate gene names: 238028637

Gene position: 3527244-3525694 (Counterclockwise)

Preceding gene: 238028638

Following gene: 238028634

Centisome position: 90.29

GC content: 66.92

Gene sequence:

>1551_bases
ATGGCTGCTTCGATGCCCCTGCGCCCGCTCTCGGGCGCCCAGCTCGCGCTCGGCACCCTGGCGGTGTCGCTCGCGATGTT
CATGAACGTGCTCGACTCGTCGATCGCGAACGTCGCGATTCCGACCATTTCAGGCGAACTCGGCGTGTCGGTCGACGAAG
GCACCTGGGTAATCACCATCTTCGCCGCCGCGAACGCGGTGTCGATCCCGCTGACCGGCTGGCTCACGCAGCGCTTCGGG
CAGGTGCGCCTGTTCGTCGGCTCGATCCTGCTGTTCGTGATCGCCTCCTGGCTGTGCGGGATGGCGCCGAACCTGCCGAC
GCTGCTCGGCGCACGGATCCTGCAGGGCGTGGTGGCCGGGCCGCTCGCGCCGCTCTCGCAGGCGCTGCTGCTCGGCGCCT
GGCCGAAGCATAAATCGTCGACGGCGCTCGCGCTGTGGTCGATGACGGCGCTGGTCGGACCGATCGCCGGGCCGTCGCTG
GGCGGCTGGATCACCTACGACTACAACTGGTCGTGGATCTTCTACATCAACGTGCCGGTGGGCATCTTCGCCGCGTTCGT
CACCTGGACCATCTTCCGCTCGCGCGAGTCGGCCACGCGGCGCCTGCCGATCGACACGGTCGGGCTGCTGCTGCTGATCG
TCTGGGTCGCGTCGCTGCAGGTGATGCTCGACAAGGGCAAGGATCTCGACTGGTTCAACTCGTCGTTCATCGTCGGGCTC
GCGGTGGTCGCGGCGATCGGCTTCGCGTTCTTCGTGGTGTGGGAGCTGACCGAGAAGAACCCGGTGGTGGACATCCGGCT
CTTCAAGGTGCGCAACTTCGCGGGCGGCACGATCGCGCTGTCGGTGGCCTATGGGATGTTCTTCGGCACGCTGGTGCTGC
TGCCGCAGTGGATGCAGGAGTATCTCGGCTATCGCGCGATCGATTCCGGTCTGACCACCGCGCCGCTCGGCGTGTTCGCG
GTGCTGCTCACGCCGCTGATGGGGCGCCTGCTGCCGCGCACCGATTCGCGCTATGTCGCCACGGTCGCGTTCGTCGGTTT
CGCGATCGTATTCTTCATGCGCACGCGCTTCTACACCGACGTGTCGAACTGGGACCTGGTGCTGCCGACCCTGCTGCAGG
GCATCCCGATGGCGATGTTCTTCGTGCCGCTGACCGCGATCATCCTGTCGGGGCTGCCGCCCGAGAAAATCCCGGCCGCC
GCGGGGCTGTCGAATTTCGGACGGACCTTCTGCGGCGCGGTCGGCACCTCGGTGATCGGCAACGCCTGGAACAACCGCAC
GGTGCTGCATCACGAGCGGCTGACCGAGCAGGCCTATGCGACCAATCCCGATTTCAGCCGGTCGATCGACGCGACGCAGT
CGATGCTGCACGTCGGCCGCGACACGGCGCACGCGCTGTTCAACCTGTCGGTGGACGGGCAGGCCGCCGTGATGGGCCTG
AACGACGTGTTCTACATCTCGGCCCTGATCTTCATCGCGATCATCCCGCTGATCTGGATTACCAAGCCGCACCGCTCGGC
CGCGCCGGTCGACGCGGGCGGGGCGCACTGA

Upstream 100 bases:

>100_bases
CGAGATCGCGCGGATCATCGCCGAGAACGCCGGCGGCCCGGTCCGGGCCGCCCAGCCGGCGCCTCGCGCGCCGCGGGCCT
GAGGCAAGCGGAACCGCTCC

Downstream 100 bases:

>100_bases
CGGTCCGGCGCGCCGACGGCGGCATGCCCGCGCGGCGGGCGGCGCTCAGAAGCGCTTGAGCAACTGGTGGTAGCGCATTC
CGAGTTCGAGCGCTGCCTGC

Product: EmrB/QacA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MAASMPLRPLSGAQLALGTLAVSLAMFMNVLDSSIANVAIPTISGELGVSVDEGTWVITIFAAANAVSIPLTGWLTQRFG
QVRLFVGSILLFVIASWLCGMAPNLPTLLGARILQGVVAGPLAPLSQALLLGAWPKHKSSTALALWSMTALVGPIAGPSL
GGWITYDYNWSWIFYINVPVGIFAAFVTWTIFRSRESATRRLPIDTVGLLLLIVWVASLQVMLDKGKDLDWFNSSFIVGL
AVVAAIGFAFFVVWELTEKNPVVDIRLFKVRNFAGGTIALSVAYGMFFGTLVLLPQWMQEYLGYRAIDSGLTTAPLGVFA
VLLTPLMGRLLPRTDSRYVATVAFVGFAIVFFMRTRFYTDVSNWDLVLPTLLQGIPMAMFFVPLTAIILSGLPPEKIPAA
AGLSNFGRTFCGAVGTSVIGNAWNNRTVLHHERLTEQAYATNPDFSRSIDATQSMLHVGRDTAHALFNLSVDGQAAVMGL
NDVFYISALIFIAIIPLIWITKPHRSAAPVDAGGAH

Sequences:

>Translated_516_residues
MAASMPLRPLSGAQLALGTLAVSLAMFMNVLDSSIANVAIPTISGELGVSVDEGTWVITIFAAANAVSIPLTGWLTQRFG
QVRLFVGSILLFVIASWLCGMAPNLPTLLGARILQGVVAGPLAPLSQALLLGAWPKHKSSTALALWSMTALVGPIAGPSL
GGWITYDYNWSWIFYINVPVGIFAAFVTWTIFRSRESATRRLPIDTVGLLLLIVWVASLQVMLDKGKDLDWFNSSFIVGL
AVVAAIGFAFFVVWELTEKNPVVDIRLFKVRNFAGGTIALSVAYGMFFGTLVLLPQWMQEYLGYRAIDSGLTTAPLGVFA
VLLTPLMGRLLPRTDSRYVATVAFVGFAIVFFMRTRFYTDVSNWDLVLPTLLQGIPMAMFFVPLTAIILSGLPPEKIPAA
AGLSNFGRTFCGAVGTSVIGNAWNNRTVLHHERLTEQAYATNPDFSRSIDATQSMLHVGRDTAHALFNLSVDGQAAVMGL
NDVFYISALIFIAIIPLIWITKPHRSAAPVDAGGAH
>Mature_515_residues
AASMPLRPLSGAQLALGTLAVSLAMFMNVLDSSIANVAIPTISGELGVSVDEGTWVITIFAAANAVSIPLTGWLTQRFGQ
VRLFVGSILLFVIASWLCGMAPNLPTLLGARILQGVVAGPLAPLSQALLLGAWPKHKSSTALALWSMTALVGPIAGPSLG
GWITYDYNWSWIFYINVPVGIFAAFVTWTIFRSRESATRRLPIDTVGLLLLIVWVASLQVMLDKGKDLDWFNSSFIVGLA
VVAAIGFAFFVVWELTEKNPVVDIRLFKVRNFAGGTIALSVAYGMFFGTLVLLPQWMQEYLGYRAIDSGLTTAPLGVFAV
LLTPLMGRLLPRTDSRYVATVAFVGFAIVFFMRTRFYTDVSNWDLVLPTLLQGIPMAMFFVPLTAIILSGLPPEKIPAAA
GLSNFGRTFCGAVGTSVIGNAWNNRTVLHHERLTEQAYATNPDFSRSIDATQSMLHVGRDTAHALFNLSVDGQAAVMGLN
DVFYISALIFIAIIPLIWITKPHRSAAPVDAGGAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=499, Percent_Identity=48.6973947895792, Blast_Score=474, Evalue=1e-135,
Organism=Escherichia coli, GI1788710, Length=505, Percent_Identity=44.7524752475248, Blast_Score=420, Evalue=1e-119,
Organism=Escherichia coli, GI1790195, Length=380, Percent_Identity=29.2105263157895, Blast_Score=149, Evalue=6e-37,
Organism=Escherichia coli, GI87081983, Length=410, Percent_Identity=24.390243902439, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1788392, Length=409, Percent_Identity=28.8508557457213, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55683; Mature: 55552

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAASMPLRPLSGAQLALGTLAVSLAMFMNVLDSSIANVAIPTISGELGVSVDEGTWVITI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEECCCCEEEEE
FAAANAVSIPLTGWLTQRFGQVRLFVGSILLFVIASWLCGMAPNLPTLLGARILQGVVAG
EECCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHC
PLAPLSQALLLGAWPKHKSSTALALWSMTALVGPIAGPSLGGWITYDYNWSWIFYINVPV
CHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCEEEEEEEECCH
GIFAAFVTWTIFRSRESATRRLPIDTVGLLLLIVWVASLQVMLDKGKDLDWFNSSFIVGL
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
AVVAAIGFAFFVVWELTEKNPVVDIRLFKVRNFAGGTIALSVAYGMFFGTLVLLPQWMQE
HHHHHHHHHHHHHHEECCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
YLGYRAIDSGLTTAPLGVFAVLLTPLMGRLLPRTDSRYVATVAFVGFAIVFFMRTRFYTD
HCCCCHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
VSNWDLVLPTLLQGIPMAMFFVPLTAIILSGLPPEKIPAAAGLSNFGRTFCGAVGTSVIG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHHCCHHHHHHHHHHHHHHHHHC
NAWNNRTVLHHERLTEQAYATNPDFSRSIDATQSMLHVGRDTAHALFNLSVDGQAAVMGL
CCCCCCEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHEEEECCCCCEEEEEC
NDVFYISALIFIAIIPLIWITKPHRSAAPVDAGGAH
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AASMPLRPLSGAQLALGTLAVSLAMFMNVLDSSIANVAIPTISGELGVSVDEGTWVITI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEECCCCEEEEE
FAAANAVSIPLTGWLTQRFGQVRLFVGSILLFVIASWLCGMAPNLPTLLGARILQGVVAG
EECCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHC
PLAPLSQALLLGAWPKHKSSTALALWSMTALVGPIAGPSLGGWITYDYNWSWIFYINVPV
CHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCEEEEEEEECCH
GIFAAFVTWTIFRSRESATRRLPIDTVGLLLLIVWVASLQVMLDKGKDLDWFNSSFIVGL
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
AVVAAIGFAFFVVWELTEKNPVVDIRLFKVRNFAGGTIALSVAYGMFFGTLVLLPQWMQE
HHHHHHHHHHHHHHEECCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
YLGYRAIDSGLTTAPLGVFAVLLTPLMGRLLPRTDSRYVATVAFVGFAIVFFMRTRFYTD
HCCCCHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
VSNWDLVLPTLLQGIPMAMFFVPLTAIILSGLPPEKIPAAAGLSNFGRTFCGAVGTSVIG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHHCCHHHHHHHHHHHHHHHHHC
NAWNNRTVLHHERLTEQAYATNPDFSRSIDATQSMLHVGRDTAHALFNLSVDGQAAVMGL
CCCCCCEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHEEEECCCCCEEEEEC
NDVFYISALIFIAIIPLIWITKPHRSAAPVDAGGAH
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]