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The map label for this gene is nudB [H]

Identifier: 238028611

GI number: 238028611

Start: 3496394

End: 3496870

Strand: Reverse

Name: nudB [H]

Synonym: bglu_1g30750

Alternate gene names: 238028611

Gene position: 3496870-3496394 (Counterclockwise)

Preceding gene: 238028612

Following gene: 238028610

Centisome position: 89.51

GC content: 69.39

Gene sequence:

>477_bases
ATGACGAAGCCGCCGAAAATCCCCGAATCCGTCCTGGTCGTGATCCATACCGCCGCACTTGACGTGCTGATCATCAAGCG
CGCCGACCTGCCCGGCTTCTGGCAGTCGGTGACGGGCTCGAAGGACCGGCCGGACGAGCCGCTCGCCGCCACCGCGATTC
GCGAGGTCGGCGAGGAGACCGGCATCGTGGTCGGCAGCGAGGGCGTGCCGCTCGCCGCGCTCGTCGACTGGCGCCACCAG
ATCGAGTACTCGATCTATCCGCAATACCTGCACCGCTACGCGCCCGGCGTCACGCGCAACACCGAGCACTGGTTCAGCCT
CGAGGTGCCCGCCGGGATTGGCGTCACGCTGTCGCCGCGCGAGCACACCGAGCATCTCTGGCTGCCGTACCGCGAGGCGG
CCGCGCGCTGCTACTCGCCGTCCAACGCCGAGGCGATCCTTCAGCTTCCCGCGCGCCTCGCGGCGCGTGAGGCATGA

Upstream 100 bases:

>100_bases
TGCCCCGACACGCGGCGGCCTTCGTTTTGCGGTACAGTCTGCCGGTCCCGAAGTCCCCGGCCGGCCGGCGCGAAGTTCCG
CGCCGGCGGCCGCACCCGCA

Downstream 100 bases:

>100_bases
GCGGCGGCAAGCGGCTGCGCCTCACCCAGTTGCGGGACCTGTTCCTGCAGGAGCGCGGGTCGTCGTCGCGGCTCGCGTTC
AGCGCCGGCAACACCGTCAC

Product: dihydroneopterin triphosphate pyrophosphatase

Products: dAMP; pyrophosphate [C]

Alternate protein names: dATP pyrophosphohydrolase [H]

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MTKPPKIPESVLVVIHTAALDVLIIKRADLPGFWQSVTGSKDRPDEPLAATAIREVGEETGIVVGSEGVPLAALVDWRHQ
IEYSIYPQYLHRYAPGVTRNTEHWFSLEVPAGIGVTLSPREHTEHLWLPYREAAARCYSPSNAEAILQLPARLAAREA

Sequences:

>Translated_158_residues
MTKPPKIPESVLVVIHTAALDVLIIKRADLPGFWQSVTGSKDRPDEPLAATAIREVGEETGIVVGSEGVPLAALVDWRHQ
IEYSIYPQYLHRYAPGVTRNTEHWFSLEVPAGIGVTLSPREHTEHLWLPYREAAARCYSPSNAEAILQLPARLAAREA
>Mature_157_residues
TKPPKIPESVLVVIHTAALDVLIIKRADLPGFWQSVTGSKDRPDEPLAATAIREVGEETGIVVGSEGVPLAALVDWRHQI
EYSIYPQYLHRYAPGVTRNTEHWFSLEVPAGIGVTLSPREHTEHLWLPYREAAARCYSPSNAEAILQLPARLAAREA

Specific function: Catalyzes the hydrolysis of dihydroneopterin triphosphate to dihydroneopterin monophosphate and pyrophosphate. Required for efficient folate biosynthesis. Can also hydrolyze nucleoside triphosphates with a preference for dATP [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1788172, Length=148, Percent_Identity=43.2432432432432, Blast_Score=99, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003564
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 17483; Mature: 17352

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKPPKIPESVLVVIHTAALDVLIIKRADLPGFWQSVTGSKDRPDEPLAATAIREVGEET
CCCCCCCCHHHHHHHHHHHHEEEEEEECCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHC
GIVVGSEGVPLAALVDWRHQIEYSIYPQYLHRYAPGVTRNTEHWFSLEVPAGIGVTLSPR
CEEECCCCCCHHHHHHHHHHCCEEECHHHHHHHCCCCCCCCCCEEEEECCCCCCEEECCC
EHTEHLWLPYREAAARCYSPSNAEAILQLPARLAAREA
CCCCEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKPPKIPESVLVVIHTAALDVLIIKRADLPGFWQSVTGSKDRPDEPLAATAIREVGEET
CCCCCCCHHHHHHHHHHHHEEEEEEECCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHC
GIVVGSEGVPLAALVDWRHQIEYSIYPQYLHRYAPGVTRNTEHWFSLEVPAGIGVTLSPR
CEEECCCCCCHHHHHHHHHHCCEEECHHHHHHHCCCCCCCCCCEEEEECCCCCCEEECCC
EHTEHLWLPYREAAARCYSPSNAEAILQLPARLAAREA
CCCCEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxy-ATP; H2O [C]

Specific reaction: deoxy-ATP + H2O = dAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]