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The map label for this gene is gloA [H]

Identifier: 238028594

GI number: 238028594

Start: 3475177

End: 3475566

Strand: Reverse

Name: gloA [H]

Synonym: bglu_1g30580

Alternate gene names: 238028594

Gene position: 3475566-3475177 (Counterclockwise)

Preceding gene: 238028595

Following gene: 238028588

Centisome position: 88.97

GC content: 63.59

Gene sequence:

>390_bases
ATGCGAATGCTGCATACGATGCTGCGCGTCGGCGATCTCGACCGCTCGATCCAGTTCTACACCGGGCTGCTCGGCATGAA
GCTGCTGCGCCGCAACGACTATCCGGAAGGCAGGTTCACGCTCGCGTTCGTCGGTTACGAAGCGGAATCGACCGGCACCG
TGATCGAGCTGACCCACAACTGGGACACGCCGTCCTATGAGATCGGCACCGGTTTCGGCCACCTCGCGATCGAGGTCGAC
GACGCCTACGCGGCCTGCGAGCGCATCAAGGCGCAGGGCGGCAAGGTCACGCGCGAAGCGGGCCCGATGAAGCACGGCAC
GACCGTGATCGCATTCGTCGAGGACCCGGACGGCTACAAGATCGAATTCATCCAGCGCAAGTCGCACTGA

Upstream 100 bases:

>100_bases
GGCCGGGCCGGTTCGCGGATGCGCCGGCCCGCCGGCGAAAATCGGCCGCGTCTGGTAAATTCGGCTGTTCCCCCAGATGG
TTTACACGGAGTTCCCCACC

Downstream 100 bases:

>100_bases
GCCGGATGCCCATGAAAAACGGCGCCTCACGCGAGGCGCCGTTTTTCATGGCGCGGCATGCTGCGCGCCTACAGCGCGGG
CAGCAGCTCGGGCGGATGGT

Product: Lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 129; Mature: 129

Protein sequence:

>129_residues
MRMLHTMLRVGDLDRSIQFYTGLLGMKLLRRNDYPEGRFTLAFVGYEAESTGTVIELTHNWDTPSYEIGTGFGHLAIEVD
DAYAACERIKAQGGKVTREAGPMKHGTTVIAFVEDPDGYKIEFIQRKSH

Sequences:

>Translated_129_residues
MRMLHTMLRVGDLDRSIQFYTGLLGMKLLRRNDYPEGRFTLAFVGYEAESTGTVIELTHNWDTPSYEIGTGFGHLAIEVD
DAYAACERIKAQGGKVTREAGPMKHGTTVIAFVEDPDGYKIEFIQRKSH
>Mature_129_residues
MRMLHTMLRVGDLDRSIQFYTGLLGMKLLRRNDYPEGRFTLAFVGYEAESTGTVIELTHNWDTPSYEIGTGFGHLAIEVD
DAYAACERIKAQGGKVTREAGPMKHGTTVIAFVEDPDGYKIEFIQRKSH

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=149, Percent_Identity=38.9261744966443, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1787940, Length=127, Percent_Identity=66.1417322834646, Blast_Score=180, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6323639, Length=139, Percent_Identity=38.8489208633094, Blast_Score=97, Evalue=6e-22,
Organism=Drosophila melanogaster, GI20129743, Length=150, Percent_Identity=40.6666666666667, Blast_Score=98, Evalue=1e-21,
Organism=Drosophila melanogaster, GI19920404, Length=138, Percent_Identity=33.3333333333333, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 14529; Mature: 14529

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMLHTMLRVGDLDRSIQFYTGLLGMKLLRRNDYPEGRFTLAFVGYEAESTGTVIELTHN
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCEEEEEEEC
WDTPSYEIGTGFGHLAIEVDDAYAACERIKAQGGKVTREAGPMKHGTTVIAFVEDPDGYK
CCCCCEEECCCCEEEEEEECHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEECCCCCE
IEFIQRKSH
EEEEEECCC
>Mature Secondary Structure
MRMLHTMLRVGDLDRSIQFYTGLLGMKLLRRNDYPEGRFTLAFVGYEAESTGTVIELTHN
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCEEEEEEEC
WDTPSYEIGTGFGHLAIEVDDAYAACERIKAQGGKVTREAGPMKHGTTVIAFVEDPDGYK
CCCCCEEECCCCEEEEEEECHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEECCCCCE
IEFIQRKSH
EEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]