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The map label for this gene is emrB [H]

Identifier: 238028562

GI number: 238028562

Start: 3437920

End: 3439524

Strand: Direct

Name: emrB [H]

Synonym: bglu_1g30260

Alternate gene names: 238028562

Gene position: 3437920-3439524 (Clockwise)

Preceding gene: 238028561

Following gene: 238028563

Centisome position: 88.0

GC content: 68.97

Gene sequence:

>1605_bases
ATGGCTGAGTCCGCCACGCCGGCCGGCGCACCGGCCGCCCCCGCCCCGGCCGCCGAGCGCGCCACCGCCTCCGACTGGAT
CGCGGTGGCAGCCGGCTCGCTCGGCGCGCTGATGGCCACGCTCGACATCTCGATCACCAACTCGGCGCTGCCGCAGATCC
AGGGCGAGATCGGCGCGACCGGCACCGAGGGCACCTGGATCTCCACCGGCTACCTGATGTCGGAAATCGTGATGATCCCG
CTCGCCGCATGGCTCACGCGCGTGTTCGGGCTGAAGAACTTCCTGCTCGCCTCGTCGGTGCTGTTCATCGCGTTCTCGAT
GATCTGCGGCTGGTCGAACACGCTCGCGCTGATGGTGGTCGGCCGGATCGGCCAGGGCTTCACGGGCGGCGCGATGATCC
CGACCGCGCAGACCATCATCCGCACCCGGCTGCCGCCCTCGCAGCTGCCGGTGGGCATGACGCTGTTCGGCCTGATCGTG
CTGCTCGGGCCGCTGTTCGGGCCGGTGCTGGGCGGCTGGCTGGCCGAGAACGTCAGCTGGAGCTGGTGCTTCTTCATCAA
CCTGCCGGTGTGCCTGCTGCTGATCACGCTGCTGGTGGTGGGCCTCAGCTCCGACAAGCCGCAGTGGCGGCAGTTCTTCG
ACGCCGACTGGGTCGGCATCGCGGGCCTCGCGATCGGGCTGTCGTCGCTGACGGTGGTGCTCGAGGAAGGCCAGCGCGAG
CGCTGGTTCGAGTCGCAGATGATCGTCACGCTGAGCTGGGTCTCGTTCGCCGGCATGCTGCTGATCGCGATCTCGCAGCT
CACGGCCAGGCGGCCGATCCTGCGGCTCTCGCTGATGCGCAACCCGCGCTACGCGAGCGTGATCCTGATCGTCGCGGCGG
TCGGTGCCGGCCTGTACGGCGTGTCGTACCTGCTGCCGCAGTTCCTCGCGATCGTGTCCGGCTACAACGCCGAGCAGGCG
GGCGAGATCATGCTGCTGTCGGGCCTGCCCGCGTTCCTGATGATGCCGATCCTGCCGAGGCTGCTCGGCAAGGTCGATTT
CCGGATCCTCGTGATCACCGGGCTGCTGCTGTTCTTCCTGAGCTGCATGCTCGACATCTCGCTGACCGCGCAGAGCGTCG
GCCACGACTTCGTCTGGTCGCAGCTGATCCGCGGCGTGGCCCAGATGCTCGCGATGATGCCGCTGAACCAGGCCTCGATG
GCCGCCGTCTCGCGCGAGGAATCGGGCGACGCGGCCGGGCTCTACAACATGGCGCGCAATCTCGGCGGCTCGATCGGGCT
GGCCATCATCGGCACCATCATCGACCGGCGCACCACGTTCCATACCGCCGCGCTGCGCGAGTCGCTCAGCGCCAACTCGC
CGGTCGGGCAGGAGCGCATCGCCAGCTATGCGGCGAGCTGGTTCGCGCGCACCGGCGACATGGCCTACTCGAAGCTGCGC
GCGCTCGGCCAGATCGCGCAGCAGATCCAGGTGCAGGCCACCGTGATGACCTACTCCGAAACCTTCTACCTGCTCGGACT
CGCGCTGATCGCCTGCGTCCCGCTCGCGCTGCTGCTCAGAACGCCGCGCCGCGGTTCGCCGCCGCCCGCCTCGTCCGGCC
ATTGA

Upstream 100 bases:

>100_bases
GTGCGGATCCACGTCGACACCGACGACCAGGCGCGCCGCGTGCTGCTGCCGGGCCTGTCGGTGACCGTCGACGTCGATAC
GCGCGACGACGGGAACCAGC

Downstream 100 bases:

>100_bases
ATCGATGCAGATCATGACGTTTTCCCCGCCCGCCCGCCGCCGCGCCGCCGCGCCCCTCACGCTGCTCGCGCTCGCCGGCC
TGCTCGCCGGCTGCACGGTC

Product: EmrB/QacA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 534; Mature: 533

Protein sequence:

>534_residues
MAESATPAGAPAAPAPAAERATASDWIAVAAGSLGALMATLDISITNSALPQIQGEIGATGTEGTWISTGYLMSEIVMIP
LAAWLTRVFGLKNFLLASSVLFIAFSMICGWSNTLALMVVGRIGQGFTGGAMIPTAQTIIRTRLPPSQLPVGMTLFGLIV
LLGPLFGPVLGGWLAENVSWSWCFFINLPVCLLLITLLVVGLSSDKPQWRQFFDADWVGIAGLAIGLSSLTVVLEEGQRE
RWFESQMIVTLSWVSFAGMLLIAISQLTARRPILRLSLMRNPRYASVILIVAAVGAGLYGVSYLLPQFLAIVSGYNAEQA
GEIMLLSGLPAFLMMPILPRLLGKVDFRILVITGLLLFFLSCMLDISLTAQSVGHDFVWSQLIRGVAQMLAMMPLNQASM
AAVSREESGDAAGLYNMARNLGGSIGLAIIGTIIDRRTTFHTAALRESLSANSPVGQERIASYAASWFARTGDMAYSKLR
ALGQIAQQIQVQATVMTYSETFYLLGLALIACVPLALLLRTPRRGSPPPASSGH

Sequences:

>Translated_534_residues
MAESATPAGAPAAPAPAAERATASDWIAVAAGSLGALMATLDISITNSALPQIQGEIGATGTEGTWISTGYLMSEIVMIP
LAAWLTRVFGLKNFLLASSVLFIAFSMICGWSNTLALMVVGRIGQGFTGGAMIPTAQTIIRTRLPPSQLPVGMTLFGLIV
LLGPLFGPVLGGWLAENVSWSWCFFINLPVCLLLITLLVVGLSSDKPQWRQFFDADWVGIAGLAIGLSSLTVVLEEGQRE
RWFESQMIVTLSWVSFAGMLLIAISQLTARRPILRLSLMRNPRYASVILIVAAVGAGLYGVSYLLPQFLAIVSGYNAEQA
GEIMLLSGLPAFLMMPILPRLLGKVDFRILVITGLLLFFLSCMLDISLTAQSVGHDFVWSQLIRGVAQMLAMMPLNQASM
AAVSREESGDAAGLYNMARNLGGSIGLAIIGTIIDRRTTFHTAALRESLSANSPVGQERIASYAASWFARTGDMAYSKLR
ALGQIAQQIQVQATVMTYSETFYLLGLALIACVPLALLLRTPRRGSPPPASSGH
>Mature_533_residues
AESATPAGAPAAPAPAAERATASDWIAVAAGSLGALMATLDISITNSALPQIQGEIGATGTEGTWISTGYLMSEIVMIPL
AAWLTRVFGLKNFLLASSVLFIAFSMICGWSNTLALMVVGRIGQGFTGGAMIPTAQTIIRTRLPPSQLPVGMTLFGLIVL
LGPLFGPVLGGWLAENVSWSWCFFINLPVCLLLITLLVVGLSSDKPQWRQFFDADWVGIAGLAIGLSSLTVVLEEGQRER
WFESQMIVTLSWVSFAGMLLIAISQLTARRPILRLSLMRNPRYASVILIVAAVGAGLYGVSYLLPQFLAIVSGYNAEQAG
EIMLLSGLPAFLMMPILPRLLGKVDFRILVITGLLLFFLSCMLDISLTAQSVGHDFVWSQLIRGVAQMLAMMPLNQASMA
AVSREESGDAAGLYNMARNLGGSIGLAIIGTIIDRRTTFHTAALRESLSANSPVGQERIASYAASWFARTGDMAYSKLRA
LGQIAQQIQVQATVMTYSETFYLLGLALIACVPLALLLRTPRRGSPPPASSGH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=483, Percent_Identity=31.2629399585921, Blast_Score=237, Evalue=2e-63,
Organism=Escherichia coli, GI1788710, Length=517, Percent_Identity=28.4332688588008, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI87081983, Length=412, Percent_Identity=25.9708737864078, Blast_Score=112, Evalue=8e-26,
Organism=Escherichia coli, GI1790195, Length=423, Percent_Identity=26.7139479905437, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1788392, Length=410, Percent_Identity=27.5609756097561, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6325455, Length=191, Percent_Identity=28.2722513089005, Blast_Score=74, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6322958, Length=191, Percent_Identity=23.5602094240838, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6321663, Length=149, Percent_Identity=25.503355704698, Blast_Score=64, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6319770, Length=272, Percent_Identity=24.6323529411765, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 57001; Mature: 56870

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAESATPAGAPAAPAPAAERATASDWIAVAAGSLGALMATLDISITNSALPQIQGEIGAT
CCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHCHHHHHHHHHEEEECCHHHHHHCCCCCCC
GTEGTWISTGYLMSEIVMIPLAAWLTRVFGLKNFLLASSVLFIAFSMICGWSNTLALMVV
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
GRIGQGFTGGAMIPTAQTIIRTRLPPSQLPVGMTLFGLIVLLGPLFGPVLGGWLAENVSW
HHCCCCCCCCCCCHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SWCFFINLPVCLLLITLLVVGLSSDKPQWRQFFDADWVGIAGLAIGLSSLTVVLEEGQRE
EEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHH
RWFESQMIVTLSWVSFAGMLLIAISQLTARRPILRLSLMRNPRYASVILIVAAVGAGLYG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
VSYLLPQFLAIVSGYNAEQAGEIMLLSGLPAFLMMPILPRLLGKVDFRILVITGLLLFFL
HHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
SCMLDISLTAQSVGHDFVWSQLIRGVAQMLAMMPLNQASMAAVSREESGDAAGLYNMARN
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHH
LGGSIGLAIIGTIIDRRTTFHTAALRESLSANSPVGQERIASYAASWFARTGDMAYSKLR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHH
ALGQIAQQIQVQATVMTYSETFYLLGLALIACVPLALLLRTPRRGSPPPASSGH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AESATPAGAPAAPAPAAERATASDWIAVAAGSLGALMATLDISITNSALPQIQGEIGAT
CCCCCCCCCCCCCCCCHHCCCHHHHHHHHHCHHHHHHHHHEEEECCHHHHHHCCCCCCC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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]