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The map label for this gene is oprM [H]

Identifier: 238028563

GI number: 238028563

Start: 3439529

End: 3441079

Strand: Direct

Name: oprM [H]

Synonym: bglu_1g30270

Alternate gene names: 238028563

Gene position: 3439529-3441079 (Clockwise)

Preceding gene: 238028562

Following gene: 238028565

Centisome position: 88.05

GC content: 74.98

Gene sequence:

>1551_bases
ATGCAGATCATGACGTTTTCCCCGCCCGCCCGCCGCCGCGCCGCCGCGCCCCTCACGCTGCTCGCGCTCGCCGGCCTGCT
CGCCGGCTGCACGGTCGGCCCGGACTATCACGGCGCGCCGCCCGCGCCCGCCGACGCCGGCTTCGTGCGCGCGCCGCAGG
CCGGCGTGCTGGCCGCCGCGCCGGCGCCGAGCGCCTGGTGGCACGCGCTCGGCGACCCGCAGCTGGACGCGCTGATCGCC
GCCGCGCTGGCCCATAACCCGGACCTGCACGCGGCGCAGGCGCGCCTGCGCGAGGCGCGCGCGCAACTGAGCCGGCAGCG
CGCCGACGCGCTGCCGAAGCTCGGCGCCGACGCAGCCGCGCTGCGCACGCGCGAGCCCGACGCGAACGCGTTCGCGGCGC
TGACCGGCTCGAGCGGCGCCAGCGGCTCGGGCAACGCTGCGTCGCGGCCCGGCACCGGCCCGATCCAGCTCTACACGGCC
AGCTTCGACGCGAGCTGGGAAATCGACCTGTTCGGCGGCACGCGGCGCGCGATCGAGGCCGCCTCCGCGCAGGCCGAGGC
CGTCGACGCCGATCTCGCCGACACCCAGGTCTCGCTCGCGGCCGAAGTCGCGAACGCCTACGTCGGCCTGCGCGACCAGC
AGCAGCGCCTCGCGCTGTCGCGGCGCACCGCCGAACTGCAGCAGCGCATGCTCGAGCTGACGTTGCAGCGGCGCGATCGC
GGCGTGGTGGACGATGCCGACATCGAGCGGCTCACCACCCAGGTGGAAAACACGCGCGCCACGCTGATCCCGCTCGACGC
GCAGGTGACCGAGTCGCTCGACCGCCTCGCGATCCTCACGGGACGCGCGCCGGGCACGCTCGATGCGCAGCTCTCGGCAC
CCGGCGCCGCGCTGCCGACGCTGCCCGCGACGGTGGCGATCGGCGACGCCGGCGCGCTCCTCAAGCAGCGTCCCGACATC
CGTGCGGCCGAGCGCCGGCTCGCCTCCAGCAACGCGCAGATCGGCGAGCGCGTGGCCGACTACTTTCCGAAGGTCACGCT
GTTCGGCGACCTCGGCTTCAGCGCGTCCGACCCGGGCCACCTGTTGCGCAAGCAGAACTACTCGTGGATCGGCGCGCCCT
ACCTGCAGTGGAACCTGTTCGACTTCGGCCGCACGCGCGGTGCCGTGCGCGCGGCCGAAGCCGCGCGCGACGAGGCCGAG
GCGAACTACACGAAGGCCGTGCTGGCCGCGCTGCAGGACGCCAACACCGCCCTGTCGCGTTACGGGCACCAGCGCGACCA
CGTCGTCGCGCTGGCCAAGATCGAGACCTCGGCCACGCGCTCGGCCGCGCTGATGAACCAGCGCTACCGGGCCGGCGTGT
CGTCGCTGATCGACCTGCTCGACACCCAGCGCGCGGCGCTCTCGGCACGGCAGAACGTGATCGCCGGACAGGCCGAGCTG
CTCGACGACTACATCTCGCTGCAAAAGAGCCTCGGCCTCGGCTGGCAGACCGACGCGACCGCGCCGCAGGTGCCGGCCGA
CGCGGCGGCCGGCGCGAAGCCGGCCGGCTGA

Upstream 100 bases:

>100_bases
CTGCTCGGACTCGCGCTGATCGCCTGCGTCCCGCTCGCGCTGCTGCTCAGAACGCCGCGCCGCGGTTCGCCGCCGCCCGC
CTCGTCCGGCCATTGAATCG

Downstream 100 bases:

>100_bases
GGCCGGCGGCTGCGGCGCGACCGCGGCGCCGACGGCGGCCTCAGCGCTCGATGCGCGCGCGCGTGGTGCCGAGCAGCGCC
GCCATCGCGCGGCGCGCCGC

Product: RND efflux system outer membrane lipoprotein NodT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MQIMTFSPPARRRAAAPLTLLALAGLLAGCTVGPDYHGAPPAPADAGFVRAPQAGVLAAAPAPSAWWHALGDPQLDALIA
AALAHNPDLHAAQARLREARAQLSRQRADALPKLGADAAALRTREPDANAFAALTGSSGASGSGNAASRPGTGPIQLYTA
SFDASWEIDLFGGTRRAIEAASAQAEAVDADLADTQVSLAAEVANAYVGLRDQQQRLALSRRTAELQQRMLELTLQRRDR
GVVDDADIERLTTQVENTRATLIPLDAQVTESLDRLAILTGRAPGTLDAQLSAPGAALPTLPATVAIGDAGALLKQRPDI
RAAERRLASSNAQIGERVADYFPKVTLFGDLGFSASDPGHLLRKQNYSWIGAPYLQWNLFDFGRTRGAVRAAEAARDEAE
ANYTKAVLAALQDANTALSRYGHQRDHVVALAKIETSATRSAALMNQRYRAGVSSLIDLLDTQRAALSARQNVIAGQAEL
LDDYISLQKSLGLGWQTDATAPQVPADAAAGAKPAG

Sequences:

>Translated_516_residues
MQIMTFSPPARRRAAAPLTLLALAGLLAGCTVGPDYHGAPPAPADAGFVRAPQAGVLAAAPAPSAWWHALGDPQLDALIA
AALAHNPDLHAAQARLREARAQLSRQRADALPKLGADAAALRTREPDANAFAALTGSSGASGSGNAASRPGTGPIQLYTA
SFDASWEIDLFGGTRRAIEAASAQAEAVDADLADTQVSLAAEVANAYVGLRDQQQRLALSRRTAELQQRMLELTLQRRDR
GVVDDADIERLTTQVENTRATLIPLDAQVTESLDRLAILTGRAPGTLDAQLSAPGAALPTLPATVAIGDAGALLKQRPDI
RAAERRLASSNAQIGERVADYFPKVTLFGDLGFSASDPGHLLRKQNYSWIGAPYLQWNLFDFGRTRGAVRAAEAARDEAE
ANYTKAVLAALQDANTALSRYGHQRDHVVALAKIETSATRSAALMNQRYRAGVSSLIDLLDTQRAALSARQNVIAGQAEL
LDDYISLQKSLGLGWQTDATAPQVPADAAAGAKPAG
>Mature_516_residues
MQIMTFSPPARRRAAAPLTLLALAGLLAGCTVGPDYHGAPPAPADAGFVRAPQAGVLAAAPAPSAWWHALGDPQLDALIA
AALAHNPDLHAAQARLREARAQLSRQRADALPKLGADAAALRTREPDANAFAALTGSSGASGSGNAASRPGTGPIQLYTA
SFDASWEIDLFGGTRRAIEAASAQAEAVDADLADTQVSLAAEVANAYVGLRDQQQRLALSRRTAELQQRMLELTLQRRDR
GVVDDADIERLTTQVENTRATLIPLDAQVTESLDRLAILTGRAPGTLDAQLSAPGAALPTLPATVAIGDAGALLKQRPDI
RAAERRLASSNAQIGERVADYFPKVTLFGDLGFSASDPGHLLRKQNYSWIGAPYLQWNLFDFGRTRGAVRAAEAARDEAE
ANYTKAVLAALQDANTALSRYGHQRDHVVALAKIETSATRSAALMNQRYRAGVSSLIDLLDTQRAALSARQNVIAGQAEL
LDDYISLQKSLGLGWQTDATAPQVPADAAAGAKPAG

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=488, Percent_Identity=25.8196721311475, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI2367348, Length=447, Percent_Identity=26.6219239373602, Blast_Score=92, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 54233; Mature: 54233

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIMTFSPPARRRAAAPLTLLALAGLLAGCTVGPDYHGAPPAPADAGFVRAPQAGVLAAA
CEEEECCCCHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCCCCEEEEC
PAPSAWWHALGDPQLDALIAAALAHNPDLHAAQARLREARAQLSRQRADALPKLGADAAA
CCCHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
LRTREPDANAFAALTGSSGASGSGNAASRPGTGPIQLYTASFDASWEIDLFGGTRRAIEA
HCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCHHHHHH
ASAQAEAVDADLADTQVSLAAEVANAYVGLRDQQQRLALSRRTAELQQRMLELTLQRRDR
HHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GVVDDADIERLTTQVENTRATLIPLDAQVTESLDRLAILTGRAPGTLDAQLSAPGAALPT
CCCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCC
LPATVAIGDAGALLKQRPDIRAAERRLASSNAQIGERVADYFPKVTLFGDLGFSASDPGH
CCCEEEECCCHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHCCCEEEEECCCCCCCCCHH
LLRKQNYSWIGAPYLQWNLFDFGRTRGAVRAAEAARDEAEANYTKAVLAALQDANTALSR
HHHHCCCCEECCCEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGHQRDHVVALAKIETSATRSAALMNQRYRAGVSSLIDLLDTQRAALSARQNVIAGQAEL
CCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDDYISLQKSLGLGWQTDATAPQVPADAAAGAKPAG
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQIMTFSPPARRRAAAPLTLLALAGLLAGCTVGPDYHGAPPAPADAGFVRAPQAGVLAAA
CEEEECCCCHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCCCCEEEEC
PAPSAWWHALGDPQLDALIAAALAHNPDLHAAQARLREARAQLSRQRADALPKLGADAAA
CCCHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
LRTREPDANAFAALTGSSGASGSGNAASRPGTGPIQLYTASFDASWEIDLFGGTRRAIEA
HCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCHHHHHH
ASAQAEAVDADLADTQVSLAAEVANAYVGLRDQQQRLALSRRTAELQQRMLELTLQRRDR
HHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GVVDDADIERLTTQVENTRATLIPLDAQVTESLDRLAILTGRAPGTLDAQLSAPGAALPT
CCCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCC
LPATVAIGDAGALLKQRPDIRAAERRLASSNAQIGERVADYFPKVTLFGDLGFSASDPGH
CCCEEEECCCHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHCCCEEEEECCCCCCCCCHH
LLRKQNYSWIGAPYLQWNLFDFGRTRGAVRAAEAARDEAEANYTKAVLAALQDANTALSR
HHHHCCCCEECCCEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGHQRDHVVALAKIETSATRSAALMNQRYRAGVSSLIDLLDTQRAALSARQNVIAGQAEL
CCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDDYISLQKSLGLGWQTDATAPQVPADAAAGAKPAG
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]