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The map label for this gene is citH [H]

Identifier: 238028550

GI number: 238028550

Start: 3419262

End: 3420557

Strand: Reverse

Name: citH [H]

Synonym: bglu_1g30140

Alternate gene names: 238028550

Gene position: 3420557-3419262 (Counterclockwise)

Preceding gene: 238028551

Following gene: 238028549

Centisome position: 87.56

GC content: 67.44

Gene sequence:

>1296_bases
ATGTCAGCAGTCATCCAAGTCGAGCGGCGCGATTCGCGCGCGCGTTCGGTGTTTCGCGCCGTCAGCGGCAATTTCCTGGA
GATGTACGACTTCACCGTCTACGGCTATTACGCGGCCGCGATCGCGGCGACGTTCTTCCCGTCCGGCAACGAGTTCGTTT
CGCTGATGCTGGCGTTCTCCGTGTTCGGCGCGGGCTTCCTGATGCGCCCGATCGGCGCGATCGTGCTGGGCGCCTACATG
GACCGCCACGGCCGCCGCAAGGGGCTGATCCTGTCGCTCTCGCTGATGGCGGTGGGCACCATGTGCGTGGCGTTCGTGCC
CGGCTACCAGAGCATCGGCCTGGTGGCGCCGGCCATCGTGCTGTTCGGCCGGCTGCTGCAGGGCTTCTCGGCCGGCGCCG
AACTCGGCGGCGTGTCGGTCTACCTGGCCGAAATCGCCTCGCGCGGCAACCGCGGCTTCTTCTGCTCGTGGCAGTCGGCC
AGCCAGCAGGTGGCGGTGGTGTTCGCGGCCTCGCTCGGCGTGCTGCTCAACGCGTTCGTGCCCGCCCAGCAGATGACGAG
CTGGGGCTGGCGCGTGCCGTTCCTGGTCGGCTGCCTGATCGTGCCGTTCCTGTTCTACATCCGCCGCACGCTGCAGGAGT
CGGACGAATTCGCCGCGCGCAAGCACCATCCCAGCATCGGCGAGATCACCCGCACCATCGTCGCCAACGCCGGGCTGATC
CTGGCCGGCATGGGCCTGGTGATCATGACCACCACCTCGTTCTACCTGATCACCGCCTATACGCCCACCTTCGGCAAGGT
GGTGCTGAAGATGTCGTCGTTCGACGCGCTGCTCGTCACGGCCTGCGTGGGCGTCTCGAACTTCATCTGGCTGCCGATCT
CGGGCGCGGTGTCCGACCGCGTGGGCCGGCGCCCGGTGATGCTGGCGTTCACCATCCTGACCGTGTTTACCGCCTATCCG
GCGGTGCAGTGGCTGATCCACGCGCCGTCGTTCGGCAAGCTGCTGGCCGTGGAGCTGTGGCTTTCGCTGCTCTATGCGTG
GTACAACGGCGCGATGGTGGTGGCGCTGACCGAGCTGATGCCGGCCAAGGTGCGCTCCACCGGCTTCTCGATGGCCTATA
GTCTGGCCACGATGGTCGGCGGCTTCACGCCGGCGATCTCCACCTGGCTGATCCATGCCACCGGCGACAAGGCCGCACCG
GGCGCGTGGATGGGCGCGGCCGCCGTGTGCGGCATGGTGGCGACGCTGCTGCTGTACCGGACGAAATCCGCCCGCGAACA
GTATCGGCTCGCCTGA

Upstream 100 bases:

>100_bases
GAGCTGCCCGCTTCGTCGTCGTTTCATCGTTTTTCTTCGCCGTGGAGGCGGCCCGCCGGGGCCGCCGCGGCAACAGGCGC
ACAGCGCAAGGAAGGTCCCC

Downstream 100 bases:

>100_bases
TTCGAATCGTAGCGGGAGGGCGCGTGAGGCTGCTGCTCGTCGAGGACAATGAGGAGCTGGCGCACTGGCTGGCGATGACG
CTGACGCACGACGGCTTCAT

Product: citrate-proton symporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MSAVIQVERRDSRARSVFRAVSGNFLEMYDFTVYGYYAAAIAATFFPSGNEFVSLMLAFSVFGAGFLMRPIGAIVLGAYM
DRHGRRKGLILSLSLMAVGTMCVAFVPGYQSIGLVAPAIVLFGRLLQGFSAGAELGGVSVYLAEIASRGNRGFFCSWQSA
SQQVAVVFAASLGVLLNAFVPAQQMTSWGWRVPFLVGCLIVPFLFYIRRTLQESDEFAARKHHPSIGEITRTIVANAGLI
LAGMGLVIMTTTSFYLITAYTPTFGKVVLKMSSFDALLVTACVGVSNFIWLPISGAVSDRVGRRPVMLAFTILTVFTAYP
AVQWLIHAPSFGKLLAVELWLSLLYAWYNGAMVVALTELMPAKVRSTGFSMAYSLATMVGGFTPAISTWLIHATGDKAAP
GAWMGAAAVCGMVATLLLYRTKSAREQYRLA

Sequences:

>Translated_431_residues
MSAVIQVERRDSRARSVFRAVSGNFLEMYDFTVYGYYAAAIAATFFPSGNEFVSLMLAFSVFGAGFLMRPIGAIVLGAYM
DRHGRRKGLILSLSLMAVGTMCVAFVPGYQSIGLVAPAIVLFGRLLQGFSAGAELGGVSVYLAEIASRGNRGFFCSWQSA
SQQVAVVFAASLGVLLNAFVPAQQMTSWGWRVPFLVGCLIVPFLFYIRRTLQESDEFAARKHHPSIGEITRTIVANAGLI
LAGMGLVIMTTTSFYLITAYTPTFGKVVLKMSSFDALLVTACVGVSNFIWLPISGAVSDRVGRRPVMLAFTILTVFTAYP
AVQWLIHAPSFGKLLAVELWLSLLYAWYNGAMVVALTELMPAKVRSTGFSMAYSLATMVGGFTPAISTWLIHATGDKAAP
GAWMGAAAVCGMVATLLLYRTKSAREQYRLA
>Mature_430_residues
SAVIQVERRDSRARSVFRAVSGNFLEMYDFTVYGYYAAAIAATFFPSGNEFVSLMLAFSVFGAGFLMRPIGAIVLGAYMD
RHGRRKGLILSLSLMAVGTMCVAFVPGYQSIGLVAPAIVLFGRLLQGFSAGAELGGVSVYLAEIASRGNRGFFCSWQSAS
QQVAVVFAASLGVLLNAFVPAQQMTSWGWRVPFLVGCLIVPFLFYIRRTLQESDEFAARKHHPSIGEITRTIVANAGLIL
AGMGLVIMTTTSFYLITAYTPTFGKVVLKMSSFDALLVTACVGVSNFIWLPISGAVSDRVGRRPVMLAFTILTVFTAYPA
VQWLIHAPSFGKLLAVELWLSLLYAWYNGAMVVALTELMPAKVRSTGFSMAYSLATMVGGFTPAISTWLIHATGDKAAPG
AWMGAAAVCGMVATLLLYRTKSAREQYRLA

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=427, Percent_Identity=28.5714285714286, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI1788942, Length=427, Percent_Identity=29.9765807962529, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1788292, Length=429, Percent_Identity=27.972027972028, Blast_Score=165, Evalue=6e-42,
Organism=Escherichia coli, GI1789941, Length=422, Percent_Identity=30.0947867298578, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI87082231, Length=216, Percent_Identity=28.2407407407407, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46547; Mature: 46416

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHCCC
>Mature Secondary Structure 
SAVIQVERRDSRARSVFRAVSGNFLEMYDFTVYGYYAAAIAATFFPSGNEFVSLMLAFS
CCEEEEHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
VFGAGFLMRPIGAIVLGAYMDRHGRRKGLILSLSLMAVGTMCVAFVPGYQSIGLVAPAIV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LFGRLLQGFSAGAELGGVSVYLAEIASRGNRGFFCSWQSASQQVAVVFAASLGVLLNAFV
HHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHC
PAQQMTSWGWRVPFLVGCLIVPFLFYIRRTLQESDEFAARKHHPSIGEITRTIVANAGLI
CHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHH
LAGMGLVIMTTTSFYLITAYTPTFGKVVLKMSSFDALLVTACVGVSNFIWLPISGAVSDR
HHHCCHHEEEECCEEEEEEECCCHHHHHHHHCCCHHHHHHHHHHHCCEEEEECCCHHHHH
VGRRPVMLAFTILTVFTAYPAVQWLIHAPSFGKLLAVELWLSLLYAWYNGAMVVALTELM
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHH
PAKVRSTGFSMAYSLATMVGGFTPAISTWLIHATGDKAAPGAWMGAAAVCGMVATLLLYR
HHHHHHHHHHHHHHHHHHHCCCCHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
TKSAREQYRLA
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]