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The map label for this gene is pykA [H]

Identifier: 238028513

GI number: 238028513

Start: 3371392

End: 3372828

Strand: Reverse

Name: pykA [H]

Synonym: bglu_1g29770

Alternate gene names: 238028513

Gene position: 3372828-3371392 (Counterclockwise)

Preceding gene: 238028514

Following gene: 238028512

Centisome position: 86.34

GC content: 68.13

Gene sequence:

>1437_bases
ATGCTTCGCGCCACCAAGATAGTCGCCACGATCGGCCCGGCTTCCAGTTCGCCCGAGATTCTGCTGCAGATGATTCAGGC
GGGCCTCGACGTCGTGCGGCTCAACTTTTCGCACGGCACCGCCGACGACCACCGCCAGCGCGCCGAGATGGTCCGCGAGG
CCGCGCGCAAGGTCGGCCGGGAAATCGCGATCATGGCGGACCTGCAGGGCCCGAAGATCCGCGTCGGCCGGTTCGAGAAC
GGCAGGACCACGCTCGCGCCGGGGCAGCCGTTCATCCTCGACGCCACCTGCGAACTCGGCAACGACGAGCGCGTCGGCCT
CGACTACAAGGACCTGCCGCGCGACCTGAAGGCGGGCGACGTGCTGCTGCTCAACGACGGCCTGATCGTGCTGACCGTCG
AGCGCGTGCTCGGCGACGAGATCCACACCATCGTCAAGGTGGGCGGCGAGCTCTCGAACAACAAGGGCATCAACCGGCAG
GGCGGCGGGCTGTCGGCGCCGGCGCTGACCGCGAAGGACATGGAGGACATCCGCACGGCGATGTCGCTCGGCGCGGACCT
GGTGGCGGTGTCGTTCCCGAAGAACGCCACCGACATGGAAATGGCGCGCCAGCTCGCGAACATCGCGGGCGCGCCCTACG
GCATCAAGCCGAAGATGATCGCGAAGATCGAGCGCGCCGAGGCGATCCCGGCGCTGCAGGACATCCTCGACGCCTCGGAC
GGCATCATGGTGGCGCGCGGCGACCTGGCCGTCGAAGTCGGCAACGCGGCCGTGCCGGCGCTGCAAAAGCGCATGATCCG
CATGGCGCGCGAGTCGAACAAGCTGGTGATCACGGCCACCCAGATGATGGAATCGATGATCCACGCGCCGGTGCCCACCC
GCGCCGAGGTGTCGGACGTCGCCAACGCCGTGCTCGACGGCACCGACGCCGTGATGCTGTCGGCCGAATCGGCCGCGGGC
AAGTACCCGGTCGTGACGATCGAGACCATGGCCGCGATCTGCGTCGAGGCCGAGAAGTCCGAGCAGGTCGAGCTCGACCG
CGATTTCCTCGACCGCACCTTCACGCGCATCGACCAGTCGATCGCGATGGGGGCGCTCTTCACCGCCTACCACCTCGGCG
CCAAGGCGATCATCGCGCTGACCGAGTCGGGCTCGACCGCGCTCTGGATGTCGCGCCACTACACGCACGTGCCGATCTTC
GCGCTGACGCCGCGGGTGGGCAGCGAGCGCACCATGGCGCTGTTCCGCAACGTCACGCCGCTGCACGTCGATTTCAACAG
CGACCGCGATTCGGCGATGCAGGCCGCCATCGAGATCGTGATGCGGCAGGGCTACGTGGGGCTCGGCGACATGGTGGTGC
TCACCGTCGGCGAACCGATGGGCCAGGCGGGCGGTACCAATACGCTCAAGATCGTGCGCGTCGGCGAGACGTTCTGA

Upstream 100 bases:

>100_bases
CGGCGCGCGCACCGGAGCACCGGCCGCTGGGCCCGGCGGCTCCGGCTCTCGCAGCAGTATCTCCAGCGCTTCCTCCACCC
AGACCATCATGAGGAGTTTC

Downstream 100 bases:

>100_bases
TCGATCTGTCCCGATATTTCGGGCCATAAAAGCCGCGCGGGGTCGAACGCCCCGCGCGGCTTTTTTCGCATTTTTGCCGC
GATGGCCGGCACGGAATTCG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MLRATKIVATIGPASSSPEILLQMIQAGLDVVRLNFSHGTADDHRQRAEMVREAARKVGREIAIMADLQGPKIRVGRFEN
GRTTLAPGQPFILDATCELGNDERVGLDYKDLPRDLKAGDVLLLNDGLIVLTVERVLGDEIHTIVKVGGELSNNKGINRQ
GGGLSAPALTAKDMEDIRTAMSLGADLVAVSFPKNATDMEMARQLANIAGAPYGIKPKMIAKIERAEAIPALQDILDASD
GIMVARGDLAVEVGNAAVPALQKRMIRMARESNKLVITATQMMESMIHAPVPTRAEVSDVANAVLDGTDAVMLSAESAAG
KYPVVTIETMAAICVEAEKSEQVELDRDFLDRTFTRIDQSIAMGALFTAYHLGAKAIIALTESGSTALWMSRHYTHVPIF
ALTPRVGSERTMALFRNVTPLHVDFNSDRDSAMQAAIEIVMRQGYVGLGDMVVLTVGEPMGQAGGTNTLKIVRVGETF

Sequences:

>Translated_478_residues
MLRATKIVATIGPASSSPEILLQMIQAGLDVVRLNFSHGTADDHRQRAEMVREAARKVGREIAIMADLQGPKIRVGRFEN
GRTTLAPGQPFILDATCELGNDERVGLDYKDLPRDLKAGDVLLLNDGLIVLTVERVLGDEIHTIVKVGGELSNNKGINRQ
GGGLSAPALTAKDMEDIRTAMSLGADLVAVSFPKNATDMEMARQLANIAGAPYGIKPKMIAKIERAEAIPALQDILDASD
GIMVARGDLAVEVGNAAVPALQKRMIRMARESNKLVITATQMMESMIHAPVPTRAEVSDVANAVLDGTDAVMLSAESAAG
KYPVVTIETMAAICVEAEKSEQVELDRDFLDRTFTRIDQSIAMGALFTAYHLGAKAIIALTESGSTALWMSRHYTHVPIF
ALTPRVGSERTMALFRNVTPLHVDFNSDRDSAMQAAIEIVMRQGYVGLGDMVVLTVGEPMGQAGGTNTLKIVRVGETF
>Mature_478_residues
MLRATKIVATIGPASSSPEILLQMIQAGLDVVRLNFSHGTADDHRQRAEMVREAARKVGREIAIMADLQGPKIRVGRFEN
GRTTLAPGQPFILDATCELGNDERVGLDYKDLPRDLKAGDVLLLNDGLIVLTVERVLGDEIHTIVKVGGELSNNKGINRQ
GGGLSAPALTAKDMEDIRTAMSLGADLVAVSFPKNATDMEMARQLANIAGAPYGIKPKMIAKIERAEAIPALQDILDASD
GIMVARGDLAVEVGNAAVPALQKRMIRMARESNKLVITATQMMESMIHAPVPTRAEVSDVANAVLDGTDAVMLSAESAAG
KYPVVTIETMAAICVEAEKSEQVELDRDFLDRTFTRIDQSIAMGALFTAYHLGAKAIIALTESGSTALWMSRHYTHVPIF
ALTPRVGSERTMALFRNVTPLHVDFNSDRDSAMQAAIEIVMRQGYVGLGDMVVLTVGEPMGQAGGTNTLKIVRVGETF

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI32967597, Length=496, Percent_Identity=37.7016129032258, Blast_Score=277, Evalue=1e-74,
Organism=Homo sapiens, GI33286422, Length=496, Percent_Identity=36.2903225806452, Blast_Score=277, Evalue=2e-74,
Organism=Homo sapiens, GI33286420, Length=496, Percent_Identity=36.2903225806452, Blast_Score=277, Evalue=2e-74,
Organism=Homo sapiens, GI10835121, Length=496, Percent_Identity=37.7016129032258, Blast_Score=277, Evalue=2e-74,
Organism=Homo sapiens, GI33286418, Length=493, Percent_Identity=36.105476673428, Blast_Score=272, Evalue=4e-73,
Organism=Homo sapiens, GI310128732, Length=291, Percent_Identity=36.426116838488, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI310128730, Length=291, Percent_Identity=36.426116838488, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI310128736, Length=239, Percent_Identity=33.8912133891213, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI310128734, Length=239, Percent_Identity=33.8912133891213, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI310128738, Length=218, Percent_Identity=31.651376146789, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1788160, Length=482, Percent_Identity=51.8672199170125, Blast_Score=454, Evalue=1e-129,
Organism=Escherichia coli, GI1787965, Length=472, Percent_Identity=38.771186440678, Blast_Score=304, Evalue=9e-84,
Organism=Caenorhabditis elegans, GI17544584, Length=488, Percent_Identity=36.4754098360656, Blast_Score=282, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI71984413, Length=489, Percent_Identity=38.6503067484663, Blast_Score=265, Evalue=4e-71,
Organism=Caenorhabditis elegans, GI17506829, Length=489, Percent_Identity=38.6503067484663, Blast_Score=265, Evalue=4e-71,
Organism=Caenorhabditis elegans, GI71984406, Length=489, Percent_Identity=38.6503067484663, Blast_Score=265, Evalue=4e-71,
Organism=Caenorhabditis elegans, GI17506831, Length=489, Percent_Identity=38.6503067484663, Blast_Score=265, Evalue=4e-71,
Organism=Saccharomyces cerevisiae, GI6319279, Length=488, Percent_Identity=34.8360655737705, Blast_Score=263, Evalue=4e-71,
Organism=Saccharomyces cerevisiae, GI6324923, Length=493, Percent_Identity=35.6997971602434, Blast_Score=254, Evalue=2e-68,
Organism=Drosophila melanogaster, GI24648964, Length=493, Percent_Identity=37.525354969574, Blast_Score=277, Evalue=1e-74,
Organism=Drosophila melanogaster, GI28571814, Length=493, Percent_Identity=37.525354969574, Blast_Score=277, Evalue=1e-74,
Organism=Drosophila melanogaster, GI24648966, Length=465, Percent_Identity=36.989247311828, Blast_Score=240, Evalue=1e-63,
Organism=Drosophila melanogaster, GI24581235, Length=488, Percent_Identity=31.9672131147541, Blast_Score=222, Evalue=5e-58,
Organism=Drosophila melanogaster, GI24646914, Length=272, Percent_Identity=36.3970588235294, Blast_Score=144, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51355; Mature: 51355

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRATKIVATIGPASSSPEILLQMIQAGLDVVRLNFSHGTADDHRQRAEMVREAARKVGR
CCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
EIAIMADLQGPKIRVGRFENGRTTLAPGQPFILDATCELGNDERVGLDYKDLPRDLKAGD
EEEEEEECCCCEEEEEEECCCCEEECCCCCEEEEEEECCCCCCCCCCCHHHCCCCCCCCC
VLLLNDGLIVLTVERVLGDEIHTIVKVGGELSNNKGINRQGGGLSAPALTAKDMEDIRTA
EEEEECCEEEEEEHHHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
MSLGADLVAVSFPKNATDMEMARQLANIAGAPYGIKPKMIAKIERAEAIPALQDILDASD
HHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
GIMVARGDLAVEVGNAAVPALQKRMIRMARESNKLVITATQMMESMIHAPVPTRAEVSDV
CEEEEECCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCCCHHHHHH
ANAVLDGTDAVMLSAESAAGKYPVVTIETMAAICVEAEKSEQVELDRDFLDRTFTRIDQS
HHHHHCCCCEEEEEECCCCCCCCEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
IAMGALFTAYHLGAKAIIALTESGSTALWMSRHYTHVPIFALTPRVGSERTMALFRNVTP
HHHHHHHHHHHCCCEEEEEEECCCCEEEEEECCCCEEEEEEECCCCCCHHHHHHHHCCCE
LHVDFNSDRDSAMQAAIEIVMRQGYVGLGDMVVLTVGEPMGQAGGTNTLKIVRVGETF
EEEECCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCEEEEEEECCCC
>Mature Secondary Structure
MLRATKIVATIGPASSSPEILLQMIQAGLDVVRLNFSHGTADDHRQRAEMVREAARKVGR
CCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
EIAIMADLQGPKIRVGRFENGRTTLAPGQPFILDATCELGNDERVGLDYKDLPRDLKAGD
EEEEEEECCCCEEEEEEECCCCEEECCCCCEEEEEEECCCCCCCCCCCHHHCCCCCCCCC
VLLLNDGLIVLTVERVLGDEIHTIVKVGGELSNNKGINRQGGGLSAPALTAKDMEDIRTA
EEEEECCEEEEEEHHHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
MSLGADLVAVSFPKNATDMEMARQLANIAGAPYGIKPKMIAKIERAEAIPALQDILDASD
HHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
GIMVARGDLAVEVGNAAVPALQKRMIRMARESNKLVITATQMMESMIHAPVPTRAEVSDV
CEEEEECCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCCCHHHHHH
ANAVLDGTDAVMLSAESAAGKYPVVTIETMAAICVEAEKSEQVELDRDFLDRTFTRIDQS
HHHHHCCCCEEEEEECCCCCCCCEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
IAMGALFTAYHLGAKAIIALTESGSTALWMSRHYTHVPIFALTPRVGSERTMALFRNVTP
HHHHHHHHHHHCCCEEEEEEECCCCEEEEEECCCCEEEEEEECCCCCCHHHHHHHHCCCE
LHVDFNSDRDSAMQAAIEIVMRQGYVGLGDMVVLTVGEPMGQAGGTNTLKIVRVGETF
EEEECCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12522265 [H]