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The map label for this gene is flhA [H]

Identifier: 238028494

GI number: 238028494

Start: 3346622

End: 3347728

Strand: Reverse

Name: flhA [H]

Synonym: bglu_1g29580

Alternate gene names: 238028494

Gene position: 3347728-3346622 (Counterclockwise)

Preceding gene: 238028496

Following gene: 238028493

Centisome position: 85.7

GC content: 65.49

Gene sequence:

>1107_bases
ATGAAGACGAAAGCCGCGATCGCATGGAAGGCCGGTGCGCCGCTGACCATCGAGGAAGTGGATCTGGAAGGCCCGCGCGC
GGGCGAGGTGCTGATCGAGGTGAAGGCCACGGGGATCTGCCATACCGACTACTACACGCTGTCGGGCGCCGATCCGGAAG
GCATCTTCCCGGCGATCCTCGGCCACGAGGGCGCGGGCGTGGTGGTGGACGTCGGCCCCGGCGTGGGCACGCTCAGAAAG
GGCGACCACGTGATTCCGCTCTACACGCCCGAATGCCGCGAGTGCAAGTTCTGCCTGTCGCGCAAGACCAACCTGTGCCA
GAAGATCCGCGCCACGCAGGGCAAGGGCCTGATGCCGGACGCCACCTCGCGCTTCTCGCTCGACGGCAAGCCGATCTTCC
ACTACATGGGCACCTCGACGTTCTCGAACTACATCGTCGTGCCGGAGATCGCGGTGGCGAAGATCCGCGAGGATGCGCCG
TTCGACAAGGTCTGCTACATCGGCTGCGGCGTGACGACGGGGGTGGGCGCCGTGGTCTACTCGGCGAAGGTCGAGGCCGG
CGCGAACGTGGTGGTGTTCGGCCTCGGCGGGATCGGCCTGAACGTGATCCAGGGCGCGAAGATGGTGGGCGCCGACAAGA
TCATCGGCATCGATATCAACCCGGCGCGCGTCGAACTGGCGAAGAAGTTCGGCATGACGCACTTCATCAACCCGAAGGAG
GTCGAGAACGTGGTCGACCAGATCGTGCAGCTGACCGACGGCGGCGCGGATTACTCCTTCGAGTGCATCGGCAACGTCAC
CACCATGCGTCAGGCGCTCGAATGCACCCACAAGGGCTGGGGCCAGTCCTTCATCATCGGCGTGGCGGCGGCCGGCCAGG
AGATCAGCACGCGGCCGTTCCAGCTCGTCACGGGCCGCGAATGGAAGGGCTCGGCGTTCGGCGGCGCGCGCGGGCGCACC
GACGTGCCGAAGATCGTCGACTGGTACATGGAAGGCAAGCTCAACATCGACGACCTGATCACGCACCGTCTGCCGCTCGA
GCAGATCAACGACGGCTTCGACCTGATGAAGAAGGGCGAGTCGATCCGCTCGGTCGTGCTCTACTGA

Upstream 100 bases:

>100_bases
GCGCGCCGCATGCCCGCCCCGGGCCCGGCGTCGGCCGCCGCGCGCCGCCGTGCGCGGCGAGCAAGCCCGCAACGTCACAG
CACAGACAAGGAGCATCACG

Downstream 100 bases:

>100_bases
TCCGGAGGCAGCGATCATGCTTGAACTGACTGCGTCGCATGCATGCTTCGGCGGCGAGCAGCGTTTCTATCGCCACGAAT
CGTCGATCATCGGCCTGCCG

Product: zinc-containing alcohol dehydrogenase superfamily

Products: NA

Alternate protein names: Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MKTKAAIAWKAGAPLTIEEVDLEGPRAGEVLIEVKATGICHTDYYTLSGADPEGIFPAILGHEGAGVVVDVGPGVGTLRK
GDHVIPLYTPECRECKFCLSRKTNLCQKIRATQGKGLMPDATSRFSLDGKPIFHYMGTSTFSNYIVVPEIAVAKIREDAP
FDKVCYIGCGVTTGVGAVVYSAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGIDINPARVELAKKFGMTHFINPKE
VENVVDQIVQLTDGGADYSFECIGNVTTMRQALECTHKGWGQSFIIGVAAAGQEISTRPFQLVTGREWKGSAFGGARGRT
DVPKIVDWYMEGKLNIDDLITHRLPLEQINDGFDLMKKGESIRSVVLY

Sequences:

>Translated_368_residues
MKTKAAIAWKAGAPLTIEEVDLEGPRAGEVLIEVKATGICHTDYYTLSGADPEGIFPAILGHEGAGVVVDVGPGVGTLRK
GDHVIPLYTPECRECKFCLSRKTNLCQKIRATQGKGLMPDATSRFSLDGKPIFHYMGTSTFSNYIVVPEIAVAKIREDAP
FDKVCYIGCGVTTGVGAVVYSAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGIDINPARVELAKKFGMTHFINPKE
VENVVDQIVQLTDGGADYSFECIGNVTTMRQALECTHKGWGQSFIIGVAAAGQEISTRPFQLVTGREWKGSAFGGARGRT
DVPKIVDWYMEGKLNIDDLITHRLPLEQINDGFDLMKKGESIRSVVLY
>Mature_368_residues
MKTKAAIAWKAGAPLTIEEVDLEGPRAGEVLIEVKATGICHTDYYTLSGADPEGIFPAILGHEGAGVVVDVGPGVGTLRK
GDHVIPLYTPECRECKFCLSRKTNLCQKIRATQGKGLMPDATSRFSLDGKPIFHYMGTSTFSNYIVVPEIAVAKIREDAP
FDKVCYIGCGVTTGVGAVVYSAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGIDINPARVELAKKFGMTHFINPKE
VENVVDQIVQLTDGGADYSFECIGNVTTMRQALECTHKGWGQSFIIGVAAAGQEISTRPFQLVTGREWKGSAFGGARGRT
DVPKIVDWYMEGKLNIDDLITHRLPLEQINDGFDLMKKGESIRSVVLY

Specific function: Essential for the oxidation of formaldehyde produced during methylotrophic growth [H]

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI71565154, Length=367, Percent_Identity=61.0354223433242, Blast_Score=456, Evalue=1e-128,
Organism=Homo sapiens, GI71565152, Length=374, Percent_Identity=47.5935828877005, Blast_Score=366, Evalue=1e-101,
Organism=Homo sapiens, GI34577061, Length=369, Percent_Identity=46.8834688346883, Blast_Score=355, Evalue=5e-98,
Organism=Homo sapiens, GI4501933, Length=369, Percent_Identity=46.6124661246612, Blast_Score=350, Evalue=8e-97,
Organism=Homo sapiens, GI4501929, Length=369, Percent_Identity=46.3414634146341, Blast_Score=345, Evalue=3e-95,
Organism=Homo sapiens, GI262073058, Length=369, Percent_Identity=44.9864498644986, Blast_Score=340, Evalue=1e-93,
Organism=Homo sapiens, GI71743840, Length=369, Percent_Identity=44.9864498644986, Blast_Score=340, Evalue=1e-93,
Organism=Homo sapiens, GI156523966, Length=368, Percent_Identity=44.0217391304348, Blast_Score=332, Evalue=2e-91,
Organism=Homo sapiens, GI4501939, Length=361, Percent_Identity=44.0443213296399, Blast_Score=325, Evalue=4e-89,
Organism=Escherichia coli, GI1786552, Length=369, Percent_Identity=58.8075880758808, Blast_Score=438, Evalue=1e-124,
Organism=Escherichia coli, GI87082125, Length=379, Percent_Identity=27.4406332453826, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1790045, Length=376, Percent_Identity=27.9255319148936, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1788073, Length=361, Percent_Identity=26.3157894736842, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1786825, Length=378, Percent_Identity=24.8677248677249, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI87081918, Length=342, Percent_Identity=28.9473684210526, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI226510992, Length=372, Percent_Identity=25.2688172043011, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1787863, Length=347, Percent_Identity=25.9365994236311, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788075, Length=370, Percent_Identity=27.2972972972973, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788407, Length=351, Percent_Identity=25.6410256410256, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1790718, Length=326, Percent_Identity=23.9263803680982, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786518, Length=367, Percent_Identity=24.5231607629428, Blast_Score=66, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI25146526, Length=373, Percent_Identity=56.5683646112601, Blast_Score=444, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI71997431, Length=375, Percent_Identity=56.2666666666667, Blast_Score=440, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI17565904, Length=337, Percent_Identity=57.566765578635, Blast_Score=411, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17562876, Length=355, Percent_Identity=27.887323943662, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17562584, Length=367, Percent_Identity=26.7029972752044, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71988145, Length=373, Percent_Identity=25.7372654155496, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17562582, Length=330, Percent_Identity=25.1515151515152, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17562878, Length=358, Percent_Identity=24.5810055865922, Blast_Score=78, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6320033, Length=371, Percent_Identity=54.4474393530997, Blast_Score=432, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6323729, Length=365, Percent_Identity=28.4931506849315, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6323099, Length=371, Percent_Identity=28.5714285714286, Blast_Score=100, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6319621, Length=372, Percent_Identity=27.6881720430108, Blast_Score=100, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6324486, Length=371, Percent_Identity=26.9541778975741, Blast_Score=100, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6323961, Length=364, Percent_Identity=27.1978021978022, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6322619, Length=363, Percent_Identity=28.9256198347107, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6319955, Length=363, Percent_Identity=28.9256198347107, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6319257, Length=366, Percent_Identity=26.5027322404372, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319258, Length=272, Percent_Identity=27.9411764705882, Blast_Score=73, Evalue=7e-14,
Organism=Drosophila melanogaster, GI17737895, Length=368, Percent_Identity=62.2282608695652, Blast_Score=485, Evalue=1e-137,
Organism=Drosophila melanogaster, GI17137530, Length=341, Percent_Identity=28.1524926686217, Blast_Score=98, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.284 [H]

Molecular weight: Translated: 39555; Mature: 39555

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKAAIAWKAGAPLTIEEVDLEGPRAGEVLIEVKATGICHTDYYTLSGADPEGIFPAIL
CCCCEEEEEECCCCEEEEEECCCCCCCCEEEEEEEECEEEECCEEEECCCCCCCCCHHHC
GHEGAGVVVDVGPGVGTLRKGDHVIPLYTPECRECKFCLSRKTNLCQKIRATQGKGLMPD
CCCCCEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
ATSRFSLDGKPIFHYMGTSTFSNYIVVPEIAVAKIREDAPFDKVCYIGCGVTTGVGAVVY
CCCEEECCCCEEEEEECCCCCCCEEEECCHHHHHHHCCCCCCCEEEEECCCHHCCCCEEE
SAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGIDINPARVELAKKFGMTHFINPKE
EEEECCCCCEEEEECCCCCHHHHCCCEEECCCEEEEECCCHHHHHHHHHHCCEECCCHHH
VENVVDQIVQLTDGGADYSFECIGNVTTMRQALECTHKGWGQSFIIGVAAAGQEISTRPF
HHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCE
QLVTGREWKGSAFGGARGRTDVPKIVDWYMEGKLNIDDLITHRLPLEQINDGFDLMKKGE
EEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHCCCHHHHCCCHHHHHCCC
SIRSVVLY
CHHHEECC
>Mature Secondary Structure
MKTKAAIAWKAGAPLTIEEVDLEGPRAGEVLIEVKATGICHTDYYTLSGADPEGIFPAIL
CCCCEEEEEECCCCEEEEEECCCCCCCCEEEEEEEECEEEECCEEEECCCCCCCCCHHHC
GHEGAGVVVDVGPGVGTLRKGDHVIPLYTPECRECKFCLSRKTNLCQKIRATQGKGLMPD
CCCCCEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
ATSRFSLDGKPIFHYMGTSTFSNYIVVPEIAVAKIREDAPFDKVCYIGCGVTTGVGAVVY
CCCEEECCCCEEEEEECCCCCCCEEEECCHHHHHHHCCCCCCCEEEEECCCHHCCCCEEE
SAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGIDINPARVELAKKFGMTHFINPKE
EEEECCCCCEEEEECCCCCHHHHCCCEEECCCEEEEECCCHHHHHHHHHHCCEECCCHHH
VENVVDQIVQLTDGGADYSFECIGNVTTMRQALECTHKGWGQSFIIGVAAAGQEISTRPF
HHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCE
QLVTGREWKGSAFGGARGRTDVPKIVDWYMEGKLNIDDLITHRLPLEQINDGFDLMKKGE
EEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHCCCHHHHCCCHHHHHCCC
SIRSVVLY
CHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7798140 [H]