Click here to switch to the map view.

The map label for this gene is yieH [H]

Identifier: 238028482

GI number: 238028482

Start: 3335018

End: 3335737

Strand: Reverse

Name: yieH [H]

Synonym: bglu_1g29460

Alternate gene names: 238028482

Gene position: 3335737-3335018 (Counterclockwise)

Preceding gene: 238028483

Following gene: 238028481

Centisome position: 85.39

GC content: 75.69

Gene sequence:

>720_bases
ATGAGCGCGAGCGACATGCGCCTGCCCGCCGGCGAGGGCCGCGACCGCCTGCTGATCTGCGATTGCGACGGCGTGCTGAT
CGACAGCGAGGCGGTGGCCGCGCGCGTGCTGGTGAGCGAGCTGGAGGCGCGCTGGCCCGGCGTGGACGTGCGCCCGGTGG
TGCTGCCGCTGCTCGGGCTGCGCACCGAGTGCCTGCTCGAGCGCGCGGCCGGCCAGGTCGGCCGCGCGCTTGCGGGCGCC
GAGATCGCGGCGATCCGCGCGGCGGTGGAGCGCGCGGCGGTGCTCGCGCCGGCCGTGGACGGCATCGACGCGGCGCTCGC
CGCGATCGCGCTGCCGATGGCCTGCGCCAGCAACAGCAATCGCGCCTATGTGGAGGCGGCGCTCACGCGCACCGGCCTCA
AGCCCTACTTCGGCGAGCGGCTGTTCTGCGCCGACGGCGTTGAACGGCCCAAGCCCGCGCCCGACGTCTATCTGGCCGCC
GCCCACACGCTCGGCTTCGCGCCCGAGCACTGCCTGGTGGTGGAGGACAGCGCCACCGGCATCACCGCCGCGGCCGCGGC
CGGGATGGCCGTGATCGGCTTCATCGGCGGCGGCCATGCCTCGCCGTCGCAGGTCGATGCGCTGCGCGCGGTCGGCGCGC
GCCGCGTGTTCGACCGGATGGACGCCTTGCCCGGCCTGGCCGCGCAGTGGATCGAGACCGGCAGCGTGCCGTTGCAGTAG

Upstream 100 bases:

>100_bases
AGCTGTCGGCGGCCTCGCTGCTGGCCGTGGCGCCGATCCTGATCGTCGGCTGGCTGTCGCAGAAGCAACTGGTGCGCGGC
CTCACGTTCGGAGCGGTCAA

Downstream 100 bases:

>100_bases
GCGAAACGAATCGCGTGCCGCGCCGGCGCCACCGGCCGCGCGGCGCGTCACGAAATCAGCGGTAAGGAAAGCGGAGACAA
ATCATGGCAAGCCTGCTTCT

Product: HAD-superfamily hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MSASDMRLPAGEGRDRLLICDCDGVLIDSEAVAARVLVSELEARWPGVDVRPVVLPLLGLRTECLLERAAGQVGRALAGA
EIAAIRAAVERAAVLAPAVDGIDAALAAIALPMACASNSNRAYVEAALTRTGLKPYFGERLFCADGVERPKPAPDVYLAA
AHTLGFAPEHCLVVEDSATGITAAAAAGMAVIGFIGGGHASPSQVDALRAVGARRVFDRMDALPGLAAQWIETGSVPLQ

Sequences:

>Translated_239_residues
MSASDMRLPAGEGRDRLLICDCDGVLIDSEAVAARVLVSELEARWPGVDVRPVVLPLLGLRTECLLERAAGQVGRALAGA
EIAAIRAAVERAAVLAPAVDGIDAALAAIALPMACASNSNRAYVEAALTRTGLKPYFGERLFCADGVERPKPAPDVYLAA
AHTLGFAPEHCLVVEDSATGITAAAAAGMAVIGFIGGGHASPSQVDALRAVGARRVFDRMDALPGLAAQWIETGSVPLQ
>Mature_238_residues
SASDMRLPAGEGRDRLLICDCDGVLIDSEAVAARVLVSELEARWPGVDVRPVVLPLLGLRTECLLERAAGQVGRALAGAE
IAAIRAAVERAAVLAPAVDGIDAALAAIALPMACASNSNRAYVEAALTRTGLKPYFGERLFCADGVERPKPAPDVYLAAA
HTLGFAPEHCLVVEDSATGITAAAAAGMAVIGFIGGGHASPSQVDALRAVGARRVFDRMDALPGLAAQWIETGSVPLQ

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates phosphoenolpyruvate and AMP [H]

COG id: COG0637

COG function: function code R; Predicted phosphatase/phosphohexomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1790151, Length=192, Percent_Identity=28.6458333333333, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1789046, Length=176, Percent_Identity=31.25, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 24617; Mature: 24485

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASDMRLPAGEGRDRLLICDCDGVLIDSEAVAARVLVSELEARWPGVDVRPVVLPLLGL
CCCCCCCCCCCCCCCEEEEECCCCEEECHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
RTECLLERAAGQVGRALAGAEIAAIRAAVERAAVLAPAVDGIDAALAAIALPMACASNSN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC
RAYVEAALTRTGLKPYFGERLFCADGVERPKPAPDVYLAAAHTLGFAPEHCLVVEDSATG
CEEHHHHHHHCCCCHHHCCEEEECCCCCCCCCCCCEEEEHHHHHCCCCCCEEEEECCCCC
ITAAAAAGMAVIGFIGGGHASPSQVDALRAVGARRVFDRMDALPGLAAQWIETGSVPLQ
HHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCC
>Mature Secondary Structure 
SASDMRLPAGEGRDRLLICDCDGVLIDSEAVAARVLVSELEARWPGVDVRPVVLPLLGL
CCCCCCCCCCCCCCEEEEECCCCEEECHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
RTECLLERAAGQVGRALAGAEIAAIRAAVERAAVLAPAVDGIDAALAAIALPMACASNSN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC
RAYVEAALTRTGLKPYFGERLFCADGVERPKPAPDVYLAAAHTLGFAPEHCLVVEDSATG
CEEHHHHHHHCCCCHHHCCEEEECCCCCCCCCCCCEEEEHHHHHCCCCCCEEEEECCCCC
ITAAAAAGMAVIGFIGGGHASPSQVDALRAVGARRVFDRMDALPGLAAQWIETGSVPLQ
HHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]