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The map label for this gene is glnG [H]

Identifier: 238028047

GI number: 238028047

Start: 2838228

End: 2839715

Strand: Reverse

Name: glnG [H]

Synonym: bglu_1g24980

Alternate gene names: 238028047

Gene position: 2839715-2838228 (Counterclockwise)

Preceding gene: 238028048

Following gene: 238028046

Centisome position: 72.69

GC content: 70.09

Gene sequence:

>1488_bases
ATGAAGCCGATCTGGATAGTAGACGACGACCAATCGATCCGCTGGGTGCTTGAAAAGGCGCTCGCACGCGACAGCTTCGC
GACCCGGAGCTTTTCGAACGTGCGCGACGCGCTGGCCGCGCTCGATCATGAATCGCCGCAGGTGCTGGTGTCCGACATCC
GCATGCCGGGCGGTTCGGGCCTCGAGCTGCTGCAGGCCATGCACGAGCGCCTGCCGGGGCTGCCCGTCATCATCATGACC
GCGTTCTCCGACCTCGACAGCGCGGTGGCCGCGTTCCAGGGCGGCGCGTTCGAATATCTGGCCAAGCCGTTCGACGTCGA
CAAGGCCATCGAGCTTATCCGGCGCGCGGTGGAGGAAAGCCTGCGCGGCGGCGTGCCCGACGACGACAAGCTCACCGAGG
CGCCCGAGATGCTCGGCCAGGCGCCCGCGATGCAGGACATGTTCCGCGCCATCGGCCGGCTCTCCCACTCGGCCGCGACC
GTGCTGATCACCGGCGAATCGGGCACCGGCAAGGAGCTCGTCGCGCGCGCGCTGCACCGTCACAGCCCGCGCTCGAACGG
GCCGTTCATCGCGCTGAACACCGCGGCGATCCCGAAGGACCTGCTGGAGTCCGAGCTGTTCGGCCATGAGCGCGGCGCGT
TCACGGGCGCGCAGACCACGCGCCAGGGCCGCTTCGAGCAGGCCGAGAACGGCACGCTGTTCCTCGACGAAATCGGCGAC
ATGCCGTTCGATCTGCAGACGCGCCTGCTGCGCGTGCTGTCGGACGGGCAGTTCTACCGCGTGGGCGGCCACAATCCGCT
GCGCGCCAACGTGCGCGTGATCGCGGCGACCCACCAGAACCTCGAATCGCGGGTGCGCCAGGGCCTGTTCCGCGAGGACT
TGTACCATCGCCTCAACGTGATCCGGCTCAGGCTGCCGCCGCTGCGCGAGCGCAGCGAGGACATCCCGCTGCTGACCAAG
CATTTCCTGCAGAAGAGCGCGCGCGATCTCGGCGTCGAGCCCAAGCGCGTGTCCGACGACGCGCTCGCCTACCTCAGCGC
GCTGCCGTTCCCCGGCAACGTGCGCCAGCTCGAGAACCTCGCGAACTGGCTGACCGTGATGGCGCCGGCGCAGACCGTCG
AGATCAAGGACCTGCCGCCCGATCTCGGTCCCGCCCAGGCCGCGCCCGGCGAGGGCGTGACGCCGGCGGCCGTGGCCGCC
AGCACCGACGCGCTGCCCAACAGCGCGACGGCCGCGGTGCCGGTGCCGGCCGCGCCCGCCGCGTCGAGCAATCCGGCCTG
GGAGCAGGGCTTGCGCAGCGAGGTGGCGCGCCTGCTGCGCGAGAATTCGGCCGACGTGATGGACGAACTCGCGCGGCGCT
TCGAGGCCGCCGTGATCCGCGAGGCGCTCGATTTCACGCGCGGGCGCAAGGTCGAGGCGGCCGAGCGGCTCGGCATCGGC
CGCAACACGATCACGCGCAAGATTCAGGAACTGCATCTGGAGCCTTGA

Upstream 100 bases:

>100_bases
CGAGGTGGAAAGCCGTCCGGGCCGCACCGAATTTCAGATTTTGCTGCCGCTCGACCATTGAGCGGCGGCGCCGCGACTCA
CTGAGACATCTGACCGACCT

Downstream 100 bases:

>100_bases
GCAGCGCGCGCCGGCCGCGGCTGGCCGGCGCGTCGTTCGGGGTCGAACGCAGCCGCCCGTGGCGCAATGCGACCACCCAT
TCGGCGGCGGCCCGGCGGCG

Product: nitrogen metabolism transcriptional regulator, NtrC, Fis Family

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MKPIWIVDDDQSIRWVLEKALARDSFATRSFSNVRDALAALDHESPQVLVSDIRMPGGSGLELLQAMHERLPGLPVIIMT
AFSDLDSAVAAFQGGAFEYLAKPFDVDKAIELIRRAVEESLRGGVPDDDKLTEAPEMLGQAPAMQDMFRAIGRLSHSAAT
VLITGESGTGKELVARALHRHSPRSNGPFIALNTAAIPKDLLESELFGHERGAFTGAQTTRQGRFEQAENGTLFLDEIGD
MPFDLQTRLLRVLSDGQFYRVGGHNPLRANVRVIAATHQNLESRVRQGLFREDLYHRLNVIRLRLPPLRERSEDIPLLTK
HFLQKSARDLGVEPKRVSDDALAYLSALPFPGNVRQLENLANWLTVMAPAQTVEIKDLPPDLGPAQAAPGEGVTPAAVAA
STDALPNSATAAVPVPAAPAASSNPAWEQGLRSEVARLLRENSADVMDELARRFEAAVIREALDFTRGRKVEAAERLGIG
RNTITRKIQELHLEP

Sequences:

>Translated_495_residues
MKPIWIVDDDQSIRWVLEKALARDSFATRSFSNVRDALAALDHESPQVLVSDIRMPGGSGLELLQAMHERLPGLPVIIMT
AFSDLDSAVAAFQGGAFEYLAKPFDVDKAIELIRRAVEESLRGGVPDDDKLTEAPEMLGQAPAMQDMFRAIGRLSHSAAT
VLITGESGTGKELVARALHRHSPRSNGPFIALNTAAIPKDLLESELFGHERGAFTGAQTTRQGRFEQAENGTLFLDEIGD
MPFDLQTRLLRVLSDGQFYRVGGHNPLRANVRVIAATHQNLESRVRQGLFREDLYHRLNVIRLRLPPLRERSEDIPLLTK
HFLQKSARDLGVEPKRVSDDALAYLSALPFPGNVRQLENLANWLTVMAPAQTVEIKDLPPDLGPAQAAPGEGVTPAAVAA
STDALPNSATAAVPVPAAPAASSNPAWEQGLRSEVARLLRENSADVMDELARRFEAAVIREALDFTRGRKVEAAERLGIG
RNTITRKIQELHLEP
>Mature_495_residues
MKPIWIVDDDQSIRWVLEKALARDSFATRSFSNVRDALAALDHESPQVLVSDIRMPGGSGLELLQAMHERLPGLPVIIMT
AFSDLDSAVAAFQGGAFEYLAKPFDVDKAIELIRRAVEESLRGGVPDDDKLTEAPEMLGQAPAMQDMFRAIGRLSHSAAT
VLITGESGTGKELVARALHRHSPRSNGPFIALNTAAIPKDLLESELFGHERGAFTGAQTTRQGRFEQAENGTLFLDEIGD
MPFDLQTRLLRVLSDGQFYRVGGHNPLRANVRVIAATHQNLESRVRQGLFREDLYHRLNVIRLRLPPLRERSEDIPLLTK
HFLQKSARDLGVEPKRVSDDALAYLSALPFPGNVRQLENLANWLTVMAPAQTVEIKDLPPDLGPAQAAPGEGVTPAAVAA
STDALPNSATAAVPVPAAPAASSNPAWEQGLRSEVARLLRENSADVMDELARRFEAAVIREALDFTRGRKVEAAERLGIG
RNTITRKIQELHLEP

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=493, Percent_Identity=56.5922920892495, Blast_Score=532, Evalue=1e-152,
Organism=Escherichia coli, GI1788550, Length=497, Percent_Identity=39.4366197183099, Blast_Score=357, Evalue=1e-99,
Organism=Escherichia coli, GI1790437, Length=485, Percent_Identity=36.9072164948454, Blast_Score=294, Evalue=9e-81,
Organism=Escherichia coli, GI1788905, Length=370, Percent_Identity=41.6216216216216, Blast_Score=281, Evalue=8e-77,
Organism=Escherichia coli, GI87082117, Length=356, Percent_Identity=40.7303370786517, Blast_Score=243, Evalue=3e-65,
Organism=Escherichia coli, GI1789233, Length=360, Percent_Identity=39.4444444444444, Blast_Score=238, Evalue=6e-64,
Organism=Escherichia coli, GI1789087, Length=293, Percent_Identity=44.3686006825939, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI87082152, Length=235, Percent_Identity=48.5106382978723, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI1786524, Length=265, Percent_Identity=46.4150943396226, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI1787583, Length=360, Percent_Identity=36.1111111111111, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI87081872, Length=275, Percent_Identity=40.7272727272727, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI87081858, Length=233, Percent_Identity=33.9055793991416, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1789828, Length=225, Percent_Identity=34.6666666666667, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1789809, Length=111, Percent_Identity=30.6306306306306, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 54190; Mature: 54190

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPIWIVDDDQSIRWVLEKALARDSFATRSFSNVRDALAALDHESPQVLVSDIRMPGGSG
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCC
LELLQAMHERLPGLPVIIMTAFSDLDSAVAAFQGGAFEYLAKPFDVDKAIELIRRAVEES
HHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHH
LRGGVPDDDKLTEAPEMLGQAPAMQDMFRAIGRLSHSAATVLITGESGTGKELVARALHR
HCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHH
HSPRSNGPFIALNTAAIPKDLLESELFGHERGAFTGAQTTRQGRFEQAENGTLFLDEIGD
CCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCHHHCCCCHHCCCCCEEEHHHCC
MPFDLQTRLLRVLSDGQFYRVGGHNPLRANVRVIAATHQNLESRVRQGLFREDLYHRLNV
CCHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHE
IRLRLPPLRERSEDIPLLTKHFLQKSARDLGVEPKRVSDDALAYLSALPFPGNVRQLENL
EEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHH
ANWLTVMAPAQTVEIKDLPPDLGPAQAAPGEGVTPAAVAASTDALPNSATAAVPVPAAPA
HHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCC
ASSNPAWEQGLRSEVARLLRENSADVMDELARRFEAAVIREALDFTRGRKVEAAERLGIG
CCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCC
RNTITRKIQELHLEP
HHHHHHHHHHHCCCC
>Mature Secondary Structure
MKPIWIVDDDQSIRWVLEKALARDSFATRSFSNVRDALAALDHESPQVLVSDIRMPGGSG
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCC
LELLQAMHERLPGLPVIIMTAFSDLDSAVAAFQGGAFEYLAKPFDVDKAIELIRRAVEES
HHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHH
LRGGVPDDDKLTEAPEMLGQAPAMQDMFRAIGRLSHSAATVLITGESGTGKELVARALHR
HCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHH
HSPRSNGPFIALNTAAIPKDLLESELFGHERGAFTGAQTTRQGRFEQAENGTLFLDEIGD
CCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCHHHCCCCHHCCCCCEEEHHHCC
MPFDLQTRLLRVLSDGQFYRVGGHNPLRANVRVIAATHQNLESRVRQGLFREDLYHRLNV
CCHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHE
IRLRLPPLRERSEDIPLLTKHFLQKSARDLGVEPKRVSDDALAYLSALPFPGNVRQLENL
EEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHH
ANWLTVMAPAQTVEIKDLPPDLGPAQAAPGEGVTPAAVAASTDALPNSATAAVPVPAAPA
HHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCC
ASSNPAWEQGLRSEVARLLRENSADVMDELARRFEAAVIREALDFTRGRKVEAAERLGIG
CCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCC
RNTITRKIQELHLEP
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]