Click here to switch to the map view.

The map label for this gene is ptrB [C]

Identifier: 238028046

GI number: 238028046

Start: 2835947

End: 2838055

Strand: Reverse

Name: ptrB [C]

Synonym: bglu_1g24970

Alternate gene names: 238028046

Gene position: 2838055-2835947 (Counterclockwise)

Preceding gene: 238028047

Following gene: 238028044

Centisome position: 72.65

GC content: 72.02

Gene sequence:

>2109_bases
ATGTCCGATTCGCTTCACTGGCCCGCCGGGCCGGATCCGTTCAAGTTCCTCGAATCGCTCGACAGCCGGCGCGCCCGCGC
CTGGGTCGAGGCGCAGAACGCGCGCACGCATGCCGCGCTGCGCGACGACGAGGCCTATCGCGCGCTGGTCGCGCGCCTCG
CGCAGGCCTACCTGCCGCGCGAGCGCCCGGTGATTCCGGCACGCTGGCGCGAGTGGGCCTACGATCTCTGGCAGGACGAC
CGGCATCCGAAGGGGCTCTGGCGCCGCACGCGCTGGGACGACTGGCGCGCCGGCCAGCCGCACTGGGACGTGCTGCTCGA
CGTCGACGCGCTCGGCGCGCACGAGCGCGAGTCCTGGGTGTTCGAGCACGACGCGATCCTCTATCCCGACGGCGACCGCG
CGCTGCTGTCGCTGTCGCCGGGCGGGGCCGATGCGGTCGTGATCCGCGAGTTCGACCTGACGCGGCGCTGCTTCGTGACG
GACGGCTTCACGATCGACTCGCCCGGCAACCACACCATCGACTGGATCGATCGCGACACCGTCTACGTCAGCTGGGAGCG
CGACGCGGCGTCCGTCACCGAAGCCGGCTATCCCTACGAAGTGCGGCGCTGGACACGCGGCACGGCGCTCGCCGAGGCGC
CCGTCGTGTTTCGCGGCGAGCCCGACGACATCAGTGCCGGCGCCGGCTACGATCCGATCGACGAGCGCCACACCGCGTGG
CGCAGCGTCGATTTCTTCGATGTCCATACTTACCGGCTGGCCGCCGACGGCAGCTGGGCGCGCTACGACGTGCCCGCGCA
TGTGGTGGTCGGCTTCTGGCGCGGCTGGCTGATATTGGAGCCGCGCCTCGACTGGGATTGCGAGGGCACGCGGCATGCCG
GCGGCTCGCTGCTGGCAATCCGCGAGGACGCGTTCCTGGCCGGCGCGCGAGGGTTTACGACGCTGTTCGCGCCGGGGCCG
TCCACCTCGGCCTGCACCTGGACCCACACGCAGGGGCTGCTGATCGCGAGCTGGCTCGACGACGTGCGCAACCGCACGCT
GCTCTGGCAGCCCGTGCTGCAGGACGGCGGCGGCTGGCGCTGGGAGTCGCGGCCGTTCGCCTGGCCGAACGAGGCCGAGA
TCGATCTCGAGCCGGTCGAATCGACGCTCGGCGACGAGGTGTTCGTCGACGCCGACACGTTCCTCGATCCGCCAGAGTGC
TGGCTCGCCGATCTCGCGGACCGGGCCGACGATGCCGCCGCGCGCCGCGTGTTGCTCGACCGTCCGCCGGTGCAGTTCGA
CTCGGCCGGGCTCGTGGTGCGGCGCGCCACGGCGCGCTCGCGCGACGGCACCGCGGTGCCCTACACGCTGATCGGTCCGC
GCGATGCGCTCGAGCCGGGCAACGGGGCGGCGCGCACGGCGCGGCCTTGCCTGCTGTCGGGTTACGGCGGCTTTGCGATT
CCCAACCTGCCGGCCTATAGCGACGCGCTCGGCATCGCCTGGCTCGAACGCGGCGGCGTGGCCGCGTTCGCGCACATCCG
CGGCGGCGGCGAATTCGGCTCGCGCTGGCATACCGACGCGCAGCGCGAGCACCGGCAGCGCTCGTTCGACGATTTCATCG
CGGTGGCCGAGGATCTGATCGCGAGCGGCGTGACCACCGCCGCGCAGCTCGGCATCGAGGGCGGCAGCAACGGCGGGCTG
CTGGTGGCCGCCTGCATGGTGCAGCGGCCCGAGCTGTTCGGCGCGGTGCTCTGCCAGGTGCCGCTGCTCGACATGCGACG
CTATCCGAAGCTGCATGCGGGCGCGGCCTGGATCGACGAGTATGGCGATCCCGACGACCCGCTGCAGGGCGCGGCGCTGG
CCGCCTATTCACCGTACCAGCGCGTGCGCGCGGACGTGGTCTACCCGCCGCTGCTGCTGACCACCTCGACGCGCGACGAT
CGCGTCCATCCCGCCCATGCGCGCAAGATGGCCGCGCGCATGCAGTCGCTCGGTCATGAGCAGGTCTGGTACTGGGAGAA
CACCGACGGCGGCCACGGCAGCGCCGACGACCTCGAACGCGCCGAATCGGACGCGGCCGAGTTCGGCTTCCTCTGGACCC
ATCTGGGGCCGGCCCCAGCCAAGGGCTGA

Upstream 100 bases:

>100_bases
CCACCCATTCGGCGGCGGCCCGGCGGCGTCCTTCGCCGCGCTTTCACCGCCTGCCGCACGATCGGACATAATCGGGGTTT
GCCTTCCAGCCGAACCCGAG

Downstream 100 bases:

>100_bases
CGCGGCGCGCGGCCGAACCGGCGCGTGACGGTGCCCGCGCGCGTGTTGCGCACGGGCAGGCTCATTCGACGAGCGCGAAG
ATGGGCGCGTGATCGGACGG

Product: peptidase S9 prolyl oligopeptidase

Products: Hydrolyzed protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 702; Mature: 701

Protein sequence:

>702_residues
MSDSLHWPAGPDPFKFLESLDSRRARAWVEAQNARTHAALRDDEAYRALVARLAQAYLPRERPVIPARWREWAYDLWQDD
RHPKGLWRRTRWDDWRAGQPHWDVLLDVDALGAHERESWVFEHDAILYPDGDRALLSLSPGGADAVVIREFDLTRRCFVT
DGFTIDSPGNHTIDWIDRDTVYVSWERDAASVTEAGYPYEVRRWTRGTALAEAPVVFRGEPDDISAGAGYDPIDERHTAW
RSVDFFDVHTYRLAADGSWARYDVPAHVVVGFWRGWLILEPRLDWDCEGTRHAGGSLLAIREDAFLAGARGFTTLFAPGP
STSACTWTHTQGLLIASWLDDVRNRTLLWQPVLQDGGGWRWESRPFAWPNEAEIDLEPVESTLGDEVFVDADTFLDPPEC
WLADLADRADDAAARRVLLDRPPVQFDSAGLVVRRATARSRDGTAVPYTLIGPRDALEPGNGAARTARPCLLSGYGGFAI
PNLPAYSDALGIAWLERGGVAAFAHIRGGGEFGSRWHTDAQREHRQRSFDDFIAVAEDLIASGVTTAAQLGIEGGSNGGL
LVAACMVQRPELFGAVLCQVPLLDMRRYPKLHAGAAWIDEYGDPDDPLQGAALAAYSPYQRVRADVVYPPLLLTTSTRDD
RVHPAHARKMAARMQSLGHEQVWYWENTDGGHGSADDLERAESDAAEFGFLWTHLGPAPAKG

Sequences:

>Translated_702_residues
MSDSLHWPAGPDPFKFLESLDSRRARAWVEAQNARTHAALRDDEAYRALVARLAQAYLPRERPVIPARWREWAYDLWQDD
RHPKGLWRRTRWDDWRAGQPHWDVLLDVDALGAHERESWVFEHDAILYPDGDRALLSLSPGGADAVVIREFDLTRRCFVT
DGFTIDSPGNHTIDWIDRDTVYVSWERDAASVTEAGYPYEVRRWTRGTALAEAPVVFRGEPDDISAGAGYDPIDERHTAW
RSVDFFDVHTYRLAADGSWARYDVPAHVVVGFWRGWLILEPRLDWDCEGTRHAGGSLLAIREDAFLAGARGFTTLFAPGP
STSACTWTHTQGLLIASWLDDVRNRTLLWQPVLQDGGGWRWESRPFAWPNEAEIDLEPVESTLGDEVFVDADTFLDPPEC
WLADLADRADDAAARRVLLDRPPVQFDSAGLVVRRATARSRDGTAVPYTLIGPRDALEPGNGAARTARPCLLSGYGGFAI
PNLPAYSDALGIAWLERGGVAAFAHIRGGGEFGSRWHTDAQREHRQRSFDDFIAVAEDLIASGVTTAAQLGIEGGSNGGL
LVAACMVQRPELFGAVLCQVPLLDMRRYPKLHAGAAWIDEYGDPDDPLQGAALAAYSPYQRVRADVVYPPLLLTTSTRDD
RVHPAHARKMAARMQSLGHEQVWYWENTDGGHGSADDLERAESDAAEFGFLWTHLGPAPAKG
>Mature_701_residues
SDSLHWPAGPDPFKFLESLDSRRARAWVEAQNARTHAALRDDEAYRALVARLAQAYLPRERPVIPARWREWAYDLWQDDR
HPKGLWRRTRWDDWRAGQPHWDVLLDVDALGAHERESWVFEHDAILYPDGDRALLSLSPGGADAVVIREFDLTRRCFVTD
GFTIDSPGNHTIDWIDRDTVYVSWERDAASVTEAGYPYEVRRWTRGTALAEAPVVFRGEPDDISAGAGYDPIDERHTAWR
SVDFFDVHTYRLAADGSWARYDVPAHVVVGFWRGWLILEPRLDWDCEGTRHAGGSLLAIREDAFLAGARGFTTLFAPGPS
TSACTWTHTQGLLIASWLDDVRNRTLLWQPVLQDGGGWRWESRPFAWPNEAEIDLEPVESTLGDEVFVDADTFLDPPECW
LADLADRADDAAARRVLLDRPPVQFDSAGLVVRRATARSRDGTAVPYTLIGPRDALEPGNGAARTARPCLLSGYGGFAIP
NLPAYSDALGIAWLERGGVAAFAHIRGGGEFGSRWHTDAQREHRQRSFDDFIAVAEDLIASGVTTAAQLGIEGGSNGGLL
VAACMVQRPELFGAVLCQVPLLDMRRYPKLHAGAAWIDEYGDPDDPLQGAALAAYSPYQRVRADVVYPPLLLTTSTRDDR
VHPAHARKMAARMQSLGHEQVWYWENTDGGHGSADDLERAESDAAEFGFLWTHLGPAPAKG

Specific function: Cleaves Peptide Bonds On The C-Terminal Side Of Lysyl And Argininyl Residues. [C]

COG id: COG1505

COG function: function code E; Serine proteases of the peptidase family S9A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S9B family [H]

Homologues:

Organism=Homo sapiens, GI41349456, Length=276, Percent_Identity=37.6811594202899, Blast_Score=180, Evalue=5e-45,
Organism=Homo sapiens, GI284172438, Length=214, Percent_Identity=27.5700934579439, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI284172431, Length=214, Percent_Identity=27.5700934579439, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI108860686, Length=214, Percent_Identity=27.5700934579439, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI284172420, Length=214, Percent_Identity=27.5700934579439, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI284172413, Length=214, Percent_Identity=27.5700934579439, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI70778815, Length=214, Percent_Identity=27.5700934579439, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI108860692, Length=206, Percent_Identity=26.6990291262136, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1788150, Length=287, Percent_Identity=30.3135888501742, Blast_Score=128, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24583414, Length=334, Percent_Identity=35.9281437125749, Blast_Score=195, Evalue=7e-50,
Organism=Drosophila melanogaster, GI221510989, Length=321, Percent_Identity=35.202492211838, Blast_Score=181, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001375
- InterPro:   IPR002470
- InterPro:   IPR004106 [H]

Pfam domain/function: PF00326 Peptidase_S9; PF02897 Peptidase_S9_N [H]

EC number: 3.4.21.83 [C]

Molecular weight: Translated: 78169; Mature: 78038

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSLHWPAGPDPFKFLESLDSRRARAWVEAQNARTHAALRDDEAYRALVARLAQAYLPR
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHCCC
ERPVIPARWREWAYDLWQDDRHPKGLWRRTRWDDWRAGQPHWDVLLDVDALGAHERESWV
CCCCCCHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCEEEEEEHHCCCCCCCCEE
FEHDAILYPDGDRALLSLSPGGADAVVIREFDLTRRCFVTDGFTIDSPGNHTIDWIDRDT
EECCEEEECCCCCEEEEECCCCCCEEEEEECCCCEEEEEECCEEECCCCCCEEEEEECCE
VYVSWERDAASVTEAGYPYEVRRWTRGTALAEAPVVFRGEPDDISAGAGYDPIDERHTAW
EEEEECCCCHHHHCCCCCHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHH
RSVDFFDVHTYRLAADGSWARYDVPAHVVVGFWRGWLILEPRLDWDCEGTRHAGGSLLAI
CCCEEEEEEEEEEECCCCEEEECCCHHHEEEEECCEEEECCCCCCCCCCCCCCCCCEEEE
REDAFLAGARGFTTLFAPGPSTSACTWTHTQGLLIASWLDDVRNRTLLWQPVLQDGGGWR
ECCCEEECCCCCEEEECCCCCCCCEEEECCCCEEHHHHHHHHHCCEEEEEHHHHCCCCCC
WESRPFAWPNEAEIDLEPVESTLGDEVFVDADTFLDPPECWLADLADRADDAAARRVLLD
CCCCCCCCCCCCCEEHHHHHHHCCCEEEEEHHCCCCCHHHHHHHHHHCCHHHHHHHHHHC
RPPVQFDSAGLVVRRATARSRDGTAVPYTLIGPRDALEPGNGAARTARPCLLSGYGGFAI
CCCCCCCCCCEEEEEECCCCCCCCCCCEEEECCHHCCCCCCCCCCCCCCEEEECCCCCCC
PNLPAYSDALGIAWLERGGVAAFAHIRGGGEFGSRWHTDAQREHRQRSFDDFIAVAEDLI
CCCCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ASGVTTAAQLGIEGGSNGGLLVAACMVQRPELFGAVLCQVPLLDMRRYPKLHAGAAWIDE
HHCCHHHHHHCCCCCCCCCEEEEEHHHHCHHHHHHHHHHCCHHHHHCCCCHHCCHHHHHH
YGDPDDPLQGAALAAYSPYQRVRADVVYPPLLLTTSTRDDRVHPAHARKMAARMQSLGHE
CCCCCCCCCCCEEECCCCHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCC
QVWYWENTDGGHGSADDLERAESDAAEFGFLWTHLGPAPAKG
EEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCC
>Mature Secondary Structure 
SDSLHWPAGPDPFKFLESLDSRRARAWVEAQNARTHAALRDDEAYRALVARLAQAYLPR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHCCC
ERPVIPARWREWAYDLWQDDRHPKGLWRRTRWDDWRAGQPHWDVLLDVDALGAHERESWV
CCCCCCHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCEEEEEEHHCCCCCCCCEE
FEHDAILYPDGDRALLSLSPGGADAVVIREFDLTRRCFVTDGFTIDSPGNHTIDWIDRDT
EECCEEEECCCCCEEEEECCCCCCEEEEEECCCCEEEEEECCEEECCCCCCEEEEEECCE
VYVSWERDAASVTEAGYPYEVRRWTRGTALAEAPVVFRGEPDDISAGAGYDPIDERHTAW
EEEEECCCCHHHHCCCCCHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHH
RSVDFFDVHTYRLAADGSWARYDVPAHVVVGFWRGWLILEPRLDWDCEGTRHAGGSLLAI
CCCEEEEEEEEEEECCCCEEEECCCHHHEEEEECCEEEECCCCCCCCCCCCCCCCCEEEE
REDAFLAGARGFTTLFAPGPSTSACTWTHTQGLLIASWLDDVRNRTLLWQPVLQDGGGWR
ECCCEEECCCCCEEEECCCCCCCCEEEECCCCEEHHHHHHHHHCCEEEEEHHHHCCCCCC
WESRPFAWPNEAEIDLEPVESTLGDEVFVDADTFLDPPECWLADLADRADDAAARRVLLD
CCCCCCCCCCCCCEEHHHHHHHCCCEEEEEHHCCCCCHHHHHHHHHHCCHHHHHHHHHHC
RPPVQFDSAGLVVRRATARSRDGTAVPYTLIGPRDALEPGNGAARTARPCLLSGYGGFAI
CCCCCCCCCCEEEEEECCCCCCCCCCCEEEECCHHCCCCCCCCCCCCCCEEEECCCCCCC
PNLPAYSDALGIAWLERGGVAAFAHIRGGGEFGSRWHTDAQREHRQRSFDDFIAVAEDLI
CCCCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ASGVTTAAQLGIEGGSNGGLLVAACMVQRPELFGAVLCQVPLLDMRRYPKLHAGAAWIDE
HHCCHHHHHHCCCCCCCCCEEEEEHHHHCHHHHHHHHHHCCHHHHHCCCCHHCCHHHHHH
YGDPDDPLQGAALAAYSPYQRVRADVVYPPLLLTTSTRDDRVHPAHARKMAARMQSLGHE
CCCCCCCCCCCEEECCCCHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCC
QVWYWENTDGGHGSADDLERAESDAAEFGFLWTHLGPAPAKG
EEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+ [C]

Kcat value (1/min): 11820 [C]

Specific activity: NA

Km value (mM): 0.23 {tosyl-Arg} 0.33 {N-benzyloxycarbonyl-Lys} 0.31 {N-benzyloxycarbonyl-Lys} 0.92 {benzoyl-Lys} 0.6 {N-benzoyl-Arg} 0.5 {benzoyl-Arg} 0.48 {benzoyl-Arg} 0.25 {benzoyl-Arg} 80 {acetyl-tyrosine} 0.47 {tosyl-Lys-methyl} [C]

Substrates: Protein; H2O [C]

Specific reaction: Protein + H2O = hydrolyzed protein [C]

General reaction: Peptide bond hydrolysis [C]

Inhibitor: Antipain; Aromaticamidines; Benzamidine; Co2+; DFP; Fe2+; Hg2+; L-Arginine; Leupeptin sulfhydryl agents, trypsin inhibitors, 1, 10-phenanthroline; p-Aminobenzamidine; Tosyl -Leuchloromethyl ketone; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]